LemaA.20996.a

CULT domain-containing protein

CENTER ID: LemaA.20996.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.20996.a.A1.GE45904 full length 1 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.09.0570
UniProt: Q4QHW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSASPSSPST AAASASPQDT VEVLLCDSCR CPIGSLWDVL PAEEAVPVWR DQVYSYELDL FANGSPPIQA YSATNPSARR FDLLRLAPYV TLHRVSTPRG EAPPPGADAS RSCNAGASTT AASALDAQEC EGSVQVEEVS TAHAPPADAT GTRGAASTTL HAPSFVECHT TVYSTEHSFF AGYAWCFCNC SNCGAFLGWG FAAVDRLRAA RRLHRGGKSG EVSETGDVGR PRKKGARQEE EVRKTETDIA TAAAAGDSQP AESSSPPAAP SAPRDQHQQR PRGEGEGCRS GAHASDLAPP ADSDDSSDEG TSMMTTSDSN DGDDSSAIGG GGGARGGGQE HTNTGVTPDF IGIIITHCTS DPDYPIASLM RDVEWRAARQ RRRKRVEALT RRISLLLPQM RDSYRAHEIY HDYHAMEQLL YAAPLLPLQD VSDSFTTAVT ALLSDVDEDG VPIRMHATVE AARIAVARQM DSDNSSGVSG HSSSGHEGNS PS
NT Sequence
atgagcgcat cgccttcctc cccgtccacg gctgccgcct ccgccagccc gcaggacacc gtcgaagtcc ttctctgtga cagctgtcgt tgccctattg gttcgctgtg ggatgtgctg ccggcagagg aggcggttcc ggtgtggagg gatcaggtgt acagctacga gctggacctt tttgccaatg gatcgccgcc gatacaggcg tacagcgcca cgaacccgag cgcgcggcgg tttgatctgc tgcgcttggc tccgtacgtg acactgcatc gggtctcaac gcctcgtggc gaagctccac cacccggtgc cgatgcttct cgctcgtgta acgccggtgc ttcgactact gccgcctcgg ccctcgatgc acaggagtgt gaaggatcag tgcaggtcga ggaggtctcc acagcgcatg cgccaccggc ggatgcgaca gggacgcgcg gtgccgcctc caccacgctt cacgcgccgt cttttgtgga gtgccacacg accgtctact ccaccgaaca ctccttcttc gccggctatg cgtggtgctt ctgcaactgc agcaactgtg gtgccttcct ggggtggggg tttgcggcgg tggatcgcct gcgcgccgca cgacgccttc atcggggcgg caagagcggc gaagtgtccg agaccggcga tgtcggtcgg ccacgcaaga agggggcgcg ccaggaggaa gaggtgagaa aaaccgagac ggacattgcc acagccgcag ctgcgggcga cagccaacct gccgaatcaa gttccccgcc cgcagcgccg agtgcccctc gggatcagca ccagcaacgg ccacgtgggg aaggcgaagg ctgcagaagc ggcgcacacg ctagtgatct cgcgccgcca gctgactccg acgatagcag tgatgaaggc acgtctatga tgaccaccag tgacagcaac gacggcgacg acagcagcgc catcggcggc ggcggcggtg ctcgaggtgg tgggcaagag catacaaaca ccggcgtgac gcccgacttc atcggcatca tcattaccca ttgcaccagc gatccagact acccgatagc gtccctcatg cgcgatgtcg agtggcgtgc cgcgcggcaa cgacgccgca agcgagttga agccctgaca cgccgcatct ccctcctcct cccacagatg agggacagct accgcgcgca cgagatttac cacgactacc acgccatgga gcagttgctt tacgccgcac cgcttctccc gctgcaggat gtatcagata gcttcacgac cgcggtaacc gcactcctgt ccgatgtgga cgaggacggg gtgccgattc gcatgcacgc cacggtggaa gcagcacgca tcgccgtagc gaggcagatg gacagcgaca acagcagtgg tgtaagcgga cacagcagca gcgggcacga gggaaacagt ccctcgtag
Details for LemaA.20996.a.A1.GE45904
HARVESTED ON: 5/13/2025
SEQUENCED ON: data unavailable
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSASPSSPS TAAASASPQD TVEVLLCDSC RCPIGSLWDV LPAEEAVPVW RDQVYSYELD LFANGSPPIQ AYSATNPSAR RFDLLRLAPY VTLHRVSTPR GEAPPPGADA SRSCNAGAST TAASALDAQE CEGSVQVEEV STAHAPPADA TGTRGAASTT LHAPSFVECH TTVYSTEHSF FAGYAWCFCN CSNCGAFLGW GFAAVDRLRA ARRLHRGGKS GEVSETGDVG RPRKKGARQE EEVRKTETDI ATAAAAGDSQ PAESSSPPAA PSAPRDQHQQ RPRGEGEGCR SGAHASDLAP PADSDDSSDE GTSMMTTSDS NDGDDSSAIG GGGGARGGGQ EHTNTGVTPD FIGIIITHCT SDPDYPIASL MRDVEWRAAR QRRRKRVEAL TRRISLLLPQ MRDSYRAHEI YHDYHAMEQL LYAAPLLPLQ DVSDSFTTAV TALLSDVDED GVPIRMHATV EAARIAVARQ MDSDNSSGVS GHSSSGHEGN SPS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcatcg ccttcctccc cgtccacggc tgccgcctcc gccagcccgc aggacaccgt cgaagtcctt ctctgtgaca gctgtcgttg ccctattggt tcgctgtggg atgtgctgcc ggcagaggag gcggttccgg tgtggaggga tcaggtgtac agctacgagc tggacctttt tgccaatgga tcgccgccga tacaggcgta cagcgccacg aacccgagcg cgcggcggtt tgatctgctg cgcttggctc cgtacgtgac actgcatcgg gtctcaacgc ctcgtggcga agctccacca cccggtgccg atgcttctcg ctcgtgtaac gccggtgctt cgactactgc cgcctcggcc ctcgatgcac aggagtgtga aggatcagtg caggtcgagg aggtctccac agcgcatgcg ccaccggcgg atgcgacagg gacgcgcggt gccgcctcca ccacgcttca cgcgccgtct tttgtggagt gccacacgac cgtctactcc accgaacact ccttcttcgc cggctatgcg tggtgcttct gcaactgcag caactgtggt gccttcctgg ggtgggggtt tgcggcggtg gatcgcctgc gcgccgcacg acgccttcat cggggcggca agagcggcga agtgtccgag accggcgatg tcggtcggcc acgcaagaag ggggcgcgcc aggaggaaga ggtgagaaaa accgagacgg acattgccac agccgcagct gcgggcgaca gccaacctgc cgaatcaagt tccccgcccg cagcgccgag tgcccctcgg gatcagcacc agcaacggcc acgtggggaa ggcgaaggct gcagaagcgg cgcacacgct agtgatctcg cgccgccagc tgactccgac gatagcagtg atgaaggcac gtctatgatg accaccagtg acagcaacga cggcgacgac agcagcgcca tcggcggcgg cggcggtgct cgaggtggtg ggcaagagca tacaaacacc ggcgtgacgc ccgacttcat cggcatcatc attacccatt gcaccagcga tccagactac ccgatagcgt ccctcatgcg cgatgtcgag tggcgtgccg cgcggcaacg acgccgcaag cgagttgaag ccctgacacg ccgcatctcc ctcctcctcc cacagatgag ggacagctac cgcgcgcacg agatttacca cgactaccac gccatggagc agttgcttta cgccgcaccg cttctcccgc tgcaggatgt atcagatagc ttcacgaccg cggtaaccgc actcctgtcc gatgtggacg aggacggggt gccgattcgc atgcacgcca cggtggaagc agcacgcatc gccgtagcga ggcagatgga cagcgacaac agcagtggtg taagcggaca cagcagcagc gggcacgagg gaaacagtcc ctcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa