LemaA.20384.a

Protein kinase domain-containing protein

CENTER ID: LemaA.20384.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.20384.a.HZ2.GE43724 kinase domain (Jensen) 1 511
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.21.0270
UniProt: Q4QCI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATVDTVRGR YSSYKLLKEV GRGGSALVYR AQDVATRKDV AVKQLFGNRP QDMEDWLREV DALHTLNSHH CPHVVKYLDH MQQHDRLFLV EEFTEHGSLL RCLKENKKLP EDTACRYIYQ VLTALYHMAP WGVVHGDLKA SNILLFDNDV VKLTDFALRS HGEDDHEDAE RRTTAASTAS AEALPPMRGS RAGRDAGEEC SGSALSEFRG SAYWAAPEIL AGASKATAAS DIWSVGCLAV ELLTGAPPYF ERPVYNAIYH ILKSYYEVLC ESEASRVGAG GTSCASTNPA TPSAATGSGD DDGPRGPRKK KNGAKDAGGA PSQKTDVASA RRATTTTSTR NPPKEPVDAA KTAASSGAVS ASEQNEVATP LCEAALLPPL PEDLHVSDEC LSFLRMCFRP RAVDRPSAGE LLHDLWFLDC TVPQLLRAAR EGRPMSGTTG DESVSAGTSS GSRFGVIVQW VKRNLTCDNE SRCKAWLNSD ALPLLVPVLT PRIMTPKYIG DVMRCFSHFA ESRSALATLF VNRLGSTELW GVEELTSACD ADHLATLFRR CCATQDAQVP VYTPTDRRAL RFVLGLEKEK ALACVRALHS RLVLEPTAAA MAAAASSPTT ATDDTAVDLN DCSASSRDPA EPAHARAPDC VPASLQEQHE QQRRARERLL SDGGAAVLCQ CVEAQCKAAF LSNTAPMMEW STMNLLFDVL CTIEPLAGGQ ALLWGLRADL SGGHTSPSST VGPGGGGAAM VKSLTVVVNP CGGRGGGELN STWDKSLSGT GPPLSVSPIS VSPQELIPAT AAAAGGGGGL LSSLGSTMRL GCHTVSGLPP DSVQWATSMS WLLAVQEAAR HLCEAAGRLL TRYIPVARRC RAEYLEKAGV SLTATLLLVA SSEVVSTEVR CAAVEGLPQL QASSLRAARY LRDPVRCIAL LALTLKRSFG APALTSCLLT AITTMTSEKQ MLAACTGCPW VWEALVSLLR EVEEADKAAA AGGKRSSSAL HSAASTDGGA AEGADAPPAL AAAAASPTSV KSPSASSSSG SGSNSAAAVF ADIVVLLSRW FAEVTPTALL SSAVTAAAAA SEAILALGQP AASPPLPVLL QTLRCQLIAL SQNDRVCHGD LMPDVAKALR HLAPLEAAAV AASTSTATVA
NT Sequence
atggccaccg tagacaccgt cagggggcgg tactcgtcct acaagctgct caaggaagtt ggccgcggtg gcagcgcgct cgtgtaccgc gcgcaggacg tggccacacg caaggatgtt gccgtgaagc agctctttgg caaccggccg caggatatgg aagactggct gcgcgaggtg gacgccctac acacgctcaa cagccatcat tgcccgcatg tcgtgaagta cctggaccac atgcagcagc acgatcgact cttcctcgtg gaggagttca ccgagcacgg atcgctgctg cgctgtctga aggaaaacaa aaagcttcca gaggacaccg cttgccggta tatctaccag gtgctgacgg ccctgtatca catggcccca tggggcgtcg tgcacggcga cttgaaggcg tccaacattc ttctcttcga caacgatgtc gtgaaactga cggactttgc actgcgctcg catggcgagg acgaccacga ggatgccgag cggcgcacca ccgcggcctc cacggcttct gccgaagcac tgccccccat gaggggcagc cgcgccggga gggacgctgg tgaagagtgc tctggctccg ccctctcgga gttccgcggc tccgcttact gggcagcgcc ggaaatcctc gccggtgcct cgaaggcaac ggcggcgagc gacatctggt cggtgggctg cctcgcagtg gagttgctca cgggcgcccc gccgtacttc gagcgccccg tctacaacgc catttatcac atcctcaaga gctactacga ggtgctctgt gaaagcgagg ccagccgcgt cggtgctggc ggcacctcgt gcgcgtccac caaccctgca accccaagcg cggcaacggg ctctggcgac gatgacgggc cgcgtgggcc ccggaaaaag aaaaacggag cgaaagatgc gggtggcgca ccgtcacaga agacagacgt ggcgtccgcg cggagggcga cgacaacgac gagcacccgc aatccgccga aggagccggt cgatgcggcc aagacggccg catctagcgg cgctgtgagt gcatcagagc agaatgaggt ggccacgcct ctgtgcgagg cggcgctgct cccaccgctt cccgaggacc tgcacgtgag cgatgagtgt ctgtcgtttc tacggatgtg cttccgcccc cgcgcggtgg accggccgtc ggctggggag ctgctccacg acttgtggtt cctggactgc accgtaccgc agctcctgcg ggcagcgcgc gagggtcggc ctatgagcgg gacgacaggc gacgagagcg tcagcgccgg caccagcagc ggcagccgct ttggggtgat tgtgcagtgg gtgaagcgca acctgacatg tgataacgag agccggtgca aggcgtggct gaacagcgac gcgctgccgc tgctggtacc ggtgctgacg ccgcgtatta tgacacccaa gtacattgga gacgtaatgc gctgcttttc ccactttgcc gagagccgct ccgccttggc gacgctcttt gtgaaccggt tggggtcgac agaactctgg ggggtggagg agctgacgtc ggcgtgcgac gcggaccacc tcgccacact cttccggcgg tgctgtgcca cgcaggacgc gcaggtgccc gtgtacaccc ccacagaccg gcgtgcgctg cgtttcgtct taggcctgga gaaggagaag gctctggcgt gtgtgcgggc gttgcactcg cgtctcgtgt tggagccgac ggccgcggcg atggctgcgg ctgcgagctc gccgaccacc gccacagacg acaccgcagt ggatctgaac gactgctccg cctcctcgcg tgaccccgcg gagcccgcgc atgcgcgggc gccggattgc gtaccggcat cccttcaaga gcagcatgag cagcagcgac gtgcccgtga gcggctactc agcgacggcg gcgcggcggt gctgtgccag tgcgtggagg cgcagtgcaa ggcagctttc ctgagcaaca cagcgcccat gatggagtgg agcacaatga acctgctgtt tgacgttctc tgcaccatcg agccgctggc gggcgggcag gcgctactgt gggggctccg cgctgacctc agcggtggcc acacctcccc gagctctact gtgggcccag gtggtggtgg tgcggcgatg gtgaaatcgc tgacggttgt cgtcaacccg tgcggaggac gcggcggcgg cgagttgaac agcacctggg acaagtcgtt gtcagggacg ggtcccccgc tttcggtgtc accgatctcg gtgagcccgc aggagttgat cccggcgaca gcggcagcgg cgggcggtgg tggcggtctg ctgagctccc taggcagcac tatgaggctc ggatgccaca ccgtcagcgg gcttccaccg gattcagtgc agtgggccac atccatgtca tggctgctcg cagtgcaaga ggcggcgcgc cacctgtgcg aggcagcggg gcgcctgctc acccgctaca tcccagtcgc cagaaggtgc agggccgagt acctcgagaa ggctggtgtc agtctgactg caacgctgct cctcgtggcc tcgagcgagg tggtcagcac cgaggtgcga tgcgcggctg tcgaaggcct gccgcagctg caggcgagct cgctccgtgc ggcccggtac ctgcgcgacc cggtgcgctg cattgccctg ctggcgctca ccctgaagcg gtccttcggt gcccctgcgc tgacgtcgtg cctgctcaca gccatcacca ccatgacgtc cgagaagcag atgctagcgg cctgcaccgg ttgcccgtgg gtgtgggagg cactggtgtc cctgctaaga gaagtggagg aagctgacaa agccgccgcc gctggtggca agcgcagcag ttcggctctc cacagcgctg ccagcaccga cggtggcgcg gccgaggggg ccgacgcccc acctgcctta gcagcagcgg cggcgagtcc cacatcagtc aaatcgccta gcgccagcag cagcagcggc agtggcagca acagcgccgc tgcggttttt gccgacattg ttgtgcttct ctcgcgttgg ttcgcggagg tgacaccgac ggcgctgctg agcagtgccg taacagcagc agcagcggca tcggaagcaa tacttgccct cggtcagcca gcagcgtccc cgcccctccc ggtgctgctg cagacacttc gctgccaact cattgcgctg tcgcagaacg atcgtgtctg ccacggtgat ctgatgccgg atgtggccaa ggcgctgcgg catttggccc ctctcgaagc cgccgccgtc gccgcgtcaa cgtcgacggc cacagtggcg tga
Details for LemaA.20384.a.HZ2.GE43724
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA TVDTVRGRYS SYKLLKEVGR GGSALVYRAQ DVATRKDVAV KQLFGNRPQD MEDWLREVDA LHTLNSHHCP HVVKYLDHMQ QHDRLFLVEE FTEHGSLLRC LKENKKLPED TACRYIYQVL TALYHMAPWG VVHGDLKASN ILLFDNDVVK LTDFALRSHG EDDHEDAERR TTAASTASAE ALPPMRGSRA GRDAGEECSG SALSEFRGSA YWAAPEILAG ASKATAASDI WSVGCLAVEL LTGAPPYFER PVYNAIYHIL KSYYEVLCES EASRVGAGGT SCASTNPATP SAATGSGDDD GPRGPRKKKN GAKDAGGAPS QKTDVASARR ATTTTSTRNP PKEPVDAAKT AASSGAVSAS EQNEVATPLC EAALLPPLPE DLHVSDECLS FLRMCFRPRA VDRPSAGELL HDLWFLDCTV PQLLRAAREG RPMSGTTGDE SVSAGTSSGS RFGVIVQWVK RNLTCDNESR CKAWLNSDAL PLLVPVLTPR IMTPKYIGDV MRCFSHFAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggccaccgt agacaccgtc agggggcggt actcgtccta caagctgctc aaggaagttg gccgcggtgg cagcgcgctc gtgtaccgcg cgcaggacgt ggccacacgc aaggatgttg ccgtgaagca gctctttggc aaccggccgc aggatatgga agactggctg cgcgaggtgg acgccctaca cacgctcaac agccatcatt gcccgcatgt cgtgaagtac ctggaccaca tgcagcagca cgatcgactc ttcctcgtgg aggagttcac cgagcacgga tcgctgctgc gctgtctgaa ggaaaacaaa aagcttccag aggacaccgc ttgccggtat atctaccagg tgctgacggc cctgtatcac atggccccat ggggcgtcgt gcacggcgac ttgaaggcgt ccaacattct tctcttcgac aacgatgtcg tgaaactgac ggactttgca ctgcgctcgc atggcgagga cgaccacgag gatgccgagc ggcgcaccac cgcggcctcc acggcttctg ccgaagcact gccccccatg aggggcagcc gcgccgggag ggacgctggt gaagagtgct ctggctccgc cctctcggag ttccgcggct ccgcttactg ggcagcgccg gaaatcctcg ccggtgcctc gaaggcaacg gcggcgagcg acatctggtc ggtgggctgc ctcgcagtgg agttgctcac gggcgccccg ccgtacttcg agcgccccgt ctacaacgcc atttatcaca tcctcaagag ctactacgag gtgctctgtg aaagcgaggc cagccgcgtc ggtgctggcg gcacctcgtg cgcgtccacc aaccctgcaa ccccaagcgc ggcaacgggc tctggcgacg atgacgggcc gcgtgggccc cggaaaaaga aaaacggagc gaaagatgcg ggtggcgcac cgtcacagaa gacagacgtg gcgtccgcgc ggagggcgac gacaacgacg agcacccgca atccgccgaa ggagccggtc gatgcggcca agacggccgc atctagcggc gctgtgagtg catcagagca gaatgaggtg gccacgcctc tgtgcgaggc ggcgctgctc ccaccgcttc ccgaggacct gcacgtgagc gatgagtgtc tgtcgtttct acggatgtgc ttccgccccc gcgcggtgga ccggccgtcg gctggggagc tgctccacga cttgtggttc ctggactgca ccgtaccgca gctcctgcgg gcagcgcgcg agggtcggcc tatgagcggg acgacaggcg acgagagcgt cagcgccggc accagcagcg gcagccgctt tggggtgatt gtgcagtggg tgaagcgcaa cctgacatgt gataacgaga gccggtgcaa ggcgtggctg aacagcgacg cgctgccgct gctggtaccg gtgctgacgc cgcgtattat gacacccaag tacattggag acgtaatgcg ctgcttttcc cactttgccg agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa