LemaA.20227.a

protein kinase, putative (OPH8)

CENTER ID: LemaA.20227.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.20227.a.B2.GE42809 PHoranyi kinase domain truncation 452 755
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.25.1520
UniProt: Q4Q9T9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPSSKRHAY EPLPQQQQQH PTNPPAAYQH RRVSTGTGRD SPALPQLSPN SMPAMTNIAE PSSSPALIST SAASSTLVPI FQGREGSVAA SRGGGSGNSE AHPCPAEESH SYPGASTTAA QSMALAVVPF TGTSSGGMSS DGVGVSAPQR CDPRVGGVVQ EGKNTGVGGD DVGYYSGSSP YGKLAPLSLS TERVCPHCLR PYDVFDDREG VLDPATFFRV DATTRSSSIQ PSPSHPKLLP PPSQEGDEQQ KQQPSKPPRL YTKSAGDGGS GTGQLLCSRG SMTAGLDDSS SDGINRSGNY TPVHPPAAAF TSHYFRALPP PSFIIAAGAR GASAPLAIED YRPSPLLDSP RGEKEEARQP FALAPASASD NGGGGGNSSG TLVCKVAPVP HYSYVWPITC TGMRSQVRLL QQGDGSDKTA AEVEGRKEES PSCESGSPVC SPTAATPSPN NGYYRHYFKE LRQLGRGTYG GVYLCRHIMC GVNLGEFALK KIPVGDKVTY LQSVLREVRI LEEVRRHPNV VEYKHSWVEE AQLADFGPPV RCLFILMEYA SAGSLDTYLE RYGNNLSTLA VWYFFLSSVA GTAHLHEKHI LHRDLKPQNL LLAATKDRPP RVLVSDFGTA ALLDDILYDR SGGTGTLEYM APELLETTTS LRGTNERYVN HHTMASDVWS LGMVLHYLAF DGALPERHED GSVNLDKARH SANARPPEML RLLEAMLQLD PAKRPRCNDI LGSPMVQSIM RIFNKDDHSQ WDLSIQRQQQ LSATSPSAIL PLERPPLPPV PAPAPRSTPL NVLEAHSIDD SSSPRGARSR NAILAVSTSS QGHGARADRT NLEDKSNTLQ SSRVELLSAS TLVDLSTAMA LVGSSSSSNG SPFAVKLPPP RPPSTVISQH GRQQQQRAHA VVGLPQTLAS FNTSQLSDSP SPRRVNVGVQ TNPVVIVEKQ QRDT
NT Sequence
atgaacccgt cgtcgaagcg gcacgcgtat gagccgctgc cgcagcagca gcaacagcac cccacaaacc cgccggctgc ttatcagcat cgccgagtaa gcacaggcac tggtcgcgac agccctgccc ttccacagct ctcgccgaac tcgatgcctg cgatgaccaa catcgctgag ccctcctcaa gccccgccct catctccacc tcggcagcgt cgtcaactct ggtgcccatc ttccaagggc gggagggttc tgtagcagct tcaagaggtg gcggcagcgg caattctgaa gctcatcctt gtcctgccga ggaaagccac agctaccccg gggcgtctac cacggcggcg cagtccatgg cgctggcggt ggtgcccttc acaggcacta gcagcggtgg gatgagcagt gacggcgtcg gcgtcagtgc accgcaacgg tgtgacccga gagtgggtgg tgtcgtccaa gaaggcaaga acacaggtgt tggcggtgat gacgttggct actactctgg gtcctccccg tatggcaagc tggccccgct gtcgctgagc acggagcgag tgtgtccgca ctgccttcgg ccgtacgacg tcttcgacga ccgcgaaggc gtattggacc cggcgacgtt cttccgcgtg gatgcaacaa cgaggtcgtc gtcgatacag ccctcaccaa gccatccgaa gctgctcccg ccgccgtcgc aggaggggga tgagcagcag aagcagcaac catcgaagcc cccgcgctta tacactaaga gcgcagggga tggtggcagt ggtactgggc agctgttgtg cagcaggggc agcatgaccg ctgggctcga tgacagcagc agtgacggca tcaaccgcag cggcaactac acgcccgtac acccaccagc cgctgcgttc acctcgcact acttccgtgc gctgccccca ccctcgttca tcatagcggc gggagcccgc ggtgcatcgg caccgctcgc catcgaggac taccgcccat cgccgctgct cgatagcccg agaggcgaga aggaggaagc gagacagccg ttcgcactgg ctcccgcgtc tgccagtgac aacggtggtg gaggtggtaa ctcgagtggg acgctggtgt gcaaagtagc tcccgtgccg cactactcct acgtgtggcc catcacctgc acaggaatgc gctcgcaggt gcgactgctg cagcagggtg acggcagcga caagacggcg gcagaggtgg agggcaggaa agaggagtcg ccgtcgtgcg agagcggctc gcccgtctgc tcccccaccg ctgcgacgcc gtcgccgaac aatggctact accgtcatta cttcaaggag ctccgtcagc tgggtcgcgg cacctacgga ggagtctacc tctgtcgcca cattatgtgt ggcgttaacc tcggcgagtt cgctcttaag aagattccag tcggcgacaa ggtcacctac ctgcagtcgg tgctgcgtga ggtgcgcatt ctcgaggagg tccgacggca ccccaacgtg gtcgagtaca agcacagctg ggtggaggag gcgcagctcg ccgactttgg tccgcccgtg cgctgtctct tcatcttgat ggagtacgcg tccgcaggct cgctcgacac gtatctggag cgctacggca acaatctctc caccctcgcc gtgtggtact tcttcctcag ctccgtggca ggcacggcgc accttcacga gaagcacatc ttgcaccgcg acctaaagcc tcagaacctg ttgctggccg caacgaagga ccgtccgcca cgggtgctgg tcagcgactt tggcacggcg gcgctgctgg atgacatcct gtacgaccgg tccggtggca cgggcacgct cgagtacatg gcgccggagc tcctcgagac gaccacctcg cttcgcggca ccaatgagcg gtacgtgaac catcacacga tggcctccga cgtgtggtcg ctggggatgg tgctgcacta cctcgccttc gacggcgcgc tgccggagcg acacgaggac ggcagtgtca acctcgacaa ggcacgccac tcggcgaacg ccagaccgcc tgaaatgctc agactgctgg aggccatgct gcagcttgat cccgcaaagc ggccgcgctg caacgacatc ctcggctccc cgatggtgca gtccatcatg cgcatcttca acaaagatga ccatagccag tgggacctct ccatccagcg gcagcagcag ctctcggcta cctcgccgtc tgcgattctg ccgttggagc gtccccctct cccaccggtg ccggcgccag cgcccaggag tacaccactc aatgttctag aggcccacag catcgatgac agcagctcgc cccgcggcgc tcgcagccgc aacgctatcc tcgccgtctc cacctcttca cagggacatg gcgcgcgggc cgaccgtacg aatctggagg acaagagcaa cacacttcaa agcagccgtg tcgagctcct ctcagcgtcg acgttggtgg atctgtcaac ggcgatggcg cttgtcggga gcagcagcag cagcaacggc agccccttcg cagtgaagtt gcctccgccg cgccctccat ctactgtgat ctcccagcat ggccgacagc agcagcaacg cgcgcatgcg gtggtcgggt taccgcagac cctagcctcc ttcaacacct cccagctttc agactcgcca tcgccgcgca gggtcaacgt cggcgtgcaa accaatccgg tggtgatagt ggagaagcag caacgggaca cataa
Details for LemaA.20227.a.B2.GE42809
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 81
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMG YYRHYFKELR QLGRGTYGGV YLCRHIMCGV NLGEFALKKI PVGDKVTYLQ SVLREVRILE EVRRHPNVVE YKHSWVEEAQ LADFGPPVRC LFILMEYASA XXLDTYLERY GNNLSTLAVW YFFLSSVAGT AHLHEKHILH RDLKPQNLLL AATKDRPPRV LVSDFGTAAL LDDILYDRSG GTGTLEYMAP ELXXTTTSXX XTXXRYVNHH TMASDVXXLG XXXHXXAFDG ALPERQEXGS VXXXXARHSA NXXPXEMLXX LXXXXQXDPA KRXXXXXXXX XXXXXXIXXX XNKXXXXXXD XXXXVS
Validated NT Sequence
ttactcactt nnnnngnnng gtccnnnnnn nnnnnnnnnn ntttgttgan nnnnnnnnng atgnnntnnn nnntnnnnna gnnnnnnnnn nnnntgcnnn nnnnncgctt tgcgggatca nnctgcnnna tnnnntccag cannnnnagc atttcnngcg gtcnnntgtt cgccgagtgg cgtgcnnnnn nnnnnttgac actgccntcc tcctgtcgct ccggcagcgc gccgtcgaag gcgnnntagt gcannncnnt ccccagcnnn nacacgtcgg aggccatcgt gtgatggttc acgtaccgcn nnnnngtgcn nngnaacgag gtggtcgtct nnnggagctc cggcgccatg tactcgagcg tgcccgtgcc accggaccgg tcgtacagga tgtcatccag cagcgccgcc gtnccaaagt cgctgaccag cacccgtggc ggacggtcct tcgttgcggc cagcaacagg ttctgaggct ttaggtcgcg gtgcaagatg tgcttctcgt gaaggtgcgc cgtgcctgcc acggagctga ggaagaagta ccacacggcg agggtggaga gattgttgcc gtagcgctcc agatacgtgt cgagcgnnnn tgcggacgcg tactccatca agatgaagag acagcgcacg ggcggaccaa agtcggcgag ctgcgcctcc tccacccagc tgtgcttgta ctcgaccacg ttggggtgcc gtcggacctc ctcgagaatg cgcacctcac gcagcaccga ctgcaggtag gtgaccttgt cgccgactgg aatcttctta agagcgaact cgccgaggtt aacgccacac ataatgtggc gacagaggta gactcctccg taggtgccgc gacccagctg acggagctcc ttgaagtaat gacggtagta gcccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHGY YRHYFKELRQ LGRGTYGGVY LCRHIMCGVN LGEFALKKIP VGDKVTYLQS VLREVRILEE VRRHPNVVEY KHSWVEEAQL ADFGPPVRCL FILMEYASAG SLDTYLERYG NNLSTLAVWY FFLSSVAGTA HLHEKHILHR DLKPQNLLLA ATKDRPPRVL VSDFGTAALL DDILYDRSGG TGTLEYMAPE LLETTTSLRG TNERYVNHHT MASDVWSLGM VLHYLAFDGA LPERHEDGSV NLDKARHSAN ARPPEMLRLL EAMLQLDPAK RPRCNDILGS PMVQSIMRIF NKDDHSQWDL SI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctact accgtcatta cttcaaggag ctccgtcagc tgggtcgcgg cacctacgga ggagtctacc tctgtcgcca cattatgtgt ggcgttaacc tcggcgagtt cgctcttaag aagattccag tcggcgacaa ggtcacctac ctgcagtcgg tgctgcgtga ggtgcgcatt ctcgaggagg tccgacggca ccccaacgtg gtcgagtaca agcacagctg ggtggaggag gcgcagctcg ccgactttgg tccgcccgtg cgctgtctct tcatcttgat ggagtacgcg tccgcaggct cgctcgacac gtatctggag cgctacggca acaatctctc caccctcgcc gtgtggtact tcttcctcag ctccgtggca ggcacggcgc accttcacga gaagcacatc ttgcaccgcg acctaaagcc tcagaacctg ttgctggccg caacgaagga ccgtccgcca cgggtgctgg tcagcgactt tggcacggcg gcgctgctgg atgacatcct gtacgaccgg tccggtggca cgggcacgct cgagtacatg gcgccggagc tcctcgagac gaccacctcg cttcgcggca ccaatgagcg gtacgtgaac catcacacga tggcctccga cgtgtggtcg ctggggatgg tgctgcacta cctcgccttc gacggcgcgc tgccggagcg acacgaggac ggcagtgtca acctcgacaa ggcacgccac tcggcgaacg ccagaccgcc tgaaatgctc agactgctgg aggccatgct gcagcttgat cccgcaaagc ggccgcgctg caacgacatc ctcggctccc cgatggtgca gtccatcatg cgcatcttca acaaagatga ccatagccag tgggacctct ccatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat