LemaA.20226.a

Repressor of differentiation kinase 1, putative (RDK1)

CENTER ID: LemaA.20226.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.20226.a.B2.GE42808 PHoranyi kinase domain truncation 1267 1532
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.32.0810
UniProt: Q4Q5K1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNGNSVQPI IIKSKHSCTS SDSSGADSTK SRGALILASA IAVSPGPPNG SSAPTPSRVP EAALASRQPI PSGASPSVTG GGATTGVWYD PTAANFSAQS RGSVIRVLPT AGGYVTGEQT TPAPSWTAPN LTHSASQPGQ HFSSLDLQQS HAINTSTTVD TASTMIRNAT IPAHIQSSPS HSSRDHMSLS LSLQRQKGSN ADDEDYADGR RILMHNPLKG HLFSSSSDSS LHSSIGQPEM IAAASSSVQK GSSDAGNRLD TALGFFGEET AHGLVAEQVE GPCDEKHVNL IEVHAIDSDD DPSLLEEQEP QVCCTIPSLP AVAFIMCFGA IFVGLLGFLP FYFVGVQSNA HTTEYLLSEA MKGVASITQN SLAILPAFVH IVTFNYIKRH NTTLEETNLP KDPDQLLISL LTVLTRFNEA ISYLRFVYEG GLYTNAGYTD PRPTDANQTR RIYGGYSRTH NTVPMLEMYN ESVTVVEPPN VLGYFDFHRE VTLPEGAMNQ IVKTWVADPQ NNTRWLLASD NTTPTYFNFV MPFAVNGSLG FFEAGTSSDT IIEETVTLVS FLRKNGRIFL VDATRNILVG NSWNQSVQSR TNDNAIGATI YTPTRIYEIT EPIILAAMQV VNESGSLTEV LKGKDTALIT FRYKGNNAFL NLTKVRDSYG LDLVLGVVVV RADFEKAFST ARTAAFTVTV LVVVIAAVVA VVTAWFVVRE MKRLIPQLIR ASNLEVSRGD GKDHRVQGWL AYITEIRGIH EAFVRVERSL REMRTFVPAA VMTLAPSDDS HSNADLSDGK TRRARTRFNA NIVKDNTNEF SRVDVAMVLV DVHRAMDTME ACIIMDIISQ RAEEHTGYIE SVSSSSFFVN FGTQSQNPLV VSKICRFALE VYQSVPDFMR NRVVCFAVRM PFLVGTCGAR HSKARVVFDT QRMLDISKVL WDIGCHVAST TDTLSHFATS SAQIPWYRID CVRFPDEVSQ VTLCELRDPC SLLQDDENMP KKMVDGLSKM CKGEYKQALM IFDSAHSENV QLKRLRKICI DRIASGDTSK YIHLIQDVYA LDAAPSNHED MAEGDGAAMY DYDAGSPVGV ATSSGEVCDP SNESPLFACS SFGNGAGMPA PVLSQPAAHA SSPVARHTAP TGHSKTSPTP TPSVTTGGEA AEPSRLTERA VTAAMGPREF PLCGAPQVAG ASGAGPGLPP FHPRTPGTTT TAASVPGSSP PLGARDDNAS EAPSFLTNAF VFDSSMASVA SADAVGATVQ GADEPQVLRD VNQHEWQVSL YPIGSGAFSF IYLGMSDDGV QVAIKRIPRL HRGIKEEEMV SEVCTCAKLR HPNIVPYISC CVTQSYLAII MEYMPGGSLH DIIDNFGKLP RTVVRRFMLD IVNGLAYLHE STTHGDVKPH NILLGVDGVC KLSDFGSASD RLTEACCVNE DRLMRGTAVY ISPEGARNHP LTSASDIYSL GISFLEMVLG RLPWKWADSE RTDALPLRHD HEFVQCLTAN AIVVDIPDDL DNDMRELALA SCAENPEKRP TAQELLSFAF LI
NT Sequence
atgcttaacg gcaatagcgt gcagccgatc atcatcaagt ccaagcactc gtgcacctcc agcgatagca gcggcgcaga cagcactaag tcgcggggcg cactgatttt agccagcgcg atagcggtgt ctccggggcc gccgaacggg tctagtgcgc caacgccgtc tagagtcccc gaggctgcgc tcgcctcccg gcagccgatt ccgtcgggtg ccagcccatc cgtgacaggc ggtggcgcga ccaccggtgt gtggtacgac cccactgccg ccaacttttc agcacaatca cgcggctccg tcattcgcgt tctgccgact gctggtggct acgtcactgg ggagcagacc actcctgcgc cgagctggac cgccccgaat ctgacgcatt ccgcttcgca gccggggcag cacttttcat cgctggatct gcagcaaagt catgcgatta acacgtccac gacagttgac acggcctcga ccatgatacg gaacgccacg atcccggcgc acatccagtc cagcccgagc cacagcagcc gcgaccatat gagcctgtcg ctgagcctgc agaggcagaa gggctcgaac gccgacgatg aggactacgc cgacggccgg cgcatcttga tgcacaaccc gctcaagggc catctcttca gcagcagttc ggactcctcg cttcacagct ccatcggcca gccggagatg atcgcggcgg cgtccagcag tgtgcagaag gggagcagcg acgccggcaa ccgtctcgac acagcgctcg gctttttcgg tgaggagacg gcgcatgggc tggttgctga acaagtcgag ggcccgtgtg atgagaagca cgtgaacctg attgaggtgc acgccatcga ctcggacgac gatccatcct tgcttgagga gcaggagccc caagtttgct gcaccatacc cagtcttccc gccgttgcgt ttatcatgtg ttttggcgct atctttgtgg ggctattggg tttccttccc ttctacttcg tcggcgtcca gtccaacgca cacacaacag agtatctgct gagtgaggcc atgaagggcg tagcctccat cacgcagaac agcctggcga tacttcccgc gtttgttcac attgtgacgt tcaactacat caagcgtcat aacacgacac tggaggagac gaacttgccc aaagacccgg atcagctgct gatatcgctc ctcacggttc tcactcgctt caacgaggcc atctcgtatc tgcggtttgt ctacgaaggc ggtctctaca caaatgccgg ctacacagac ccgaggccaa ccgatgcgaa ccagaccaga aggatctacg gcggttacag tcgcacgcac aacacggtcc ccatgcttga aatgtacaat gagagcgtca ccgtggttga accgcccaac gtattgggtt acttcgactt ccaccgcgaa gtcacgctcc ctgaaggtgc catgaaccag attgtgaaaa cttgggtggc ggacccgcag aacaacaccc gctggctgct ggcctctgat aacaccactc cgacgtactt caacttcgtg atgccttttg ccgtaaacgg ctcccttggc ttcttcgagg ccggcacctc gtcggacacg atcatcgaag aaacagtcac cctcgtaagc tttctgcgca agaacggccg catctttctc gtggatgcca cgcgcaacat tcttgtcggt aacagttgga accaatcagt gcagagtaga acaaatgaca acgcgatcgg cgcaaccatc tacaccccga ctcgcatcta cgaaatcacc gagcccatca ttctcgctgc catgcaggtg gtcaacgaga gcggtagctt gacggaggtg ttgaagggca aggacacggc cctcatcacc tttcggtaca agggcaacaa cgcatttctg aacctcacaa aggtgaggga ctcgtacggg ctggacctcg ttcttggcgt cgtggttgtg cgggctgact tcgagaaggc cttctccacc gcgcgcacag cggccttcac ggtgacggtc ctggtggttg tgattgccgc tgtggtcgcg gtggtcaccg cgtggtttgt cgtgcgggag atgaagcggc tcatcccgca gctcatccgc gcgtcgaacc ttgaggtgtc ccgcggcgac ggcaaggatc accgtgtgca gggctggctg gcgtacatca cggagattcg cggcattcac gaggccttcg tgcgcgtcga gcggagtctg cgagagatgc gcacgtttgt gccggcggcc gtcatgactc tggcgccgtc cgacgactct cacagcaacg cggatctttc tgacggcaag acgcgccgtg cgcgtacccg ctttaacgct aacattgtca aggacaacac gaacgagttc tcacgagtgg atgtggcgat ggtgctggtg gatgtgcacc gcgccatgga cacgatggag gcgtgtatta ttatggatat catctcgcaa cgcgccgaag agcacactgg ctacatcgag tccgtcagct ccagcagttt cttcgtcaac ttcggcaccc agtcacagaa cccgcttgtg gtgtcgaaga tttgccgctt tgcgctcgag gtgtatcagt ccgtgccgga cttcatgcgc aaccgcgtcg tctgcttcgc tgtccgcatg ccgtttctgg tcggcacgtg cggtgcacgg cacagcaagg ctcgcgtggt ctttgacacg cagcggatgc tggacatttc aaaggtgctg tgggacatcg gctgccacgt tgcctccacg acagacacac tctcgcactt cgccacgagc agcgcgcaga tcccgtggta ccggatcgac tgtgtgcgct tcccggacga ggtttcgcag gtgacactgt gcgagctgcg cgacccgtgc tcgttgctgc aggatgatga aaatatgcca aagaagatgg ttgatggtct ctcgaaaatg tgcaagggcg agtacaagca ggcgcttatg atcttcgact cggcgcacag cgagaacgtg cagctcaagc ggctgcgcaa gatctgcatt gaccgcatcg cctctggcga cacaagcaag tacatccatc tcatccagga cgtctacgcg ctggacgccg cccccagcaa ccacgaagac atggcggagg gcgacggtgc tgcgatgtac gactatgacg ccggcagccc cgtcggggtg gcgacgagca gcggcgaggt gtgcgatcca tcaaatgagt caccgttgtt tgcatgcagc tccttcggaa atggtgcggg gatgccggca ccagtgctgt cgcagcccgc agcacacgcc tcgtctcctg tcgcgcgcca caccgcgcca accggccact cgaagacgtc gccgacgccg acgccgtccg tgacgaccgg cggtgaggcg gccgagccct cgcgcctcac cgagcgggcc gtgacggctg ccatgggtcc ccgcgaattc cctctatgcg gagcgccgca ggtggctgga gccagtggcg cgggtcctgg tctccccccc ttccatccac gaacccctgg cactacaacg acggcagcca gcgtgcctgg aagctccccg ccgttgggcg cgcgcgacga caacgcgagc gaggcgccgt cgtttctcac gaacgccttc gtcttcgact cctccatggc ctccgtggcc tccgcggacg ctgttggtgc gactgtgcag ggcgccgacg agccgcaggt gttgcgggac gtgaaccagc atgagtggca ggtctccctc tacccgatcg gcagcggcgc cttcagcttc atttatctgg gcatgtcaga cgatggcgta caagtggcga tcaagcgcat tccccggctg caccgcggca tcaaggagga ggagatggtg tccgaggtat gcacgtgcgc caaactgcga catcccaaca ttgttcccta catctcttgc tgcgtaaccc agtcgtacct ggcaatcatc atggaataca tgccaggggg ctcgctgcac gacatcatcg acaactttgg caagctgcct cgtaccgtgg tgcgccggtt catgctcgac atcgtgaacg gcctagcgta cctgcatgag tccaccacgc acggtgacgt gaagccgcac aacattctgc ttggcgtgga cggcgtctgc aagctctccg acttcggctc tgcctcggac aggctcacgg aggcgtgctg cgtcaacgag gaccgcctga tgcgcggcac ggccgtgtac atctcacccg agggcgcgcg caaccatccc ttgacctccg cctctgacat ctactcgctc ggcatctcgt tcttggagat ggtgcttggc cgcctgccgt ggaagtgggc cgattccgag agaacagacg cgctcccgct gcgccatgac cacgagtttg tgcagtgcct caccgccaat gccattgtcg tcgacattcc cgacgacctg gacaacgaca tgcgcgagtt ggcgctggcg agctgcgctg agaacccgga aaaacgaccg acagcccaag agctgctctc cttcgctttt ctgatttga
Details for LemaA.20226.a.B2.GE42808
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMQ VSLYPIGSGA FSFIYLGMSD DGVQVAIKRI PRLHRGIKEE EMVSEVCTCA KLRHPNIVPY ISCCVTQSYL AIIMEYMPGG SLHDIIDNFG KLPRTVVRRF MLDIVNGLAY LHESTTHGDV KPHNILLGVD GVCKLSDFGS ASDRLTEACC VNEDRLMRGT AVYISPEGAR NHPLTSASDI YSLGISFLEM VLGRLPXXWA DSERTDALXX RXXXEFVQCL XXXAIVXXXX DDXDXXMXXV GXXXXX
Validated NT Sequence
cttgggctgt cggtcnnnnn nccgggttct cacngnnnnn cgcnnnnncc aacnnnnngc atgnnnnngt cnnngtcgtc ngnnnnntnn nnnacaatgg cnttggnnnn naggcactgc acaaactcnn nnnnntggcg nnncnggagc gcgtctgttc tctcggaatc ggcccacnnn nncggcaggc ggccaagcac catctccaag aacgagatgc cgagcgagta gatgtcagag gcggaggtca agggatggtt gcgcgcgccc tcgggtgaga tgtacacggc cgtgccgcgc atcaggcggt cctcgttgac gcagcacgcc tccgtgagcc tgtccgaggc agagccgaag tcggagagct tgcagacgcc gtccacgcca agcagaatgt tgtgcggctt cacgtcaccg tgcgtggtgg actcatgcag gtacgctagg ccgttcacga tgtcgagcat gaaccggcgc accacggtac gaggcagctt gccaaagttg tcgatgatgt cgtgcagcga gccccctggc atgtattcca tgatgattgc caggtacgac tgggttacgc agcaagagat gtagggaaca atgttgggat gtcgcagttt ggcgcacgtg catacctcgg acaccatctc ctcctccttg atgccgcggt gcagccgggg aatgcgcttg atcgccactt gtacgccatc gtctgacatg cccagataaa tgaagctgaa ggcgccgctg ccgatcgggt agagggagac ctgcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHQV SLYPIGSGAF SFIYLGMSDD GVQVAIKRIP RLHRGIKEEE MVSEVCTCAK LRHPNIVPYI SCCVTQSYLA IIMEYMPGGS LHDIIDNFGK LPRTVVRRFM LDIVNGLAYL HESTTHGDVK PHNILLGVDG VCKLSDFGSA SDRLTEACCV NEDRLMRGTA VYISPEGARN HPLTSASDIY SLGISFLEMV LGRLPWKWAD SERTDALPLR HDHEFVQCLT ANAIVVDIPD DLDNDMRELA LASCAENPEK RPTAQELLSF AFLI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaggtct ccctctaccc gatcggcagc ggcgccttca gcttcattta tctgggcatg tcagacgatg gcgtacaagt ggcgatcaag cgcattcccc ggctgcaccg cggcatcaag gaggaggaga tggtgtccga ggtatgcacg tgcgccaaac tgcgacatcc caacattgtt ccctacatct cttgctgcgt aacccagtcg tacctggcaa tcatcatgga atacatgcca gggggctcgc tgcacgacat catcgacaac tttggcaagc tgcctcgtac cgtggtgcgc cggttcatgc tcgacatcgt gaacggccta gcgtacctgc atgagtccac cacgcacggt gacgtgaagc cgcacaacat tctgcttggc gtggacggcg tctgcaagct ctccgacttc ggctctgcct cggacaggct cacggaggcg tgctgcgtca acgaggaccg cctgatgcgc ggcacggccg tgtacatctc acccgagggc gcgcgcaacc atcccttgac ctccgcctct gacatctact cgctcggcat ctcgttcttg gagatggtgc ttggccgcct gccgtggaag tgggccgatt ccgagagaac agacgcgctc ccgctgcgcc atgaccacga gtttgtgcag tgcctcaccg ccaatgccat tgtcgtcgac attcccgacg acctggacaa cgacatgcgc gagttggcgc tggcgagctg cgctgagaac ccggaaaaac gaccgacagc ccaagagctg ctctccttcg cttttctgat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat