SSGCID: Target Page

LemaA.20203.a

tyrosyl-tRNA synthetase, putative

CENTER ID:	LemaA.20203.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.20203.a.B1.GE42546	full length		1	682

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.20203.a.B1.PW38517	full length		1	682

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.14.1370
UniProt:	Q4QFJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTDERYKLL RSVGEECIQE SELRNLIEKK PLIRCYDGFE PSGRMHIAQG IFKAVNVNKC TAAGCEFVFW VADWFALMND KVGGELEKIR IVGRYLIEVW KAAGMDMDKV LFLWSSEEIT SHADTYWRMV LDIGRQNTIA RIKKCCTIMG KTEGTLTAAQ VLYPLMQCCD IFFLKADICQ LGLDQRKVNM LAREYCDLIG RKLKPVILSH HMLAGLKQGQ AKMSKSDPDS AIFMEDTEED VARKIRQAYC PRVKQSASAI TDDGAPVATD DRNPVLDYFQ CVVYARPGAA ATIDGTTYAT YEDLEQAFVS DEVSEDALKS CLIDEVNALL EPVRQHFASN EEAHELLEAV KSYRKDGATL PLAETALPAA PAKPHACMWM PALLKVPLDV AEGMIKVTKD FIAAHPEGTV TLVLPDWSAV ASDEITGVEK DISAALQVNC ALLKAYGLPS SVKIVTENEV ILGNCDDFWV SVIGIARKNL LSHVEELYGG EVRNAGQVIA ALMRVATALM LSVSHVISTS LDGHINAFAR EYTKERIDCV QTLEGRIPAL HRPGAAPAVL GADDVLYLDD NDMDIRRKIK KAYSAPNEEA NPVISVAQHL LAQHGALSIE RGEANGGNVS YNTPEALVAD CGSGALHPAD LKAAVLQLLL DRSAQARALL NGELKKNMTV LRNAEKKMAK KR

NT Sequence

atgaacacgg acgagcgcta caagctcctc cgcagcgtcg gagaggagtg cattcaggaa agtgagctgc gcaaccttat cgaaaagaag ccgctcattc gctgctacga tggcttcgag ccctccggcc gcatgcacat tgctcagggt atcttcaagg cagtcaatgt caacaagtgc accgccgccg ggtgcgagtt tgtcttctgg gtggcggact ggtttgcgct catgaacgac aaggtcggcg gcgaactgga gaagatccgc attgttggca ggtaccttat tgaggtgtgg aaggcggcgg gcatggacat ggacaaggtt ttgtttttat ggagcagcga ggagatcacg agccacgccg acacgtactg gcgcatggtg ctcgacattg gccgccagaa tacgatcgcc cgcatcaaga agtgctgcac catcatgggc aagacggaag gcacgctgac ggcggcacag gtgctgtacc cgctgatgca gtgctgcgac atcttctttc tcaaggccga catctgccag ctgggcctgg accagcgcaa ggtgaacatg ctcgcacgcg agtactgcga tctgatcggc cgcaagttga agccggtcat tctgtcacac cacatgctgg ccggtctgaa gcaggggcag gcgaagatga gcaagagtga cccggacagt gccatcttca tggaagacac cgaggaggat gtggcacgta agatccgtca ggcctactgt ccgcgcgtca agcagtccgc cagcgccatc acagatgacg gggcgccggt cgccacggac gaccgcaacc cggtgctgga ctacttccag tgtgtcgtct acgcccgacc cggcgctgcg gccaccattg acggcaccac ctacgcgacc tacgaggacc tcgagcaggc ttttgtgagc gacgaggtca gcgaggatgc cctcaagtcg tgcctgattg atgaggtgaa cgccctgctg gagcccgtgc gccaacattt tgcgtcaaac gaggaggctc acgagctgct ggaggcggtg aagtcgtacc gtaaagatgg cgcgaccctt ccactggccg agacagcgct ccctgccgcc cccgcaaagc cgcacgcgtg catgtggatg ccggcgctgc tgaaggtgcc gctggatgtc gcggaaggga tgatcaaagt caccaaggac ttcatcgccg cccacccgga gggcacggtg acactggtgc tgcctgactg gtcggcggtg gccagcgacg aaatcaccgg ggtggagaaa gacatctcgg ccgccctcca ggtgaactgt gcgcttctca aggcgtatgg actgccgagc tcggtgaaga tcgtgacgga gaacgaggtc attctcggca actgcgacga cttctgggtg agtgtaatcg gcattgcacg caagaacctg ctgagccacg tcgaggagct gtacggggga gaggtacgca acgctggtca ggtcattgct gccctcatgc gcgttgccac ggctctgatg ctatccgtct cgcacgtcat ctcaacctcg ctggatggac acatcaacgc ctttgcccgc gagtacacga aggagcggat tgactgcgtg cagacgctgg agggacgcat cccggcgctg caccgccctg gcgccgcccc agcggtgctc ggtgccgacg acgtcctata cctggacgac aatgacatgg acatccgccg taaaattaag aaggcgtact cggcgccgaa cgaggaggcc aatccggtca tatcggttgc ccagcacctt ctcgcacagc atggagccct cagcatcgag cgcggggagg ccaacggtgg caacgtaagc tacaacacgc cagaggccct cgtggcagac tgcggcagcg gcgctctcca ccctgccgac ctcaaggcgg cggtgctaca gctgctgctg gaccggtcgg cgcaggcgcg cgcgctgctc aacggcgagc tcaagaagaa tatgaccgtt cttcgcaacg cggagaagaa gatggcaaag aagaggtaa

Details for LemaA.20203.a.B1.GE42546

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	76kDa
OBSERVED MW:	76kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN TDERYKLLRS VGEECIQESE LRNLIEKKPL IRCYDGFEPS GRMHIAQGIF KAVNVNKCTA AGCEFVFWVA DWFALMNDKV GGELEKIRIV GRYLIEVWKA AGMDMDKVLF LWSSEEITSH ADTYWRMVLD IGRQNTIARI KKCCTIMGKT EGTLTAAQVL YPLMQCCDIF FLKADICQLG LDQRKVNMLA REYCDLIGRK LKPVILSHHM LAGLKQGQAK MSKSDPDSAI FMEDTEEDVA RKIRQAYCPR VKQSASAITD DGAPVATDDR NPVLDYFQCV VYARPGAAAT IDGTTYATYE DLEQAFVSDE VSEDALKSCL IDEVNALLEP VRQHFASNEE AHELLEAVKS YRKDGATLPL AETALPAAPA KPHACMWMPA LLKVPLDVAE GMIKVTKDFI AAHPEGTVTL VLPDWSAVAS DEITGVEKDI SAALQVNCAL LKAYGLPSSV KIVTENEVIL GNCDDFWVSV IGIARKNLLS HVEELYGGEV RNAGQVIAAL MRVATALMLS VSHVISTSLD GHINAFAREY TKERIDCVQT LEGRIPALHR PGAAPAVLGA DDVLYLDDND MDIRRKIKKA YSAPNEEANP VISVAQHLLA QHGALSIERG EANGGNVSYN TPEALVADCG SGALHPADLK AAVLQLLLDR SAQARALLNG ELKKNMTVLR NAEKKMAKKR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca cggacgagcg ctacaagctc ctccgcagcg tcggagagga gtgcattcag gaaagtgagc tgcgcaacct tatcgaaaag aagccgctca ttcgctgcta cgatggcttc gagccctccg gccgcatgca cattgctcag ggtatcttca aggcagtcaa tgtcaacaag tgcaccgccg ccgggtgcga gtttgtcttc tgggtggcgg actggtttgc gctcatgaac gacaaggtcg gcggcgaact ggagaagatc cgcattgttg gcaggtacct tattgaggtg tggaaggcgg cgggcatgga catggacaag gttttgtttt tatggagcag cgaggagatc acgagccacg ccgacacgta ctggcgcatg gtgctcgaca ttggccgcca gaatacgatc gcccgcatca agaagtgctg caccatcatg ggcaagacgg aaggcacgct gacggcggca caggtgctgt acccgctgat gcagtgctgc gacatcttct ttctcaaggc cgacatctgc cagctgggcc tggaccagcg caaggtgaac atgctcgcac gcgagtactg cgatctgatc ggccgcaagt tgaagccggt cattctgtca caccacatgc tggccggtct gaagcagggg caggcgaaga tgagcaagag tgacccggac agtgccatct tcatggaaga caccgaggag gatgtggcac gtaagatccg tcaggcctac tgtccgcgcg tcaagcagtc cgccagcgcc atcacagatg acggggcgcc ggtcgccacg gacgaccgca acccggtgct ggactacttc cagtgtgtcg tctacgcccg acccggcgct gcggccacca ttgacggcac cacctacgcg acctacgagg acctcgagca ggcttttgtg agcgacgagg tcagcgagga tgccctcaag tcgtgcctga ttgatgaggt gaacgccctg ctggagcccg tgcgccaaca ttttgcgtca aacgaggagg ctcacgagct gctggaggcg gtgaagtcgt accgtaaaga tggcgcgacc cttccactgg ccgagacagc gctccctgcc gcccccgcaa agccgcacgc gtgcatgtgg atgccggcgc tgctgaaggt gccgctggat gtcgcggaag ggatgatcaa agtcaccaag gacttcatcg ccgcccaccc ggagggcacg gtgacactgg tgctgcctga ctggtcggcg gtggccagcg acgaaatcac cggggtggag aaagacatct cggccgccct ccaggtgaac tgtgcgcttc tcaaggcgta tggactgccg agctcggtga agatcgtgac ggagaacgag gtcattctcg gcaactgcga cgacttctgg gtgagtgtaa tcggcattgc acgcaagaac ctgctgagcc acgtcgagga gctgtacggg ggagaggtac gcaacgctgg tcaggtcatt gctgccctca tgcgcgttgc cacggctctg atgctatccg tctcgcacgt catctcaacc tcgctggatg gacacatcaa cgcctttgcc cgcgagtaca cgaaggagcg gattgactgc gtgcagacgc tggagggacg catcccggcg ctgcaccgcc ctggcgccgc cccagcggtg ctcggtgccg acgacgtcct atacctggac gacaatgaca tggacatccg ccgtaaaatt aagaaggcgt actcggcgcc gaacgaggag gccaatccgg tcatatcggt tgcccagcac cttctcgcac agcatggagc cctcagcatc gagcgcgggg aggccaacgg tggcaacgta agctacaaca cgccagaggc cctcgtggca gactgcggca gcggcgctct ccaccctgcc gacctcaagg cggcggtgct acagctgctg ctggaccggt cggcgcaggc gcgcgcgctg ctcaacggcg agctcaagaa gaatatgacc gttcttcgca acgcggagaa gaagatggca aagaagaggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for LemaA.20203.a.B1.PW38517

PURIFICATION DATe:	7/30/2018
CONCENTRATION:	15.19mg/ml
OBSERVED MW:	76kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM Tyrosine
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA CGGACGAGCG CTACAAGCTC CTCCGCAGCG TCGGAGAGGA GTGCATTCAG GAAAGTGAGC TGCGCAACCT TATCGAAAAG AAGCCGCTCA TTCGCTGCTA CGATGGCTTC GAGCCCTCCG GCCGCATGCA CATTGCTCAG GGTATCTTCA AGGCAGTCAA TGTCAACAAG TGCACCGCCG CCGGGTGCGA GTTTGTCTTC TGGGTGGCGG ACTGGTTTGC GCTCATGAAC GACAAGGTCG GCGGCGAACT GGAGAAGATC CGCATTGTTG GCAGGTACCT TATTGAGGTG TGGAAGGCGG CGGGCATGGA CATGGACAAG GTTTTGTTTT TATGGAGCAG CGAGGAGATC ACGAGCCACG CCGACACGTA CTGGCGCATG GTGCTCGACA TTGGCCGCCA GAATACGATC GCCCGCATCA AGAAGTGCTG CACCATCATG GGCAAGACGG AAGGCACGCT GACGGCGGCA CAGGTGCTGT ACCCGCTGAT GCAGTGCTGC GACATCTTCT TTCTCAAGGC CGACATCTGC CAGCTGGGCC TGGACCAGCG CAAGGTGAAC ATGCTCGCAC GCGAGTACTG CGATCTGATC GGCCGCAAGT TGAAGCCGGT CATTCTGTCA CACCACATGC TGGCCGGTCT GAAGCAGGGG CAGGCGAAGA TGAGCAAGAG TGACCCGGAC AGTGCCATCT TCATGGAAGA CACCGAGGAG GATGTGGCAC GTAAGATCCG TCAGGCCTAC TGTCCGCGCG TCAAGCAGTC CGCCAGCGCC ATCACAGATG ACGGGGCGCC GGTCGCCACG GACGACCGCA ACCCGGTGCT GGACTACTTC CAGTGTGTCG TCTACGCCCG ACCCGGCGCT GCGGCCACCA TTGACGGCAC CACCTACGCG ACCTACGAGG ACCTCGAGCA GGCTTTTGTG AGCGACGAGG TCAGCGAGGA TGCCCTCAAG TCGTGCCTGA TTGATGAGGT GAACGCCCTG CTGGAGCCCG TGCGCCAACA TTTTGCGTCA AACGAGGAGG CTCACGAGCT GCTGGAGGCG GTGAAGTCGT ACCGTAAAGA TGGCGCGACC CTTCCACTGG CCGAGACAGC GCTCCCTGCC GCCCCCGCAA AGCCGCACGC GTGCATGTGG ATGCCGGCGC TGCTGAAGGT GCCGCTGGAT GTCGCGGAAG GGATGATCAA AGTCACCAAG GACTTCATCG CCGCCCACCC GGAGGGCACG GTGACACTGG TGCTGCCTGA CTGGTCGGCG GTGGCCAGCG ACGAAATCAC CGGGGTGGAG AAAGACATCT CGGCCGCCCT CCAGGTGAAC TGTGCGCTTC TCAAGGCGTA TGGACTGCCG AGCTCGGTGA AGATCGTGAC GGAGAACGAG GTCATTCTCG GCAACTGCGA CGACTTCTGG GTGAGTGTAA TCGGCATTGC ACGCAAGAAC CTGCTGAGCC ACGTCGAGGA GCTGTACGGG GGAGAGGTAC GCAACGCTGG TCAGGTCATT GCTGCCCTCA TGCGCGTTGC CACGGCTCTG ATGCTATCCG TCTCGCACGT CATCTCAACC TCGCTGGATG GACACATCAA CGCCTTTGCC CGCGAGTACA CGAAGGAGCG GATTGACTGC GTGCAGACGC TGGAGGGACG CATCCCGGCG CTGCACCGCC CTGGCGCCGC CCCAGCGGTG CTCGGTGCCG ACGACGTCCT ATACCTGGAC GACAATGACA TGGACATCCG CCGTAAAATT AAGAAGGCGT ACTCGGCGCC GAACGAGGAG GCCAATCCGG TCATATCGGT TGCCCAGCAC CTTCTCGCAC AGCATGGAGC CCTCAGCATC GAGCGCGGGG AGGCCAACGG TGGCAACGTA AGCTACAACA CGCCAGAGGC CCTCGTGGCA GACTGCGGCA GCGGCGCTCT CCACCCTGCC GACCTCAAGG CGGCGGTGCT ACAGCTGCTG CTGGACCGGT CGGCGCAGGC GCGCGCGCTG CTCAACGGCG AGCTCAAGAA GAATATGACC GTTCTTCGCA ACGCGGAGAA GAAGATGGCA AAGAAGAGGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT