LemaA.20202.a

hypothetical protein, conserved

CENTER ID: LemaA.20202.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.20202.a.B1.GE42545 full length 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.20202.a.B1.PW38583 full length 1 491

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.15.0690
UniProt: Q4QFB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVFMRVGE LACQKDSFLR ELTATVVSCE PAKTAVPAAK KAKGASNDTV AYDVVLSDSV LFPEGGGQPC DHGTLRVVSG DNDKADPSCD ATAAPPLSIT NVRRVGDTCI LTSPAALPVG ATVHQMVDWP RRMDHMQHHT GQHLLTAVVE RKDTLRLPTV SWSLTHPYCF IQVDVGGYAS DPASPYHAYI TKEKKISDEM VAKIEALCND AIAHSTTVQC DVFENREVYM REQDRRHQAA AVSADGGAEA FRSRGIPEDV TGPIRIISLD AIDSCTCCGT HLHTLAELQA MYLLHQEVKG TLVKLYFITG ERAAQQFHAM YQRERQLMPE LGGSRPDDFV SVVQRRSKDA TDLEKLMKRW TLELASVEAQ RIAAAVPASH AATVMVPLRR DDVDLEFFNE VRARLEAAGL RRVVLVSAWA AERCTAGTLA SAKDVAGQVF VTGGETPADM EKAVQLVKET LQGMKGGTSK LGFRGKGSLK EWDTLVARLK E
NT Sequence
atgacggccg tattcatgcg cgtcggtgag ctggcgtgtc agaaagactc gttcctgcga gagttgaccg cgactgttgt ctcgtgtgag ccggccaaga cggcagtgcc cgcggcgaag aaggcgaagg gtgccagcaa cgacaccgtg gcgtacgatg ttgttctgag cgactccgtc ctttttccag aaggtggtgg tcagccctgc gaccacggga cactgcgagt ggtgtcaggt gataatgaca aagccgatcc ctcgtgcgac gccaccgccg cgccgccgct ctccattacg aacgtgcgcc gggtcgggga cacatgcata ctcaccagtc ccgccgccct ccccgtgggg gctacggtgc accagatggt ggactggccg cgtcggatgg accacatgca gcaccacacc ggccagcacc tgctcactgc cgtggtagaa cgcaaggaca cgctgcgctt gcccaccgtg agctggtccc tgacgcaccc ttactgtttc atccaagtgg acgttggcgg ctacgcaagc gacccagcct cgccgtatca cgcttacatc accaaggaga agaaaatatc ggacgaaatg gtggcgaaga tcgaggcgct ctgcaacgac gcgatcgccc acagcaccac cgtgcagtgc gacgtgttcg aaaatcgaga ggtgtatatg cgggagcagg atcgccggca tcaggcggct gcggtctcgg cagacggcgg cgcggaggcc ttccgaagcc gtggcatccc agaagacgtc acaggaccga ttcgcatcat ctcgctggac gccatcgaca gctgcacctg ctgcggcacc cacctgcaca cgctggcaga gctgcaggcc atgtacctcc tccaccaaga ggtgaaagga acgctagtga agctgtactt catcactggc gagcgggcag cgcagcagtt ccacgccatg tatcagcggg agcggcaatt gatgccggag ttgggtggca gcagacccga cgactttgtc tccgtggtgc agcgccgttc caaagatgcg acggaccttg agaagctgat gaagcggtgg acactggagc tagcgagcgt agaagcacag cgcatcgccg ccgctgtgcc agcgtcgcac gccgctaccg tcatggtacc cctgcgtcga gacgacgtag atctcgagtt cttcaacgag gtccgcgctc ggctcgaagc agcagggcta cgccgtgtcg ttctcgtttc cgcgtgggcg gcagagcggt gtacggcagg cacgctcgcc agcgcgaagg atgtggcggg ccaggtcttc gtcaccggcg gggagacgcc tgcggacatg gagaaggccg tccagctggt gaaggaaacg ctacagggga tgaagggggg cacctccaag ctaggtttcc gtgggaaggg cagtctgaag gagtgggaca ccctcgtggc gcgtctgaag gagtag
Details for LemaA.20202.a.B1.GE42545
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVPAAKKA KGASNDTVAY DVVLSDSVLF PEGGGQPCDH GTLRVVSGDN DKADPSCDAT AAPPLSITNV RRVGDTCILT SPAALPVGAT VHQMVDWPRR MDHMQHHTGQ HLLTAVVERK DTLRLPTVSW SLTHPYCFIQ VDVGGYASDP ASPYHAYITK EKKISDEMVA KIEALCNDAI AHSTTVQCDV FENREVYMRE QDRRHQAAAV SADGGAEAFR SRGIPEDVTG PIRIISLDAI DSCTCCGTHL HTLAELQAMY LLHQEVKGTL VKLYFITGER AAQQFHAMYQ RERQLMPELG GSRPDDFVSV VQRRSKDATD LEKLMKRWTL ELASVEAQRI AAAVPASHAA TVMVPLRRDD VDLEFFNEVR ARLEAAGLRR VVLVSAWAAE RCTAGTLASA KDVAGQVFVT GGETPADMEK AVQLVKETLQ GMKGGTSKLG FRGKGSLKEW D
Validated NT Sequence
gatataccat ggctcaccac caccaccacc atatgacggc cgtattcatg cgcgtcggtg agctggcgtg tcagaaagac tcgttcctgc gagagttgac cgcgactgtt gtctcgtgtg agccggccaa gacggcagtg cccgcggcga agaaggcgaa gggtgccagc aacgacaccg tggcgtacga tgttgttctg agcgactccg tcctttttcc agaaggtggt ggtcagccct gcgaccacgg gacactgcga gtggtgtcag gtgataatga caaagccgat ccctcgtgcg acgccaccgc cgcgccgccg ctctccatta cgaacgtgcg ccgggtcggg gacacatgca tactcaccag tcccgccgcc ctccccgtgg gggctacggt gcaccagatg gtggactggc cgcgtcggat ggaccacatg cagcaccaca ccggccagca cctgctcact gccgtggtag aacgcaagga cacgctgcgc ttgcccaccg tgagctggtc cctgacgcac ccttactgtt tcatccaagt ggacgttggc ggctacgcaa gcgacccagc ctcgccgtat cacgcttaca tcaccaagga gaagaaaata tcggacgaaa tggtggcgaa gatcgaggcg ctctgcaacg acgcgatcgc ccacagcacc accgtgcagt gcgacgtgtt cgaaaatcga gaggtgtata tgcgggagca ggatcgccgg catcaggcgg ctgcggtctc ggcagacggc ggcgcggagg ccttccgaag ccgtggcatc ccagaagacg tcacaggacc gattcgcatc atctcgctgg acgccatcga cagctgcacc tgctgcggca cccacctgca cacgctggca gagctgcagg ccatgtacct cctccaccaa gaggtgaaag gaacgctagt gaagctgtac ttcatcactg gcgagcgggc agcgcagcag ttccacgcca tgtatcagcg ggagcggcaa ttgatgccgg agttgggtgg cagcagaccc gacgactttg tctccgtggt gcagcgccgt tccaaagatg cgacggacct tgagaagctg atgaagcggt ggacactgga gctagcgagc gtagaagcac agcgcatcgc cgccgctgtg ccagcgtcgc acgccgctac cgtcatggta cccctgcgtc gagacgacgt agatctcgag ttcttcaacg aggtccgcgc tcggctcgaa gcagcagggc tacgccgtgt cgttctcgtt tccgcgtggg cggcagagcg gtgtacggca ggcacgctcg ccagcgcgaa ggatgtggcg ggccaggtct tcgtcaccgg cggggagacg cctgcggaca tggagaaggc cgtccagctg gtgaaggaaa cgctacaggg gatgaagggg ggcacctcca agctaggttt ccgtgggaag ggcagtctga aggagtggga cac
Expected Protein Sequence
MAHHHHHHMT AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVPAAKKA KGASNDTVAY DVVLSDSVLF PEGGGQPCDH GTLRVVSGDN DKADPSCDAT AAPPLSITNV RRVGDTCILT SPAALPVGAT VHQMVDWPRR MDHMQHHTGQ HLLTAVVERK DTLRLPTVSW SLTHPYCFIQ VDVGGYASDP ASPYHAYITK EKKISDEMVA KIEALCNDAI AHSTTVQCDV FENREVYMRE QDRRHQAAAV SADGGAEAFR SRGIPEDVTG PIRIISLDAI DSCTCCGTHL HTLAELQAMY LLHQEVKGTL VKLYFITGER AAQQFHAMYQ RERQLMPELG GSRPDDFVSV VQRRSKDATD LEKLMKRWTL ELASVEAQRI AAAVPASHAA TVMVPLRRDD VDLEFFNEVR ARLEAAGLRR VVLVSAWAAE RCTAGTLASA KDVAGQVFVT GGETPADMEK AVQLVKETLQ GMKGGTSKLG FRGKGSLKEW DTLVARLKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ccgtattcat gcgcgtcggt gagctggcgt gtcagaaaga ctcgttcctg cgagagttga ccgcgactgt tgtctcgtgt gagccggcca agacggcagt gcccgcggcg aagaaggcga agggtgccag caacgacacc gtggcgtacg atgttgttct gagcgactcc gtcctttttc cagaaggtgg tggtcagccc tgcgaccacg ggacactgcg agtggtgtca ggtgataatg acaaagccga tccctcgtgc gacgccaccg ccgcgccgcc gctctccatt acgaacgtgc gccgggtcgg ggacacatgc atactcacca gtcccgccgc cctccccgtg ggggctacgg tgcaccagat ggtggactgg ccgcgtcgga tggaccacat gcagcaccac accggccagc acctgctcac tgccgtggta gaacgcaagg acacgctgcg cttgcccacc gtgagctggt ccctgacgca cccttactgt ttcatccaag tggacgttgg cggctacgca agcgacccag cctcgccgta tcacgcttac atcaccaagg agaagaaaat atcggacgaa atggtggcga agatcgaggc gctctgcaac gacgcgatcg cccacagcac caccgtgcag tgcgacgtgt tcgaaaatcg agaggtgtat atgcgggagc aggatcgccg gcatcaggcg gctgcggtct cggcagacgg cggcgcggag gccttccgaa gccgtggcat cccagaagac gtcacaggac cgattcgcat catctcgctg gacgccatcg acagctgcac ctgctgcggc acccacctgc acacgctggc agagctgcag gccatgtacc tcctccacca agaggtgaaa ggaacgctag tgaagctgta cttcatcact ggcgagcggg cagcgcagca gttccacgcc atgtatcagc gggagcggca attgatgccg gagttgggtg gcagcagacc cgacgacttt gtctccgtgg tgcagcgccg ttccaaagat gcgacggacc ttgagaagct gatgaagcgg tggacactgg agctagcgag cgtagaagca cagcgcatcg ccgccgctgt gccagcgtcg cacgccgcta ccgtcatggt acccctgcgt cgagacgacg tagatctcga gttcttcaac gaggtccgcg ctcggctcga agcagcaggg ctacgccgtg tcgttctcgt ttccgcgtgg gcggcagagc ggtgtacggc aggcacgctc gccagcgcga aggatgtggc gggccaggtc ttcgtcaccg gcggggagac gcctgcggac atggagaagg ccgtccagct ggtgaaggaa acgctacagg ggatgaaggg gggcacctcc aagctaggtt tccgtgggaa gggcagtctg aaggagtggg acaccctcgt ggcgcgtctg aaggagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for LemaA.20202.a.B1.PW38583
PURIFICATION DATe: 12/24/2018
CONCENTRATION: 19.83mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 8, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVPAAKKA KGASNDTVAY DVVLSDSVLF PEGGGQPCDH GTLRVVSGDN DKADPSCDAT AAPPLSITNV RRVGDTCILT SPAALPVGAT VHQMVDWPRR MDHMQHHTGQ HLLTAVVERK DTLRLPTVSW SLTHPYCFIQ VDVGGYASDP ASPYHAYITK EKKISDEMVA KIEALCNDAI AHSTTVQCDV FENREVYMRE QDRRHQAAAV SADGGAEAFR SRGIPEDVTG PIRIISLDAI DSCTCCGTHL HTLAELQAMY LLHQEVKGTL VKLYFITGER AAQQFHAMYQ RERQLMPELG GSRPDDFVSV VQRRSKDATD LEKLMKRWTL ELASVEAQRI AAAVPASHAA TVMVPLRRDD VDLEFFNEVR ARLEAAGLRR VVLVSAWAAE RCTAGTLASA KDVAGQVFVT GGETPADMEK AVQLVKETLQ GMKGGTSKLG FRGKGSLKEW D
Validated NT Sequence
gatataccat ggctcaccac caccaccacc atatgacggc cgtattcatg cgcgtcggtg agctggcgtg tcagaaagac tcgttcctgc gagagttgac cgcgactgtt gtctcgtgtg agccggccaa gacggcagtg cccgcggcga agaaggcgaa gggtgccagc aacgacaccg tggcgtacga tgttgttctg agcgactccg tcctttttcc agaaggtggt ggtcagccct gcgaccacgg gacactgcga gtggtgtcag gtgataatga caaagccgat ccctcgtgcg acgccaccgc cgcgccgccg ctctccatta cgaacgtgcg ccgggtcggg gacacatgca tactcaccag tcccgccgcc ctccccgtgg gggctacggt gcaccagatg gtggactggc cgcgtcggat ggaccacatg cagcaccaca ccggccagca cctgctcact gccgtggtag aacgcaagga cacgctgcgc ttgcccaccg tgagctggtc cctgacgcac ccttactgtt tcatccaagt ggacgttggc ggctacgcaa gcgacccagc ctcgccgtat cacgcttaca tcaccaagga gaagaaaata tcggacgaaa tggtggcgaa gatcgaggcg ctctgcaacg acgcgatcgc ccacagcacc accgtgcagt gcgacgtgtt cgaaaatcga gaggtgtata tgcgggagca ggatcgccgg catcaggcgg ctgcggtctc ggcagacggc ggcgcggagg ccttccgaag ccgtggcatc ccagaagacg tcacaggacc gattcgcatc atctcgctgg acgccatcga cagctgcacc tgctgcggca cccacctgca cacgctggca gagctgcagg ccatgtacct cctccaccaa gaggtgaaag gaacgctagt gaagctgtac ttcatcactg gcgagcgggc agcgcagcag ttccacgcca tgtatcagcg ggagcggcaa ttgatgccgg agttgggtgg cagcagaccc gacgactttg tctccgtggt gcagcgccgt tccaaagatg cgacggacct tgagaagctg atgaagcggt ggacactgga gctagcgagc gtagaagcac agcgcatcgc cgccgctgtg ccagcgtcgc acgccgctac cgtcatggta cccctgcgtc gagacgacgt agatctcgag ttcttcaacg aggtccgcgc tcggctcgaa gcagcagggc tacgccgtgt cgttctcgtt tccgcgtggg cggcagagcg gtgtacggca ggcacgctcg ccagcgcgaa ggatgtggcg ggccaggtct tcgtcaccgg cggggagacg cctgcggaca tggagaaggc cgtccagctg gtgaaggaaa cgctacaggg gatgaagggg ggcacctcca agctaggttt ccgtgggaag ggcagtctga aggagtggga cac
Expressed Protein Sequence
MAHHHHHHMT AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVPAAKKA KGASNDTVAY DVVLSDSVLF PEGGGQPCDH GTLRVVSGDN DKADPSCDAT AAPPLSITNV RRVGDTCILT SPAALPVGAT VHQMVDWPRR MDHMQHHTGQ HLLTAVVERK DTLRLPTVSW SLTHPYCFIQ VDVGGYASDP ASPYHAYITK EKKISDEMVA KIEALCNDAI AHSTTVQCDV FENREVYMRE QDRRHQAAAV SADGGAEAFR SRGIPEDVTG PIRIISLDAI DSCTCCGTHL HTLAELQAMY LLHQEVKGTL VKLYFITGER AAQQFHAMYQ RERQLMPELG GSRPDDFVSV VQRRSKDATD LEKLMKRWTL ELASVEAQRI AAAVPASHAA TVMVPLRRDD VDLEFFNEVR ARLEAAGLRR VVLVSAWAAE RCTAGTLASA KDVAGQVFVT GGETPADMEK AVQLVKETLQ GMKGGTSKLG FRGKGSLKEW DTLVARLKE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGG CCGTATTCAT GCGCGTCGGT GAGCTGGCGT GTCAGAAAGA CTCGTTCCTG CGAGAGTTGA CCGCGACTGT TGTCTCGTGT GAGCCGGCCA AGACGGCAGT GCCCGCGGCG AAGAAGGCGA AGGGTGCCAG CAACGACACC GTGGCGTACG ATGTTGTTCT GAGCGACTCC GTCCTTTTTC CAGAAGGTGG TGGTCAGCCC TGCGACCACG GGACACTGCG AGTGGTGTCA GGTGATAATG ACAAAGCCGA TCCCTCGTGC GACGCCACCG CCGCGCCGCC GCTCTCCATT ACGAACGTGC GCCGGGTCGG GGACACATGC ATACTCACCA GTCCCGCCGC CCTCCCCGTG GGGGCTACGG TGCACCAGAT GGTGGACTGG CCGCGTCGGA TGGACCACAT GCAGCACCAC ACCGGCCAGC ACCTGCTCAC TGCCGTGGTA GAACGCAAGG ACACGCTGCG CTTGCCCACC GTGAGCTGGT CCCTGACGCA CCCTTACTGT TTCATCCAAG TGGACGTTGG CGGCTACGCA AGCGACCCAG CCTCGCCGTA TCACGCTTAC ATCACCAAGG AGAAGAAAAT ATCGGACGAA ATGGTGGCGA AGATCGAGGC GCTCTGCAAC GACGCGATCG CCCACAGCAC CACCGTGCAG TGCGACGTGT TCGAAAATCG AGAGGTGTAT ATGCGGGAGC AGGATCGCCG GCATCAGGCG GCTGCGGTCT CGGCAGACGG CGGCGCGGAG GCCTTCCGAA GCCGTGGCAT CCCAGAAGAC GTCACAGGAC CGATTCGCAT CATCTCGCTG GACGCCATCG ACAGCTGCAC CTGCTGCGGC ACCCACCTGC ACACGCTGGC AGAGCTGCAG GCCATGTACC TCCTCCACCA AGAGGTGAAA GGAACGCTAG TGAAGCTGTA CTTCATCACT GGCGAGCGGG CAGCGCAGCA GTTCCACGCC ATGTATCAGC GGGAGCGGCA ATTGATGCCG GAGTTGGGTG GCAGCAGACC CGACGACTTT GTCTCCGTGG TGCAGCGCCG TTCCAAAGAT GCGACGGACC TTGAGAAGCT GATGAAGCGG TGGACACTGG AGCTAGCGAG CGTAGAAGCA CAGCGCATCG CCGCCGCTGT GCCAGCGTCG CACGCCGCTA CCGTCATGGT ACCCCTGCGT CGAGACGACG TAGATCTCGA GTTCTTCAAC GAGGTCCGCG CTCGGCTCGA AGCAGCAGGG CTACGCCGTG TCGTTCTCGT TTCCGCGTGG GCGGCAGAGC GGTGTACGGC AGGCACGCTC GCCAGCGCGA AGGATGTGGC GGGCCAGGTC TTCGTCACCG GCGGGGAGAC GCCTGCGGAC ATGGAGAAGG CCGTCCAGCT GGTGAAGGAA ACGCTACAGG GGATGAAGGG GGGCACCTCC AAGCTAGGTT TCCGTGGGAA GGGCAGTCTG AAGGAGTGGG ACACCCTCGT GGCGCGTCTG AAGGAGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T