SSGCID: Target Page

LemaA.20196.a

PNUTS

CENTER ID:	LemaA.20196.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.20196.a.B1.GE42328	full length		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.33.1320
UniProt:	Q4Q456

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVEEPQPSS PVAVNDDAED VVEAELVAYD AHHTHLVEGD FFPAEAPEVE ADGAVADATE EVDALVDAIA AEAAAVAAVS VVSTPPKPAE VPSRKRVCWA DEGHPDTARG LVKHVTNFYM PNTFSRGSRY DAADQSSPTR QTNATAVNPT PPLFREKVNS DATTYKFCKA QRPALQYAEW RRQQHEYGVT PFELQPVEAG ELTSSASVAT RTLFGELFQR SVVARVAEGQ VRIRNGTHHN SQDNGFQQPF VRIDATFDEP LPIPL

NT Sequence

atgagcgtcg aggagccgca gccaagttcg ccggttgcag tgaacgatga cgccgaggac gtggtggaag cggagttggt cgcttacgac gcgcatcaca cgcaccttgt cgaaggcgac tttttcccgg ccgaagcgcc ggaggtggag gctgatggag cagtcgctga cgctactgag gaagtcgacg cgctcgttga cgccattgcc gccgaagcag cggcagtggc cgcagtgtcg gtggtgtcga ccccgccaaa gccggccgaa gtgccgtccc gcaagcgggt ttgctgggcc gatgaaggcc accccgacac cgcccgcggc ttggtgaagc acgtcacgaa cttctacatg cccaacacct tctcacgcgg ctcccgttac gacgccgccg accaatctag ccctacgcgg cagaccaacg cgacggccgt caacccgacc ccacccctgt tccgggagaa ggtgaactcg gacgcaacca cgtacaagtt ctgtaaggcg caacggccag cgctgcaata cgcggaatgg aggcggcaac agcatgagta cggcgtcaca cccttcgagc ttcagccggt cgaagccgga gagctgacta gcagcgcgag cgtcgccaca aggactctct ttggtgagct cttccagcgc agcgttgtcg cacgcgtggc tgaagggcag gtgcgaatac gcaacggaac gcaccacaac agtcaagaca acggcttcca gcagcccttc gtgcgcatcg acgcgacgtt cgacgagcct ctccccatcc cgttgtag

Details for LemaA.20196.a.B1.GE42328

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	30kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS VEEPQPSSPV AVNDDAEDVV EAELVAYDAH HTHLVEGDFF PAEAPEVEAD GAVADATEEV DALVDAIAAE AAAVAAVSVV STPPKPAEVP SRKRVCWADE GHPDTARGLV KHVTNFYMPN TFSRGSRYDA ADQSSPTRQT NATAVNPTPP LFREKVNSDA TTYKFCKAQR PALQYAEWRR QQHEYGVTPF ELQPVEAGEL TSSASVATRT LFGELFQRSV VARVAEGQVR IRNGTHHNSQ DNGFQQPFVR IDATFDEPLP IPL

Validated NT Sequence

atcttactca cttacaacgg gatggggaga ggctcgtcga acgtcgcgtc gatgcgcacg aagggctgct ggaagccgtt gtcttgactg ttgtggtgcg ttccgttgcg tattcgcacc tgcccttcag ccacgcgtgc gacaacgctg cgctggaaga gctcaccaaa gagagtcctt gtggcgacgc tcgcgctgct agtcagctct ccggcttcga ccggctgaag ctcgaagggt gtgacgccgt actcatgctg ttgccgcctc cattccgcgt attgcagcgc tggccgttgc gccttacaga acttgtacgt ggttgcgtcc gagttcacct tctcccggaa caggggtggg gtcgggttga cggccgtcgc gttggtctgc cgcgtagggc tagattggtc ggcggcgtcg taacgggagc cgcgtgagaa ggtgttgggc atgtagaagt tcgtgacgtg cttcaccaag ccgcgggcgg tgtcggggtg gccttcatcg gcccagcaaa cccgcttgcg ggacggcact tcggccggct ttggcggggt cgacaccacc gacactgcgg ccactgccgc tgcttcggcg gcaatggcgt caacgagcgc gtcgacttcc tcagtagcgt cagcgactgc tccatcagcc tccacctccg gcgcttcggc cgggaaaaag tcgccttcga caaggtgcgt gtgatgcgcg tcgtaagcga ccaactccgc ttccaccacg tcctcggcgt catcgttcac tgcaaccggc gaacttggct gcggctcctc gacgctcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tcgaggagcc gcagccaagt tcgccggttg cagtgaacga tgacgccgag gacgtggtgg aagcggagtt ggtcgcttac gacgcgcatc acacgcacct tgtcgaaggc gactttttcc cggccgaagc gccggaggtg gaggctgatg gagcagtcgc tgacgctact gaggaagtcg acgcgctcgt tgacgccatt gccgccgaag cagcggcagt ggccgcagtg tcggtggtgt cgaccccgcc aaagccggcc gaagtgccgt cccgcaagcg ggtttgctgg gccgatgaag gccaccccga caccgcccgc ggcttggtga agcacgtcac gaacttctac atgcccaaca ccttctcacg cggctcccgt tacgacgccg ccgaccaatc tagccctacg cggcagacca acgcgacggc cgtcaacccg accccacccc tgttccggga gaaggtgaac tcggacgcaa ccacgtacaa gttctgtaag gcgcaacggc cagcgctgca atacgcggaa tggaggcggc aacagcatga gtacggcgtc acacccttcg agcttcagcc ggtcgaagcc ggagagctga ctagcagcgc gagcgtcgcc acaaggactc tctttggtga gctcttccag cgcagcgttg tcgcacgcgt ggctgaaggg caggtgcgaa tacgcaacgg aacgcaccac aacagtcaag acaacggctt ccagcagccc ttcgtgcgca tcgacgcgac gttcgacgag cctctcccca tcccgttgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat