LemaA.19530.a

J-binding protein 1 (JBP1)

CENTER ID: LemaA.19530.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19530.a.B3.GE41623 Tet-JBP 65 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79409
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.09.1480
UniProt: Q4QHM7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPDPKKIKL DIFNFPTARE TRTPEEVAES YAEAVKSHPF YDNVHSVIDF YDSGTIKDGR GQIIGVVLRE ALPKYAVSMA SELLASAAVR TSLRSMMFGG ESPLSGIAGY FDYRGSPVEL KSRKTSFTYE HEAAWPAVFP VVDYVSELYR HVAPERWKAQ NDAIPDVVRI HGTPFSTLTI NSRFRTASHT DVGDFDGGYS CIACLDGHFK GLALAFDDFG INVLMQPRDV MIFDSHHFHS NTEVELSFSG EDWKRLTCVF YYRAALGEPA SYAEYQRRLE KSKQDNSFTP VVSNVRVKEN GTNLNRPSPV YPICPSPFWV PMVAHCLQHC ASEAQCVHDA MTADGSRLAE VMFGEPLSTS DGIPLRGEDK KLKANSDSAS RPLSRLGGFS ETNLMVSTAV EKKKYLNSEF LSHFISAQLL DMWKQARGKW LELVGREWTH MLALNPERKD FLWKNQSEMN SAFFDLCEVG KQVMLGLLGK EVALPKEEQA FWTMYAVHLN AACAEELHMP HVAMSLRKLN VKLKDFNFGG TRYFKDMPPE EQKRRMERKQ RIEEARRHGM SSGAHEKRAN WLTNDSFDYQ TEDCVVDYAQ HKWPPPALHA KEITKNVRTG ELPTREGVVR VLVVLPDPQS KLECVDCKLE VPETVRCSCE WERLMSSLAV RRVLAAAQRN LQLPGSVTHG NIEIRFAFHS RLPTDMCDFV VLQHVLSCIP DDVLASAYIR RAAALCTGCV YVVETDVQCR QYYTLKCAAR CDYDAVASLF FQQLHRVSYG TKAARVRTKG ELESLIPTVC CARYKLQGSP LNTTVHVVSP APSR
NT Sequence
atggagcctg acccaaagaa gatcaaactg gacatcttta acttcccgac cgccagggag acacggacgc cggaggaagt ggcagagagc tacgcggaag ccgtcaagtc gcaccctttt tacgacaacg tgcactctgt gatcgacttt tacgatagcg gtaccatcaa agatggtcgt gggcaaatca ttggtgttgt gctgcgagaa gccctaccaa agtacgcggt atcgatggcg tcggagctgc tggcatccgc tgccgtgcgc acgtcgcttc ggagcatgat gttcggcgga gagtcaccct tgagcggtat tgctggctac ttcgactacc gcggctcacc agtggagctg aagtcacgca aaacgtcctt cacatatgag cacgaggcag cgtggccggc ggtcttccct gtcgtcgatt acgtcagcga gctctacagg catgtcgcac cggagcggtg gaaagcacaa aacgacgcca ttccggatgt ggtgcgcatt cacggaaccc cgttctccac gctcacaatc aactcccgct tccgcacagc ctctcacacc gacgttggcg attttgacgg cggctacagc tgcattgctt gcctggacgg gcacttcaaa gggctggccc ttgccttcga cgacttcggc attaacgtgc tcatgcagcc gcgcgatgtg atgatattcg actcgcacca cttccactca aacacggagg tggagttgtc gttctcaggg gaggactgga agcgacttac ctgcgtattc tactaccggg ccgccctcgg tgagccggcg agctacgccg agtaccagcg ccggttggag aagtccaagc aggacaacag cttcactcca gtggtgagca acgtgagggt taaagagaac ggcacaaacc taaaccgtcc atcccctgta tatcctatct gtccctcgcc gttttgggtg ccgatggtgg cgcattgcct gcagcactgc gcatcggagg cacaatgcgt tcacgacgcc atgacggcgg atgggagtcg actggctgag gttatgtttg gggaaccgct gtctacctca gacggcatcc cactgcgcgg agaggacaag aagctgaagg ccaacagtga cagcgcgtcc aggccgctct cgcgtctggg tgggttcagc gagacgaatc tgatggtgtc cacggcagta gaaaagaaga agtacctcaa cagcgagttc ctctcccact tcatcagcgc gcagctgctg gacatgtgga agcaggcacg cggcaagtgg ctggagctgg taggcaggga atggacgcac atgctggccc tgaaccccga gcgcaaagac tttttgtgga agaaccagtc cgagatgaac tcggcttttt tcgacctctg cgaggtgggg aagcaggtca tgctcggcct tctggggaag gaggtggctc tgccaaagga agagcaggct ttctggacca tgtacgccgt gcacttgaat gcggcgtgcg cggaggagct gcacatgccg cacgtcgcca tgtcacttcg caagctcaac gtgaagctga aggacttcaa ctttggcggc acgcgatatt tcaaagacat gcctcccgag gagcagaagc gcagaatgga gcgcaagcag cgcattgagg aggcacgccg ccacggcatg tcgtctggcg cacacgaaaa gcgagcgaac tggctgacca acgactcatt cgactaccaa actgaggact gtgtggttga ttacgcccag cacaagtggc cgccgcctgc gcttcacgca aaggagataa cgaagaacgt gcgcactggc gagctgccaa ctagggaagg tgtggtgcgg gtgctcgttg tgctgcccga tccgcaaagc aagttggagt gtgtggactg caagctggag gtgccggaaa cggtgagatg ttcatgcgag tgggagcgtt tgatgagcag cctggctgtg cgtcgtgtcc tagccgctgc gcagcgcaac ctgcagctgc ctggcagcgt cacgcatggc aacatcgaga tccgctttgc cttccatagc aggctgccta cggacatgtg cgactttgtg gtgctgcagc atgtcttgag ctgcatccca gatgacgtgc tggcgtcggc gtatatcaga cgcgctgcag cgctctgcac cggctgtgtg tatgtggtgg agacggacgt gcaatgccga caatactaca ccctcaagtg tgccgctcgc tgcgactacg acgcggtcgc ctcattattc ttccagcagc tgcaccgggt gagttacggg acgaaagcgg cacgcgtgcg taccaaggga gaactggagt ctcttatccc cacggtgtgc tgtgcgcgct acaagctgca aggctcgccg ctgaacacga ccgttcatgt cgtgtctccc gctccgtccc gctga
Details for LemaA.19530.a.B3.GE41623
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG VVLREALPKY AVSMASELLA SAAVRTSLRS MMFGGESPLS GIAGYFDYRG SPVELKSRKT SFTYEHEAAW PAVFPVVDYV SELYRHVAPE RWKAQNDAIP DVVRIHGTPF STLTINSRFR TASHTDVGDF DGGYSCIACL DGHFKGLALA FDDFGINVLM QPRDVMIFDS HHFHSNTEVE LSFSGEDWKR LTCVFYYRAA LGEPASXXXY XXXLEXXSK
Validated NT Sequence
atcttactcn nntactccaa nnngcnnngg tactnnnnnt agctcgccgg ctcaccgagg gcggcccggt agtagaatac gcaggtaagt cgcttccagt cctcccctga gaacgacaac tccacctccg tgtttgagtg gaagtggtgc gagtcgaata tcatcacatc gcgcggctgc atgagcacgt taatgccgaa gtcgtcgaag gcaagggcca gccctttgaa gtgcccgtcc aggcaagcaa tgcagctgta gccgccgtca aaatcgccaa cgtcggtgtg agaggctgtg cggaagcggg agttgattgt gagcgtggag aacggggttc cgtgaatgcg caccacatcc ggaatggcgt cgttttgtgc tttccaccgc tccggtgcga catgcctgta gagctcgctg acgtaatcga cgacagggaa gaccgccggc cacgctgcct cgtgctcata tgtgaaggac gttttgcgtg acttcagctc cactggtgag ccgcggtagt cgaagtagcc agcaataccg ctcaagggtg actctccgcc gaacatcatg ctccgaagcg acgtgcgcac ggcagcggat gccagcagct ccgacgccat cgataccgcg tactttggta gggcttctcg cagcacaaca cccatatggt ggtggtggtg gtgagccatg gtatat
Expected Protein Sequence
MAHHHHHHGV VLREALPKYA VSMASELLAS AAVRTSLRSM MFGGESPLSG IAGYFDYRGS PVELKSRKTS FTYEHEAAWP AVFPVVDYVS ELYRHVAPER WKAQNDAIPD VVRIHGTPFS TLTINSRFRT ASHTDVGDFD GGYSCIACLD GHFKGLALAF DDFGINVLMQ PRDVMIFDSH HFHSNTEVEL SFSGEDWKRL TCVFYYRAAL GEPASYAEYQ RRLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtgttg tgctgcgaga agccctacca aagtacgcgg tatcgatggc gtcggagctg ctggcatccg ctgccgtgcg cacgtcgctt cggagcatga tgttcggcgg agagtcaccc ttgagcggta ttgctggcta cttcgactac cgcggctcac cagtggagct gaagtcacgc aaaacgtcct tcacatatga gcacgaggca gcgtggccgg cggtcttccc tgtcgtcgat tacgtcagcg agctctacag gcatgtcgca ccggagcggt ggaaagcaca aaacgacgcc attccggatg tggtgcgcat tcacggaacc ccgttctcca cgctcacaat caactcccgc ttccgcacag cctctcacac cgacgttggc gattttgacg gcggctacag ctgcattgct tgcctggacg ggcacttcaa agggctggcc cttgccttcg acgacttcgg cattaacgtg ctcatgcagc cgcgcgatgt gatgatattc gactcgcacc acttccactc aaacacggag gtggagttgt cgttctcagg ggaggactgg aagcgactta cctgcgtatt ctactaccgg gccgccctcg gtgagccggc gagctacgcc gagtaccagc gccggttgga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat