LemaA.19528.a

J-binding protein 2 (JBP2)

CENTER ID:	LemaA.19528.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.19528.a.B5.GE41620	helicase domain		543	1098

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79434

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.14.0040
UniProt:	Q4QFY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLNGLTRVST SSELESILDI VQSSGEIAVV FTSPSIGDLE TIASETQRRQ LRIAGIPRGG YTILPAIPLY DDELLQMCER YTAANEYEKA EMRNSLYMRE YPLFAYSMRH QRALFHPADY VSRILQFCSY YVQAPDADVL SLQDRSPFLH ISPIKEICTQ LRLIARGTPV AASDSESPVP EQLRLHAESD VEKLAAERAT AMSIAASSGG ASETEQLSLF SGVAPSALFQ KDAVEEVNKD AEDTMEDLTG EETVDAVHSF QAEYLTLDGF ELVTKASIFY DREGEGQCIV AVYIPGGVPE DTCRAAAAVL EPAATKKNLR APTNGGLPPD TGIVGYYDYL TNPTQHKCRE TEFSRRNWGL LAQSEPLLKH LDKLYSQLAP MHHHLQRVAI PSQYQLCGTV FSTITVNRNF RTAVHTDRGD FRSGLGVLSV INGEFEGCHL AIKRLKKAFQ LKVGDVLLFD TSLEHGNTEV VNPEIHWQRT SVVCYLRTGL MSSVCEMERR KHLNRLILEQ LLNTEVRNTT VNINGADSSL PPLFVPTRLA SHLAPVQLAA LGFIVERTEK QSGCVVAMTM GLGKTLVALT LCFSQLYLAP QADILILTPK PIISHWVDEK NKWGMHGLHF PHFVASDGLN SLEFEQQLLE YERQKNNEKP KSGHIFVING EYLAGFLRRF KRFTPLVMIV DEGHRVAAKG NKLTESLDRL RCNLRIVLSG TPLQNDASEL YRLVGWVNKG VSRVLPPKRF QELANDINQF VEGDDGAFYN AVVAQEYIQD WMRGFVFREM ENDLPPLHDY LLICGSSDVQ REYEEKLGLT ETAMTALKAT EHRPHHLSTH PACYLAFISD SYQSMVSGWT VRALSNTSRQ RVSQLEEIDT MRLEQYVQLV ENEQLDAFID LSGKMRVLVD IVLRVQARKE KLIVFSLYVG SQDLIHRTLT ALRVCTFTVR GRDSQDRRRR AMQEFSENKD LIVLVLSTKI AAYGLDFTAA NHVVLFDSWW NPQVDAQAIA RAYRRNQRKP VTVYRLISAT ENKFVLRSQT RKIALFKCIL HERTSRQALP DELEDCAANE KDEERRNFWA KLKMTSLAGD TRALLNVYRY QESVRESE

NT Sequence

atgttaaatg ggctgacgcg ggtgagcacc agttctgagc tggagtcgat actagatatc gtgcaatcgt ccggcgaaat cgcggtcgta ttcacttcgc cgagcattgg cgatttggag acgattgcaa gtgaaacaca gcgtcgtcag ctgcgcattg cgggaatccc tcgcggcggg tacaccatcc tccctgctat tccgctatac gatgacgagc tgcttcagat gtgtgagcgg tacaccgcag cgaacgagta cgagaaggcg gaaatgcgca acagcttgta tatgcgagaa tacccactat tcgcatacag catgcgtcat cagcgagcac tatttcaccc tgccgactac gtgagccgga tactccagtt ctgctcctat tatgtccagg cgcccgacgc agacgttttg tcactgcagg acagaagtcc ttttcttcac atttccccca taaaggagat ttgcacgcaa ctccgcctca ttgcccgcgg caccccagtg gcagcaagcg actccgagtc gccggtgcca gagcagctgc gccttcatgc agagtcagat gtggagaagc ttgcagccga gcgtgccacc gctatgtcta tcgcagccag tagtggtggc gcctcggaga cggagcagct gtctctcttc agcggcgttg cgccgtcggc gctgtttcag aaagatgcag tggaggaggt caacaaggac gctgaagaca ccatggagga tctcaccggc gaggagacag tggacgcggt gcacagcttt caggcagagt acttgacact ggacgggttt gagctggtga caaaagcatc aatcttctac gaccgcgaag gggaggggca gtgcatcgtc gcggtgtaca ttccgggagg ggtaccagag gacacgtgcc gcgcagccgc agcggtcctg gagccggcgg cgacaaaaaa gaatttgcgg gcacccacga acggtggact gccaccagac accgggatcg tgggctacta cgactacctt acgaatccta ctcagcacaa gtgcagagag acggaattca gccgcaggaa ttgggggctc ctagcgcaga gcgagccgct gctgaagcat ctcgacaagc tgtacagtca gttggctcca atgcaccatc atctgcagag ggtggccatc ccgtcgcagt accagctgtg cggaacagtc ttcagcacca tcactgttaa cagaaacttc cgcaccgccg tgcataccga caggggtgac tttcggagcg gcttgggggt tctctcagtt atcaacggcg agtttgaggg atgtcatctc gccatcaaaa ggctcaagaa ggcgtttcag ctcaaagtcg gtgacgttct tcttttcgac acgtctctgg agcacggcaa cacggaggtg gtaaacccgg aaatccactg gcagcgaacc agcgttgtgt gctacctgcg cactggacta atgtcctctg tgtgcgagat ggagcgacgt aagcacctca atcggctcat tctcgagcag ctgctcaaca cggaggttcg caacacgacg gtgaacatca acggtgccga cagcagcctg ccaccgctgt ttgtgccgac tcgcctggcg agccatttgg cgcccgtgca gcttgctgcc ctcggcttca ttgtggagcg gacggagaag cagagtgggt gcgtcgtggc aatgacgatg ggactgggca aaacgcttgt tgcactcacg ttgtgcttct cacagctgta tcttgcaccg caggcagaca tactcatcct cacgccaaaa ccgatcatca gccactgggt ggacgagaaa aacaagtggg gcatgcacgg actccacttt ccgcacttcg tcgcctccga cgggctcaac tcgttggagt tcgagcagca gcttcttgag tacgagcgtc agaagaacaa cgagaaaccc aagtcgggcc acatcttcgt catcaacggc gagtacctag cagggtttct gcggcgtttc aagcgcttta cgcccttggt catgatcgtg gacgagggcc accgagtggc tgcgaagggg aataagttga cagagtccct cgatcgccta cggtgcaacc tccgcatcgt gctatccggc acgcctttgc aaaacgacgc cagcgaactc taccggctag tggggtgggt gaacaaaggc gtcagtaggg tactgccgcc gaagcgcttc caggagctcg cgaatgacat caaccagttc gttgaaggcg acgatggggc cttctacaat gcagtggtgg cgcaggagta catccaggac tggatgcgtg ggttcgtgtt tcgtgaaatg gaaaacgacc tgccaccgct gcacgattac ctgttgattt gcggctcctc cgacgtgcag cgggagtatg aggagaagct gggcctcacg gagacggcca tgacagccct aaaagccacg gagcaccgac cgcatcacct ctccacgcac cccgcttgct acctggcctt catttccgac agctaccaat cgatggtaag cgggtggaca gtgcgcgcgc tgtcgaacac gtcgcggcag cgggtgtccc aactggagga aattgatacc atgcgactgg agcagtatgt ccagttggtc gagaacgagc aacttgacgc cttcatcgac ttgagcggca agatgcgggt gctcgtcgat attgtgctgc gcgtgcaagc ccgcaaggag aagctcatcg ttttctctct ctacgtaggt tcgcaggacc tgattcatcg caccctgacg gcgctgcggg tctgcacatt taccgttcgc ggccgtgact cgcaagaccg ccgccgccgc gccatgcagg agttcagcga gaataaagat ctcattgtgc tggtattatc gacaaagatc gctgcctacg gtctcgactt cacagcggcg aatcacgttg ttttgttcga ctcgtggtgg aacccgcagg tggatgcaca ggccatcgcg cgcgcgtaca ggcgaaatca acggaagcca gtgaccgtgt atcgccttat ctcagcaacc gaaaataagt tcgtgctgcg ctcacagaca cgaaagattg ccctcttcaa gtgcatcctc catgagcgca ccagtcggca ggcgttgccc gacgagctgg aggattgcgc agcgaacgag aaggatgaag aaaggcggaa cttttgggca aaactgaaga tgacctctct cgctggggat actcgggcgt tgttgaacgt gtatcgctac caggagagcg ttcgcgaaag cgaatga

Details for LemaA.19528.a.B5.GE41620

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	65kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	73
PERCENT COVERAGE:	59

Validated AA Sequence

MAHHHHHHML APVQLAALGF IVERTEKQSG CVVAMTMGLG KTLVALTLCF SQLYLAPQAD ILILTPKPII SHWVDEKNKW GMHGLHFPHF VASDGLNSLE FEQQLLEYER QKNNEKPKSG HIFVINGEYL AGFLRRFKRF TPLVMIVDEG HRVAAKGNKL TESLDRLRCN LRIVLSGTPL QNDASELYRL VGWVNKGVSR VLPPKRFQEL ANDINXXVEG DDGAFXXXXV AXEXXXDWMX XFVFRXXXXX LXXXXXYXXI CXXXXXXXXX XXXXXXXXXX XXXXXXXXXA XXASPLHAPR LXPGLHFRXX XXXGKRVDSA RAXXHVAAAG VPTGGN

Validated NT Sequence

gcgatacacg ttcaacaacg cccgagtatc cccagcgaga gaggtcatct tcagttttgc ccaaaagttc cgcctttctt catccttctc gttcgctgcg caatcctcca gctcgtcggg caacgcctgc cgactggtgc gctcatggag gatgcacttg aagagggcaa tctttcgtgt ctgtgagcgc agcacgaact tattttcggt tgctgagata aggcgataca cggtcactgg cttccgttga tttcgcctgt acgcgcgcgc gatggcctgt gcatccacct gcgggttcca ccacgagtcg aacaaaacaa cgtgattcgc cgctgtgaag tcgagaccgt aggcagcgat ctttgtcgat aataccagca caatgagatc tttattctcg ctgaactcct gcatggcgcg gcggcggcgg tcttgcgagt cacggccgcg aacggtaaat gtgcagaccc gcagcgccgt cagggtgcga tgaatcaggt cctgcgaacc tacgtagaga gagaaaacga tgagcttctc cttgcgggct tgcacgcgca gcacaatatc gacgagcacc cgcatcttgc cgctcaagtc gatgaaggcg tcaagttgct cgttctcnnn naactggaca tactgctcca gtcgcatggt atcaatttcc tccagttggg acacccgctg ccgcgacgtg ttnnncagcg cgcgcactgt ccacccgctt accatnnnnn nnnnnnngtc ggaaatgaag gccaggnngc aagcggggtg cgtggagagg tgatgcgnnn nnngctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng caaatnnnnn ggtaatnnnn nngngnnnnc agnnnnnnnn nnatntcacg aaacacgaac nnnnncatcc agtcnnnnnn nnactcnngc gccacnnntn nnnnntagaa ggccccatcg tcgccttcaa cgannnggtt gatgtcattc gcgagctcct ggaagcgctt cggcggcagt accctactga cgcctttgtt cacccacccc actagccggt agagttcgct ggcgtcgttt tgcaaaggcg tgccggatag cacgatgcgg aggttgcacc gtaggcgatc gagggactct gtcaacttat tccccttcgc agccactcgg tggccctcgt ccacgatcat gaccaagggc gtaaagcgct tgaaacgccg cagaaaccct gctaggtact cgccgttgat gacgaagatg tggcccgact tgggtttctc gttgttcttc tgacgctcgt actcaagaag ctgctgctcg aactccaacg agttgagccc gtcggaggcg acgaagtgcg gaaagtggag tccgtgcatg ccccacttgt ttttctcgtc cacccagtgg ctgatgatcg gttttggcgt gaggatgagt atgtctgcct gcggtgcaag atacagctgt gagaagcaca acgtgagtgc aacaagcgtt ttgcccagtc ccatcgtcat tgccacgacg cacccactct gcttctccgt ccgctccaca atgaagccga gggcagcaag ctgcacgggc gccaacatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHLA PVQLAALGFI VERTEKQSGC VVAMTMGLGK TLVALTLCFS QLYLAPQADI LILTPKPIIS HWVDEKNKWG MHGLHFPHFV ASDGLNSLEF EQQLLEYERQ KNNEKPKSGH IFVINGEYLA GFLRRFKRFT PLVMIVDEGH RVAAKGNKLT ESLDRLRCNL RIVLSGTPLQ NDASELYRLV GWVNKGVSRV LPPKRFQELA NDINQFVEGD DGAFYNAVVA QEYIQDWMRG FVFREMENDL PPLHDYLLIC GSSDVQREYE EKLGLTETAM TALKATEHRP HHLSTHPACY LAFISDSYQS MVSGWTVRAL SNTSRQRVSQ LEEIDTMRLE QYVQLVENEQ LDAFIDLSGK MRVLVDIVLR VQARKEKLIV FSLYVGSQDL IHRTLTALRV CTFTVRGRDS QDRRRRAMQE FSENKDLIVL VLSTKIAAYG LDFTAANHVV LFDSWWNPQV DAQAIARAYR RNQRKPVTVY RLISATENKF VLRSQTRKIA LFKCILHERT SRQALPDELE DCAANEKDEE RRNFWAKLKM TSLAGDTRAL LNVYRYQESV RESE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggcgc ccgtgcagct tgctgccctc ggcttcattg tggagcggac ggagaagcag agtgggtgcg tcgtggcaat gacgatggga ctgggcaaaa cgcttgttgc actcacgttg tgcttctcac agctgtatct tgcaccgcag gcagacatac tcatcctcac gccaaaaccg atcatcagcc actgggtgga cgagaaaaac aagtggggca tgcacggact ccactttccg cacttcgtcg cctccgacgg gctcaactcg ttggagttcg agcagcagct tcttgagtac gagcgtcaga agaacaacga gaaacccaag tcgggccaca tcttcgtcat caacggcgag tacctagcag ggtttctgcg gcgtttcaag cgctttacgc ccttggtcat gatcgtggac gagggccacc gagtggctgc gaaggggaat aagttgacag agtccctcga tcgcctacgg tgcaacctcc gcatcgtgct atccggcacg cctttgcaaa acgacgccag cgaactctac cggctagtgg ggtgggtgaa caaaggcgtc agtagggtac tgccgccgaa gcgcttccag gagctcgcga atgacatcaa ccagttcgtt gaaggcgacg atggggcctt ctacaatgca gtggtggcgc aggagtacat ccaggactgg atgcgtgggt tcgtgtttcg tgaaatggaa aacgacctgc caccgctgca cgattacctg ttgatttgcg gctcctccga cgtgcagcgg gagtatgagg agaagctggg cctcacggag acggccatga cagccctaaa agccacggag caccgaccgc atcacctctc cacgcacccc gcttgctacc tggccttcat ttccgacagc taccaatcga tggtaagcgg gtggacagtg cgcgcgctgt cgaacacgtc gcggcagcgg gtgtcccaac tggaggaaat tgataccatg cgactggagc agtatgtcca gttggtcgag aacgagcaac ttgacgcctt catcgacttg agcggcaaga tgcgggtgct cgtcgatatt gtgctgcgcg tgcaagcccg caaggagaag ctcatcgttt tctctctcta cgtaggttcg caggacctga ttcatcgcac cctgacggcg ctgcgggtct gcacatttac cgttcgcggc cgtgactcgc aagaccgccg ccgccgcgcc atgcaggagt tcagcgagaa taaagatctc attgtgctgg tattatcgac aaagatcgct gcctacggtc tcgacttcac agcggcgaat cacgttgttt tgttcgactc gtggtggaac ccgcaggtgg atgcacaggc catcgcgcgc gcgtacaggc gaaatcaacg gaagccagtg accgtgtatc gccttatctc agcaaccgaa aataagttcg tgctgcgctc acagacacga aagattgccc tcttcaagtg catcctccat gagcgcacca gtcggcaggc gttgcccgac gagctggagg attgcgcagc gaacgagaag gatgaagaaa ggcggaactt ttgggcaaaa ctgaagatga cctctctcgc tggggatact cgggcgttgt tgaacgtgta tcgctaccag gagagcgttc gcgaaagcga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat