LemaA.19527.a

Glucosyl transferase (HmdUGT)

CENTER ID: LemaA.19527.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19527.a.B3.GE41615 C-term domain 807 951
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79426
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.36.2370
UniProt: Q4Q1I4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSLNIKTAA STSSAAPTPA PGVNAGEAPW ATIDVDEADS FESTPSTNSS GNQSGRHHYQ HAAQPSHSPQ SPETPIKFDH GVYPHCPIFI PSKGRSDLDR STMAVLVRDL VPFVLVVERE EAEGYRAMLD RLVGQYFGFA GMTFAASVDD GIEGATKTGA GVVESSSTTP ADLWAFPCAC CCGSTSVAGS PSSPTTPGLS ADALRALALR LSAAFHHGYV FEPTSAAAGN HHGAVESKNV SPPVSISPTT PSAASGGARR PSAAFVLRNV DEVRALFVIE VLPETNRGVS YVRNYILQVL VPRLMVACGV DRSGNVEELR LCGPGEHNTL TPAALRMAAL EPDVYSALEW KSETESYLDV RRASTTTNMV TASTGSAAGT KDGGVSASVG ALSAPTGQTL APALSSSSAP RSSTASSAAA ATRGTPRLLH GLFGFYWVLD DDIYGFYQSH GSSTKNERIS TRTMMREVEV RLRQLRHNAD VASPPPPSVP PSCFQTPPTP SSGSAAASPY VLSNQQQQQN FVFTCKEDAL QNNIISGGKG RLPTLQNYSL YYTTACFSLE YNRFALDTAP DTLSVNSYNN IACLFNYALL HNPPQMRYFP PGVPHVRRPE DALVGYLDHS MLWYRFAVRE DYDFTLQLIA RGLHTLRFRS IAFDVPQMMK VRGGMTDYYR NCHEEIRQQN DRFVLQWPAV AQHWVKGKEG SRRDDIRVRW DLLSPARTKH PGAFLYLSAP LPQLQPQRLS DDSTGGNGSV ISSSDSAATA TAVEGTETET RKRLRPPSPT APTAESSHAP AGRRPCVPAA TASAPAMSST VARDWKGGYV VERWRDISRE EARQHAGLVA LSNDDIHIGR TVAVIPPFFD QKPSVVRATV IERAVEPTDS HASSGRAGNT STAEAGDAQQ SLTPRVIWTA VAQDVRGMPF LHVDTCFEVP AEGIEKAAAS IDQFFAKMES EPGS
NT Sequence
atgttctccc tcaacatcaa aacagcggcc tcgacgtcct ctgcagctcc cacaccagca ccgggagtga acgccggcga ggcgccatgg gcgaccattg acgtggacga agccgactcc tttgaaagca caccctcgac gaacagcagc ggtaatcaaa gtggccgcca tcattatcag catgccgccc agccgtcgca ctctccgcag agtccggaga cgcccatcaa atttgaccac ggtgtatacc cgcactgccc catcttcatc ccctccaaag gtcgttcaga cctggaccgc agcactatgg cggtgctcgt ccgcgatctc gtcccgtttg tgcttgtcgt cgagcgggag gaggccgagg ggtacagagc catgctggac cgcctcgttg gacaatactt cggcttcgcc ggtatgacgt ttgccgcgag cgtagatgat ggcattgaag gggcgaccaa gacgggggca ggggtcgtcg agtcgtcgag caccactccg gcagacctct gggcgtttcc gtgcgcctgc tgctgcggca gcactagcgt cgctggttcg ccgtcgtcgc ccaccacgcc cggcctatcg gcagatgcct tgcgcgcgtt ggcgctgcgg ctttcagctg cgtttcatca tggctatgtg ttcgagccaa cgtctgctgc agccggcaac caccacggcg ctgtcgagtc gaagaatgtc tcaccgcctg tgtcgatcag tccaacaacg ccatcagcag ccagcggggg tgcgcggcgc ccctctgcgg cctttgtgct gcgcaacgtg gacgaggtgc gcgcgctctt cgtgatcgaa gtgctaccgg agaccaatcg tggggtctcc tatgtgcgca actacatcct acaggttctc gtgccaaggc tgatggtggc ctgcggcgta gatcggtcag ggaacgtgga ggagctgcgc ctgtgtggcc cgggagagca caacactctg acaccggcgg cgctgcggat ggccgcgctg gagccagacg tctacagtgc ccttgagtgg aagtcagaga ccgagtccta tctggacgta cggcgggcga gcaccaccac gaacatggta acggcaagca ctggcagcgc cgcgggcacc aaagatggcg gcgtgtcggc gtcggtggga gcgttgagcg ccccaactgg acaaactttg gcaccagcgt tgtcgtcctc gtctgcgcca cgttcgtcga cggcgtcctc cgctgcggcc gcgactcgcg gcacgcctcg ccttctgcac ggcctcttcg gcttctactg ggtcctggat gacgatatct acggctttta ccagtcgcac ggctccagca ccaaaaacga gcgcattagt acgcggacaa tgatgagaga ggtggaggtg cggcttcggc aactgcggca caacgccgac gtggcctcgc caccaccgcc gtcagttccg ccctcgtgct ttcagacacc gccgaccccc agcagcggca gcgcagccgc gtcgccgtat gtcctgtcaa accagcagca acagcaaaat ttcgtgttca cgtgcaagga ggatgcccta cagaacaaca tcatctctgg agggaaaggc cggcttccga cgctgcagaa ctacagcctc tactacacaa ccgcatgctt ctcgctcgag tacaaccgct tcgccctcga cacggctccg gacacgctgt cggtcaactc ctacaataac attgcctgtc ttttcaacta cgcgctgctg cacaacccgc cgcagatgcg ctacttcccc cctggagtgc cgcacgtgag gcgccccgag gacgcgctgg tcggctacct cgatcacagt atgctctggt accgcttcgc cgtgcgcgag gattacgact tcactcttca gctcatcgca cgtggcctcc acacgctgcg cttccgcagc atcgccttcg atgtgccgca gatgatgaag gtgcgtggcg gcatgacgga ctactaccgc aactgtcacg aagagatacg gcagcagaac gatcgctttg tgctgcagtg gccggccgtg gcgcagcact gggtgaaggg gaaggagggc agccgtcgag atgacatacg tgtgcggtgg gacctgctga gcccagcccg caccaagcac cccggtgcct tcctgtatct gagcgcccca ctgccgcagc tgcagccgca acgcttgagt gacgacagca ccggcggaaa cggcagtgta attagcagca gcgacagtgc agccactgca accgcggtag aaggcacgga aacagagacg cgcaagcgtc tgcgaccccc gtccccgact gctcccactg ctgaatcgtc ccatgcccct gcaggccgtc gcccctgcgt cccagccgca acagcgtctg cgcccgctat gtcgtccacc gtcgctcgtg actggaaagg tggctacgtg gtcgagcggt ggcgtgatat ctcgcgtgag gaggcgcgac agcacgccgg cctcgtcgcc ctctccaacg acgacatcca cattggccgg actgtcgccg tcataccacc cttcttcgac cagaaaccat ctgtcgtgcg cgcaacagta attgagcggg ccgtcgagcc gacggactcc catgccagca gcggccgcgc tgggaacacc agcacggcgg aagcaggtga tgcccagcag tccctaacgc cgcgcgtgat ctggacggcg gtggcacaag atgttcgtgg tatgcctttc ttgcacgttg acacctgctt tgaggtgccc gcggagggca tcgaaaaggc cgccgccagc atcgatcagt tcttcgcaaa gatggagagc gaacctggct catag
Details for LemaA.19527.a.B3.GE41615
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS STVARDWKGG YVVERWRDIS REEARQHAGL VALSNDDIHI GRTVAVIPPF FDQKPSVVRA TVIERAVEPT DSHASSGRAG NTSTAEAGDA QQSLTPRVIW TAVAQDVRGM PFLHVDTCFE VPAEGIEKAA ASIDQFFAKM ESE
Validated NT Sequence
atcttactca cttattcgct ctccatcttt gcgaagaact gatcgatgct ggcggcggcc ttttcgatgc cctccgcggg cacctcaaag caggtgtcaa cgtgcaagaa aggcatacca cgaacatctt gtgccaccgc cgtccagatc acgcgcggcg ttagggactg ctgggcatca cctgcttccg ccgtgctggt gttcccagcg cggccgctgc tggcatggga gtccgtcggc tcgacggccc gctcaattac tgttgcgcgc acgacagatg gtttctggtc gaagaagggt ggtatgacgg cgacagtccg gccaatgtgg atgtcgtcgt tggagagggc gacgaggccg gcgtgctgtc gcgcctcctc acgcgagata tcacgccacc gctcgaccac gtagccacct ttccagtcac gagcgacggt ggacgacata tggtggtggt ggtggtgagc catggtatat
Expected Protein Sequence
MAHHHHHHMS STVARDWKGG YVVERWRDIS REEARQHAGL VALSNDDIHI GRTVAVIPPF FDQKPSVVRA TVIERAVEPT DSHASSGRAG NTSTAEAGDA QQSLTPRVIW TAVAQDVRGM PFLHVDTCFE VPAEGIEKAA ASIDQFFAKM ESE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt ccaccgtcgc tcgtgactgg aaaggtggct acgtggtcga gcggtggcgt gatatctcgc gtgaggaggc gcgacagcac gccggcctcg tcgccctctc caacgacgac atccacattg gccggactgt cgccgtcata ccacccttct tcgaccagaa accatctgtc gtgcgcgcaa cagtaattga gcgggccgtc gagccgacgg actcccatgc cagcagcggc cgcgctggga acaccagcac ggcggaagca ggtgatgccc agcagtccct aacgccgcgc gtgatctgga cggcggtggc acaagatgtt cgtggtatgc ctttcttgca cgttgacacc tgctttgagg tgcccgcgga gggcatcgaa aaggccgccg ccagcatcga tcagttcttc gcaaagatgg agagcgaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat