SSGCID: Target Page

LemaA.19526.a

WDR82 homologue (glucosyl transferase interacting protein 1) (GTIP1)

CENTER ID:	LemaA.19526.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.19526.a.B1.GE41474	full length		1	391
LemaA.19526.a.B2.GE41475	WD-40 propeller domain		25	391

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.32.3820
UniProt:	Q4Q4P0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNSASPSPP LTSSGGAAGS AAVDGVPIEV ASMFYTGINE PRPTAMNFSF DGLHFVSAHT DDAVRFIDVG NMTNTDVIIC DAFGVHNVRY THSSSVVCVA PRFHLDGHLH LLNTETSQFF GEMAYLNDAE SEMLPVANTP VYSTLAQCPA TDVIGAVVVA KGRLALFHPL ISGCIAATPE RSIVGSRACV QFSRDGHRIA IGDDHRIVLL DRRSFFGAPL AVLENKHVFT TSTSLTRCKG LEFSADANRL LLTSSSGEVV VYDWLRGETV ANYFHGDARR HFVGSADAIG AQHVFPYQSA SPILQLTSSM TGGRHLLVYA GCPSNSAAST RQGRLLYELQ SKDSDVPVAI AVNPRLQLAA TAARSITWWA FHNSVVEAYA TLTEAGMTAA D

NT Sequence

atgaacaact ccgcctcccc gagccctccc ctcacctcca gcggtggagc agctggctcg gctgctgtgg acggagtacc gatagaggtg gcgtccatgt tctacacagg catcaacgag ccgcggccca ccgcgatgaa cttctccttc gacggccttc acttcgtgtc ggcgcacacg gacgacgccg tccggttcat cgacgtgggc aacatgacga acacggacgt gattatctgc gacgcgtttg gcgtgcacaa cgtccgttac acccactcca gcagtgtcgt atgtgtcgcg ccacggtttc acctggatgg ccacctgcac ctcctgaaca cggagacgtc gcagttcttc ggcgagatgg cgtaccttaa cgacgccgag tcggagatgt tgccagtggc gaacacgcca gtgtactcca cccttgccca gtgcccggca acagacgtga tcggcgcggt ggtagtggcc aaaggtcggc tagccctctt tcaccctctc atctccggct gcattgccgc cacgccggag cggtccattg taggcagtag agcctgcgtg cagtttagcc gggacggcca tcggatcgcc atcggcgacg accaccgcat tgttttactc gatcgacgca gctttttcgg ggcaccgctg gccgtgctcg agaacaagca tgtctttacc acgtcgacgt cgctgacgag atgcaaaggg ctcgagttta gcgccgatgc gaatcgcctg ctgctcacat cgtcgagtgg tgaagttgtc gtatacgact ggctgcgcgg cgagacggtg gcgaactact tccacggcga cgcgaggcga cattttgtcg ggtctgccga cgccattggc gcgcagcacg tcttccctta ccagtcagcc tcgcccattc tgcagctcac ctcgtccatg accggaggcc ggcatctgct tgtgtacgcc ggatgcccgt ccaactcggc agcctccacc cgacaaggac gacttctgta tgagctgcag tcgaaggaca gcgatgtgcc ggtcgctatc gccgtgaacc cacgactaca gctggcggct actgcagcgc ggagtataac ctggtgggct tttcacaatt ctgttgtgga ggcgtacgcg acactgacgg aggcgggcat gacggcagcc gactga

Details for LemaA.19526.a.B1.GE41474

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN NSASPSPPLT SSGGAAGSAA VDGVPIEVAS MFYTGINEPR PTAMNFSFDG LHFVSAHTDD AVRFIDVGNM TNTDVIICDA FGVHNVRYTH SSSVVCVAPR FHLDGHLHLL NTETSQFFGE MAYLNDAESE MLPVANTPVY STLAQCPATD VIGAVVVAKG RLALFHPLIS GCIAATPERS IVGSRACVQF SRDGHRIAIG DDHRIVLLDR RSFFGAPLAV LENKHVFTTS TSLTRCKGLE FSADANRLLL TSSSGEVVVY DWLRGETVAN YFHGDARRHF VGSADAIGAQ HVFPYQSASP ILQLTSSMTG GRHLLVYAGC PSNSAASTRQ GRLLYELQSK DSDVPVAIAV NPRLQLAATA ARSITWWAFH NSVVEAYATL TEAGMTAAD

Validated NT Sequence

atggctcacc accaccacca ccatatgaac aactccgcct ccccgagccc tcccctcacc tccagcggtg gagcagctgg ctcggctgct gtggacggag taccgataga ggtggcgtcc atgttctaca caggcatcaa cgagccgcgg cccaccgcga tgaacttctc cttcgacggc cttcacttcg tgtcggcgca cacggacgac gccgtccggt tcatcgacgt gggcaacatg acgaacacgg acgtgattat ctgcgacgcg tttggcgtgc acaacgtccg ttacacccac tccagcagtg tcgtatgtgt cgcgccacgg tttcacctgg atggccacct gcacctcctg aacacggaga cgtcgcagtt cttcggcgag atggcgtacc ttaacgacgc cgagtcggag atgttgccag tggcgaacac gccagtgtac tccacccttg cccagtgccc ggcaacagac gtgatcggcg cggtggtagt ggccaaaggt cggctagccc tctttcaccc tctcatctcc ggctgcattg ccgccacgcc ggagcggtcc attgtaggca gtagagcctg cgtgcagttt agccgggacg gccatcggat cgccatcggc gacgaccacc gcattgtttt actcgatcga cgcagctttt tcggggcacc gctggccgtg ctcgagaaca agcatgtctt taccacgtcg acgtcgctga cgagatgcaa agggctcgag tttagcgccg atgcgaatcg cctgctgctc acatcgtcga gtggtgaagt tgtcgtatac gactggctgc gcggcgagac ggtggcgaac tacttccacg gcgacgcgag gcgacatttt gtcgggtctg ccgacgccat tggcgcgcag cacgtcttcc cttaccagtc agcctcgccc attctgcagc tcacctcgtc catgaccgga ggccggcatc tgcttgtgta cgccggatgc ccgtccaact cggcagcctc cacccgacaa ggacgacttc tgtatgagct gcagtcgaag gacagcgatg tgccggtcgc tatcgccgtg aacccacgac tacagctggc ggctactgca gcgcggagta taacctggtg ggcttttcac aattctgttg tggaggcgta cgcgacactg acggaggcgg gcatgacggc agccgactaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca actccgcctc cccgagccct cccctcacct ccagcggtgg agcagctggc tcggctgctg tggacggagt accgatagag gtggcgtcca tgttctacac aggcatcaac gagccgcggc ccaccgcgat gaacttctcc ttcgacggcc ttcacttcgt gtcggcgcac acggacgacg ccgtccggtt catcgacgtg ggcaacatga cgaacacgga cgtgattatc tgcgacgcgt ttggcgtgca caacgtccgt tacacccact ccagcagtgt cgtatgtgtc gcgccacggt ttcacctgga tggccacctg cacctcctga acacggagac gtcgcagttc ttcggcgaga tggcgtacct taacgacgcc gagtcggaga tgttgccagt ggcgaacacg ccagtgtact ccacccttgc ccagtgcccg gcaacagacg tgatcggcgc ggtggtagtg gccaaaggtc ggctagccct ctttcaccct ctcatctccg gctgcattgc cgccacgccg gagcggtcca ttgtaggcag tagagcctgc gtgcagttta gccgggacgg ccatcggatc gccatcggcg acgaccaccg cattgtttta ctcgatcgac gcagcttttt cggggcaccg ctggccgtgc tcgagaacaa gcatgtcttt accacgtcga cgtcgctgac gagatgcaaa gggctcgagt ttagcgccga tgcgaatcgc ctgctgctca catcgtcgag tggtgaagtt gtcgtatacg actggctgcg cggcgagacg gtggcgaact acttccacgg cgacgcgagg cgacattttg tcgggtctgc cgacgccatt ggcgcgcagc acgtcttccc ttaccagtca gcctcgccca ttctgcagct cacctcgtcc atgaccggag gccggcatct gcttgtgtac gccggatgcc cgtccaactc ggcagcctcc acccgacaag gacgacttct gtatgagctg cagtcgaagg acagcgatgt gccggtcgct atcgccgtga acccacgact acagctggcg gctactgcag cgcggagtat aacctggtgg gcttttcaca attctgttgt ggaggcgtac gcgacactga cggaggcggg catgacggca gccgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LemaA.19526.a.B2.GE41475

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG VPIEVASMFY TGINEPRPTA MNFSFDGLHF VSAHTDDAVR FIDVGNMTNT DVIICDAFGV HNVRYTHSSS VVCVAPRFHL DGHLHLLNTE TSQFFGEMAY LNDAESEMLP VANTPVYSTL AQCPATDVIG AVVVAKGRLA LFHPLISGCI AATPERSIVG SRACVQFSRD GHRIAIGDDH RIVLLDRRSF FGAPLAVLEN KHVFTTSTSL TRCKGLEFSA DANRLLLTSS SGEVVVYDWL RGETVANYFH GDARRHFVGS ADAIGAQHVF PYQSASPILQ LTSSMTGGRH LLVYAGCPSN SAASTRQGRL LYELQSKDSD VPVAIAVNPR LQLAATAARS ITWWAFHNSV VEAYATLT

Validated NT Sequence

atggctcacc accaccacca ccatatggga gtaccgatag aggtggcgtc catgttctac acaggcatca acgagccgcg gcccaccgcg atgaacttct ccttcgacgg ccttcacttc gtgtcggcgc acacggacga cgccgtccgg ttcatcgacg tgggcaacat gacgaacacg gacgtgatta tctgcgacgc gtttggcgtg cacaacgtcc gttacaccca ctccagcagt gtcgtatgtg tcgcgccacg gtttcacctg gatggccacc tgcacctcct gaacacggag acgtcgcagt tcttcggcga gatggcgtac cttaacgacg ccgagtcgga gatgttgcca gtggcgaaca cgccagtgta ctccaccctt gcccagtgcc cggcaacaga cgtgatcggc gcggtggtag tggccaaagg tcggctagcc ctctttcacc ctctcatctc cggctgcatt gccgccacgc cggagcggtc cattgtaggc agtagagcct gcgtgcagtt tagccgggac ggccatcgga tcgccatcgg cgacgaccac cgcattgttt tactcgatcg acgcagcttt ttcggggcac cgctggccgt gctcgagaac aagcatgtct ttaccacgtc gacgtcgctg acgagatgca aagggctcga gtttagcgcc gatgcgaatc gcctgctgct cacatcgtcg agtggtgaag ttgtcgtata cgactggctg cgcggcgaga cggtggcgaa ctacttccac ggcgacgcga ggcgacattt tgtcgggtct gccgacgcca ttggcgcgca gcacgtcttc ccttaccagt cagcctcgcc cattctgcag ctcacctcgt ccatgaccgg aggccggcat ctgcttgtgt acgccggatg cccgtccaac tcggcagcct ccacccgaca aggacgactt ctgtatgagc tgcagtcgaa ggacagcgat gtgccggtcg ctatcgccgt gaacccacga ctacagctgg cggctactgc agcgcggagt ataacctggt gggcttttca caattctgtt gtggaggcgt acgcgacact gacgga

Expected Protein Sequence

MAHHHHHHGV PIEVASMFYT GINEPRPTAM NFSFDGLHFV SAHTDDAVRF IDVGNMTNTD VIICDAFGVH NVRYTHSSSV VCVAPRFHLD GHLHLLNTET SQFFGEMAYL NDAESEMLPV ANTPVYSTLA QCPATDVIGA VVVAKGRLAL FHPLISGCIA ATPERSIVGS RACVQFSRDG HRIAIGDDHR IVLLDRRSFF GAPLAVLENK HVFTTSTSLT RCKGLEFSAD ANRLLLTSSS GEVVVYDWLR GETVANYFHG DARRHFVGSA DAIGAQHVFP YQSASPILQL TSSMTGGRHL LVYAGCPSNS AASTRQGRLL YELQSKDSDV PVAIAVNPRL QLAATAARSI TWWAFHNSVV EAYATLTEAG MTAAD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggagtac cgatagaggt ggcgtccatg ttctacacag gcatcaacga gccgcggccc accgcgatga acttctcctt cgacggcctt cacttcgtgt cggcgcacac ggacgacgcc gtccggttca tcgacgtggg caacatgacg aacacggacg tgattatctg cgacgcgttt ggcgtgcaca acgtccgtta cacccactcc agcagtgtcg tatgtgtcgc gccacggttt cacctggatg gccacctgca cctcctgaac acggagacgt cgcagttctt cggcgagatg gcgtacctta acgacgccga gtcggagatg ttgccagtgg cgaacacgcc agtgtactcc acccttgccc agtgcccggc aacagacgtg atcggcgcgg tggtagtggc caaaggtcgg ctagccctct ttcaccctct catctccggc tgcattgccg ccacgccgga gcggtccatt gtaggcagta gagcctgcgt gcagtttagc cgggacggcc atcggatcgc catcggcgac gaccaccgca ttgttttact cgatcgacgc agctttttcg gggcaccgct ggccgtgctc gagaacaagc atgtctttac cacgtcgacg tcgctgacga gatgcaaagg gctcgagttt agcgccgatg cgaatcgcct gctgctcaca tcgtcgagtg gtgaagttgt cgtatacgac tggctgcgcg gcgagacggt ggcgaactac ttccacggcg acgcgaggcg acattttgtc gggtctgccg acgccattgg cgcgcagcac gtcttccctt accagtcagc ctcgcccatt ctgcagctca cctcgtccat gaccggaggc cggcatctgc ttgtgtacgc cggatgcccg tccaactcgg cagcctccac ccgacaagga cgacttctgt atgagctgca gtcgaaggac agcgatgtgc cggtcgctat cgccgtgaac ccacgactac agctggcggc tactgcagcg cggagtataa cctggtgggc ttttcacaat tctgttgtgg aggcgtacgc gacactgacg gaggcgggca tgacggcagc cgactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat