SSGCID: Target Page

LemaA.19514.a

KREPA4

CENTER ID:	LemaA.19514.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.19514.a.B2.GE40696	truncation1		23	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LmjF.36.0690
RefSeq:	XP_001686641.1
UniProt:	Q4Q207

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRCCRLFAG SGNSTTASSS GAMISLREAT RLAKIAGCFD SANGSSSSLL RRCTDSACAQ GRNVTSSSSS WEEIKRRQHQ VFNERHLTAE NTIAVTVSCV NMAHLSVYVH SVQCGYPASH APGPSGEGVP GKRTITTATL TNQSCAVLLL WVSMGSPELL AVRCALSPGA VETCWRAAPA AASSDTTKAG STIQTAAPSS TLSEKDKLRR IKSWAEELVL HRWVVVSGQL RMEDVYDGDL HKVVAVPVLE VARDHFKGSV LALPPGFL

NT Sequence

atgcggcgtt gttgtcggct ttttgccgga tcagggaaca gcacgacggc gtcttcgagc ggcgccatga tatcgctgcg tgaggcgaca cgtctcgcta agatcgctgg ctgctttgac tctgctaatg ggtcgtcttc gtctttgctg cgtcgctgca ccgattccgc atgtgcgcag ggtcgaaacg tcaccagctc atcatcctcc tgggaggaaa tcaagcggcg gcaacatcag gtgttcaacg agcggcactt aaccgccgag aacaccattg ctgtcacggt gtcgtgtgtg aacatggcgc atctctccgt atacgtgcac tcagtgcaat gcgggtatcc ggcctctcac gcgccaggac cgtctgggga gggcgtgcct ggcaagcgca ccatcaccac tgcaacgttg acgaatcaat cttgcgccgt gctgctcctg tgggtgtcga tggggtcgcc tgagttgttg gccgtgcggt gcgcgctgtc tcccggtgct gtggaaacgt gctggcgtgc ggctccagcc gcggcgagca gcgacaccac taaggctggc agcacgatcc agactgctgc gccatcctcg acgctgtcgg agaaggacaa gctgcgccgc ataaagtcct gggccgagga gctggtcctc catcgatggg tggtggtcag cggccagctg cgcatggagg acgtgtacga cggtgatttg cataaagtag tggcagtgcc ggtgctggag gtggcaaggg accactttaa gggtagcgtg cttgcactgc cgccagggtt tctatga

Details for LemaA.19514.a.B2.GE40696

HARVESTED ON:	data unavailable
SEQUENCED ON:	11/8/2016
EXPECTED MW:	27kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI SLREATRLAK IAGCFDSANG SSSSLLRRCT DSACAQGRNV TSSSSSWEEI KRRQHQVFNE RHLTAENTIA VTVSCVNMAH LSVYVHSVQC GYPASHAPGP SGEGVPGKRT ITTATLTNQS CAVLLLWVSM GSPELLAVRC ALSPGAVETC WRAAPAAASS DTTKAGSTIQ TAAPSSTLSE KDKLRRIKSW AEELVLHRWV VVSGQLRMED VYDGDLHKVV AVPVLEVARD HFKGSVLALP PGFL

Validated NT Sequence

atggctcacc accaccacca ccatatgata tcgctgcgtg aggcgacacg tctcgctaag atcgctggct gctttgactc tgctaatggg tcgtcttcgt ctttgctgcg tcgctgcacc gattccgcat gtgcgcaggg tcgaaacgtc accagctcat catcctcctg ggaggaaatc aagcggcggc aacatcaggt gttcaacgag cggcacttaa ccgccgagaa caccattgct gtcacggtgt cgtgtgtgaa catggcgcat ctctccgtat acgtgcactc agtgcaatgc gggtatccgg cctctcacgc gccaggaccg tctggggagg gcgtgcctgg caagcgcacc atcaccactg caacgttgac gaatcaatct tgcgccgtgc tgctcctgtg ggtgtcgatg gggtcgcctg agttgttggc cgtgcggtgc gcgctgtctc ccggtgctgt ggaaacgtgc tggcgtgcgg ctccagccgc ggcgagcagc gacaccacta aggctggcag cacgatccag actgctgcgc catcctcgac gctgtcggag aaggacaagc tgcgccgcat aaagtcctgg gccgaggagc tggtcctcca tcgatgggtg gtggtcagcg gccagctgcg catggaggac gtgtacgacg gtgatttgca taaagtagtg gcagtgccgg tgctggaggt ggcaagggac cactttaagg gtagcgtgct tgcactgccg ccagggtttc tataagtgag taagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat cgctgcgtga ggcgacacgt ctcgctaaga tcgctggctg ctttgactct gctaatgggt cgtcttcgtc tttgctgcgt cgctgcaccg attccgcatg tgcgcagggt cgaaacgtca ccagctcatc atcctcctgg gaggaaatca agcggcggca acatcaggtg ttcaacgagc ggcacttaac cgccgagaac accattgctg tcacggtgtc gtgtgtgaac atggcgcatc tctccgtata cgtgcactca gtgcaatgcg ggtatccggc ctctcacgcg ccaggaccgt ctggggaggg cgtgcctggc aagcgcacca tcaccactgc aacgttgacg aatcaatctt gcgccgtgct gctcctgtgg gtgtcgatgg ggtcgcctga gttgttggcc gtgcggtgcg cgctgtctcc cggtgctgtg gaaacgtgct ggcgtgcggc tccagccgcg gcgagcagcg acaccactaa ggctggcagc acgatccaga ctgctgcgcc atcctcgacg ctgtcggaga aggacaagct gcgccgcata aagtcctggg ccgaggagct ggtcctccat cgatgggtgg tggtcagcgg ccagctgcgc atggaggacg tgtacgacgg tgatttgcat aaagtagtgg cagtgccggt gctggaggtg gcaagggacc actttaaggg tagcgtgctt gcactgccgc cagggtttct atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat