LemaA.19513.a

KREN1

CENTER ID: LemaA.19513.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19513.a.B2.GE40973 RNase III-like catalytic-like domain 259 546
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LmjF.20.0900
RefSeq: XP_001682842.1
UniProt: Q4QCV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTSSLHLSY TQAMHSSATH RLAATRAPTT TLLCTAHAVN RLPTTCTIPS RDWGSGGGTL FHPTRALRAL KPVTVAHSDT FGSFRQANRA AAGTWWRSST ETASRVKGGR LGRVKDPFQG VFLQLQGCTE VEMTRRSPTP PPDFPLDSLP IYEPHDHNGP PRTKIGFHLP MRSLPASLAL DFFFPGAGSN DSLQAANASS SSSRTHSSGG SANSDLGARY EEADNGAGSG HQQHTSRLDR AAHGTKGLSA AGAGSVTGDT STAFCRLCRE SFDNDGTLRS HITVADRARH VSHPVREVAL ETLTLLAVRG YPIDNIMTVW ADILFNCPLF PRIRSMTDPR WSIEKRAAQI GIILRALKRV GVLDVALATA EPTDVAGSLN SRYRQRRVAF ERLEYIGDNC WGNNISNRIL LLYPDQQWLY SERCSTFTLV RDACEMNVNL DFVFDTLELW HLLSTTAHSR LGSGKVKADL VEGIFGELHL HLYGMVPKLQ DDVEYVETNG AREVQLVALV EHCLTELYDL IVLKHARDLV TSAIPLAKEL AAKRIWARTR PFVLPQKRPN TGRARGKTAM SNGAPHASAF PSAVPTVGRV ATGGYTASFP APHTGVATAN ASVATLEDLF DVSPSSLQQQ QQHDLSACGA ASGTIESRAD GRTEVAVVSN SGKGSRHNTK ERAEEAAAVA RGRAGVMPIS PLFPTPLSLT LSASIGLGST RVLPALPRLF TAPHVYPERV PNPLRLLPLS AIPSMTYCHH TCSDVFARMK MSYERLGLLH EGSSHMHYVV SPTPPRWAVL IATLVPQLVG CAPLPVKPTG DTKGASAEAR RLLASMDQGG LYCRDALYNL AVLPSSPAAA SRPHATPPPM STAWALQNAG LPALSATPSK TREDDDDASS DKSGRSGCTF APSRYVPPGI RAPPAGVVTD RNLCRGVFAF LAVGVADAVA EAERLTRTDT ASVSPATPAG PTTGARSSGE CPAQHSAEDN SPKASATAYA QRMKAAMDYW RRRSQTSLQS LSAASGEASL PR
NT Sequence
atgcccacat catcgttaca tctcagctac acgcaggcga tgcattcctc cgcaacgcac agactcgcag cgacaagggc accaaccacc acgctcctct gcaccgcaca tgctgtaaac cgcctcccca caacgtgcac gattcccagc cgcgactggg gttcaggcgg cggtacgctc ttccacccaa cgcgagcact gagggctctc aaaccagtca ccgtggccca ctccgacacg ttcggctcct ttcggcaggc caacagagca gcggcgggga cgtggtggcg cagctccacc gaaacggcct cccgtgtgaa gggcggaagg cttggcaggg tgaaggatcc gtttcagggc gtctttttgc aactgcaagg ctgcacagag gtggagatga cgcggcgaag ccccactcca ccaccggact tcccgctgga cagtctaccc atctacgaac cccatgacca taacgggcca ccgcgcacca aaatcggctt ccaccttccc atgcgctcgc tgccggcgtc tctagccctc gacttttttt tccctggtgc aggctctaac gatagcctgc aagcagccaa cgcctccagc agcagcagta ggacccatag cagtgggggg tctgcaaaca gtgaccttgg cgcgcgctac gaagaagccg acaacggtgc cggcagtggg catcagcagc acacctcgag gctcgaccgc gcggcgcacg gtacgaaagg actctccgct gcgggcgctg ggagtgtcac tggcgacaca tccacggcgt tctgccgtct gtgtcgcgaa agcttcgata atgatggaac actacggtca cacattacgg tcgcagaccg ggcgcggcac gtctctcacc cggtgcgcga ggtggcgctt gaaacgctga cactgctggc cgtgcgcgga taccccatcg acaatataat gacggtgtgg gccgacatcc tgttcaactg tccactgttt ccgcgcattc gcagcatgac agacccgcgg tggtcgatcg agaagcgggc tgctcaaatt ggcattatcc tgcgggcttt aaagcgggtt ggggtgcttg atgtcgccct tgcgacggca gagccgacgg acgtggcggg ctcgctcaac tcgcgctacc ggcaacgccg cgtcgccttt gaacgactgg agtacattgg cgacaactgc tggggcaaca atatttcgaa tcggatcctg ttgctgtacc ctgatcagca gtggctgtat agcgagcgct gcagcacgtt cacgctggtg cgtgatgcat gtgagatgaa cgtcaatctc gactttgtct tcgacacact ggagctgtgg cacctcttgt cgaccacggc acactcgcga ctagggagtg gaaaggtgaa ggccgacctt gtggaaggga tctttgggga gctgcacctg cacctctacg gtatggtgcc taagctgcag gatgatgtgg agtacgtgga gacgaacggc gctagagagg tgcagctggt cgcactcgtg gagcactgcc tgacagagct atacgacctc atcgtgctga agcacgcacg cgacctcgtc acatcagcca tacctctcgc gaaggagctg gcggcgaagc gaatatgggc gcgaacgcgc ccttttgtgc tgccgcagaa gcgtcccaac actggccggg ctcgaggcaa gactgcgatg agcaacgggg cgccgcatgc atcagccttt ccatcggctg ttccgacggt cggacgcgtt gccaccggcg ggtatacagc gtcctttccg gcaccacaca cgggagtggc gaccgcaaat gcgtctgtgg ctacgctgga ggacctcttc gatgtgtcac cttcgtcctt gcagcagcag cagcagcacg atttgtccgc atgcggggcg gcgagtggta ccatcgagtc gcgcgctgac gggcgcacgg aggtagccgt ggtaagcaac agcggtaaag gtagcaggca taacacgaaa gagcgggccg aagaagcggc tgcagtggcg agggggcgag ccggcgtgat gccaatctct cctttattcc ccaccccctt atccctcacg ctgtcggcct ccattggtct gggtagcacg cgtgtcctgc cggcgttgcc gcgtctcttt accgctccac acgtgtaccc cgaacgcgtg cccaacccgc tgcgcctcct gccgctctcg gccatcccaa gcatgaccta ctgtcaccac acgtgctcgg acgtttttgc gaggatgaag atgagttacg agcgccttgg tctgcttcat gaaggttcga gccacatgca ctatgtggta agccctactc cgccgaggtg ggcggtgctg atcgccacac ttgtgccgca gctcgtggga tgcgcgccgc tgcctgtcaa gccgaccggt gacacaaaag gggcgtcggc cgaagcgcgt cggcttttag cgtccatgga tcagggcggc ctgtactgcc gggacgccct ctacaatctc gcagtgttgc cgtcctcgcc tgctgccgcc tcgcgacccc acgcaacgcc gccgccgatg agcactgcgt gggcgctgca gaatgccggc ttgcccgccc tctctgccac gccgagcaag acaagagagg acgacgacga cgcttcctcg gacaaaagcg ggcgcagcgg gtgcaccttc gctccctccc gctacgtccc gcccggtatt cgagcgccgc cggctggcgt ggtgacagac cgtaacctct gccgcggcgt ctttgccttc ctggctgtcg gtgtggctga cgccgtggca gaggctgagc gactcacacg cacggacacg gcgtcggtca gtcccgctac cccggcgggc cccaccacag gtgcgcggtc ctctggtgag tgcccggcgc agcactcggc agaagacaac tcgcctaaag cgtcggccac tgcgtacgcg cagcggatga aggcggcaat ggactactgg cggcgccgct cgcagacgtc cctccagagc ttatcggccg catccggtga ggcgtcactg cctcgctag
Details for LemaA.19513.a.B2.GE40973
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD TSTAFCRLCR ESFDNDGTLR SHITVADRAR HVSHPVREVA LETLTLLAVR GYPIDNIMTV WADILFNCPL FPRIRSMTDP RWSIEKRAAQ IGIILRALKR VGVLDVALAT AEPTDVAGSL NSRYRQRRVA FERLEYIGDN CWGNNISNRI LLLYPDQQWL YSERCSTFTL VRDACEMNVN LDFVFDTLEL WHLLSTTAHS RLGSGKVKAD LVEGIFGELH LHLYGMVPKL QDDVEYVETN GAREVQLVAL VEHCLTELYD LIVLKHARDL VTSAIPLAKE LAAKRIW
Validated NT Sequence
ttactcactt accatattcg cttcgccgcc agttccttcg cgagaggtat ggctgatgtg acgaggtcgc gtgcgtgctt cagcacgatg aggtcgtata gctctgtcag gcagtgctcc acgagtgcga ccagctgcac ctctctagcg ccgttcgtct ccacgtactc cacatcatcc tgcagcttag gcaccatacc gtagaggtgc aggtgcagct ccccaaagat cccttccaca aggtcggcct tcacctttcc actccctagt cgcgagtgtg ccgtggtcga caagaggtgc cacagctcca gtgtgtcgaa gacaaagtcg agattgacgt tcatctcaca tgcatcacgc accagcgtga acgtgctgca gcgctcgcta tacagccact gctgatcagg gtacagcaac aggatccgat tcgaaatatt gttgccccag cagttgtcgc caatgtactc cagtcgttca aaggcgacgc ggcgttgccg gtagcgcgag ttgagcgagc ccgccacgtc cgtcggctct gccgtcgcaa gggcgacatc aagcacccca acccgcttta aagcccgcag gataatgcca atttgagcag cccgcttctc gatcgaccac cgcgggtctg tcatgctgcg aatgcgcgga aacagtggac agttgaacag gatgtcggcc cacaccgtca ttatattgtc gatggggtat ccgcgcacgg ccagcagtgt cagcgtttca agcgccacct cgcgcaccgg gtgagagacg tgccgcgccc ggtctgcgac cgtaatgtgt gaccgtagtg ttccatcatt atcgaagctt tcgcgacaca gacggcagaa cgccgtggat gtgtccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHDT STAFCRLCRE SFDNDGTLRS HITVADRARH VSHPVREVAL ETLTLLAVRG YPIDNIMTVW ADILFNCPLF PRIRSMTDPR WSIEKRAAQI GIILRALKRV GVLDVALATA EPTDVAGSLN SRYRQRRVAF ERLEYIGDNC WGNNISNRIL LLYPDQQWLY SERCSTFTLV RDACEMNVNL DFVFDTLELW HLLSTTAHSR LGSGKVKADL VEGIFGELHL HLYGMVPKLQ DDVEYVETNG AREVQLVALV EHCLTELYDL IVLKHARDLV TSAIPLAKEL AAKRIW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacacat ccacggcgtt ctgccgtctg tgtcgcgaaa gcttcgataa tgatggaaca ctacggtcac acattacggt cgcagaccgg gcgcggcacg tctctcaccc ggtgcgcgag gtggcgcttg aaacgctgac actgctggcc gtgcgcggat accccatcga caatataatg acggtgtggg ccgacatcct gttcaactgt ccactgtttc cgcgcattcg cagcatgaca gacccgcggt ggtcgatcga gaagcgggct gctcaaattg gcattatcct gcgggcttta aagcgggttg gggtgcttga tgtcgccctt gcgacggcag agccgacgga cgtggcgggc tcgctcaact cgcgctaccg gcaacgccgc gtcgcctttg aacgactgga gtacattggc gacaactgct ggggcaacaa tatttcgaat cggatcctgt tgctgtaccc tgatcagcag tggctgtata gcgagcgctg cagcacgttc acgctggtgc gtgatgcatg tgagatgaac gtcaatctcg actttgtctt cgacacactg gagctgtggc acctcttgtc gaccacggca cactcgcgac tagggagtgg aaaggtgaag gccgaccttg tggaagggat ctttggggag ctgcacctgc acctctacgg tatggtgcct aagctgcagg atgatgtgga gtacgtggag acgaacggcg ctagagaggt gcagctggtc gcactcgtgg agcactgcct gacagagcta tacgacctca tcgtgctgaa gcacgcacgc gacctcgtca catcagccat acctctcgcg aaggagctgg cggcgaagcg aatatggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at