SSGCID: Target Page

LemaA.19511.a

KREN3

CENTER ID:	LemaA.19511.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.19511.a.B1.GE40870	full length		1	631
LemaA.19511.a.B2.GE40871	RNase III-like catalytic-like domain		251	449

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LmjF.36.0920
RefSeq:	XP_001686664.1
UniProt:	Q4Q1Y4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLRRTSAWR AVCHIPDALR THRRDLVSVT CSSSSRPPLR EVTKLFASPM VQEWWVSAAQ EAVISRKATS RAERIVTEAD DPRSGTSHFY DSDARLCLVC NARLEGSYSA HSHSVDHKPR VALLKRTIGM ILTFLEQAGS SPSLPPPPES DSPYSLPGSK RFLVSVSTHT GTPAAPAPSC FHALYAESPL FAEDGVPSRA LLYHRPCTLR EFDLVDVIMR RWWNTLHNPP PRRGSISFDR LLSLSSAELQ MRRWRVRYLL HFLKSRGVLR SCLTLRSDQV NAAPSPNERI HRGEAFERLE MVGDFFFKSL TPDRMHTVFP ADEGGLTAEL QCFERALDSN HGLLAIYDYL GLDDIVGCFL ANNKAKADVV EAVIGELKVL LWSTEVVWGM EYYAVPGERA THVYLRALVA HTIAELGHTV LMWQLESTLR NCRDFLLKHT VEEYLGAEKG NERRASRGGD GCDVPADLPK YSPLPPLLAW NRRRLGVSMR RGEVPLASKQ SRSSIPPSPQ DPLRRLAPTA SCIPEAIRDH YAVGRKKWSS YACKTAIQAL CASLTQTALQ HDSGHTADAL SLNSSSASLP LSPEPQPSPT TPAYACVPRS VLRALVTANC VVIRNSAPPL ARSTSTRRVA G

NT Sequence

atgcgtcttc gccgcacttc tgcgtggcga gcggtttgcc atatccctga tgccttgcgc acgcatcgtc gtgacttggt ttcggtaaca tgttcatcgt catcgaggcc accgctacgt gaggtcacga agctttttgc cagccccatg gtgcaggagt ggtgggtgag cgctgctcaa gaggcggtca tctcccggaa agccaccagt cgggcagagc gcattgtgac ggaggccgac gatccgcgct ccggtacgag tcacttctac gatagtgatg ccagactctg cctcgtgtgc aacgcgcgtc tggagggcag ctacagcgcg cacagccaca gcgtcgacca caaaccgcgc gttgcacttt tgaagcgcac aatcggtatg attctcacat ttctcgagca ggccggctca tcaccgtcgc tgccgccgcc tccggagtcg gactcgccgt actcgttgcc tggcagcaaa cgcttcttag tgtcagtgtc aactcacaca ggaacacctg ccgccccagc cccttcttgc ttccacgcct tgtatgccga gtcacctctg tttgctgaag acggcgtgcc aagtcgcgcg ctcctctatc accgcccgtg cacgctgcgc gagtttgacc tggtagacgt gatcatgcgg cgctggtgga acactttgca taatccacca cctaggcggg gtagtatttc ctttgaccgc ctcctctcgc tttcctcggc tgagctgcag atgcggcgat ggcgtgtgcg ttacctactc cactttctca agtcgagagg ggttctccgc tcttgcctaa cactgaggtc cgaccaagtg aatgcggcgc cgtcccccaa cgagcgtatt caccgtggtg aggcctttga gcggctcgaa atggtcggcg actttttctt caagagcctc acccccgacc gaatgcacac cgtcttcccg gctgatgagg gtggacttac agctgaactc cagtgctttg agcgggcctt ggattcgaat cacggtctgc tggccatcta tgactatctc ggtctggatg acatagtcgg ctgctttttg gccaacaaca aggcaaaggc cgacgttgta gaggccgtca ttggtgagct gaaggttctg ttgtggtcga ctgaggtggt gtggggcatg gagtactacg ccgtacctgg ggagcgcgcc acgcatgtct acctgcgagc gttggtggcg cacaccatcg ccgagctggg gcacacggtg ctgatgtggc agctggagag tacactacgc aattgtcgcg acttccttct caagcacacc gtggaggaat acttgggcgc cgagaaaggg aacgagcgtc gtgcaagcag gggaggtgac gggtgcgacg tccccgcgga ccttccaaag tactcaccgc tgccgccgtt gctcgcgtgg aatcggcgcc gcctgggcgt gtcgatgcgg aggggtgagg tgccgctcgc aagcaaacag tctcgaagca gcataccgcc gtctcctcaa gacccgcttc gcagactcgc cccgactgcg agctgcattc cggaggctat tcgggatcac tacgccgtcg gtcgcaaaaa gtggagctca tatgcctgta aaacagccat tcaagccctc tgcgcctccc tcacacaaac tgcgctgcag catgacagcg gtcacaccgc tgacgctcta tcgttgaact catcgtcggc atccttgccc ctctctccag aaccgcagcc gtccccaact acgccggcgt atgcgtgcgt ccctcgcagt gtgttacggg ctctggtgac tgcgaattgc gtggtgattc gtaattcggc cccgcccctc gctagaagca cgtcgactag gagggtggct gggtag

Details for LemaA.19511.a.B1.GE40870

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR LRRTSAWRAV CHIPDALRTH RRDLVSVTCS SSSRPPLREV TKLFASPMVQ EWWVSAAQEA VISRKATSRA ERIVTEADDP RSGTSHFYDS DARLCLVCNA RLEGSYSAHS HSVDHKPRVA LLKRTIGMIL TFLEQAGSSP SLPPPPESDS PYSLPGSKRF LVSVSTHTGT PAAPAPSCFH ALYAESPLFA EDGVPSRALL YHRPCTLREF DLVDVIMRRW WNTLHNPPPR RGSISFDRLL SLSSAELQMR RWRVRYLLHF LKSRGVLRSC LTLRSDQVNA APSPNERIHR GEAFERLEMV GDFFFKSLTP DRMHTVFPAD EGGLTAELQC FERALDSNHG LLAIYDYLGL DDIVGCFLAN NKAKADVVEA VIGELKVLLW STEVVWGMEY YAVPGERATH VYLRALVAHT IAELGHTVLM WQLESTLRNC RDFLLKHTVE EYLGAEKGNE RRASRGGDGC DVPADLPKYS PLPPLLAWNR RRLGVSMRRG EVPLASKQSR SSIPPSPQDP LRRLAPTASC IPEAIRDHYA VGRKKWSSYA CKTAIQALCA SLTQTALQHD SGHTADALSL NSSSASLPLS PEPQPSPTTP AYACVPRSVL RALVTANCVV IRNSAPPLAR STSTRRVAG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtc ttcgccgcac ttctgcgtgg cgagcggttt gccatatccc tgatgccttg cgcacgcatc gtcgtgactt ggtttcggta acatgttcat cgtcatcgag gccaccgcta cgtgaggtca cgaagctttt tgccagcccc atggtgcagg agtggtgggt gagcgctgct caagaggcgg tcatctcccg gaaagccacc agtcgggcag agcgcattgt gacggaggcc gacgatccgc gctccggtac gagtcacttc tacgatagtg atgccagact ctgcctcgtg tgcaacgcgc gtctggaggg cagctacagc gcgcacagcc acagcgtcga ccacaaaccg cgcgttgcac ttttgaagcg cacaatcggt atgattctca catttctcga gcaggccggc tcatcaccgt cgctgccgcc gcctccggag tcggactcgc cgtactcgtt gcctggcagc aaacgcttct tagtgtcagt gtcaactcac acaggaacac ctgccgcccc agccccttct tgcttccacg ccttgtatgc cgagtcacct ctgtttgctg aagacggcgt gccaagtcgc gcgctcctct atcaccgccc gtgcacgctg cgcgagtttg acctggtaga cgtgatcatg cggcgctggt ggaacacttt gcataatcca ccacctaggc ggggtagtat ttcctttgac cgcctcctct cgctttcctc ggctgagctg cagatgcggc gatggcgtgt gcgttaccta ctccactttc tcaagtcgag aggggttctc cgctcttgcc taacactgag gtccgaccaa gtgaatgcgg cgccgtcccc caacgagcgt attcaccgtg gtgaggcctt tgagcggctc gaaatggtcg gcgacttttt cttcaagagc ctcacccccg accgaatgca caccgtcttc ccggctgatg agggtggact tacagctgaa ctccagtgct ttgagcgggc cttggattcg aatcacggtc tgctggccat ctatgactat ctcggtctgg atgacatagt cggctgcttt ttggccaaca acaaggcaaa ggccgacgtt gtagaggccg tcattggtga gctgaaggtt ctgttgtggt cgactgaggt ggtgtggggc atggagtact acgccgtacc tggggagcgc gccacgcatg tctacctgcg agcgttggtg gcgcacacca tcgccgagct ggggcacacg gtgctgatgt ggcagctgga gagtacacta cgcaattgtc gcgacttcct tctcaagcac accgtggagg aatacttggg cgccgagaaa gggaacgagc gtcgtgcaag caggggaggt gacgggtgcg acgtccccgc ggaccttcca aagtactcac cgctgccgcc gttgctcgcg tggaatcggc gccgcctggg cgtgtcgatg cggaggggtg aggtgccgct cgcaagcaaa cagtctcgaa gcagcatacc gccgtctcct caagacccgc ttcgcagact cgccccgact gcgagctgca ttccggaggc tattcgggat cactacgccg tcggtcgcaa aaagtggagc tcatatgcct gtaaaacagc cattcaagcc ctctgcgcct ccctcacaca aactgcgctg cagcatgaca gcggtcacac cgctgacgct ctatcgttga actcatcgtc ggcatccttg cccctctctc cagaaccgca gccgtcccca actacgccgg cgtatgcgtg cgtccctcgc agtgtgttac gggctctggt gactgcgaat tgcgtggtga ttcgtaattc ggccccgccc ctcgctagaa gcacgtcgac taggagggtg gctgggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LemaA.19511.a.B2.GE40871

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR RWRVRYLLHF LKSRGVLRSC LTLRSDQVNA APSPNERIHR GEAFERLEMV GDFFFKSLTP DRMHTVFPAD EGGLTAELQC FERALDSNHG LLAIYDYLGL DDIVGCFLAN NKAKADVVEA VIGELKVLLW STEVVWGMEY YAVPGERATH VYLRALVAHT IAELGHTVLM WQLESTLRNC RDFLLKHTVE EYLGAEK

Validated NT Sequence

atcttactca cttatttctc ggcgcccaag tattcctcca cggtgtgctt gagaaggaag tcgcgacaat tgcgtagtgt actctccagc tgccacatca gcaccgtgtg ccccagctcg gcgatggtgt gcgccaccaa cgctcgcagg tagacatgcg tggcgcgctc cccaggtacg gcgtagtact ccatgcccca caccacctca gtcgaccaca acagaacctt cagctcacca atgacggcct ctacaacgtc ggcctttgcc ttgttgttgg ccaaaaagca gccgactatg tcatccagac cgagatagtc atagatggcc agcagaccgt gattcgaatc caaggcccgc tcaaagcact ggagttcagc tgtaagtcca ccctcatcag ccgggaagac ggtgtgcatt cggtcggggg tgaggctctt gaagaaaaag tcgccgacca tttcgagccg ctcaaaggcc tcaccacggt gaatacgctc gttgggggac ggcgccgcat tcacttggtc ggacctcagt gttaggcaag agcggagaac ccctctcgac ttgagaaagt ggagtaggta acgcacacgc catcgccgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggc gatggcgtgt gcgttaccta ctccactttc tcaagtcgag aggggttctc cgctcttgcc taacactgag gtccgaccaa gtgaatgcgg cgccgtcccc caacgagcgt attcaccgtg gtgaggcctt tgagcggctc gaaatggtcg gcgacttttt cttcaagagc ctcacccccg accgaatgca caccgtcttc ccggctgatg agggtggact tacagctgaa ctccagtgct ttgagcgggc cttggattcg aatcacggtc tgctggccat ctatgactat ctcggtctgg atgacatagt cggctgcttt ttggccaaca acaaggcaaa ggccgacgtt gtagaggccg tcattggtga gctgaaggtt ctgttgtggt cgactgaggt ggtgtggggc atggagtact acgccgtacc tggggagcgc gccacgcatg tctacctgcg agcgttggtg gcgcacacca tcgccgagct ggggcacacg gtgctgatgt ggcagctgga gagtacacta cgcaattgtc gcgacttcct tctcaagcac accgtggagg aatacttggg cgccgagaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat