LemaA.19510.a

KREN2

CENTER ID: LemaA.19510.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19510.a.B1.GE40970 full length 1 587
LemaA.19510.a.B2.GE40971 RNase III-like catalytic-like domain 136 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LmjF.36.1050
RefSeq: XP_001686677.1
UniProt: Q6T449

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRCGVKRRL SLRLPVVSIA GLRRRARRAA GTRSGSVALC VSAASIGSST SLASTPGRKA PVYYNFATPV SVLRDAAGSE SVSHRRPRRE APAREGGLLL VAPRTPNPND PLQRLNIFRR EFSDFSSAAK LNPNIMVSVD TSTRRFLEMF CDFDAKRCKL CNETFLQWHI HANGIPHAGR EGMLLELVRP YCGTPDELIE MWWQRLNSCA AFHRIPGLSH DNPHERKRRL LYLLKVLKDR GILVETFNVS DNQSTNSARS WEFERLEFVG DNVVKYVLNN IISCAFPPHE GGTKGKMTCF QFVMDGNDGL ARGYDHLDLQ QLASSPRVVS KFKSDIVETL FAELQMYLWS TQHDIGTCPL VFPFTKDIYT LRALVQHVLY ELGIELFLYH IRHINGMLQR VMRENHLQFV KADSALKPRA GARTGSLGVS TRRGDWSDDD DDYDCPQSYG TPAVSAPTAA LMKVRSQRTH VQGTGAALFF ASTNYDNFKR VVPIGGLLPR PFARQDLSVI PNHMPHLRSD GAMTRRMRRV GNAGWSTLHA TATALTTLES VADGLALSAE TTPRVGSEPV RLSVPRLKDE ELIPELI
NT Sequence
atgaaccgct gtggtgtaaa gaggcgcctt tcactgcggc tgccagtggt gtcaatcgca ggccttcggc ggcgagcgcg aagagcagca ggcacgcgta gcggcagtgt tgcactttgt gtctctgctg cgtctattgg cagtagcacc agcctcgcta gcacccctgg ccggaaagct cccgtgtact acaactttgc cacgccggtg agtgtgctac gtgacgcggc gggtagtgaa agtgtgtctc accgacgccc gcgccgcgag gcgccggctc gagagggagg gctgctactt gtggcgccgc gcacgccgaa cccgaacgac ccgcttcaac ggctgaacat ttttcgacgc gagttttccg atttcagcag cgccgccaag ctcaatccga atatcatggt gagcgtcgac accagcacgc gccgcttcct cgaaatgttc tgtgacttcg atgccaaacg ctgcaagctg tgcaatgaga cgttcttgca gtggcacatc cacgccaacg gcattccgca cgcggggcga gaggggatgc tgctggagct tgttcgcccg tattgtggaa ctccggatga actcatagag atgtggtggc agcggctgaa ctcgtgtgct gcgtttcacc ggattcccgg cttgagccac gacaacccac acgagcgcaa gcggcgtctg ctatatctgt tgaaagtgct gaaagaccgc ggcatccttg tagagacgtt caacgtgagt gacaaccagt ccaccaactc agcgcgctcg tgggagtttg agcgcctcga gttcgtgggc gacaacgtgg tcaagtacgt gttgaacaac attatttcct gtgcattccc acctcacgag ggcggcacaa aaggaaagat gacgtgcttt cagttcgtga tggacggcaa cgatggtttg gcacgcggct acgaccacct cgacctccag cagctcgcct ccagccctag ggtagtcagc aaatttaaga gcgacattgt cgagaccctg ttcgcggagc tgcagatgta cctgtggagc acgcagcacg atattggcac ctgtcctctt gtgtttccat ttacgaagga tatttacacc ctgcgggcgc tggtacagca tgtgctgtac gagctgggta ttgaactctt tctctaccac attcgccaca ttaacggcat gctacaacgc gtgatgcgag agaatcacct gcagttcgta aaagcagatt ccgcgctgaa gcccagggcg ggcgccagga ccgggagctt gggcgtgagc acgcgccgtg gtgactggag cgatgacgac gatgactacg actgtccgca aagctacggt acgccggcgg tgtccgcccc cacggcagcc ctcatgaagg tgcgctcgca gcgcacccac gtgcaaggca ccggtgcggc gctgttcttt gcctccacaa actacgataa cttcaagcgc gtggtaccga tcggcggtct tttgccgcgg ccatttgcga ggcaggattt gtcagtcatc ccaaaccaca tgccgcacct ccggagcgat ggcgcgatga cgcggaggat gaggagggtc gggaatgccg gttggtcgac cctgcacgcc acagcaacag ctctgaccac gttggaaagc gttgccgacg gtctcgccct cagtgccgaa acaacgcctc gtgtgggcag cgagccagta cgcttgtcag ttccgcgtct caaggacgag gagctcattc cagagctgat ttaa
Details for LemaA.19510.a.B1.GE40970
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN RCGVKRRLSL RLPVVSIAGL RRRARRAAGT RSGSVALCVS AASIGSSTSL ASTPGRKAPV YYNFATPVSV LRDAAGSESV SHRRPRREAP AREGGLLLVA PRTPNPNDPL QRLNIFRREF SDFSSAAKLN PNIMVSVDTS TRRFLEMFCD FDAKRCKLCN ETFLQWHIHA NGIPHAGREG MLLELVRPYC GTPDELIEMW WQRLNSCAAF HRIPGLSHDN PHERKRRLLY LLKVLKDRGI LVETFNVSDN QSTNSARSWE FERLEFVGDN VVKYVLNNII SCAFPPHEGG TKGKMTCFQF VMDGNDGLAR GYDHLDLQQL ASSPRVVSKF KSDIVETLFA ELQMYLWSTQ HDIGTCPLVF PFTKDIYTLR ALVQHVLYEL GIELFLYHIR HINGMLQRVM RENHLQFVKA DSALKPRAGA RTGSLGVSTR RGDWSDDDDD YDCPQSYGTP AVSAPTAALM KVRSQRTHVQ GTGAALFFAS TNYDNFKRVV PIGGLLPRPF ARQDLSVIPN HMPHLRSDGA MTRRMRRVGN AGWSTLHATA TALTTLESVA DGLALSAETT PRVGSEPVRL SVPRLKDEEL IPELI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gctgtggtgt aaagaggcgc ctttcactgc ggctgccagt ggtgtcaatc gcaggccttc ggcggcgagc gcgaagagca gcaggcacgc gtagcggcag tgttgcactt tgtgtctctg ctgcgtctat tggcagtagc accagcctcg ctagcacccc tggccggaaa gctcccgtgt actacaactt tgccacgccg gtgagtgtgc tacgtgacgc ggcgggtagt gaaagtgtgt ctcaccgacg cccgcgccgc gaggcgccgg ctcgagaggg agggctgcta cttgtggcgc cgcgcacgcc gaacccgaac gacccgcttc aacggctgaa catttttcga cgcgagtttt ccgatttcag cagcgccgcc aagctcaatc cgaatatcat ggtgagcgtc gacaccagca cgcgccgctt cctcgaaatg ttctgtgact tcgatgccaa acgctgcaag ctgtgcaatg agacgttctt gcagtggcac atccacgcca acggcattcc gcacgcgggg cgagagggga tgctgctgga gcttgttcgc ccgtattgtg gaactccgga tgaactcata gagatgtggt ggcagcggct gaactcgtgt gctgcgtttc accggattcc cggcttgagc cacgacaacc cacacgagcg caagcggcgt ctgctatatc tgttgaaagt gctgaaagac cgcggcatcc ttgtagagac gttcaacgtg agtgacaacc agtccaccaa ctcagcgcgc tcgtgggagt ttgagcgcct cgagttcgtg ggcgacaacg tggtcaagta cgtgttgaac aacattattt cctgtgcatt cccacctcac gagggcggca caaaaggaaa gatgacgtgc tttcagttcg tgatggacgg caacgatggt ttggcacgcg gctacgacca cctcgacctc cagcagctcg cctccagccc tagggtagtc agcaaattta agagcgacat tgtcgagacc ctgttcgcgg agctgcagat gtacctgtgg agcacgcagc acgatattgg cacctgtcct cttgtgtttc catttacgaa ggatatttac accctgcggg cgctggtaca gcatgtgctg tacgagctgg gtattgaact ctttctctac cacattcgcc acattaacgg catgctacaa cgcgtgatgc gagagaatca cctgcagttc gtaaaagcag attccgcgct gaagcccagg gcgggcgcca ggaccgggag cttgggcgtg agcacgcgcc gtggtgactg gagcgatgac gacgatgact acgactgtcc gcaaagctac ggtacgccgg cggtgtccgc ccccacggca gccctcatga aggtgcgctc gcagcgcacc cacgtgcaag gcaccggtgc ggcgctgttc tttgcctcca caaactacga taacttcaag cgcgtggtac cgatcggcgg tcttttgccg cggccatttg cgaggcagga tttgtcagtc atcccaaacc acatgccgca cctccggagc gatggcgcga tgacgcggag gatgaggagg gtcgggaatg ccggttggtc gaccctgcac gccacagcaa cagctctgac cacgttggaa agcgttgccg acggtctcgc cctcagtgcc gaaacaacgc ctcgtgtggg cagcgagcca gtacgcttgt cagttccgcg tctcaaggac gaggagctca ttccagagct gatttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LemaA.19510.a.B2.GE40971
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 34kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV SVDTSTRRFL EMFCDFDAKR CKLCNETFLQ WHIHANGIPH AGREGMLLEL VRPYCGTPDE LIEMWWQRLN SCAAFHRIPG LSHDNPHERK RRLLYLLKVL KDRGILVETF NVSDNQSTNS ARSWEFERLE FVGDNVVKYV LNNIISCAFP PHEGGTKGKM TCFQFVMDGN DGLARGYDHL DLQQLASSPR VVSKFKSDIV ETLFAELQMY LWSTQHDIGT CPLVFPFTKD IYTLRALVQH VLYELGIELF LYHIRHINGM LQRVMRENHL QLVKADSAL
Validated NT Sequence
ttactcactt acagcgcgga atctgctttt actaactgca ggtgattctc tcgcatcacg cgttgtagca tgccgttaat gtggcgaatg tggtagagaa agagttcaat acccagctcg tacagcacat gctgtaccag cgcccgcagg gtgtaaatat ccttcgtaaa tggaaacaca agaggacagg tgccaatatc gtgctgcgtg ctccacaggt acatctgcag ctccgcgaac agggtctcga caatgtcgct cttaaatttg ctgactaccc tagggctgga ggcgagctgc tggaggtcga ggtggtcgta gccgcgtgcc aaaccatcgt tgccgtccat cacgaactga aagcacgtca tctttccttt tgtgccgccc tcgtgaggtg ggaatgcaca ggaaataatg ttgttcaaca cgtacttgac cacgttgtcg cccacgaact cgaggcgctc aaactcccac gagcgcgctg agttggtgga ctggttgtca ctcacgttga acgtctctac aaggatgccg cggtctttca gcactttcaa cagatatagc agacgccgct tgcgctcgtg tgggttgtcg tggctcaagc cgggaatccg gtgaaacgca gcacacgagt tcagccgctg ccaccacatc tctatgagtt catccggagt tccacaatac gggcgaacaa gctccagcag catcccctct cgccccgcgt gcggaatgcc gttggcgtgg atgtgccact gcaagaacgt ctcattgcac agcttgcagc gtttggcatc gaagtcacag aacatttcga ggaagcggcg cgtgctggtg tcgacgctca ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMV SVDTSTRRFL EMFCDFDAKR CKLCNETFLQ WHIHANGIPH AGREGMLLEL VRPYCGTPDE LIEMWWQRLN SCAAFHRIPG LSHDNPHERK RRLLYLLKVL KDRGILVETF NVSDNQSTNS ARSWEFERLE FVGDNVVKYV LNNIISCAFP PHEGGTKGKM TCFQFVMDGN DGLARGYDHL DLQQLASSPR VVSKFKSDIV ETLFAELQMY LWSTQHDIGT CPLVFPFTKD IYTLRALVQH VLYELGIELF LYHIRHINGM LQRVMRENHL QFVKADSAL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga gcgtcgacac cagcacgcgc cgcttcctcg aaatgttctg tgacttcgat gccaaacgct gcaagctgtg caatgagacg ttcttgcagt ggcacatcca cgccaacggc attccgcacg cggggcgaga ggggatgctg ctggagcttg ttcgcccgta ttgtggaact ccggatgaac tcatagagat gtggtggcag cggctgaact cgtgtgctgc gtttcaccgg attcccggct tgagccacga caacccacac gagcgcaagc ggcgtctgct atatctgttg aaagtgctga aagaccgcgg catccttgta gagacgttca acgtgagtga caaccagtcc accaactcag cgcgctcgtg ggagtttgag cgcctcgagt tcgtgggcga caacgtggtc aagtacgtgt tgaacaacat tatttcctgt gcattcccac ctcacgaggg cggcacaaaa ggaaagatga cgtgctttca gttcgtgatg gacggcaacg atggtttggc acgcggctac gaccacctcg acctccagca gctcgcctcc agccctaggg tagtcagcaa atttaagagc gacattgtcg agaccctgtt cgcggagctg cagatgtacc tgtggagcac gcagcacgat attggcacct gtcctcttgt gtttccattt acgaaggata tttacaccct gcgggcgctg gtacagcatg tgctgtacga gctgggtatt gaactctttc tctaccacat tcgccacatt aacggcatgc tacaacgcgt gatgcgagag aatcacctgc agttcgtaaa agcagattcc gcgctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t