SSGCID: Target Page

LemaA.19509.a

KREPA2

CENTER ID:	LemaA.19509.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.19509.a.B2.GE40693	C2H2 zinc finger and OB fold		435	623

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LmjF.36.6930
RefSeq:	XP_001687287.1
UniProt:	Q6T448

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTMKRRIGVL AAAAASRCAL RRLHHVSSRP LHASGAVASA KGASNAALLG AVRNQSAVPD RRFHCSVCKK SFRLEMAAKL HLQQVHSGEG AVEAGAGPGQ GEDQASQTPV GVFRNAPPQA PTVVTPIVPD THERAARREK PAPKPLHQAE REVPGIVMEK MLSVWDDTGV KRMGDQFVHS SMVMRVFAAR PSDSAEPLYG VMNPEGENPF EGGPYAPDVA GSITKDEVKF YNIGIGDAFA LACGESFGPL RPARCPNPFL RKLAKDAAKT APRVGLAEQQ TAPVTPFGQL PMFGQTPSPA TAPAPSAAEE PSTTINSVVS SPFAAGANDS PFAGTTDFPF AGQGLSPFGS VKEPADQAAA EPGIAARSAA SSEAPASPFV AASSALPPSP FAAAPSPFTE SSGAAAPYAA RDAAAVASGA EGHDAAFFSS SAASSVSAPA EVAPAPTHVC TVCKKNFSTY DGLRMHSKAK HGEELPKGRR NGKREVPDLP AFIPSPVDLT MTSPFGSSAQ RSAWTEVELK PYAQSVSNIT VAGRVLDSEV LSDGALLLSL FVRDSAESES EVIPVRCTSA AFRTLGRTLV YGDLVFACGS LRVLPFTDKK TQKRYSSAVV HVGLPTGMIA KLN

NT Sequence

atgacgatga agaggcggat cggtgtcttg gccgctgctg cagcgagccg ctgcgcgctg cgccggctgc accacgtgtc gtctcggccc ttgcacgcgt cgggtgctgt cgcctccgcg aaaggcgcct caaatgccgc tctgttgggg gctgtgcgga atcaaagtgc cgtcccggac cggcgcttcc actgcagcgt ttgcaaaaag tcgtttcgct tagagatggc ggcaaagctg caccttcagc aggtgcacag cggtgagggc gcggtggagg caggcgctgg tcccggacag ggagaggatc aggcgtcgca gaccccggtg ggtgtatttc gtaatgcgcc tccgcaggca ccaaccgtcg ttacaccgat cgtgccagac actcacgagc gcgctgcgcg ccgtgagaag ccggcaccaa agccacttca ccaagccgaa cgcgaggtgc caggtattgt gatggaaaag atgctctctg tatgggatga cacaggggtg aagcggatgg gggatcagtt tgttcacagt agcatggtaa tgcgcgtgtt tgccgctcgc ccgtctgaca gcgccgagcc gttgtacggc gtcatgaatc cagagggtga gaacccgttc gagggtggcc cgtatgcccc cgacgtggct ggttctatca cgaaggacga ggtcaaattt tacaacatcg gtataggtga cgcgtttgcg ctggcgtgtg gcgagtcttt cggtccattg cgtcctgctc ggtgcccaaa cccattcctc cgaaaactag caaaggatgc tgcgaaaacc gccccaaggg tggggttggc agagcagcaa acggcgccgg taacaccgtt cggacagctc cccatgtttg ggcagactcc gtctcctgct acggccccag ctcccagcgc cgccgaggaa ccgtcgacca cgatcaacag cgtcgtctct tcccccttcg ccgccggcgc caacgactcg ccttttgctg ggacgacgga ctttccgttt gctgggcaag ggctctctcc gttcggatcg gtgaaggagc cagcagatca ggccgctgcc gagcccggga ttgccgcgcg atcggcggcc tcgtcggagg cgccggcctc accgttcgtg gctgcatcta gtgctcttcc tccgtccccg ttcgccgcag ctccgagccc tttcaccgaa tcttctgggg cagcagcccc gtatgctgcg agggacgcag ccgcggtggc aagcggcgct gagggtcatg acgccgcctt cttttcctct tccgccgcgt cttccgtgag cgcgccggct gaggtggcgc ccgcaccgac gcatgtgtgt accgtttgca agaagaactt ttcgacgtat gacggccttc gaatgcatag caaggccaag catggggagg agctgccgaa ggggcggcga aacgggaagc gcgaggttcc ggacttacca gctttcattc ccagtccggt ggacctcacg atgacgtcac ccttcggctc gtcggcgcaa cggtcggcgt ggacggaagt ggagctgaaa ccgtacgcgc agtctgtgag taacattact gtggcgggcc gcgtgctgga tagcgaagtt ttgagtgacg gggcgttgct tttgtcgctt ttcgtccgcg actcggccga aagcgagtct gaggtaattc ctgttcgatg cacctcagct gcctttagaa cgcttggcag aacgctcgtg tatggcgatc tcgtgttcgc ctgtggttcg ctgcgcgttt tgccatttac ggataagaaa acacaaaaga gatactcctc ggcagtcgtg cacgtgggtc ttccaactgg catgattgcc aaactgaatt ga

Details for LemaA.19509.a.B2.GE40693

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	21kDa
OBSERVED MW:	21kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHSV SAPAEVAPAP THVCTVCKKN FSTYDGLRMH SKAKHGEELP KGRRNGKREV PDLPAFIPSP VDLTMTSPFG SSAQRSAWTE VELKPYAQSV SNITVAGRVL DSEVLSDGAL LLSLFVRDSA ESESEVIPVR CTSAAFRTLG RTLVYGDLVF ACGSLRVLPF TDKKTQKRYS SAVVHVGLPT GMIAKLN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccgtga gcgcgccggc tgaggtggcg cccgcaccga cgcatgtgtg taccgtttgc aagaagaact tttcgacgta tgacggcctt cgaatgcata gcaaggccaa gcatggggag gagctgccga aggggcggcg aaacgggaag cgcgaggttc cggacttacc agctttcatt cccagtccgg tggacctcac gatgacgtca cccttcggct cgtcggcgca acggtcggcg tggacggaag tggagctgaa accgtacgcg cagtctgtga gtaacattac tgtggcgggc cgcgtgctgg atagcgaagt tttgagtgac ggggcgttgc ttttgtcgct tttcgtccgc gactcggccg aaagcgagtc tgaggtaatt cctgttcgat gcacctcagc tgcctttaga acgcttggca gaacgctcgt gtatggcgat ctcgtgttcg cctgtggttc gctgcgcgtt ttgccattta cggataagaa aacacaaaag agatactcct cggcagtcgt gcacgtgggt cttccaactg gcatgattgc caaactgaat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat