LemaA.19508.a

KREPB4

CENTER ID: LemaA.19508.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19508.a.B1.GE40869 full length 1 568
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LmjF.33.1970
RefSeq: XP_001685958.1
UniProt: Q4Q3Z0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQAQLGRGHA ALLRLYRVVA ALVSTAPIWR HPATAAKGPS YSADVIAPRL SFAGNRRATV FTSSCCRLTP ARCMTTVPES TCAASCESQL CLLCDTPYTS WTEHSREHAH VARSVVCRFF VMPERSESIM QHLERHIALD LKEVDALTDR KVGRRRERLL AGLVHLVEKG VLRDSIPTLP FSTPARGGTD KGRGAGISGV TLGVDADLFL NRVLVGEAFL RREVLDRCAR LMPSLTAMEL NSVVTYLTST RQMARIFDEL SLIELLVTRS GLESAPANVN TATAAATAAG RAAESASVEP LSETAAAPLP DSDASTESPP AHQPCSSTLA ASLPSPPRWS LSREDKVYIM CACLGELHRF HEQERPRSVI SKAAADAIVL NVLAGHGREN LVSELVHETL QRIVDEGTVV WRQHQEDVKR RGRSSEANVA GAARISARRA PRTDSTVSED LCYVRTAPLP SAPSPRPASQ VTLDSAAAEQ LVSSYLPFND VDQLSGAETD AVKATAEAPS RSSPDRTCTT SPECISVLHT SEKDWGPLTA ELRKRAEDWN RLPFVPSTPE LKASKMPR
NT Sequence
atgcaggctc agctcgggag aggacatgcg gcactgctgc ggctgtaccg cgtcgtcgcc gccctggtgt cgacggcgcc gatctggcgc cacccggcga ccgctgccaa aggaccgagt tacagcgcgg atgtgattgc accacgcttg agtttcgccg gaaaccggcg cgccaccgtc ttcacctctt catgctgccg ccttactcct gcgcgatgca tgacgacagt gccggagagc acatgcgcgg catcgtgtga gtcgcagctt tgtctgctgt gcgacacccc ctacacctcc tggacggagc actcccgcga gcatgcgcac gtggcgcgct cggtggtgtg ccgtttcttt gtgatgccag agagaagcga gagcatcatg cagcacctag agcggcacat cgctctggac ctcaaggagg tggatgccct cacagaccgc aaggtagggc ggcgccgcga gcgtctcctc gcgggccttg tgcaccttgt cgagaagggc gtgctgcggg atagcatacc cacgctgccg ttctcaacgc cggcacgcgg gggaactgac aagggaaggg gtgctggcat tagcggcgtg acgcttgggg tcgacgctga cctctttctc aaccgcgtcc tcgtcgggga ggcgttcctg cgccgtgagg tgcttgaccg atgcgcgcgg ctcatgccgt ccctcacagc gatggagctg aacagcgtag tgacgtactt gacgtcgacg cgtcaaatgg ctcgcatctt tgacgagctg tcgctgatag agcttctcgt gacgcgttcg gggttggaaa gcgctcccgc gaacgtgaac accgccaccg ctgctgccac tgccgccgga agagcggcag agtcagcgag cgtagagccg ctgagcgaga cagctgcggc acctttgcca gacagcgacg cgagcacaga gtcgccacct gcgcatcagc cctgctcttc cacactggcc gccagtcttc cctctccccc acgctggagc ctgtcccgcg aggacaaggt gtacatcatg tgcgcctgtc tcggcgagct gcaccgcttt cacgagcagg agcgaccgcg ctctgtcatc tccaaggcag cagcagatgc tattgtgctg aacgtgcttg ccggccacgg gcgcgagaac cttgtgtcgg agttggtgca tgaaacattg cagcgtatcg tggacgaggg gacggtggtc tggcggcagc atcaggagga tgtgaagcgg cgcggtcgtt catctgaagc aaacgtggca ggggcagctc gaatcagcgc gcggcgggca cctcgcacgg acagtaccgt atccgaggac ttgtgctacg tgcgcactgc accgctgccg tcagcgccgt caccgcggcc ggcgtctcag gttacactgg atagcgcagc agcggagcag ctcgtgtctt cttacttgcc cttcaatgat gtcgatcaac ttagcggcgc cgagactgac gctgtgaaag cgacagcaga agcgccgtcg aggtcgagtc cggaccgcac ctgcactact tctcccgagt gcatttcagt gctgcacact tcggagaagg actgggggcc gctcacggcg gagctgcgga agcgtgccga ggattggaat cggctaccct ttgtgcctag cacacccgag ctaaaagcgt cgaagatgcc gcgctaa
Details for LemaA.19508.a.B1.GE40869
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 63kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 35
Validated AA Sequence
MAHHHHHHMQ AQLGRGHAAL LRLYRVVAAL VSTAPIWRHP ATAAKGPSYS ADVIAPRLSF AGNRRATVFT SSCCRLTPAR CMTTVPESTC AASCESQLCL LCDTPYTSWT EHSREHAHVA RSVVCRFFVM PERSESIMQH LERHIALDLK EVDALTDRKV GRRRERLLAG LVHLVEKGVL RDSIPTLPFS TPARGELTRE GVLALAA
Validated NT Sequence
tagctcgggt gtgctaggca caaagggtag ccgattccaa tcctcggcac gcttccgcag ctccgccgtg agcggccccc agtccttctc cgaagtgtgc agcactgaaa tgcactcggg agaagtagtg caggtgcggt ccggactcga cctcgacggc gcttctgctg tcgctttcac agcgtcagtc tcggcgccgc taagttgatc gacatcattg aagggcaagt aagaagacac gagctgctcc gctgctgcgc tatccagtgt aacctgagac gccggccgcg gtgacggcgc tgacggcagc ggtgcagtgc gcacgtagca caagtcctcg gatacggtac tgtccgtgcg aggtgcccgc cgcgcgctga ttcgagctgc ccctgccacg tttgcttcag atgaacgacc gcgccgcttc acatcctcct gatgctgccg ccagaccacc gtcccctcgt ccacgatacg ctgcaatgtt tcatgcacca actccgacac aaggttctcg cgcccgtggc cggcaagcac gttcagcaca atagcatctg ctgctgcctt ggagatgaca gagcgcggtc gctcctgctc gtgaaagcgg tgcagctcgc cgagacaggc gcacatgatg tacaccttgt cctcgcggga caggctccag cgtgggggag agggaagact ggcggccagt gtggaagagc agggctgatg cgcnnntggc gactctgtgc tcgcgtcgct gtctgncnnn ggtgccgcag ctgtctcgct cagcggctct acgctcgctg actctgccgc tcttccgncn gcagtggcag cagcggtggc ggtgttcacg ttcgcgggag cgctttccan nnnnnnaacg cgtcacgaga agctctatca gcgacagctc gtcaaagatg cgagccattt gacgcgtcga cgtcaagtac gtcactacgc nnnnnngctc catcgctgtg agggacgnca tgagccgcgc gcatcggtca agcacctcac ggcgcaggaa cgcctccccg acgaggacgc ggttgagaaa gaggtcagcg tcgaccccaa gcgtcacgcc gctaatgcca gcaccccttc ccttgtcagt tccccgcgtg ccggcgttga gaacggcagc gtgggtatgc tatcccgcag cacgcccttc tcgacaaggt gcacaaggcc cgcgaggaga cgctcgcggc gccgccctac cttgcggtct gtgagggcat ccacctcctt gaggtccaga gcgatgtgcc gctctaggtg ctgcatgatg ctctcgcttc tctctggcat cacaaagaaa cggcacacca ccgagcgcgc cacgtgcgca tgctcgcggg agtgctccgt ccaggaggtg tagggggtgt cgcacagcag acaaagctgc gactcacacg atgccgcgca tgtgctctcc ggcactgtcg tcatgcatcg cgcaggagta aggcggcagc atgaagaggt gaagacggtg gcgcgccggt ttccggcgaa actcaagcgt ggtgcaatca catccgcgct gtaactcggt cctttggcag cggtcgccgg gtggcgccag atcggcgccg tcgacaccag ggcggcgacg acgcggtaca gccgcagcag tgccgcatgt cctctcccga gctgagcctg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMQ AQLGRGHAAL LRLYRVVAAL VSTAPIWRHP ATAAKGPSYS ADVIAPRLSF AGNRRATVFT SSCCRLTPAR CMTTVPESTC AASCESQLCL LCDTPYTSWT EHSREHAHVA RSVVCRFFVM PERSESIMQH LERHIALDLK EVDALTDRKV GRRRERLLAG LVHLVEKGVL RDSIPTLPFS TPARGGTDKG RGAGISGVTL GVDADLFLNR VLVGEAFLRR EVLDRCARLM PSLTAMELNS VVTYLTSTRQ MARIFDELSL IELLVTRSGL ESAPANVNTA TAAATAAGRA AESASVEPLS ETAAAPLPDS DASTESPPAH QPCSSTLAAS LPSPPRWSLS REDKVYIMCA CLGELHRFHE QERPRSVISK AAADAIVLNV LAGHGRENLV SELVHETLQR IVDEGTVVWR QHQEDVKRRG RSSEANVAGA ARISARRAPR TDSTVSEDLC YVRTAPLPSA PSPRPASQVT LDSAAAEQLV SSYLPFNDVD QLSGAETDAV KATAEAPSRS SPDRTCTTSP ECISVLHTSE KDWGPLTAEL RKRAEDWNRL PFVPSTPELK ASKMPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagg ctcagctcgg gagaggacat gcggcactgc tgcggctgta ccgcgtcgtc gccgccctgg tgtcgacggc gccgatctgg cgccacccgg cgaccgctgc caaaggaccg agttacagcg cggatgtgat tgcaccacgc ttgagtttcg ccggaaaccg gcgcgccacc gtcttcacct cttcatgctg ccgccttact cctgcgcgat gcatgacgac agtgccggag agcacatgcg cggcatcgtg tgagtcgcag ctttgtctgc tgtgcgacac cccctacacc tcctggacgg agcactcccg cgagcatgcg cacgtggcgc gctcggtggt gtgccgtttc tttgtgatgc cagagagaag cgagagcatc atgcagcacc tagagcggca catcgctctg gacctcaagg aggtggatgc cctcacagac cgcaaggtag ggcggcgccg cgagcgtctc ctcgcgggcc ttgtgcacct tgtcgagaag ggcgtgctgc gggatagcat acccacgctg ccgttctcaa cgccggcacg cgggggaact gacaagggaa ggggtgctgg cattagcggc gtgacgcttg gggtcgacgc tgacctcttt ctcaaccgcg tcctcgtcgg ggaggcgttc ctgcgccgtg aggtgcttga ccgatgcgcg cggctcatgc cgtccctcac agcgatggag ctgaacagcg tagtgacgta cttgacgtcg acgcgtcaaa tggctcgcat ctttgacgag ctgtcgctga tagagcttct cgtgacgcgt tcggggttgg aaagcgctcc cgcgaacgtg aacaccgcca ccgctgctgc cactgccgcc ggaagagcgg cagagtcagc gagcgtagag ccgctgagcg agacagctgc ggcacctttg ccagacagcg acgcgagcac agagtcgcca cctgcgcatc agccctgctc ttccacactg gccgccagtc ttccctctcc cccacgctgg agcctgtccc gcgaggacaa ggtgtacatc atgtgcgcct gtctcggcga gctgcaccgc tttcacgagc aggagcgacc gcgctctgtc atctccaagg cagcagcaga tgctattgtg ctgaacgtgc ttgccggcca cgggcgcgag aaccttgtgt cggagttggt gcatgaaaca ttgcagcgta tcgtggacga ggggacggtg gtctggcggc agcatcagga ggatgtgaag cggcgcggtc gttcatctga agcaaacgtg gcaggggcag ctcgaatcag cgcgcggcgg gcacctcgca cggacagtac cgtatccgag gacttgtgct acgtgcgcac tgcaccgctg ccgtcagcgc cgtcaccgcg gccggcgtct caggttacac tggatagcgc agcagcggag cagctcgtgt cttcttactt gcccttcaat gatgtcgatc aacttagcgg cgccgagact gacgctgtga aagcgacagc agaagcgccg tcgaggtcga gtccggaccg cacctgcact acttctcccg agtgcatttc agtgctgcac acttcggaga aggactgggg gccgctcacg gcggagctgc ggaagcgtgc cgaggattgg aatcggctac cctttgtgcc tagcacaccc gagctaaaag cgtcgaagat gccgcgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at