SSGCID: Target Page

LemaA.19507.a

KREPB5

CENTER ID:	LemaA.19507.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.19507.a.B1.GE40868	full length		1	429

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LmjF.27.0340
RefSeq:	XP_003721840.1
UniProt:	E9AD11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRTCRSLRR FSAGPLDLAH SICRSAVVSS STRAASSQNS VAPRTGSSSF ARQAARMAPP QTTLSLDANL RNTLRSLLIE RLCSPTQPLP AKQLYDELTN RSRLVEEFHF SKTSRRITRQ RLALQFLRRY NVLLHGLFFY SEGTRTWNTT TEFHRLAILG ESVLLTEVRT RLLKLFPVMP YAAYVQSLPH MVGEEALAAA FDRYEMQNIV GAKPSNQRSG TPLTRMQKSH MLCAIVAEMY WFTARTKPTD LTHNNALFPP SDVLILHVLS THLLENLPAE LIYNVVEPIV ADIKRVWVNE PMSLPSQLRL TPRTVGALSL NAVPVDLRYT AKEDAVMSLQ KKHEEAHRRQ HALTREPDHS CVKSFMRPSC NYRIFDGPRY QILSSDNRVA PLPLAQERSS LPASAVCTVV DDADSAARAM ELAKMAEQW

NT Sequence

atgaagcgga catgtcgctc tctgcgaagg ttttccgcag gtccgcttga cttagcgcat tcgatctgta gaagcgcggt ggtttcttcc tctactcgcg ctgcctcatc acaaaattcg gtcgcgccgc gcacaggaag ctcttccttc gcgcgtcagg ccgcgcgaat ggcaccgccg cagacaactc tttcactgga cgccaacctg cgcaacaccc tcagaagtct gctcattgag cgtctgtgca gtccgacaca gcccctgccg gcaaagcagt tgtatgatga gctgacgaac cgctcgcgtc tcgtggaaga gtttcacttc tcgaagacgt cacgccgcat cacccgccag cggctggcac tgcagttctt gcgtcgatac aacgtgctgt tgcacggcct cttcttttat tcggagggca cgcgcacgtg gaatacgacg acggagtttc atcgtctcgc catccttgga gagtctgtgc tactaaccga agttcgcact cggctgctga agctcttccc tgtcatgccg tatgcggcgt acgtgcagag tttgcctcac atggtcggcg aggaagcgct ggcagcagct tttgaccggt atgagatgca gaacattgtc ggtgccaagc catctaacca gcgcagcggc acgccgctca ctcgaatgca gaaaagccat atgctgtgcg ctattgtagc ggagatgtac tggtttacag ctcgcacgaa gcccaccgac ttgacgcaca acaacgcgct cttccctcca tctgacgtgc tcatcttgca cgtgctgtcc acccacctcc tcgaaaattt gcctgcagag ctcatttaca acgtcgtaga gccgatcgtg gcggacatca agcgcgtatg ggtgaacgag ccgatgtcgt tgccgtcaca actgcgccta accccgcgga ctgtcggtgc cctctccctc aatgctgtcc ccgtcgatct ccgctacacc gccaaggaag acgctgtcat gtcgctccaa aaaaagcatg aggaggctca tcgccgacaa cacgcgctta cccgcgagcc ggaccacagc tgcgtcaagt ccttcatgcg accgtcgtgc aactaccgca tatttgacgg tccacggtat cagatactga gctccgataa tcgcgtcgca ccactaccgc ttgcacaaga gcggagcagt cttcccgctt cggctgtgtg caccgtcgtt gacgatgcgg acagcgccgc acgtgcgatg gagcttgcca agatggcgga gcagtggtga

Details for LemaA.19507.a.B1.GE40868

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK RTCRSLRRFS AGPLDLAHSI CRSAVVSSST RAASSQNSVA PRTGSSSFAR QAARMAPPQT TLSLDANLRN TLRSLLIERL CSPTQPLPAK QLYDELTNRS RLVEEFHFSK TSRRITRQRL ALQFLRRYNV LLHGLFFYSE GTRTWNTTTE FHRLAILGES VLLTEVRTRL LKLFPVMPYA AYVQSLPHMV GEEALAAAFD RYEMQNIVGA KPSNQRSGTP LTRMQKSHML CAIVAEMYWF TARTKPTDLT HNNALFPPSD VLILHVLSTH LLENLPAELI YNVVEPIVAD IKRVWVNEPM SLPSQLRLTP RTVGALSLNA VPVDLRYTAK EDAVMSLQKK HEEAHRRQHA LTREPDHSCV KSFMRPSCNY RIFDGPRYQI LSSDNRVAPL PLAQERSSLP ASAVCTVVDD ADSAARAMEL

Validated NT Sequence

caagctccat cgcacgtgcg gcgctgtccg catcgtcaac gacggtgcac acagccgaag cgggaagact gctccgctct tgtgcaagcg gtagtggtgc gacgcgatta tcggagctca gtatctgata ccgtggaccg tcaaatatgc ggtagttgca cgacggtcgc atgaaggact tgacgcagct gtggtccggc tcgcgggtaa gcgcgtgttg tcggcgatga gcctcctcat gctttttttg gagcgacatg acagcgtctt ccttggcggt gtagcggaga tcgacgggga cagcattgag ggagagggca ccgacagtcc gcggggttag gcgcagttgt gacggcaacg acatcggctc gttcacccat acgcgcttga tgtccgccac gatcggctct acgacgttgt aaatgagctc tgcaggcaaa ttttcgagga ggtgggtgga cagcacgtgc aagatgagca cgtcagatgg agggaagagc gcgttgttgt gcgtcaagtc ggtgggcttc gtgcgagctg taaaccagta catctccgct acaatagcgc acagcatatg gcttttctgc attcgagtga gcggcgtgcc gctgcgctgg ttagatggct tggcaccgac aatgttctgc atctcatacc ggtcaaaagc tgctgccagc gcttcctcgc cgaccatgtg aggcaaactc tgcacgtacg ccgcatacgg catgacaggg aagagcttca gcagccgagt gcgaacttcg gttagtagca cagactctcc aaggatggcg agacgatgaa actccgtcgt cgtattccac gtgcgcgtgc cctccgaata aaagaagagg ccgtgcaaca gcacgttgta tcgacgcaag aactgcagtg ccagccgctg gcgggtgatg cggcgtgacg tcttcgagaa gtgaaactct tccacgagac gcgagcggtt cgtcagctca tcatacaact gctttgccgg caggggctgt gtcggactgc acagacgctc aatgagcaga cttctgaggg tgttgcgcag gttggcgtcc agtgaaagag ttgtctgcgg cggtgccatt cgcgcggcct gacgcgcgaa ggaagagctt cctgtgcgcg gcgcgaccga attttgtgat gaggcagcgc gagtagagga agaaaccacc gcgcttctac agatcgaatg cgctaagtca agcggacctg cggaaaacct tcgcagagag cgacatgtcc gcttcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc ggacatgtcg ctctctgcga aggttttccg caggtccgct tgacttagcg cattcgatct gtagaagcgc ggtggtttct tcctctactc gcgctgcctc atcacaaaat tcggtcgcgc cgcgcacagg aagctcttcc ttcgcgcgtc aggccgcgcg aatggcaccg ccgcagacaa ctctttcact ggacgccaac ctgcgcaaca ccctcagaag tctgctcatt gagcgtctgt gcagtccgac acagcccctg ccggcaaagc agttgtatga tgagctgacg aaccgctcgc gtctcgtgga agagtttcac ttctcgaaga cgtcacgccg catcacccgc cagcggctgg cactgcagtt cttgcgtcga tacaacgtgc tgttgcacgg cctcttcttt tattcggagg gcacgcgcac gtggaatacg acgacggagt ttcatcgtct cgccatcctt ggagagtctg tgctactaac cgaagttcgc actcggctgc tgaagctctt ccctgtcatg ccgtatgcgg cgtacgtgca gagtttgcct cacatggtcg gcgaggaagc gctggcagca gcttttgacc ggtatgagat gcagaacatt gtcggtgcca agccatctaa ccagcgcagc ggcacgccgc tcactcgaat gcagaaaagc catatgctgt gcgctattgt agcggagatg tactggttta cagctcgcac gaagcccacc gacttgacgc acaacaacgc gctcttccct ccatctgacg tgctcatctt gcacgtgctg tccacccacc tcctcgaaaa tttgcctgca gagctcattt acaacgtcgt agagccgatc gtggcggaca tcaagcgcgt atgggtgaac gagccgatgt cgttgccgtc acaactgcgc ctaaccccgc ggactgtcgg tgccctctcc ctcaatgctg tccccgtcga tctccgctac accgccaagg aagacgctgt catgtcgctc caaaaaaagc atgaggaggc tcatcgccga caacacgcgc ttacccgcga gccggaccac agctgcgtca agtccttcat gcgaccgtcg tgcaactacc gcatatttga cggtccacgg tatcagatac tgagctccga taatcgcgtc gcaccactac cgcttgcaca agagcggagc agtcttcccg cttcggctgt gtgcaccgtc gttgacgatg cggacagcgc cgcacgtgcg atggagcttg ccaagatggc ggagcagtgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat