LemaA.19506.a

KREPB6

CENTER ID: LemaA.19506.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19506.a.B2.GE40968 ordered domain 1 99 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LmjF.29.1430
RefSeq: XP_003722223.1
UniProt: E9AE02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANLVRFAKI SAGTPRMSWV AAEVAEACVR QHGMMCRAAL RQPPRHFLHG QTRRAATVTA LSGVCRCLST AAPRSEPIST PVSPSSSPSP KQDPPATPRQ SVQTASLTLT AESVWETVPH WCELCCEPVN SWHNHLGKRD HICLEMILNS VVGSKGLWES DKVWDSIELF RSHYTKRPHG QPRTPSPIVK RLLKEGLYVP GPDHPLGMFY DAFDRGDPYA RREELHLLLL YLKECGMLCL APDVFPPAIF QGHLLLHSEV VPVLTRIFPD GEVRGISALS QMITGAYGLA AAYETCGMER LVPVSLLQHR GLNPELISVL SSVEEFDGVA ASDDATGGTG GEETLGEGAE GGTSDAPLST EQSMAMRSLS AAMPRAILGS LRWALAPEDT SPPPIIFQDH KRNAYLTTLA ARAARLLMSE LIFGRISEYV YRLEYVLRTD FGREYLHHRY EGHRTADKAS GRAASQRWLR KQCITPSLSN EGTVAYTGGG LAAHGLNGTV AAECAVRMHD EARKARMHNR RGLPKAPPSS PLARSVRFR
NT Sequence
atggcgaatc ttgttcgatt cgccaagata agcgcgggca ccccacggat gtcctgggtt gccgccgaag tggcagaggc ctgcgtacgg cagcacggta tgatgtgccg cgctgcgcta cggcagccac cgcggcactt cctacatggg cagacgcggc gggcggcgac cgtgaccgcg ctgtcaggtg tgtgccgctg tctgtctacg gcagcgccga ggtctgagcc catctcgact cctgtatcac cgagctcgtc cccgtcgccc aagcaggacc cgcctgccac ccctcggcag tccgtgcaaa ctgcaagcct cacactaaca gcggaatcgg tgtgggaaac ggtcccacac tggtgcgagc tgtgctgtga gcccgttaac agctggcaca accacctggg caagcgcgac catatttgtc tcgagatgat cctaaactcc gtagtcggca gcaaaggact ctgggaatcc gacaaagtgt gggacagcat tgagctgttt cgaagtcact acacgaagcg cccacacggg cagccacgaa cgccctcgcc gattgtgaaa cgcctcttga aggaggggct ctacgtgccg ggtcccgatc acccgcttgg catgttctac gacgcctttg accgtggtga cccgtacgcg cggcgggagg agctgcatct gctgctcttg tacttgaagg agtgtgggat gctgtgcctc gcccccgacg tgttccctcc agcgatcttc caaggacact tgctcttgca cagcgaggtg gtgccggtgc tgacgcgcat cttcccagac ggggaggtgc gcggaatctc ggcactgtcg cagatgataa caggcgccta tgggctggca gcggcgtatg aaacgtgtgg gatggagcgg ctggtgccgg tctccttgct gcaacaccga gggttgaacc cagagctcat ttcagtttta tcgtcggtag aggagttcga cggcgttgcg gcaagcgatg acgcgacggg cggcaccggc ggcgaagaga ctctcggcga aggcgccgaa ggtgggacta gcgatgcacc cttatcaacc gaacaatcca tggcaatgcg ctccttgagc gccgccatgc cccgtgccat cctcggctcc ctccgctggg ccctcgctcc agaggacacg agccccccgc cgatcatctt tcaggaccac aagcgcaacg cctacctgac caccttggca gcgcgcgcgg cacgcctgct gatgtccgag ctgatctttg ggcgcatctc cgagtacgta tatcgtttag agtacgttct gcgcacagac ttcgggcgcg agtatctgca ccatcgatac gaaggccatc gaacggcgga caaggccagc ggccgcgcgg cctcgcagcg ctggctgcgc aagcagtgca ttaccccttc attgtcgaac gagggaactg ttgcctatac aggtggtggg ctcgccgccc atgggctcaa cggcaccgtg gcggctgagt gcgccgttcg gatgcacgac gaggctcgga aggcgcgcat gcacaatcgc cgcggactgc cgaaggcgcc acccagctcc cctttggccc gcagtgtacg ctttcggtga
Details for LemaA.19506.a.B2.GE40968
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 28kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR QSVQTASLTL TAESVWETVP HWCELCCEPV NSWHNHLGKR DHICLEMILN SVVGSKGLWE SDKVWDSIEL FRSHYTKRPH GQPRTPSPIV KRLLKEGLYV PGPDHPLGMF YDAFDRGDPY ARREELHLLL LYLKECGMLC LAPDVFPPAI FQGHLLLHSE VVPVLTRIFP DGEVRGISAL SQMITGAYGL AAAYETCGME RLVPVSLLQH RGLNPELISV LSSVEEFDGV AASDDAT
Validated NT Sequence
atcttactca cttacgtcgc gtcatcgctt gccgcaacgc cgtcgaactc ctctaccgac gataaaactg aaatgagctc tgggttcaac cctcggtgtt gcagcaagga gaccggcacc agccgctcca tcccacacgt ttcatacgcc gctgccagcc cataggcgcc tgttatcatc tgcgacagtg ccgagattcc gcgcacctcc ccgtctggga agatgcgcgt cagcaccggc accacctcgc tgtgcaagag caagtgtcct tggaagatcg ctggagggaa cacgtcgggg gcgaggcaca gcatcccaca ctccttcaag tacaagagca gcagatgcag ctcctcccgc cgcgcgtacg ggtcaccacg gtcaaaggcg tcgtagaaca tgccaagcgg gtgatcggga cccggcacgt agagcccctc cttcaagagg cgtttcacaa tcggcgaggg cgttcgtggc tgcccgtgtg ggcgcttcgt gtagtgactt cgaaacagct caatgctgtc ccacactttg tcggattccc agagtccttt gctgccgact acggagttta ggatcatctc gagacaaata tggtcgcgct tgcccaggtg gttgtgccag ctgttaacgg gctcacagca cagctcgcac cagtgtggga ccgtttccca caccgattcc gctgttagtg tgaggcttgc agtttgcacg gactgccgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHRQ SVQTASLTLT AESVWETVPH WCELCCEPVN SWHNHLGKRD HICLEMILNS VVGSKGLWES DKVWDSIELF RSHYTKRPHG QPRTPSPIVK RLLKEGLYVP GPDHPLGMFY DAFDRGDPYA RREELHLLLL YLKECGMLCL APDVFPPAIF QGHLLLHSEV VPVLTRIFPD GEVRGISALS QMITGAYGLA AAYETCGMER LVPVSLLQHR GLNPELISVL SSVEEFDGVA ASDDAT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcggcagt ccgtgcaaac tgcaagcctc acactaacag cggaatcggt gtgggaaacg gtcccacact ggtgcgagct gtgctgtgag cccgttaaca gctggcacaa ccacctgggc aagcgcgacc atatttgtct cgagatgatc ctaaactccg tagtcggcag caaaggactc tgggaatccg acaaagtgtg ggacagcatt gagctgtttc gaagtcacta cacgaagcgc ccacacgggc agccacgaac gccctcgccg attgtgaaac gcctcttgaa ggaggggctc tacgtgccgg gtcccgatca cccgcttggc atgttctacg acgcctttga ccgtggtgac ccgtacgcgc ggcgggagga gctgcatctg ctgctcttgt acttgaagga gtgtgggatg ctgtgcctcg cccccgacgt gttccctcca gcgatcttcc aaggacactt gctcttgcac agcgaggtgg tgccggtgct gacgcgcatc ttcccagacg gggaggtgcg cggaatctcg gcactgtcgc agatgataac aggcgcctat gggctggcag cggcgtatga aacgtgtggg atggagcggc tggtgccggt ctccttgctg caacaccgag ggttgaaccc agagctcatt tcagttttat cgtcggtaga ggagttcgac ggcgttgcgg caagcgatga cgcgacgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at