LemaA.19503.a

KREPB7

CENTER ID: LemaA.19503.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19503.a.B1.GE40865 full length 1 533
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LmjF.15.1270
RefSeq: XP_001682043.1
UniProt: Q4QF55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSSSSLLRL SPIRRAAVAV MTRALRATST NMSSRTRVTM ANAAGDNGAN ILRPPYPTEG GSVPVYSMMH GQIDKRNWVF RNPQWCELCN EPVSRWLDHQ GRKDHALLDL HYSQLAEWPR RWNPEAVLST FMQHLGIDSV EPYHALFSKL DSEGRTELYA MLVKLEEAGM VHFGSSRDTY LSRMVGGMRG FDHQGALVLH ECLMGPFLRL FPNGLIQDYS NLLDFISCGY NMETVYDMCG MYTLDKVALR ERYGPSSPAA MGLGGVATCS SASRGYENDL LASLSAASSS SLSSAASDSA ANAASAPGGG SGTKGRLAQL QAARAAKLQE KSEEEAFSRK TAFVRQILGQ LRWLLMPEQE HPAGFTFPPH IITLGELCLK GLVVAIIEAR LCEYMVRAEP VWVSFGLERR KLKQSAIANA GDVVPQPVQY SYRPMARDLR DLYSTQPSAL DAMVQAGLLK KKLPTLKAFG KHEASSMHAK LVKGGAAAVD SSRESPSSAS APPCCEQATA TDHYWQATPA KGSARSREGA SRR
NT Sequence
atgccctcgt cgtcgtcact gctgaggttg tcgcccattc gccgcgccgc tgtcgccgtg atgacgcggg cgttgcgggc cacttccact aacatgtcct ccaggacacg cgtcacgatg gccaacgccg cgggtgacaa cggcgcgaac atcctgcggc ctccataccc gactgagggc ggcagtgttc ccgtatactc gatgatgcac gggcagattg acaagcgcaa ctgggtgttt cgcaacccgc agtggtgcga gctgtgcaac gagccggttt cccgttggtt agatcatcag ggtcgcaagg accacgcatt gttggatttg cactactcgc agctggccga gtggccgcgt cgatggaacc ccgaggcggt gctgtcgacg ttcatgcaac acctcggaat cgacagcgtg gagccgtacc acgctctctt ctccaagctg gacagcgagg gccgcacaga gctgtacgcg atgctcgtca aactggaaga ggctggtatg gtgcacttcg gcagcagccg cgacacctat ctcagccgca tggtcggtgg gatgcgcggg ttcgaccatc aaggcgcgct ggtgttgcac gagtgcctga tgggcccctt tctgcgactc tttccaaacg gcctcataca agattactcg aacctcctcg acttcatttc gtgcggctac aatatggaga cggtgtacga catgtgcggt atgtacacgc tggacaaggt ggcactgcgg gagcgttacg gaccgagctc gcccgccgcg atggggctgg gcggcgtcgc gacgtgctcc tccgcgtcgc gcggctacga aaacgacttg cttgcgtcct tgtcggcggc ctcgtcgtcg tcgttgtcgt ccgccgcctc cgacagcgcg gccaacgctg cgagcgcccc aggtggcggc agcggtacca agggccggct cgcgcagctg caagccgcac gggccgcgaa gttgcaggag aagtcagagg aggaagcctt ctcgcgcaag acggcattcg tgcgtcagat cctcgggcag ctgcggtggc tgctgatgcc agagcaggag cacccggccg gtttcacgtt cccaccgcac atcatcaccc tgggcgagct gtgcctgaag gggctggtgg tcgccattat cgaggcacgc ctctgcgagt acatggtacg cgccgagcct gtctgggttt ccttcggcct cgagcggcgg aagctcaagc agagcgcgat cgcgaatgcc ggagacgtgg tgccgcagcc ggtgcagtac tcgtaccgcc ccatggcccg cgacctgcgc gacctgtact ccacccaacc aagcgcgctg gatgcaatgg tgcaggcggg cctactgaag aagaagctac cgacgctgaa ggcgtttggg aagcacgagg cgtcatcgat gcatgcgaaa ctagtaaaag gaggggcggc agcggtggac agctcacgtg aaagcccctc atcagcatct gcacccccct gctgcgagca ggccactgca acagatcatt attggcaagc caccccagcg aaaggaagcg caagatcaag agagggtgcg tcgaggcggt ga
Details for LemaA.19503.a.B1.GE40865
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP SSSSLLRLSP IRRAAVAVMT RALRATSTNM SSRTRVTMAN AAGDNGANIL RPPYPTEGGS VPVYSMMHGQ IDKRNWVFRN PQWCELCNEP VSRWLDHQGR KDHALLDLHY SQLAEWPRRW NPEAVLSTFM QHLGIDSVEP YHALFSKLDS EGRTELYAML VKLEEAGMVH FGSSRDTYLS RMVGGMRGFD HQGALVLHEC LMGPFLRLFP NGLIQDYSNL LDFISCGYNM ETVYDMCGMY TLDKVALRER YGPSSPAAMG LGGVATCSSA SRGYENDLLA SLSAASSSSL SSAASDSAAN AASAPGGGSG TKGRLAQLQA ARAAKLQEKS EEEAFSRKTA FVRQILGQLR WLLMPEQEHP AGFTFPPHII TLGELCLKGL VVAIIEARLC EYMVRAEPVW VSFGLERRKL KQSAIANAGD VVPQPVQYSY RPMARDLRDL YSTQPSALDA MVQAGLLKKK LPTLKAFGKH EASSMHAKLV KGGAAAVDSS RESPSSASAP PCCEQATATD HYWQATPAKG SARSREGASR R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccct cgtcgtcgtc actgctgagg ttgtcgccca ttcgccgcgc cgctgtcgcc gtgatgacgc gggcgttgcg ggccacttcc actaacatgt cctccaggac acgcgtcacg atggccaacg ccgcgggtga caacggcgcg aacatcctgc ggcctccata cccgactgag ggcggcagtg ttcccgtata ctcgatgatg cacgggcaga ttgacaagcg caactgggtg tttcgcaacc cgcagtggtg cgagctgtgc aacgagccgg tttcccgttg gttagatcat cagggtcgca aggaccacgc attgttggat ttgcactact cgcagctggc cgagtggccg cgtcgatgga accccgaggc ggtgctgtcg acgttcatgc aacacctcgg aatcgacagc gtggagccgt accacgctct cttctccaag ctggacagcg agggccgcac agagctgtac gcgatgctcg tcaaactgga agaggctggt atggtgcact tcggcagcag ccgcgacacc tatctcagcc gcatggtcgg tgggatgcgc gggttcgacc atcaaggcgc gctggtgttg cacgagtgcc tgatgggccc ctttctgcga ctctttccaa acggcctcat acaagattac tcgaacctcc tcgacttcat ttcgtgcggc tacaatatgg agacggtgta cgacatgtgc ggtatgtaca cgctggacaa ggtggcactg cgggagcgtt acggaccgag ctcgcccgcc gcgatggggc tgggcggcgt cgcgacgtgc tcctccgcgt cgcgcggcta cgaaaacgac ttgcttgcgt ccttgtcggc ggcctcgtcg tcgtcgttgt cgtccgccgc ctccgacagc gcggccaacg ctgcgagcgc cccaggtggc ggcagcggta ccaagggccg gctcgcgcag ctgcaagccg cacgggccgc gaagttgcag gagaagtcag aggaggaagc cttctcgcgc aagacggcat tcgtgcgtca gatcctcggg cagctgcggt ggctgctgat gccagagcag gagcacccgg ccggtttcac gttcccaccg cacatcatca ccctgggcga gctgtgcctg aaggggctgg tggtcgccat tatcgaggca cgcctctgcg agtacatggt acgcgccgag cctgtctggg tttccttcgg cctcgagcgg cggaagctca agcagagcgc gatcgcgaat gccggagacg tggtgccgca gccggtgcag tactcgtacc gccccatggc ccgcgacctg cgcgacctgt actccaccca accaagcgcg ctggatgcaa tggtgcaggc gggcctactg aagaagaagc taccgacgct gaaggcgttt gggaagcacg aggcgtcatc gatgcatgcg aaactagtaa aaggaggggc ggcagcggtg gacagctcac gtgaaagccc ctcatcagca tctgcacccc cctgctgcga gcaggccact gcaacagatc attattggca agccacccca gcgaaaggaa gcgcaagatc aagagagggt gcgtcgaggc ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat