CENTER ID: | LemaA.19501.a |
ORGANISM: | Leishmania major Friedlin |
ASSOCIATED DISEASE: | Cutaneous leishmaniasis |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
unclassified |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LemaA.19501.a.B1.GE40864 | full length | 1 | 663 | ||
LemaA.19501.a.B2.GE40697 | C-term domain | 380 | 657 | ||
LemaA.19501.a.B3.GE40698 | J-binding domain | 116 | 284 | ||
LemaA.19501.a.B4.GE41053 | N-extended J-binding domain | 83 | 284 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LemaA.19501.a.B4.PW38195 | N-extended J-binding domain | 83 | 284 |
METHOD | RESULTS |
---|---|
comparative modelling | Robetta_79387 |
RESOURCE | REFERENCE ID |
---|---|
EuPathDB: | TriTrypDB:LmjF.36.0390 |
UniProt: | Q4Q239 |
HARVESTED ON: | data unavailable |
SEQUENCED ON: | 12/6/2016 |
EXPECTED MW: | 74kDa |
OBSERVED MW: | 0kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 94 |
PERCENT COVERAGE: | 45 |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 33kDa |
OBSERVED MW: | 33kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 21kDa |
OBSERVED MW: | 21kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 1/4/2017 |
SEQUENCED ON: | 1/5/2017 |
EXPECTED MW: | 25kDa |
OBSERVED MW: | 25kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 2/28/2017 |
CONCENTRATION: | 25mg/ml |
OBSERVED MW: | 25kDa |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 5 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |