LemaA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID: LemaA.19501.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19501.a.B1.GE40864 full length 1 663
LemaA.19501.a.B2.GE40697 C-term domain 380 657
LemaA.19501.a.B3.GE40698 J-binding domain 116 284
LemaA.19501.a.B4.GE41053 N-extended J-binding domain 83 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.19501.a.B4.PW38195 N-extended J-binding domain 83 284

Molecular models

METHOD RESULTS
comparative modelling Robetta_79387

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.36.0390
UniProt: Q4Q239

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSKRTEGTA ADEERRRTSA ASSGSSSVSS TDSDEASSSV SDSSSSGSGS SSDSESSDSE RPSSTPQRQS AAAAERATST QQLQQQRHVQ LNPELRFERP DKEYIPLPPL RDMQDMVKVL FLLSTDKKRY PDGRHRTLDY FRVAVEMFVT EVRQEYKRQY QQAQRNGRAM QRFTWKNSGE LAICFACCCD NVKLLYDSLQ PGPLKPLWDA FVGQLPPMLI VQSRVPETML SSQTYHTKYM DWVKGGTRYG GEQSTANVRF PSVADRRVKV ETYLRSGAGY KIEAVQVGST PAAPAAVPAA VAPRPSLAAA AAPMQALETR APFAGRLPGT GSAKVPVAPG VGGPRPPVKV PMKHVGGGVA HGSVTTAAAP PLASTSVKQM PPEMKHLFWL SVLAHVKGGE ASRAFPDDRL EALPGLLRLC EMTEHTEEMC ILMDVLAASS SGVQTVFERL GGLLSFRRFA SHFIQSEEAE PLMQLVEQLA RWRVAAWGST SQQAWQTDMN QRRTRDLMWL RTLPLIPEAK RAAWGALLLV MEQRYIFKAP RPDDTTAVDN PLKRRRSEVE LGDLHAVLSD NVDMQRWVVP RVNVFSAVRL PVELRVPPLC TKLEENTVRF LNAKREYAEK MQVTWRERLE KILSAQPRAP GPAVPVPLWY DPYALLRVAS PQP
NT Sequence
atgtcctcaa aacgcaccga gggcacagct gctgacgagg agcgccggcg gacgtcggcc gcgtctagcg gctcatcgag cgtcagcagt accgactctg acgaagccag cagcagtgtc tccgacagca gcagcagcgg cagcggcagc tcctccgact ccgaatcgtc cgatagcgag cgcccgtcgt ccaccccgca gcgccaaagt gccgctgcag cagagagagc cacgtctacg cagcagctac aacagcagcg gcatgtgcag ctgaatcccg agctgcgctt cgagcggcca gacaaggagt acatcccgct tccacctctc agagacatgc aggacatggt caaggttctg tttctcttga gcaccgataa gaaacgctat ccggatgggc gtcaccgcac gctggactac ttccgtgtcg cggtggagat gtttgtgacg gaagtgcgac aggagtacaa acggcagtac cagcaggcgc agcgcaacgg ccgtgcgatg cagcggttca cgtggaaaaa ctcgggcgaa ctggcgattt gctttgcctg ctgctgcgat aatgtgaagc ttctgtacga cagccttcaa cctggccccc tcaagccgct ctgggacgcc tttgtcggcc agctcccacc catgctcatc gtacagagcc gagtgccgga gacgatgctg tcatcccaaa cgtaccacac caagtacatg gactgggtga agggcggcac acgttacggt ggcgagcagt cgaccgcgaa tgtccgcttc ccctccgtgg cagaccgtcg cgtaaaggtt gaaacatacc tccggagtgg cgcggggtac aagatcgagg ctgtgcaggt ggggtcgacg cccgctgcgc cggcggctgt ccccgccgcg gtggcgcctc gcccttcttt ggccgctgcc gcggctccga tgcaggcttt agagactcgc gcgcctttcg cggggcgact gcccggcaca ggctcggcta aggtgccggt cgctcccggt gtcggtggac ctcgcccccc agtgaaggtt cccatgaagc acgtcggcgg cggtgttgcg cacggaagtg tgacgaccgc cgccgctccc ccgcttgcct ccaccagtgt gaagcagatg ccgccggaga tgaagcatct gttttggttg tcggtgctgg cgcacgtaaa gggtggggaa gcgtcccggg cgtttccgga tgaccgactg gaggcgctgc ctggcctgct gcggctgtgc gagatgacag aacacacgga ggagatgtgc atcttgatgg atgtgctggc ggcctcgagc agcggcgttc agacagtctt cgagcggctt ggcggccttc tgtcattccg ccggtttgcc tcccatttca tccagagtga ggaggcagag ccgctcatgc aactcgtcga gcagctcgcg cggtggcgcg tcgcggcatg gggcagtacc tcacagcagg catggcagac cgatatgaac caacggcgca cacgcgatct gatgtggctc cgtaccctgc ccctcatccc ggaggcgaag cgggcggcgt ggggagcgct gctgctggtg atggagcaac gctatatttt caaggccccg cgccctgatg acacgaccgc cgttgacaat ccactgaagc ggcggcgatc cgaggtcgag ctcggcgacc tgcacgctgt gctgagtgac aacgtcgaca tgcaaaggtg ggtggtgccc cgggtcaatg tgttctccgc tgtccgccta ccagtggagc tgcgcgtgcc gccactgtgc acaaagttgg aggagaatac tgtccgcttc ctcaacgcca agcgcgagta cgccgagaag atgcaggtga cgtggcgtga gaggctagag aagattctga gcgcgcaacc tcgtgctccg ggtcccgccg taccggtgcc tctctggtat gacccgtacg cgctgctgag ggtggcgtcc ccgcaaccat ga
Details for LemaA.19501.a.B1.GE40864
HARVESTED ON: data unavailable
SEQUENCED ON: 12/6/2016
EXPECTED MW: 74kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMS SKRTEGTAAD EERRRTSAAS SGSSSVSSTD SDEASSSVSD SSSSGSGSSS DSESSDSERP SSTPQRQSAA AAERATSTQQ LQQQRHVQLN PELRFERPDK EYIPLPPLRD MQDMVKVLFL LSTDKKRYPD GRHRTLDYFR VAVEMFVTEV RQEYKRQYQQ AQRNGRAMQR FTWKNSGELA ICFACCCDNV KLLYDSLQPG PLKPLWDAFV GQLPPMLIVQ SRVPETMLSS QTYHTKYMDW VKGGTRYGGE QSTANVRFPS VAXRRVKVET YLRXXAXXKX XAVXXXXXXA XXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc tcaaaacgca ccgagggcac agctgctgac gaggagcgcc ggcggacgtc ggccgcgtct agcggctcat cgagcgtcag cagtaccgac tctgacgaag ccagcagcag tgtctccgac agcagcagca gcggcagcgg cagctcctcc gactccgaat cgtccgatag cgagcgcccg tcgtccaccc cgcagcgcca aagtgccgct gcagcagaga gagccacgtc tacgcagcag ctacaacagc agcggcatgt gcagctgaat cccgagctgc gcttcgagcg gccagacaag gagtacatcc cgcttccacc tctcagagac atgcaggaca tggtcaaggt tctgtttctc ttgagcaccg ataagaaacg ctatccggat gggcgtcacc gcacgctgga ctacttccgt gtcgcggtgg agatgtttgt gacggaagtg cgacaggagt acaaacggca gtaccagcag gcgcagcgca acggccgtgc gatgcagcgg ttcacgtgga aaaactcggg cgaactggcg atttgctttg cctgctgctg cgataatgtg aagcttctgt acgacagcct tcaacctggc cccctcaagc cgctctggga cgcctttgtc ggccagctcc cacccatgct catcgtacag agccgagtgc cggagacgat gctgtcatcc caaacgtacc acaccaagta catggactgg gtgaagggcg gcacacgtta cggtggcgag cagtcgaccg cgaatgtccg cttcccctcc gtggcagncc gtcgcgtaaa ggttgaaaca tacctccgga ntnncgcngn gnacaagatn nnngctgtnn nnnnnnnnnn nannncngcn nnnnnnnnnn nnnnncc
Expected Protein Sequence
MAHHHHHHMS SKRTEGTAAD EERRRTSAAS SGSSSVSSTD SDEASSSVSD SSSSGSGSSS DSESSDSERP SSTPQRQSAA AAERATSTQQ LQQQRHVQLN PELRFERPDK EYIPLPPLRD MQDMVKVLFL LSTDKKRYPD GRHRTLDYFR VAVEMFVTEV RQEYKRQYQQ AQRNGRAMQR FTWKNSGELA ICFACCCDNV KLLYDSLQPG PLKPLWDAFV GQLPPMLIVQ SRVPETMLSS QTYHTKYMDW VKGGTRYGGE QSTANVRFPS VADRRVKVET YLRSGAGYKI EAVQVGSTPA APAAVPAAVA PRPSLAAAAA PMQALETRAP FAGRLPGTGS AKVPVAPGVG GPRPPVKVPM KHVGGGVAHG SVTTAAAPPL ASTSVKQMPP EMKHLFWLSV LAHVKGGEAS RAFPDDRLEA LPGLLRLCEM TEHTEEMCIL MDVLAASSSG VQTVFERLGG LLSFRRFASH FIQSEEAEPL MQLVEQLARW RVAAWGSTSQ QAWQTDMNQR RTRDLMWLRT LPLIPEAKRA AWGALLLVME QRYIFKAPRP DDTTAVDNPL KRRRSEVELG DLHAVLSDNV DMQRWVVPRV NVFSAVRLPV ELRVPPLCTK LEENTVRFLN AKREYAEKMQ VTWRERLEKI LSAQPRAPGP AVPVPLWYDP YALLRVASPQ P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct caaaacgcac cgagggcaca gctgctgacg aggagcgccg gcggacgtcg gccgcgtcta gcggctcatc gagcgtcagc agtaccgact ctgacgaagc cagcagcagt gtctccgaca gcagcagcag cggcagcggc agctcctccg actccgaatc gtccgatagc gagcgcccgt cgtccacccc gcagcgccaa agtgccgctg cagcagagag agccacgtct acgcagcagc tacaacagca gcggcatgtg cagctgaatc ccgagctgcg cttcgagcgg ccagacaagg agtacatccc gcttccacct ctcagagaca tgcaggacat ggtcaaggtt ctgtttctct tgagcaccga taagaaacgc tatccggatg ggcgtcaccg cacgctggac tacttccgtg tcgcggtgga gatgtttgtg acggaagtgc gacaggagta caaacggcag taccagcagg cgcagcgcaa cggccgtgcg atgcagcggt tcacgtggaa aaactcgggc gaactggcga tttgctttgc ctgctgctgc gataatgtga agcttctgta cgacagcctt caacctggcc ccctcaagcc gctctgggac gcctttgtcg gccagctccc acccatgctc atcgtacaga gccgagtgcc ggagacgatg ctgtcatccc aaacgtacca caccaagtac atggactggg tgaagggcgg cacacgttac ggtggcgagc agtcgaccgc gaatgtccgc ttcccctccg tggcagaccg tcgcgtaaag gttgaaacat acctccggag tggcgcgggg tacaagatcg aggctgtgca ggtggggtcg acgcccgctg cgccggcggc tgtccccgcc gcggtggcgc ctcgcccttc tttggccgct gccgcggctc cgatgcaggc tttagagact cgcgcgcctt tcgcggggcg actgcccggc acaggctcgg ctaaggtgcc ggtcgctccc ggtgtcggtg gacctcgccc cccagtgaag gttcccatga agcacgtcgg cggcggtgtt gcgcacggaa gtgtgacgac cgccgccgct cccccgcttg cctccaccag tgtgaagcag atgccgccgg agatgaagca tctgttttgg ttgtcggtgc tggcgcacgt aaagggtggg gaagcgtccc gggcgtttcc ggatgaccga ctggaggcgc tgcctggcct gctgcggctg tgcgagatga cagaacacac ggaggagatg tgcatcttga tggatgtgct ggcggcctcg agcagcggcg ttcagacagt cttcgagcgg cttggcggcc ttctgtcatt ccgccggttt gcctcccatt tcatccagag tgaggaggca gagccgctca tgcaactcgt cgagcagctc gcgcggtggc gcgtcgcggc atggggcagt acctcacagc aggcatggca gaccgatatg aaccaacggc gcacacgcga tctgatgtgg ctccgtaccc tgcccctcat cccggaggcg aagcgggcgg cgtggggagc gctgctgctg gtgatggagc aacgctatat tttcaaggcc ccgcgccctg atgacacgac cgccgttgac aatccactga agcggcggcg atccgaggtc gagctcggcg acctgcacgc tgtgctgagt gacaacgtcg acatgcaaag gtgggtggtg ccccgggtca atgtgttctc cgctgtccgc ctaccagtgg agctgcgcgt gccgccactg tgcacaaagt tggaggagaa tactgtccgc ttcctcaacg ccaagcgcga gtacgccgag aagatgcagg tgacgtggcg tgagaggcta gagaagattc tgagcgcgca acctcgtgct ccgggtcccg ccgtaccggt gcctctctgg tatgacccgt acgcgctgct gagggtggcg tccccgcaac catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LemaA.19501.a.B2.GE40697
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP PEMKHLFWLS VLAHVKGGEA SRAFPDDRLE ALPGLLRLCE MTEHTEEMCI LMDVLAASSS GVQTVFERLG GLLSFRRFAS HFIQSEEAEP LMQLVEQLAR WRVAAWGSTS QQAWQTDMNQ RRTRDLMWLR TLPLIPEAKR AAWGALLLVM EQRYIFKAPR PDDTTAVDNP LKRRRSEVEL GDLHAVLSDN VDMQRWVVPR VNVFSAVRLP VELRVPPLCT KLEENTVRFL NAKREYAEKM QVTWRERLEK ILSAQPRAPG PAVPVPLWYX XYALLR
Validated NT Sequence
atggctcacc accaccacca ccatatgccg ccggagatga agcatctgtt ttggttgtcg gtgctggcgc acgtaaaggg tggggaagcg tcccgggcgt ttccggatga ccgactggag gcgctgcctg gcctgctgcg gctgtgcgag atgacagaac acacggagga gatgtgcatc ttgatggatg tgctggcggc ctcgagcagc ggcgttcaga cagtcttcga gcggcttggc ggccttctgt cattccgccg gtttgcctcc catttcatcc agagtgagga ggcagagccg ctcatgcaac tcgtcgagca gctcgcgcgg tggcgcgtcg cggcatgggg cagtacctca cagcaggcat ggcagaccga tatgaaccaa cggcgcacac gcgatctgat gtggctccgt accctgcccc tcatcccgga ggcgaagcgg gcggcgtggg gagcgctgct gctggtgatg gagcaacgct atattttcaa ggccccgcgc cctgatgaca cgaccgccgt tgacaatcca ctgaagcggc ggcgatccga ggtcgagctc ggcgacctgc acgctgtgct gagtgacaac gtcgacatgc aaaggtgggt ggtgccccgg gtcaatgtgt tctccgctgt ccgcctacca gtggagctgc gcgtgccgcc actgtgcaca aagttggagg agaatactgt ccgcttcctc aacgccaagc gcgagtacgc cgagaagatg caggtgacgt ggcgtgagag gctagagaag attctgagcg cgcaacctcg tgctccgggt cccgccgtac cggtgcctct ctggtatgnn ncgtacgcgc tgctgaggta agtgagtaag at
Expected Protein Sequence
MAHHHHHHMP PEMKHLFWLS VLAHVKGGEA SRAFPDDRLE ALPGLLRLCE MTEHTEEMCI LMDVLAASSS GVQTVFERLG GLLSFRRFAS HFIQSEEAEP LMQLVEQLAR WRVAAWGSTS QQAWQTDMNQ RRTRDLMWLR TLPLIPEAKR AAWGALLLVM EQRYIFKAPR PDDTTAVDNP LKRRRSEVEL GDLHAVLSDN VDMQRWVVPR VNVFSAVRLP VELRVPPLCT KLEENTVRFL NAKREYAEKM QVTWRERLEK ILSAQPRAPG PAVPVPLWYD PYALLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc cggagatgaa gcatctgttt tggttgtcgg tgctggcgca cgtaaagggt ggggaagcgt cccgggcgtt tccggatgac cgactggagg cgctgcctgg cctgctgcgg ctgtgcgaga tgacagaaca cacggaggag atgtgcatct tgatggatgt gctggcggcc tcgagcagcg gcgttcagac agtcttcgag cggcttggcg gccttctgtc attccgccgg tttgcctccc atttcatcca gagtgaggag gcagagccgc tcatgcaact cgtcgagcag ctcgcgcggt ggcgcgtcgc ggcatggggc agtacctcac agcaggcatg gcagaccgat atgaaccaac ggcgcacacg cgatctgatg tggctccgta ccctgcccct catcccggag gcgaagcggg cggcgtgggg agcgctgctg ctggtgatgg agcaacgcta tattttcaag gccccgcgcc ctgatgacac gaccgccgtt gacaatccac tgaagcggcg gcgatccgag gtcgagctcg gcgacctgca cgctgtgctg agtgacaacg tcgacatgca aaggtgggtg gtgccccggg tcaatgtgtt ctccgctgtc cgcctaccag tggagctgcg cgtgccgcca ctgtgcacaa agttggagga gaatactgtc cgcttcctca acgccaagcg cgagtacgcc gagaagatgc aggtgacgtg gcgtgagagg ctagagaaga ttctgagcgc gcaacctcgt gctccgggtc ccgccgtacc ggtgcctctc tggtatgacc cgtacgcgct gctgaggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LemaA.19501.a.B3.GE40698
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV KVLFLLSTDK KRYPDGRHRT LDYFRVAVEM FVTEVRQEYK RQYQQAQRNG RAMQRFTWKN SGELAICFAC CCDNVKLLYD SLQPGPLKPL WDAFVGQLPP MLIVQSRVPE TMLSSQTYHT KYMDWVKGGT RYGGEQSTAN VRFPSVADRR VKVETYLRSG AGYKIEA
Validated NT Sequence
atggctcacc accaccacca ccatatggtc aaggttctgt ttctcttgag caccgataag aaacgctatc cggatgggcg tcaccgcacg ctggactact tccgtgtcgc ggtggagatg tttgtgacgg aagtgcgaca ggagtacaaa cggcagtacc agcaggcgca gcgcaacggc cgtgcgatgc agcggttcac gtggaaaaac tcgggcgaac tggcgatttg ctttgcctgc tgctgcgata atgtgaagct tctgtacgac agccttcaac ctggccccct caagccgctc tgggacgcct ttgtcggcca gctcccaccc atgctcatcg tacagagccg agtgccggag acgatgctgt catcccaaac gtaccacacc aagtacatgg actgggtgaa gggcggcaca cgttacggtg gcgagcagtc gaccgcgaat gtccgcttcc cctccgtggc agaccgtcgc gtaaaggttg aaacatacct ccggagtggc gcggggtaca agatcgaggc ttaagtgagt aagat
Expected Protein Sequence
MAHHHHHHMV KVLFLLSTDK KRYPDGRHRT LDYFRVAVEM FVTEVRQEYK RQYQQAQRNG RAMQRFTWKN SGELAICFAC CCDNVKLLYD SLQPGPLKPL WDAFVGQLPP MLIVQSRVPE TMLSSQTYHT KYMDWVKGGT RYGGEQSTAN VRFPSVADRR VKVETYLRSG AGYKIEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtca aggttctgtt tctcttgagc accgataaga aacgctatcc ggatgggcgt caccgcacgc tggactactt ccgtgtcgcg gtggagatgt ttgtgacgga agtgcgacag gagtacaaac ggcagtacca gcaggcgcag cgcaacggcc gtgcgatgca gcggttcacg tggaaaaact cgggcgaact ggcgatttgc tttgcctgct gctgcgataa tgtgaagctt ctgtacgaca gccttcaacc tggccccctc aagccgctct gggacgcctt tgtcggccag ctcccaccca tgctcatcgt acagagccga gtgccggaga cgatgctgtc atcccaaacg taccacacca agtacatgga ctgggtgaag ggcggcacac gttacggtgg cgagcagtcg accgcgaatg tccgcttccc ctccgtggca gaccgtcgcg taaaggttga aacatacctc cggagtggcg cggggtacaa gatcgaggct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LemaA.19501.a.B4.GE41053
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML QQQRHVQLNP ELRFERPDKE YIPLPPLRDM QDMVKVLFLL STDKKRYPDG RHRTLDYFRV AVEMFVTEVR QEYKRQYQQA QRNGRAMQRF TWKNSGELAI CFACCCDNVK LLYDSLQPGP LKPLWDAFVG QLPPMLIVQS RVPETMLSSQ TYHTKYMDWV KGGTRYGGEQ STANVRFPSV ADRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atcttactca cttaagcctc gatcttgtac cccgcgccac tccggaggta tgtttcaacc tttacgcgac ggtctgccac ggaggggaag cggacattcg cggtcgactg ctcgccaccg taacgtgtgc cgcccttcac ccagtccatg tacttggtgt ggtacgtttg ggatgacagc atcgtctccg gcactcggct ctgtacgatg agcatgggtg ggagctggcc gacaaaggcg tcccagagcg gcttgagggg gccaggttga aggctgtcgt acagaagctt cacattatcg cagcagcagg caaagcaaat cgccagttcg cccgagtttt tccacgtgaa ccgctgcatc gcacggccgt tgcgctgcgc ctgctggtac tgccgtttgt actcctgtcg cacttccgtc acaaacatct ccaccgcgac acggaagtag tccagcgtgc ggtgacgccc atccggatag cgtttcttat cggtgctcaa gagaaacaga accttgacca tgtcctgcat gtctctgaga ggtggaagcg ggatgtactc cttgtctggc cgctcgaagc gcagctcggg attcagctgc acatgccgct gctgttgtag catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHLQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMVKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYKRQYQQAQ RNGRAMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LPPMLIVQSR VPETMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctacaac agcagcggca tgtgcagctg aatcccgagc tgcgcttcga gcggccagac aaggagtaca tcccgcttcc acctctcaga gacatgcagg acatggtcaa ggttctgttt ctcttgagca ccgataagaa acgctatccg gatgggcgtc accgcacgct ggactacttc cgtgtcgcgg tggagatgtt tgtgacggaa gtgcgacagg agtacaaacg gcagtaccag caggcgcagc gcaacggccg tgcgatgcag cggttcacgt ggaaaaactc gggcgaactg gcgatttgct ttgcctgctg ctgcgataat gtgaagcttc tgtacgacag ccttcaacct ggccccctca agccgctctg ggacgccttt gtcggccagc tcccacccat gctcatcgta cagagccgag tgccggagac gatgctgtca tcccaaacgt accacaccaa gtacatggac tgggtgaagg gcggcacacg ttacggtggc gagcagtcga ccgcgaatgt ccgcttcccc tccgtggcag accgtcgcgt aaaggttgaa acatacctcc ggagtggcgc ggggtacaag atcgaggctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LemaA.19501.a.B4.PW38195
PURIFICATION DATe: 2/28/2017
CONCENTRATION: 25mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML QQQRHVQLNP ELRFERPDKE YIPLPPLRDM QDMVKVLFLL STDKKRYPDG RHRTLDYFRV AVEMFVTEVR QEYKRQYQQA QRNGRAMQRF TWKNSGELAI CFACCCDNVK LLYDSLQPGP LKPLWDAFVG QLPPMLIVQS RVPETMLSSQ TYHTKYMDWV KGGTRYGGEQ STANVRFPSV ADRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atcttactca cttaagcctc gatcttgtac cccgcgccac tccggaggta tgtttcaacc tttacgcgac ggtctgccac ggaggggaag cggacattcg cggtcgactg ctcgccaccg taacgtgtgc cgcccttcac ccagtccatg tacttggtgt ggtacgtttg ggatgacagc atcgtctccg gcactcggct ctgtacgatg agcatgggtg ggagctggcc gacaaaggcg tcccagagcg gcttgagggg gccaggttga aggctgtcgt acagaagctt cacattatcg cagcagcagg caaagcaaat cgccagttcg cccgagtttt tccacgtgaa ccgctgcatc gcacggccgt tgcgctgcgc ctgctggtac tgccgtttgt actcctgtcg cacttccgtc acaaacatct ccaccgcgac acggaagtag tccagcgtgc ggtgacgccc atccggatag cgtttcttat cggtgctcaa gagaaacaga accttgacca tgtcctgcat gtctctgaga ggtggaagcg ggatgtactc cttgtctggc cgctcgaagc gcagctcggg attcagctgc acatgccgct gctgttgtag catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHLQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMVKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYKRQYQQAQ RNGRAMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LPPMLIVQSR VPETMLSSQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTACAAC AGCAGCGGCA TGTGCAGCTG AATCCCGAGC TGCGCTTCGA GCGGCCAGAC AAGGAGTACA TCCCGCTTCC ACCTCTCAGA GACATGCAGG ACATGGTCAA GGTTCTGTTT CTCTTGAGCA CCGATAAGAA ACGCTATCCG GATGGGCGTC ACCGCACGCT GGACTACTTC CGTGTCGCGG TGGAGATGTT TGTGACGGAA GTGCGACAGG AGTACAAACG GCAGTACCAG CAGGCGCAGC GCAACGGCCG TGCGATGCAG CGGTTCACGT GGAAAAACTC GGGCGAACTG GCGATTTGCT TTGCCTGCTG CTGCGATAAT GTGAAGCTTC TGTACGACAG CCTTCAACCT GGCCCCCTCA AGCCGCTCTG GGACGCCTTT GTCGGCCAGC TCCCACCCAT GCTCATCGTA CAGAGCCGAG TGCCGGAGAC GATGCTGTCA TCCCAAACGT ACCACACCAA GTACATGGAC TGGGTGAAGG GCGGCACACG TTACGGTGGC GAGCAGTCGA CCGCGAATGT CCGCTTCCCC TCCGTGGCAG ACCGTCGCGT AAAGGTTGAA ACATACCTCC GGAGTGGCGC GGGGTACAAG ATCGAGGCTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT