SSGCID: Target Page

LemaA.18889.a

alanyl-tRNA synthetase, putative

CENTER ID:	LemaA.18889.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.18889.a.B1.GE42807	full length		1	962

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.22.1540
UniProt:	Q4QBJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFTEWPVSR VRQEFVDYFK KQGHTFVPSS PVCPHDDPTL LFINAGMNQF KALFLGTADP NTDFGRLKRA ANSQMCIRAG GKHNDLEDVG RDTYHHTFFE MLGSWSFGDY FKKEAIQWAW ELLTEVYKLP KDQLYVTYFE GDESNGLDPD LEAKELWGQL LPESQIVPGN AKDNFWEMGD TGPCGPCSEV HFDRIGRRNA ASLVNKDDPM VVEIWNLVFM QFERRAGGFL TPLPQKHIDT GMGLERLTSI LQGVKSNYDT DAWIPLFEAI QTATGYPKSY AEIRNDPDSD ALVAYRVVAD HIRCLTSAVG DGVMPDSVGR GFVLRRIIRR AVRYGVQFLG AKPGFFTQLV DSVCTSLGPF FPHLKEPRNI QRIKAVLADE EQSFAKTWEM GLKHFNHAVN ECRANSSKVI SGSEAFVLHD RYGFPVDLTC LLAEKDGMTV DLEGFNTEMK ESKVSAGRVA ATKTFIDAYQ LEELKTRGIP QTNDAAKYVW EDYTGDVLAI FDKKNSKFIS LLEPGNDLGA EGVGIILSTT NFYAESGGQV YDTGRLVAAS DSVFEVKKAY NVAGYVVHVG NLSKYSASPI PSSAVVQLQV DYERRLPIAA NHTTTHQLNW VLRRVLGEKP DNFTEVQQKG SLVTDEMLRF DFSYNTKLSN EDLVRVEQLL NEKIQAGLLV YREEVPLEAA SKINGLRQMF GEKYPDPVSV ISIGTPINEM LANPEKEEWR DYAVEFCGGT HLKNLKEAEK AVIISEEALM KGVRRVVVAT RLEADKVIKA GAELKRQYDE LMKKEATATS VKALSVLNKN VGDSCIPLVL KNEMRENIDA AIKRMNATLK SQAAEARDKA AEAGKCLGAS YDAAAAPFLV HQMTEFGAER EALQAFSDGF SSTVSGPVGV FLIGSDNEKA LAIVSMPAAF VEKKMSAVAW AKSSIGKGGG KPNAAQSGLP AKDVAAAVAK AMEEAERMKA SL

NT Sequence

atgtcgttca cggagtggcc tgtgagtcgg gttcgccagg agttcgtcga ctacttcaag aagcagggtc acacatttgt gcccagcagc cccgtctgcc ctcacgatga cccgaccctg ctgttcatca atgcgggcat gaatcagttc aaggcgctct ttttggggac cgctgacccc aacaccgact ttggccgtct gaagcgcgct gccaactcgc agatgtgcat tcgcgctggt ggcaagcaca acgacttgga ggatgtcggt cgcgacacgt accaccacac ctttttcgag atgctaggat catggtcctt tggcgactac ttcaagaagg aggccatcca gtgggcctgg gagctgctga cagaggtgta caagctgccc aaggatcagc tctacgtgac ctactttgag ggggacgaaa gtaacgggct ggatccggat ctggaggcga aggagctgtg gggccagctt ctcccagaga gccagatcgt ccccggcaac gccaaggaca acttttggga gatgggtgac actggcccgt gcggtccgtg ctcggaggtt cactttgacc gtatcggtag gcgcaacgct gctagcctcg tcaacaagga cgatccgatg gtggtggaga tatggaatct ggtgtttatg cagttcgagc gtcgcgccgg tggcttcctc accccactgc cgcagaagca catagacacc ggcatgggtc tagagcgcct cacttctatt ctgcagggtg ttaagtccaa ctacgacaca gatgcgtgga ttcctctctt tgaggccatc cagactgcga ccggctaccc caagtcgtac gccgagatcc gcaacgaccc cgacagcgat gccttggtgg cctaccgcgt tgtcgccgat cacattcggt gcctgacgtc ggccgtgggc gacggcgtca tgcccgactc cgtcggccgc ggcttcgtgc tgcgtcgaat tatccgccgc gctgtgcgct acggtgtgca gttcctcggt gccaagcctg gcttcttcac gcagctggtc gacagtgtgt gcacctcgtt gggcccattc ttcccgcacc tgaaggaacc ccgcaatatc cagcgcatca aggcggtgct ggccgacgag gagcagtcgt ttgccaagac atgggagatg gggctgaagc attttaacca cgccgtgaat gagtgtcgag caaacagctc gaaggtcatt agcggctccg aggcttttgt gcttcatgac cggtacgggt tcccggtgga tctgacgtgc ctgctggctg agaaggacgg catgacagtg gacttggagg gcttcaacac ggagatgaag gagagcaagg ttagcgcagg ccgtgtggcg gccaccaaga ccttcattga cgcataccag ctcgaggagc tcaagacgcg cggtataccg cagaccaatg acgctgcgaa gtatgtctgg gaggactaca ccggcgacgt actcgctatc ttcgacaaga agaacagcaa gttcatctct cttctggagc ctggcaacga ccttggagcc gagggggtcg gtatcatcct gagcaccacg aacttttacg ccgagtctgg cggccaggtg tacgacaccg gccgcctggt cgcggctagc gacagtgtgt tcgaggtgaa gaaggcgtac aacgtggccg gctacgtggt gcacgtgggc aacctcagca agtactctgc ctccccgatc ccctcgtctg cggtggtgca gctgcaggtg gactacgagc gccgcctgcc gatcgcggca aaccatacca cgacccacca gctgaactgg gtactgcgcc gtgtgctggg ggagaagccg gataacttca cagaggtgca gcagaagggc tccctcgtga cggacgagat gctccgcttc gatttcagct ataacacaaa gctgtcgaac gaggaccttg tgcgcgtgga gcagctactg aacgagaaga ttcaggcggg cctgctcgtc taccgtgagg aggtgcccct ggaggctgct agcaagatca acggtcttcg ccagatgttt ggcgagaagt accccgaccc cgtcagcgtc atctccatcg gcaccccaat caacgaaatg ctggcgaacc ccgagaaaga ggagtggcgc gattacgccg tcgagttctg cggcggcacg cacctgaaaa acctcaagga ggccgaaaag gcggtaataa tctcagagga ggccctcatg aagggcgtgc gccgcgtggt cgtggccacc aggttggagg ctgacaaggt catcaaggct ggtgctgagc tgaagagaca atacgatgag ctgatgaaaa aggaggcgac ggcgaccagc gtcaaggcgc tttcagtgct gaacaagaat gtaggtgaca gctgcattcc gctggtgctc aagaacgaga tgcgcgaaaa catcgacgcg gccatcaagc ggatgaacgc gaccctcaag tcgcaggctg cggaggcacg ggataaagcc gcagaggcag gcaagtgcct cggcgcgtcg tacgacgctg ctgccgcgcc attcctcgtt caccagatga ccgaattcgg tgcggagcgc gaggcactgc aggcgttttc cgacggcttc tccagcaccg tgagcggacc ggtcggcgtc ttcctcatcg gcagcgacaa cgagaaggcg ctcgcgattg tgtccatgcc ggccgctttt gtggagaaga agatgagcgc tgtcgcgtgg gccaagtctt ccattggcaa gggtggcggt aagcccaacg ccgctcagtc cggtctcccc gccaaggacg tcgctgcggc tgtggcgaag gccatggaag aagcagagag gatgaaggcg tctctgtag

Details for LemaA.18889.a.B1.GE42807

HARVESTED ON:	1/9/2019
SEQUENCED ON:	1/15/2019
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS FTEWPVSRVR QEFVDYFKKQ GHTFVPSSPV CPHDDPTLLF INAGMNQFKA LFLGTADPNT DFGRLKRAAN SQMCIRAGGK HNDLEDVGRD TYHHTFFEML GSWSFGDYFK KEAIQWAWEL LTEVYKLPKD QLYVTYFEGD ESNGLDPDLE AKELWGQLLP ESQIVPGNAK DNFWEMGDTG PCGPCSEVHF DRIGRRNAAS LVNKDDPMVV EIWNLVFMQF ERRAGGFLTP LPQKHIDTGM GLERLTSILQ GVKSNYDTDA WIPLFEAIQT ATGYPKSYAE IRNDPDSDAL VAYRVVADHI RCLTSAVGDG VMPDSVGRGF VLRRIIRRAV RYGVQFLGAK PGFFTQLVDS VCTSLGPFFP HLKEPRNIQR IKAVLADEEQ SFAKTWEMGL KHFNHAVNEC RANSSKVISG SEAFVLHDRY GFPVDLTCLL AEKDGMTVDL EGFNTEMKES KVSAGRVAAT KTFIDAYQLE ELKTRGIPQT NDAAKYVWED YTGDVLAIFD KKNSKFISLL EPGNDLGAEG VGIILSTTNF YAESGGQVYD TGRLVAASDS VFEVKKAYNV AGYVVHVGNL SKYSASPIPS SAVVQLQVDY ERRLPIAANH TTTHQLNWVL RRVLGEKPDN FTEVQQKGSL VTDEMLRFDF SYNTKLSNED LVRVEQLLNE KIQAGLLVYR EEVPLEAASK INGLRQMFGE KYPDPVSVIS IGTPINEMLA NPEKEEWRDY AVEFCGGTHL KNLKEAEKAV IISEEALMKG VRRVVVATRL EADKVIKAGA ELKRQYDELM KKEATATSVK ALSVLNKNVG DSCIPLVLKN EMRENIDAAI KRMNATLKSQ AAEARDKAAE AGKCLGASYD AAAAPFLVHQ MTEFGAEREA LQAFSDGFSS TVSGPVGVFL IGSDNEKALA IVSMPAAFVE KKMSAVAWAK SSIGKGGGKP NAAQSGLPAK DVAAAVAKAM EEAERMKASL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt tcacggagtg gcctgtgagt cgggttcgcc aggagttcgt cgactacttc aagaagcagg gtcacacatt tgtgcccagc agccccgtct gccctcacga tgacccgacc ctgctgttca tcaatgcggg catgaatcag ttcaaggcgc tctttttggg gaccgctgac cccaacaccg actttggccg tctgaagcgc gctgccaact cgcagatgtg cattcgcgct ggtggcaagc acaacgactt ggaggatgtc ggtcgcgaca cgtaccacca cacctttttc gagatgctag gatcatggtc ctttggcgac tacttcaaga aggaggccat ccagtgggcc tgggagctgc tgacagaggt gtacaagctg cccaaggatc agctctacgt gacctacttt gagggggacg aaagtaacgg gctggatccg gatctggagg cgaaggagct gtggggccag cttctcccag agagccagat cgtccccggc aacgccaagg acaacttttg ggagatgggt gacactggcc cgtgcggtcc gtgctcggag gttcactttg accgtatcgg taggcgcaac gctgctagcc tcgtcaacaa ggacgatccg atggtggtgg agatatggaa tctggtgttt atgcagttcg agcgtcgcgc cggtggcttc ctcaccccac tgccgcagaa gcacatagac accggcatgg gtctagagcg cctcacttct attctgcagg gtgttaagtc caactacgac acagatgcgt ggattcctct ctttgaggcc atccagactg cgaccggcta ccccaagtcg tacgccgaga tccgcaacga ccccgacagc gatgccttgg tggcctaccg cgttgtcgcc gatcacattc ggtgcctgac gtcggccgtg ggcgacggcg tcatgcccga ctccgtcggc cgcggcttcg tgctgcgtcg aattatccgc cgcgctgtgc gctacggtgt gcagttcctc ggtgccaagc ctggcttctt cacgcagctg gtcgacagtg tgtgcacctc gttgggccca ttcttcccgc acctgaagga accccgcaat atccagcgca tcaaggcggt gctggccgac gaggagcagt cgtttgccaa gacatgggag atggggctga agcattttaa ccacgccgtg aatgagtgtc gagcaaacag ctcgaaggtc attagcggct ccgaggcttt tgtgcttcat gaccggtacg ggttcccggt ggatctgacg tgcctgctgg ctgagaagga cggcatgaca gtggacttgg agggcttcaa cacggagatg aaggagagca aggttagcgc aggccgtgtg gcggccacca agaccttcat tgacgcatac cagctcgagg agctcaagac gcgcggtata ccgcagacca atgacgctgc gaagtatgtc tgggaggact acaccggcga cgtactcgct atcttcgaca agaagaacag caagttcatc tctcttctgg agcctggcaa cgaccttgga gccgaggggg tcggtatcat cctgagcacc acgaactttt acgccgagtc tggcggccag gtgtacgaca ccggccgcct ggtcgcggct agcgacagtg tgttcgaggt gaagaaggcg tacaacgtgg ccggctacgt ggtgcacgtg ggcaacctca gcaagtactc tgcctccccg atcccctcgt ctgcggtggt gcagctgcag gtggactacg agcgccgcct gccgatcgcg gcaaaccata ccacgaccca ccagctgaac tgggtactgc gccgtgtgct gggggagaag ccggataact tcacagaggt gcagcagaag ggctccctcg tgacggacga gatgctccgc ttcgatttca gctataacac aaagctgtcg aacgaggacc ttgtgcgcgt ggagcagcta ctgaacgaga agattcaggc gggcctgctc gtctaccgtg aggaggtgcc cctggaggct gctagcaaga tcaacggtct tcgccagatg tttggcgaga agtaccccga ccccgtcagc gtcatctcca tcggcacccc aatcaacgaa atgctggcga accccgagaa agaggagtgg cgcgattacg ccgtcgagtt ctgcggcggc acgcacctga aaaacctcaa ggaggccgaa aaggcggtaa taatctcaga ggaggccctc atgaagggcg tgcgccgcgt ggtcgtggcc accaggttgg aggctgacaa ggtcatcaag gctggtgctg agctgaagag acaatacgat gagctgatga aaaaggaggc gacggcgacc agcgtcaagg cgctttcagt gctgaacaag aatgtaggtg acagctgcat tccgctggtg ctcaagaacg agatgcgcga aaacatcgac gcggccatca agcggatgaa cgcgaccctc aagtcgcagg ctgcggaggc acgggataaa gccgcagagg caggcaagtg cctcggcgcg tcgtacgacg ctgctgccgc gccattcctc gttcaccaga tgaccgaatt cggtgcggag cgcgaggcac tgcaggcgtt ttccgacggc ttctccagca ccgtgagcgg accggtcggc gtcttcctca tcggcagcga caacgagaag gcgctcgcga ttgtgtccat gccggccgct tttgtggaga agaagatgag cgctgtcgcg tgggccaagt cttccattgg caagggtggc ggtaagccca acgccgctca gtccggtctc cccgccaagg acgtcgctgc ggctgtggcg aaggccatgg aagaagcaga gaggatgaag gcgtctctgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat