SSGCID: Target Page

LemaA.18849.a

valyl-tRNA synthetase, putative

CENTER ID:	LemaA.18849.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.18849.a.B1.GE42806	full length		1	967

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.18849.a.B1.PW38669	full length		1	967

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.30.3130
UniProt:	Q4Q6X7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAATYDPARV EADWYPWWEK SGFFRPASDH NSETATKPFV IIAPPPNVTG YLHLGHALTG AVQDTLIRFH RMKGDNTLYL PGTDHAGIAT QVVVERRVMK EEGKSRHDLG RDEFMKRLWE FKKSHAGMIT EQFRRIGLSL DWTRERFTMD EQSSAAVVEA FVRLHEDGLV HRDTRLVNWC CALQSAISDL EVEFVEVPKT SKMTIPLYDR KVDMGSLTHV AYKLVDSDDE LVIATTRPET LLGDTAVAIH PDDERYKKFH GKFLKCPFRD DIIPIVLDAT LVDMNFGTGA VKITPAHDPN DFESGKRHNL QQLVMMDLKG YVTMEPFKGM HRFDCRREIV KKLEEMGLLR GVEPYEYRVG RCERTGDIVE PMLMPQWFID CSDMARRAVE AVRNGDLCLY PPSHEAVWYH WLENIKPWCV SRQLWWGHRI PAYKVVGSLP KDVDPWVVAR NLQEAHAKAK KKFGLTDEQV AEASFEQDPD VLDTWFSSGL WPFSTLGWPT DSDDMKRFFP NSLMETGHDI LFFWVARMVM LSLHFTNKLP YKEVFLHAMV RDKNGEKMSK SKGNVIDPLY VIHGVSLQTL HDTVRSGNLS DKEVEKAIKQ QKEFFPEGIP ECGSDALRFG LLSYTQSGRS VNLDIQRVVA YRQFCNKLWN VVRYVLYHAL GTDYVPSKQQ FSPAEDAAML PLECRWILSR LDAAIAETTQ GMSEGLYDFA LATGAVYRFW LHELCDVFLE LTKPTIQKGG EKQQLVQDVL LHVVEKALRL LHPMMPFLTE ELWHRLPNYS SFGSESIMLA KYPTPSGWSS AASDSAMSII LDVVHSVRSI KASYSLTNKH KPDVWVTAHT AELQELFAVG KMMISTLGVV GEVTVVSPAE EAAAVPKGCG FSLVTKEVGV NMMLMGFIDV GKEVAKLEKQ LDGLTKQIEG MKKKMSIPNY ETKVPAEIRV ANAEKLSTLE AQSAQLRDGI EKMSSLK

NT Sequence

atggcggcca cgtacgatcc ggcccgggtg gaggcggact ggtatccgtg gtgggagaag tctggcttct tcagacccgc gtcagaccac aattctgaga cggcgaccaa gccgttcgtg attatcgccc caccaccaaa cgtcacgggc tacctgcacc tgggccatgc cctcacgggc gctgtgcagg acaccctcat ccgcttccac cgcatgaagg gcgacaacac cctctacttg cccggcaccg accacgccgg catcgccacc caggttgtcg tggagcgccg cgtcatgaag gaggaaggca aaagccggca cgacctcggc cgcgacgagt tcatgaagcg cttgtgggaa ttcaagaaga gccacgctgg catgatcacg gagcagttcc gcaggatcgg cttgtcgctg gactggacgc gcgagcgctt tacgatggac gaacagtcgt ctgcggctgt tgtagaggcg tttgtgcgcc tgcacgagga cggcctggtg caccgcgaca cacggctggt gaactggtgc tgcgcgctgc agagcgccat ctctgacctc gaggtggagt ttgttgaagt gcccaagaca tccaagatga ccattcctct ctatgaccgc aaagtcgaca tgggcagcct cactcacgtc gcctacaagc tggtggacag cgatgacgag ctggtgattg cgacgacgcg tcctgagacg ctgctgggcg atacggccgt cgccatccac cccgacgacg agcgctacaa aaagttccat ggtaagttcc tcaagtgccc gttccgggac gacatcatcc cgatcgtgct ggacgcaacg ctggtcgaca tgaactttgg cactggcgcg gtgaaaatta ccccagctca cgacccaaac gactttgagt ccggcaagcg ccacaacctg cagcagcttg tcatgatgga cctgaagggc tacgtgacga tggagccatt caagggcatg caccgcttcg actgccgccg cgagatcgtg aagaagctgg aggagatggg gctgctgcgc ggcgtggagc cgtacgagta ccgcgtgggc cggtgcgagc gcactggcga cattgtggag ccgatgctga tgccgcagtg gttcatcgac tgctccgata tggcccgccg cgccgtcgag gcggtgcgca acggcgacct gtgcctgtac ccgccctcgc acgaggccgt ctggtaccac tggctggaga acatcaaacc gtggtgcgtg tcgcgtcagc tctggtgggg ccaccgcatt cctgcctaca aagtcgtcgg ctcgctgccg aaagacgtgg acccatgggt ggtcgcccgc aatctacagg aggcgcacgc gaaggcgaag aagaagttcg gcctgaccga cgagcaggtc gccgaggcct cgtttgagca ggatccggat gtgctggaca cctggttcag ctccggcctc tggcccttct ccacgctcgg atggcccacc gactcggacg atatgaagcg cttcttcccg aactcgctga tggagaccgg ccacgacatc ctgttcttct gggtggctcg catggtaatg ctttcgctgc acttcacaaa caaactgccg tacaaggagg tgtttctgca cgcaatggtg cgcgacaaga acggcgagaa gatgtccaag tcgaagggca acgtcatcga cccactctat gtcatccacg gcgtctcgct gcagaccctc cacgacaccg tgcgcagcgg caacctgagc gacaaggagg tggagaaggc catcaagcag cagaaggagt tcttcccaga aggcattcca gagtgcggta gcgatgccct gcgcttcggt cttctctcct acacccagtc tggtcgcagc gtgaacctcg acatccaacg cgtcgtggcc taccgccagt tctgcaacaa gctgtggaac gtggtgcgct acgtgctgta tcacgccctg ggcaccgact acgtgcccag caagcagcag ttcagccccg ccgaggacgc cgccatgctg ccgctcgagt gtcgctggat tctctcgcgc ctcgacgccg ccatcgcgga gaccacgcag ggcatgtcgg agggcctcta cgactttgcg ctggccaccg gtgccgtcta ccgcttctgg ctgcacgagc tgtgcgacgt gttccttgag ttgacgaagc cgaccattca gaagggcgga gagaagcagc agctggtgca agacgtgctg ttgcacgtcg tcgagaaggc acttcggctg ctacacccga tgatgccgtt tctcacggag gagctttggc accgtctgcc caactacagc agcttcggca gcgagtcgat catgctcgcc aagtacccga caccgagcgg gtggtcgagc gcggcgtcag atagcgccat gtcgatcatt ctcgacgttg tgcacagcgt acgttccatc aaggcctcgt actcgcttac gaacaagcac aagccagacg tgtgggtgac ggcgcacacg gcggagctgc aggagctgtt cgcggtggga aagatgatga tctcaaccct cggtgtcgta ggcgaggtga ctgtcgtgtc tccggccgag gaggcagcgg cggtgccgaa gggctgcggc ttttccttgg tgacaaagga ggtgggcgtg aacatgatgc tcatgggttt catcgacgtc gggaaggagg tggcgaagct ggagaagcag ctggacggcc tcacgaagca gattgaggga atgaagaaga agatgtccat ccctaattac gaaactaagg tgccggccga gatccgcgtg gccaacgccg aaaagctcag cacgctagag gcgcagagcg cgcagctgcg cgatggcatc gagaagatga gcagcctcaa gtga

Details for LemaA.18849.a.B1.GE42806

HARVESTED ON:	1/9/2019
SEQUENCED ON:	1/15/2019
EXPECTED MW:	111kDa
OBSERVED MW:	111kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA ATYDPARVEA DWYPWWEKSG FFRPASDHNS ETATKPFVII APPPNVTGYL HLGHALTGAV QDTLIRFHRM KGDNTLYLPG TDHAGIATQV VVERRVMKEE GKSRHDLGRD EFMKRLWEFK KSHAGMITEQ FRRIGLSLDW TRERFTMDEQ SSAAVVEAFV RLHEDGLVHR DTRLVNWCCA LQSAISDLEV EFVEVPKTSK MTIPLYDRKV DMGSLTHVAY KLVDSDDELV IATTRPETLL GDTAVAIHPD DERYKKFHGK FLKCPFRDDI IPIVLDATLV DMNFGTGAVK ITPAHDPNDF ESGKRHNLQQ LVMMDLKGYV TMEPFKGMHR FDCRREIVKK LEEMGLLRGV EPYEYRVGRC ERTGDIVEPM LMPQWFIDCS DMARRAVEAV RNGDLCLYPP SHEAVWYHWL ENIKPWCVSR QLWWGHRIPA YKVVGSLPKD VDPWVVARNL QEAHAKAKKK FGLTDEQVAE ASFEQDPDVL DTWFSSGLWP FSTLGWPTDS DDMKRFFPNS LMETGHDILF FWVARMVMLS LHFTNKLPYK EVFLHAMVRD KNGEKMSKSK GNVIDPLYVI HGVSLQTLHD TVRSGNLSDK EVEKAIKQQK EFFPEGIPEC GSDALRFGLL SYTQSGRSVN LDIQRVVAYR QFCNKLWNVV RYVLYHALGT DYVPSKQQFS PAEDAAMLPL ECRWILSRLD AAIAETTQGM SEGLYDFALA TGAVYRFWLH ELCDVFLELT KPTIQKGGEK QQLVQDVLLH VVEKALRLLH PMMPFLTEEL WHRLPNYSSF GSESIMLAKY PTPSGWSSAA SDSAMSIILD VVHSVRSIKA SYSLTNKHKP DVWVTAHTAE LQELFAVGKM MISTLGVVGE VTVVSPAEEA AAVPKGCGFS LVTKEVGVNM MLMGFIDVGK EVAKLEKQLD GLTKQIEGMK KKMSIPNYET KVPAEIRVAN AEKLSTLEAQ SAQLRDGIEK MSSLK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg ccacgtacga tccggcccgg gtggaggcgg actggtatcc gtggtgggag aagtctggct tcttcagacc cgcgtcagac cacaattctg agacggcgac caagccgttc gtgattatcg ccccaccacc aaacgtcacg ggctacctgc acctgggcca tgccctcacg ggcgctgtgc aggacaccct catccgcttc caccgcatga agggcgacaa caccctctac ttgcccggca ccgaccacgc cggcatcgcc acccaggttg tcgtggagcg ccgcgtcatg aaggaggaag gcaaaagccg gcacgacctc ggccgcgacg agttcatgaa gcgcttgtgg gaattcaaga agagccacgc tggcatgatc acggagcagt tccgcaggat cggcttgtcg ctggactgga cgcgcgagcg ctttacgatg gacgaacagt cgtctgcggc tgttgtagag gcgtttgtgc gcctgcacga ggacggcctg gtgcaccgcg acacacggct ggtgaactgg tgctgcgcgc tgcagagcgc catctctgac ctcgaggtgg agtttgttga agtgcccaag acatccaaga tgaccattcc tctctatgac cgcaaagtcg acatgggcag cctcactcac gtcgcctaca agctggtgga cagcgatgac gagctggtga ttgcgacgac gcgtcctgag acgctgctgg gcgatacggc cgtcgccatc caccccgacg acgagcgcta caaaaagttc catggtaagt tcctcaagtg cccgttccgg gacgacatca tcccgatcgt gctggacgca acgctggtcg acatgaactt tggcactggc gcggtgaaaa ttaccccagc tcacgaccca aacgactttg agtccggcaa gcgccacaac ctgcagcagc ttgtcatgat ggacctgaag ggctacgtga cgatggagcc attcaagggc atgcaccgct tcgactgccg ccgcgagatc gtgaagaagc tggaggagat ggggctgctg cgcggcgtgg agccgtacga gtaccgcgtg ggccggtgcg agcgcactgg cgacattgtg gagccgatgc tgatgccgca gtggttcatc gactgctccg atatggcccg ccgcgccgtc gaggcggtgc gcaacggcga cctgtgcctg tacccgccct cgcacgaggc cgtctggtac cactggctgg agaacatcaa accgtggtgc gtgtcgcgtc agctctggtg gggccaccgc attcctgcct acaaagtcgt cggctcgctg ccgaaagacg tggacccatg ggtggtcgcc cgcaatctac aggaggcgca cgcgaaggcg aagaagaagt tcggcctgac cgacgagcag gtcgccgagg cctcgtttga gcaggatccg gatgtgctgg acacctggtt cagctccggc ctctggccct tctccacgct cggatggccc accgactcgg acgatatgaa gcgcttcttc ccgaactcgc tgatggagac cggccacgac atcctgttct tctgggtggc tcgcatggta atgctttcgc tgcacttcac aaacaaactg ccgtacaagg aggtgtttct gcacgcaatg gtgcgcgaca agaacggcga gaagatgtcc aagtcgaagg gcaacgtcat cgacccactc tatgtcatcc acggcgtctc gctgcagacc ctccacgaca ccgtgcgcag cggcaacctg agcgacaagg aggtggagaa ggccatcaag cagcagaagg agttcttccc agaaggcatt ccagagtgcg gtagcgatgc cctgcgcttc ggtcttctct cctacaccca gtctggtcgc agcgtgaacc tcgacatcca acgcgtcgtg gcctaccgcc agttctgcaa caagctgtgg aacgtggtgc gctacgtgct gtatcacgcc ctgggcaccg actacgtgcc cagcaagcag cagttcagcc ccgccgagga cgccgccatg ctgccgctcg agtgtcgctg gattctctcg cgcctcgacg ccgccatcgc ggagaccacg cagggcatgt cggagggcct ctacgacttt gcgctggcca ccggtgccgt ctaccgcttc tggctgcacg agctgtgcga cgtgttcctt gagttgacga agccgaccat tcagaagggc ggagagaagc agcagctggt gcaagacgtg ctgttgcacg tcgtcgagaa ggcacttcgg ctgctacacc cgatgatgcc gtttctcacg gaggagcttt ggcaccgtct gcccaactac agcagcttcg gcagcgagtc gatcatgctc gccaagtacc cgacaccgag cgggtggtcg agcgcggcgt cagatagcgc catgtcgatc attctcgacg ttgtgcacag cgtacgttcc atcaaggcct cgtactcgct tacgaacaag cacaagccag acgtgtgggt gacggcgcac acggcggagc tgcaggagct gttcgcggtg ggaaagatga tgatctcaac cctcggtgtc gtaggcgagg tgactgtcgt gtctccggcc gaggaggcag cggcggtgcc gaagggctgc ggcttttcct tggtgacaaa ggaggtgggc gtgaacatga tgctcatggg tttcatcgac gtcgggaagg aggtggcgaa gctggagaag cagctggacg gcctcacgaa gcagattgag ggaatgaaga agaagatgtc catccctaat tacgaaacta aggtgccggc cgagatccgc gtggccaacg ccgaaaagct cagcacgcta gaggcgcaga gcgcgcagct gcgcgatggc atcgagaaga tgagcagcct caagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LemaA.18849.a.B1.PW38669

PURIFICATION DATe:	7/23/2019
CONCENTRATION:	33mg/ml
OBSERVED MW:	105kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG CCACGTACGA TCCGGCCCGG GTGGAGGCGG ACTGGTATCC GTGGTGGGAG AAGTCTGGCT TCTTCAGACC CGCGTCAGAC CACAATTCTG AGACGGCGAC CAAGCCGTTC GTGATTATCG CCCCACCACC AAACGTCACG GGCTACCTGC ACCTGGGCCA TGCCCTCACG GGCGCTGTGC AGGACACCCT CATCCGCTTC CACCGCATGA AGGGCGACAA CACCCTCTAC TTGCCCGGCA CCGACCACGC CGGCATCGCC ACCCAGGTTG TCGTGGAGCG CCGCGTCATG AAGGAGGAAG GCAAAAGCCG GCACGACCTC GGCCGCGACG AGTTCATGAA GCGCTTGTGG GAATTCAAGA AGAGCCACGC TGGCATGATC ACGGAGCAGT TCCGCAGGAT CGGCTTGTCG CTGGACTGGA CGCGCGAGCG CTTTACGATG GACGAACAGT CGTCTGCGGC TGTTGTAGAG GCGTTTGTGC GCCTGCACGA GGACGGCCTG GTGCACCGCG ACACACGGCT GGTGAACTGG TGCTGCGCGC TGCAGAGCGC CATCTCTGAC CTCGAGGTGG AGTTTGTTGA AGTGCCCAAG ACATCCAAGA TGACCATTCC TCTCTATGAC CGCAAAGTCG ACATGGGCAG CCTCACTCAC GTCGCCTACA AGCTGGTGGA CAGCGATGAC GAGCTGGTGA TTGCGACGAC GCGTCCTGAG ACGCTGCTGG GCGATACGGC CGTCGCCATC CACCCCGACG ACGAGCGCTA CAAAAAGTTC CATGGTAAGT TCCTCAAGTG CCCGTTCCGG GACGACATCA TCCCGATCGT GCTGGACGCA ACGCTGGTCG ACATGAACTT TGGCACTGGC GCGGTGAAAA TTACCCCAGC TCACGACCCA AACGACTTTG AGTCCGGCAA GCGCCACAAC CTGCAGCAGC TTGTCATGAT GGACCTGAAG GGCTACGTGA CGATGGAGCC ATTCAAGGGC ATGCACCGCT TCGACTGCCG CCGCGAGATC GTGAAGAAGC TGGAGGAGAT GGGGCTGCTG CGCGGCGTGG AGCCGTACGA GTACCGCGTG GGCCGGTGCG AGCGCACTGG CGACATTGTG GAGCCGATGC TGATGCCGCA GTGGTTCATC GACTGCTCCG ATATGGCCCG CCGCGCCGTC GAGGCGGTGC GCAACGGCGA CCTGTGCCTG TACCCGCCCT CGCACGAGGC CGTCTGGTAC CACTGGCTGG AGAACATCAA ACCGTGGTGC GTGTCGCGTC AGCTCTGGTG GGGCCACCGC ATTCCTGCCT ACAAAGTCGT CGGCTCGCTG CCGAAAGACG TGGACCCATG GGTGGTCGCC CGCAATCTAC AGGAGGCGCA CGCGAAGGCG AAGAAGAAGT TCGGCCTGAC CGACGAGCAG GTCGCCGAGG CCTCGTTTGA GCAGGATCCG GATGTGCTGG ACACCTGGTT CAGCTCCGGC CTCTGGCCCT TCTCCACGCT CGGATGGCCC ACCGACTCGG ACGATATGAA GCGCTTCTTC CCGAACTCGC TGATGGAGAC CGGCCACGAC ATCCTGTTCT TCTGGGTGGC TCGCATGGTA ATGCTTTCGC TGCACTTCAC AAACAAACTG CCGTACAAGG AGGTGTTTCT GCACGCAATG GTGCGCGACA AGAACGGCGA GAAGATGTCC AAGTCGAAGG GCAACGTCAT CGACCCACTC TATGTCATCC ACGGCGTCTC GCTGCAGACC CTCCACGACA CCGTGCGCAG CGGCAACCTG AGCGACAAGG AGGTGGAGAA GGCCATCAAG CAGCAGAAGG AGTTCTTCCC AGAAGGCATT CCAGAGTGCG GTAGCGATGC CCTGCGCTTC GGTCTTCTCT CCTACACCCA GTCTGGTCGC AGCGTGAACC TCGACATCCA ACGCGTCGTG GCCTACCGCC AGTTCTGCAA CAAGCTGTGG AACGTGGTGC GCTACGTGCT GTATCACGCC CTGGGCACCG ACTACGTGCC CAGCAAGCAG CAGTTCAGCC CCGCCGAGGA CGCCGCCATG CTGCCGCTCG AGTGTCGCTG GATTCTCTCG CGCCTCGACG CCGCCATCGC GGAGACCACG CAGGGCATGT CGGAGGGCCT CTACGACTTT GCGCTGGCCA CCGGTGCCGT CTACCGCTTC TGGCTGCACG AGCTGTGCGA CGTGTTCCTT GAGTTGACGA AGCCGACCAT TCAGAAGGGC GGAGAGAAGC AGCAGCTGGT GCAAGACGTG CTGTTGCACG TCGTCGAGAA GGCACTTCGG CTGCTACACC CGATGATGCC GTTTCTCACG GAGGAGCTTT GGCACCGTCT GCCCAACTAC AGCAGCTTCG GCAGCGAGTC GATCATGCTC GCCAAGTACC CGACACCGAG CGGGTGGTCG AGCGCGGCGT CAGATAGCGC CATGTCGATC ATTCTCGACG TTGTGCACAG CGTACGTTCC ATCAAGGCCT CGTACTCGCT TACGAACAAG CACAAGCCAG ACGTGTGGGT GACGGCGCAC ACGGCGGAGC TGCAGGAGCT GTTCGCGGTG GGAAAGATGA TGATCTCAAC CCTCGGTGTC GTAGGCGAGG TGACTGTCGT GTCTCCGGCC GAGGAGGCAG CGGCGGTGCC GAAGGGCTGC GGCTTTTCCT TGGTGACAAA GGAGGTGGGC GTGAACATGA TGCTCATGGG TTTCATCGAC GTCGGGAAGG AGGTGGCGAA GCTGGAGAAG CAGCTGGACG GCCTCACGAA GCAGATTGAG GGAATGAAGA AGAAGATGTC CATCCCTAAT TACGAAACTA AGGTGCCGGC CGAGATCCGC GTGGCCAACG CCGAAAAGCT CAGCACGCTA GAGGCGCAGA GCGCGCAGCT GCGCGATGGC ATCGAGAAGA TGAGCAGCCT CAAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT