LemaA.18809.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID: LemaA.18809.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18809.a.B2.GE41028 mature protein; soluble domain 19 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.30.3190
UniProt: Q9Y0F3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRSLLLACS AAKGESWASM SDTEIMKQVE NKKIAFHGLE QALAPDYDRA IAIRREIVKK KICPSPAATH PLERVPYKNY DWSSVVGQSC ENILGYVPVP VGLAGPLLLD GKEVALPMAT TEGALVASAH RGARAINLSG GCRTAVLKEG MTRAPVVEVN SFDEAITVIK FCEERFDVLR EAFESTTRFG KLLSIKCAMA GRQVHLRFSA FTGDAMGMNM ITKGCDKALQ VLQQHIPSVR VLTLSGNFCT DKKPSALNWV EGRGKSVVAE AVIKRDVVES VLKCTVDSVV SLNVTKNLRG SALAGSIGGF NAHAANIVAA LYIATGQDPA QVVESATCMT TVDKAGEDLV ISLMMPSIEV GAVGGGTGLS SQRAMLELMG CAGSNKEDPG AHSRQIARVV AGAVICGELS LLSGLAAGHL LSAHMKLNRK PPTP
NT Sequence
atgcgccgct ctctgctgct tgcctgctcc gccgccaagg gtgagagctg ggcatccatg tccgatacag agatcatgaa gcaggtggag aataagaaga ttgcatttca cggccttgag caggctcttg cacccgacta tgaccgcgcc atcgccattc gtcgtgagat tgtgaagaaa aagatatgtc catctccagc tgccacacat ccgctggaga gggttcccta caagaactac gactggagca gtgttgtggg acagagctgc gagaacatcc tcggctacgt tccggtaccg gtagggttgg ctggtccgct gctgttggat ggaaaggaag tggcactgcc gatggcgacg acggagggtg cgctggtcgc gagtgcgcac cgcggtgctc gcgccatcaa cctgagcggc ggatgccgga cagcggtgct gaaggaaggc atgacgcgcg cccctgtagt ggaggtgaac tccttcgacg aggccatcac cgtgatcaag ttctgtgagg agcggttcga cgttctaagg gaggccttcg agtcgacgac ccgcttcggg aagttgctga gcatcaagtg tgccatggcg ggtcgccagg tgcatctgcg cttctctgcc tttaccggag atgcgatggg catgaatatg atcacgaagg gctgcgacaa ggcgctgcag gtgctgcagc agcacattcc ctctgtgcga gtgctgacct tgtcaggcaa cttctgcact gacaagaaac cgtccgcact gaactgggtg gagggacgcg ggaagtcggt cgtagcggag gccgtcatca agcgtgatgt ggtcgagagt gttctcaagt gcacggtgga cagtgttgtg tcgctgaatg taactaagaa cctgcgcgga tctgcgcttg ccggttccat cggcggcttc aacgcacacg ctgccaacat cgtggccgcg ctctacatcg ccactggcca ggaccccgca caggtagtgg agtccgcaac ctgcatgacg acggttgaca aggctggtga ggatctcgtg atcagcctca tgatgccgag cattgaggtc ggcgccgtag gcggtggtac aggtctgtcg tcgcagcgcg ccatgctgga gctgatgggg tgcgcgggct ctaacaagga ggacccaggt gcccactccc gccagatcgc gcgtgtcgtt gctggcgcag tcatttgcgg tgagctgtcc ctcttgtccg gccttgcagc tggccactta ctaagcgccc acatgaagct gaaccgcaag cctccgactc cgtaa
Details for LemaA.18809.a.B2.GE41028
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS MSDTEIMKQV ENKKIAFHGL EQALAPDYDR AIAIRREIVK KKICPSPAAT HPLERVPYKN YDWSSVVGQS CENILGYVPV PVGLAGPLLL DGKEVALPMA TTEGALVASA HRGARAINLS GGCRTAVLKE GMTRAPVVEV NSFDEAITVI KFCEERFDVL REAFESTTRF GKLLSIKCAM AGRQVHLRFS AFTGDAMGMN MITKGCDKAL QVLQQHIPSV RVLTLSGNFC TDKKPSALNW VEGRGKSVVA EAVIKRDVVE SVLKCTVDSV VSLNVTKNLR GSALAGSIGG FNAHAANIVA ALYIATGQDP AQVVESATCM TTVDKAGEDL VISLMMPSIE VGAVGGGTGL SSQRAMLELM GCAGSNKEDP G
Validated NT Sequence
cacctgggtc ctccttgtta gagcccgcgc accccatcag ctccagcatg gcgcgctgcg acgacagacc tgtaccaccg cctacggcgc cgacctcaat gctcggcatc atgaggctga tcacgagatc ctcaccagcc ttgtcaaccg tcgtcatgca ggttgcggac tccactacct gtgcggggtc ctggccagtg gcgatgtaga gcgcggccac gatgttggca gcgtgtgcgt tgaagccgcc gatggaaccg gcaagcgcag atccgcgcag gttcttagtt acattcagcg acacaacact gtccaccgtg cacttgagaa cactctcgac cacatcacgc ttgatgacgg cctccgctac gaccgacttc ccgcgtccct ccacccagtt cagtgcggac ggtttcttgt cagtgcagaa gttgcctgac aaggtcagca ctcgcacaga gggaatgtgc tgctgcagca cctgcagcgc cttgtcgcag cccttcgtga tcatattcat gcccatcgca tctccggtaa aggcagagaa gcgcagatgc acctggcgac ccgccatggc acacttgatg ctcagcaact tcccgaagcg ggtcgtcgac tcgaaggcct cccttagaac gtcgaaccgc tcctcacaga acttgatcac ggtgatggcc tcgtcgaagg agttcacctc cactacaggg gcgcgcgtca tgccttcctt cagcaccgct gtccggcatc cgccgctcag gttgatggcg cgagcaccgc ggtgcgcact cgcgaccagc gcaccctccg tcgtcgccat cggcagtgcc acttcctttc catccaacag cagcggacca gccaacccta ccggtaccgg aacgtagccg aggatgttct cgcagctctg tcccacaaca ctgctccagt cgtagttctt gtagggaacc ctctccagcg gatgtgtggc agctggagat ggacatatct ttttcttcac aatctcacga cgaatggcga tggcgcggtc atagtcgggt gcaagagcct gctcaaggcc gtgaaatgca atcttcttat tctccacctg cttcatgatc tctgtatcgg acatggacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHSM SDTEIMKQVE NKKIAFHGLE QALAPDYDRA IAIRREIVKK KICPSPAATH PLERVPYKNY DWSSVVGQSC ENILGYVPVP VGLAGPLLLD GKEVALPMAT TEGALVASAH RGARAINLSG GCRTAVLKEG MTRAPVVEVN SFDEAITVIK FCEERFDVLR EAFESTTRFG KLLSIKCAMA GRQVHLRFSA FTGDAMGMNM ITKGCDKALQ VLQQHIPSVR VLTLSGNFCT DKKPSALNWV EGRGKSVVAE AVIKRDVVES VLKCTVDSVV SLNVTKNLRG SALAGSIGGF NAHAANIVAA LYIATGQDPA QVVESATCMT TVDKAGEDLV ISLMMPSIEV GAVGGGTGLS SQRAMLELMG CAGSNKEDPG AHSRQIAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccatgt ccgatacaga gatcatgaag caggtggaga ataagaagat tgcatttcac ggccttgagc aggctcttgc acccgactat gaccgcgcca tcgccattcg tcgtgagatt gtgaagaaaa agatatgtcc atctccagct gccacacatc cgctggagag ggttccctac aagaactacg actggagcag tgttgtggga cagagctgcg agaacatcct cggctacgtt ccggtaccgg tagggttggc tggtccgctg ctgttggatg gaaaggaagt ggcactgccg atggcgacga cggagggtgc gctggtcgcg agtgcgcacc gcggtgctcg cgccatcaac ctgagcggcg gatgccggac agcggtgctg aaggaaggca tgacgcgcgc ccctgtagtg gaggtgaact ccttcgacga ggccatcacc gtgatcaagt tctgtgagga gcggttcgac gttctaaggg aggccttcga gtcgacgacc cgcttcggga agttgctgag catcaagtgt gccatggcgg gtcgccaggt gcatctgcgc ttctctgcct ttaccggaga tgcgatgggc atgaatatga tcacgaaggg ctgcgacaag gcgctgcagg tgctgcagca gcacattccc tctgtgcgag tgctgacctt gtcaggcaac ttctgcactg acaagaaacc gtccgcactg aactgggtgg agggacgcgg gaagtcggtc gtagcggagg ccgtcatcaa gcgtgatgtg gtcgagagtg ttctcaagtg cacggtggac agtgttgtgt cgctgaatgt aactaagaac ctgcgcggat ctgcgcttgc cggttccatc ggcggcttca acgcacacgc tgccaacatc gtggccgcgc tctacatcgc cactggccag gaccccgcac aggtagtgga gtccgcaacc tgcatgacga cggttgacaa ggctggtgag gatctcgtga tcagcctcat gatgccgagc attgaggtcg gcgccgtagg cggtggtaca ggtctgtcgt cgcagcgcgc catgctggag ctgatggggt gcgcgggctc taacaaggag gacccaggtg cccactcccg ccagatcgcg cgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat