SSGCID: Target Page

LemaA.18726.a

Uncharacterized protein

CENTER ID:	LemaA.18726.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.18726.a.B1.GE37565	full length		1	341

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF.35.3900
RefSeq:	XP_003722784.1
UniProt:	E9AFL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQQKNLVRAM QKERYKMEKG FVAYTKDLIE FFRSTRASKN EKGSQTSEPA KQENTSRALE QFYESEELIK RMKWKRVTWD EEADYTSFSK RILDNDSMFK RTTAFSFLSI HVVKDPCNTD LVTIPLAQKT IATGTLTNWL LQEREPKTVD LYPVRGASER RSAFHVDWTS GAVKILDTAS AQEILTFLST VAPRLQAVQL MMEAQQTRQT ADIEKVRVRV GATLKFNSYE TSFWDDPKRK TSPDFVNPDE LEQFLSGMLK SAFLYRWFLK GQEIRVIPPG RPYYLDMEKK EVQIPANFAE YNWLKAHSHF EGVERIADSF RRFWWFWFCF GMILVGDVEL V

NT Sequence

atgcagcaga agaacctcgt gcgcgcgatg caaaaagagc gctacaagat ggaaaaggga tttgttgcat acacgaaaga tttaattgag ttttttcgct cgacaagagc ctcgaaaaat gagaaagggt ctcagacctc tgagccagca aagcaggaga acacctcccg cgcattagag cagttttacg aatcagagga gctgataaag cggatgaagt ggaaacgtgt cacgtgggat gaagaggccg attacacgag cttttccaag cggatcttgg ataacgactc catgttcaag cgcaccacag cattctcttt cttgtctatt catgtcgtga aagacccttg caacaccgat ctcgtgacca tcccgctcgc gcagaaaacg attgcgactg gcactctcac gaactggctg ctacaggaac gggagccaaa aaccgtagac ttgtaccctg ttcgcggagc atcggagcgc cgtagtgctt ttcacgtaga ctggacctcg ggtgccgtca agattttaga tacggcttca gcgcaagaga tcttgacatt tttgtcgact gtagcgcctc gtctgcaagc cgtgcaactg atgatggagg cccagcaaac tcggcagaca gctgacattg aaaaggtgcg cgttcgagtg ggggccacgt tgaaattcaa ctcgtatgag acgagttttt gggatgaccc aaaacggaaa acgagccctg acttcgtcaa ccctgacgaa ttagagcagt tcctctccgg catgcttaaa tccgcgttcc tgtaccgctg gtttttgaag ggacaggaaa tcagagtgat accaccaggt cgtccttact acctggacat ggaaaagaag gaggttcaga ttcccgccaa ttttgcagag tacaactggc tgaaggccca ctcgcatttt gagggagttg aaaggattgc tgatagtttt cgtaggtttt ggtggttttg gttttgtttc ggtatgatcc ttgttggaga tgtggagttg gtgtag

Details for LemaA.18726.a.B1.GE37565

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	105

Validated AA Sequence

MAHHHHHHMQ QKNLVRAMQK ERYKMEKGFV AYTKDLIEFF RSTRASKNEK GSQTSEPAKQ ENTSRALEQF YESEELIKRM KWKRVTWDEE ADYTSFSKRI LDNDSMFKRT TAFSFLSIHV VKDPCNTDLV TIPLAQKTIA TGTLTNWLLQ EREPKTVDLY PVRGASERRS AFHVDWTSGA VKILDTASAQ EILTFLSTVA PRLQAVQLMM EAQQTRQTAD IEKVRVRVGA TLKFNSYETS FWDDPKRKTS PDFVNPDELE QFLSGMLKSA FLYRWFLKGQ EIRVIPPGRP YYLDMEKKEV QIPANFAEYN WLXAHSHFEG VEXLLIVFVV LVXLVCFGMI LVXCXXVXSR XXXXPPXXXX XXXGAXX

Validated NT Sequence

cnnccnccnn gnaanatttn ngtttacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgcagcaga agaacctcgt gcgcgcgatg caaaaagagc gctacaagat ggaaaaggga tttgttgcat acacgaaaga tttaattgag ttttttcgct cgacaagagc ctcgaaaaat gagaaagggt ctcagacctc tgagccagca aagcaggaga acacctcccg cgcattagag cagttttacg aatcagagga gctgataaag cggatgaagt ggaaacgtgt cacgtgggat gaagaggccg attacacgag cttttccaag cggatcttgg ataacgactc catgttcaag cgcaccacag cattctcttt cttgtctatt catgtcgtga aagacccttg caacaccgat ctcgtgacca tcccgctcgc gcagaaaacg attgcgactg gcactctcac gaactggctg ctacaggaac gggagccaaa aaccgtagac ttgtaccctg ttcgcggagc atcggagcgc cgtagtgctt ttcacgtaga ctggacctcg ggtgccgtca agattttaga tacggcttca gcgcaagaga tcttgacatt tttgtcgact gtagcgcctc gtctgcaagc cgtgcaactg atgatggagg cccagcaaac tcggcagaca gctgacattg aaaaggtgcg cgttcgagtg ggggccacgt tgaaattcaa ctcgtatgag acgagttttt gggatgaccc aaaacggaaa acgagccctg acttcgtcaa ccctgacgaa ttagagcagt tcctctccgg catgcttaaa tccgcgttcc tgtaccgctg gtttttgaag ggacaggaaa tcagagtgat accaccaggt cgtccttact acctggacat ggaaaagaag gaggttcaga ttcccgccaa ttttgcagag tacaactggc tgaangccca ctcgcatttt gagggagttg aaannttgct gatagttttc gtagttttgg tggntttggt ttgtttcggt atgatccttg tnnnatgtgn nnnngtantg agtagannnn nnnnnnnccc ccccnnnnnn nnnnnnnnan nannnggngc nttnngaca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc agaagaacct cgtgcgcgcg atgcaaaaag agcgctacaa gatggaaaag ggatttgttg catacacgaa agatttaatt gagttttttc gctcgacaag agcctcgaaa aatgagaaag ggtctcagac ctctgagcca gcaaagcagg agaacacctc ccgcgcatta gagcagtttt acgaatcaga ggagctgata aagcggatga agtggaaacg tgtcacgtgg gatgaagagg ccgattacac gagcttttcc aagcggatct tggataacga ctccatgttc aagcgcacca cagcattctc tttcttgtct attcatgtcg tgaaagaccc ttgcaacacc gatctcgtga ccatcccgct cgcgcagaaa acgattgcga ctggcactct cacgaactgg ctgctacagg aacgggagcc aaaaaccgta gacttgtacc ctgttcgcgg agcatcggag cgccgtagtg cttttcacgt agactggacc tcgggtgccg tcaagatttt agatacggct tcagcgcaag agatcttgac atttttgtcg actgtagcgc ctcgtctgca agccgtgcaa ctgatgatgg aggcccagca aactcggcag acagctgaca ttgaaaaggt gcgcgttcga gtgggggcca cgttgaaatt caactcgtat gagacgagtt tttgggatga cccaaaacgg aaaacgagcc ctgacttcgt caaccctgac gaattagagc agttcctctc cggcatgctt aaatccgcgt tcctgtaccg ctggtttttg aagggacagg aaatcagagt gataccacca ggtcgtcctt actacctgga catggaaaag aaggaggttc agattcccgc caattttgca gagtacaact ggctgaaggc ccactcgcat tttgagggag ttgaaaggat tgctgatagt tttcgtaggt tttggtggtt ttggttttgt ttcggtatga tccttgttgg agatgtggag ttggtgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t