LemaA.18719.a

Uncharacterized protein

CENTER ID: LemaA.18719.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18719.a.B1.GE37564 full length 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LmjF.07.1110
RefSeq: XP_001681023.1
UniProt: Q4QIH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLKHVASFG AVGVLSVVGM LGGRRWHRVE LRRAELNEEY TKLMNEMRTF NEKRLTRDER LAAKEAEAKV TAETVDILWS DRLARYAQVN KDLHAYLAAL PEAIGVLKGL SNHYRYMSEE MPKFTGFDIA CSKMHNLALM LEHGKAVGIE RVAATVQAMF VAEPLVQAVC TSILAAPAPP HPSSIAAASA AFTFCMEELD RAVGTVAMRY AAALEEPPNA TPGILSDSVR KLVSMMRTDT LCKGQRQLAE RRRDLERTLR RAQRQLHTEE DIRAALDYTR ELDQHLQAAA PRRTDLLLSS PSRKDNFLAA VRSDSEVKKA IQQIDLWRDS ATTFLVHRQA EDALQSYYFL LAETLTAVNE LK
NT Sequence
atgtcactca agcatgtggc gtcttttggc gctgtcggcg tcttatcggt ggtcggcatg cttggaggac ggcgatggca ccgcgtcgag ctgcgccgcg ccgagctaaa tgaggagtac acgaagctca tgaatgaaat gcgtaccttc aacgagaagc gtctcacccg cgatgagcgc ctggccgcca aggaggcgga ggcgaaggtg acggcggaga cggtggacat attatggagc gatcggctgg cgcgttatgc gcaggtgaac aaggacctgc atgcctacct ggcggctttg cctgaggcga tcggcgtact gaagggtctg tcgaatcact atcgctacat gtcggaggag atgcccaagt tcacggggtt cgacatcgcg tgctccaaga tgcacaacct ggcattgatg ctggagcacg gcaaggcggt aggcattgag cgtgtggcgg cgacagtgca ggcaatgttt gtcgcggagc cacttgtgca ggcggtgtgc acgagcatct tggcagcccc ggctccgccg catcccagtt cgatcgctgc ggccagcgcc gccttcacgt tctgcatgga agagctcgac cgcgccgtgg ggacggttgc tatgcggtat gcggcagcac tggaagagcc cccaaatgcc acgcctggca tcttgtcaga tagtgtccgg aagcttgtca gtatgatgcg cacagacacg ctttgcaagg ggcagcgcca gcttgcggag cgcagaaggg acttggaacg gacgcttcgc cgtgcccagc gccagctgca caccgaggag gacattcggg cagcgctgga ttacacgagg gagctggatc agcaccttca ggcggctgca ccacgaagaa ccgacttgtt gctttcatcg ccttccagga aggataactt tctggccgcc gtgcggtcgg atagtgaggt gaaaaaggcc atccagcaga tcgacctgtg gcgtgactcg gcaaccactt tcctggtgca ccgccaggcc gaggacgcgc tgcagagcta ctacttcctc ctggccgaaa cgcttacagc agtgaatgag ttgaagtag
Details for LemaA.18719.a.B1.GE37564
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMS LKHVASFGAV GVLSVVGMLG GRRWHRVELR RAELNEEYTK LMNEMRTFNE KRLTRDERLA AKEAEAKVTA ETVDILWSDR LARYAQVNKD LHAYLAALPE AIGVLKGLSN HYRYMSEEMP KFTGFDIACS KMHNLALMLE HGKAVGIERV AATVQAMFVA EPLVQAVCTS ILAAPAPPHP SSIAAASAAF TFCMEELDRA VGTVAMRYAA ALEEPPNATP GILSDSVRKL VSMMRTDTLC KGQRQLAERR KGLGTDASPC PAPAAHRGGH SGSAGLHEGA GSAPSGGCTT KNRLVAFIAF XXDNFLAAVR SDSEXKKAIQ QIDLCVTRHH FXGARXXTXC RXTTLXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
cnnnnnanna tnnttttntt taactttaag aaggagatat accatggctc accaccacca ccaccatatg tcactcaagc atgtggcgtc ttttggcgct gtcggcgtct tatcggtggt cggcatgctt ggaggacggc gatggcaccg cgtcgagctg cgccgcgccg agctaaatga ggagtacacg aagctcatga atgaaatgcg taccttcaac gagaagcgtc tcacccgcga tgagcgcctg gccgccaagg aggcggaggc gaaggtgacg gcggagacgg tggacatatt atggagcgat cggctggcgc gttatgcgca ggtgaacaag gacctgcatg cctacctggc ggctttgcct gaggcgatcg gcgtactgaa gggtctgtcg aatcactatc gctacatgtc ggaggagatg cccaagttca cggggttcga catcgcgtgc tccaagatgc acaacctggc attgatgctg gagcacggca aggcggtagg cattgagcgt gtggcggcga cagtgcaggc aatgtttgtc gcggagccac ttgtgcaggc ggtgtgcacg agcatcttgg cagccccggc tccgccgcat cccagttcga tcgctgcggc cagcgccgcc ttcacgttct gcatggaaga gctcgaccgc gccgtgggga cggttgctat gcggtatgcg gcagcactgg aagagccccc aaatgccacg cctggcatct tgtcagatag tgtccggaag cttgtcagta tgatgcgcac agacacgctt tgcaaggggc agcgccagct tgcggagcgc agaaagggac ttggaacgga cgcttcgccg tgcccagcgc cagctgcaca ccgaggagga cattcgggca gcgctggatt acacgaggga gctggatcag caccttcagg cggctgcacc acgaagaacc gacttgttgc tttcatcgcc ttccnnnagg ataactttct ggccgccgtg cggtcggata gtgagnngaa aaaggccatc cagcagatcg acctgtgcgt gactcggcac cactttnctg gtgcacgcnn nnagacgcnc tgcagancta ctactctctg nnaangcttn nncnnnannn tgantangnn nannnacnnn nnnnnnnnca nnaananann nnnttnnnnn cntnng
Expected Protein Sequence
MAHHHHHHMS LKHVASFGAV GVLSVVGMLG GRRWHRVELR RAELNEEYTK LMNEMRTFNE KRLTRDERLA AKEAEAKVTA ETVDILWSDR LARYAQVNKD LHAYLAALPE AIGVLKGLSN HYRYMSEEMP KFTGFDIACS KMHNLALMLE HGKAVGIERV AATVQAMFVA EPLVQAVCTS ILAAPAPPHP SSIAAASAAF TFCMEELDRA VGTVAMRYAA ALEEPPNATP GILSDSVRKL VSMMRTDTLC KGQRQLAERR RDLERTLRRA QRQLHTEEDI RAALDYTREL DQHLQAAAPR RTDLLLSSPS RKDNFLAAVR SDSEVKKAIQ QIDLWRDSAT TFLVHRQAED ALQSYYFLLA ETLTAVNELK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac tcaagcatgt ggcgtctttt ggcgctgtcg gcgtcttatc ggtggtcggc atgcttggag gacggcgatg gcaccgcgtc gagctgcgcc gcgccgagct aaatgaggag tacacgaagc tcatgaatga aatgcgtacc ttcaacgaga agcgtctcac ccgcgatgag cgcctggccg ccaaggaggc ggaggcgaag gtgacggcgg agacggtgga catattatgg agcgatcggc tggcgcgtta tgcgcaggtg aacaaggacc tgcatgccta cctggcggct ttgcctgagg cgatcggcgt actgaagggt ctgtcgaatc actatcgcta catgtcggag gagatgccca agttcacggg gttcgacatc gcgtgctcca agatgcacaa cctggcattg atgctggagc acggcaaggc ggtaggcatt gagcgtgtgg cggcgacagt gcaggcaatg tttgtcgcgg agccacttgt gcaggcggtg tgcacgagca tcttggcagc cccggctccg ccgcatccca gttcgatcgc tgcggccagc gccgccttca cgttctgcat ggaagagctc gaccgcgccg tggggacggt tgctatgcgg tatgcggcag cactggaaga gcccccaaat gccacgcctg gcatcttgtc agatagtgtc cggaagcttg tcagtatgat gcgcacagac acgctttgca aggggcagcg ccagcttgcg gagcgcagaa gggacttgga acggacgctt cgccgtgccc agcgccagct gcacaccgag gaggacattc gggcagcgct ggattacacg agggagctgg atcagcacct tcaggcggct gcaccacgaa gaaccgactt gttgctttca tcgccttcca ggaaggataa ctttctggcc gccgtgcggt cggatagtga ggtgaaaaag gccatccagc agatcgacct gtggcgtgac tcggcaacca ctttcctggt gcaccgccag gccgaggacg cgctgcagag ctactacttc ctcctggccg aaacgcttac agcagtgaat gagttgaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat