LemaA.18709.a

Aurora kinase 2, putative (AUK2)

CENTER ID: LemaA.18709.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18709.a.B2.GE42803 PHoranyi kinase domain truncation 260 589
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.29.1330
UniProt: E9ADZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLPCPSSSS PLLGSITSGV RSPSRGPVQQ QQLLHQRHAS MRLRLHKDGS ICACPEKSGG GVSQACAPPL PATSAFARGK EEPSAKRHHA EPSSCPGPGG DSSSVDRSAA ATVVTIAAPL LSIPNPSSRA APSPFRFLCT SVGGCPVVGA KDARVHLPDS SARCDVSGSV APRDLSTTSA HSKAHSTAAV GRASPSSGVV SPPLSATYPS LVSASHGARV VCSHPQSASA STVPGATIDT SAPSPVPSPP HRIHQHELRP ADFRREEVLG EGSYSIVTLA THRASGLSFA LKEIDRSRLR WRPLEAQLRW EINLQRTLRH PHIVRLYSYF ITPDCISLVL EYCSGGTLLS RLRAAPQYRL SERQASRYTR HVAKALVHLH GLGVAHRDLK LENVLIDADG IAKLADFGWS RSVVHPLPSS KSAAAAQTAG DRNDAPCSSA EHNTSNEDPT EGVAEGRRTV CGTLDYLSPE MVSGQAHSAK TDVWSLGVML AEMLTGTPPF YTESTQETLY AIQRLPPNLT GLHPKGHGPG LTGSGASTSA SVCLILDEPV ALSAGALSLI HAMLQKDPSA RPTMPEVLGH PWLQKKKLAR KS
NT Sequence
atgtcattgc cgtgcccctc ctcatcctcg cctctgctag gcagcatcac cagcggtgtt cgctcgccgt caaggggacc agtgcagcag cagcagctgc tgcaccagcg acatgcctcg atgcgactgc gtctccacaa ggacggcagc atttgcgcat gcccagagaa gtctggtgga ggtgtcagcc aagcatgcgc tccgccgctg ccggctactt cggcctttgc tcgagggaag gaggaaccga gcgcaaagcg tcatcatgca gagccgtcat cttgcccagg accaggcggc gacagcagtt ctgtcgaccg gagtgcggcg gcgacagtcg tcaccattgc cgctcctctg ctttctatac caaacccctc ttcgcgggct gcgccttcgc cttttcgttt tctgtgcaca tctgtcggtg ggtgcccggt ggtcggggcg aaggatgcac gcgttcacct gccagactcg tcagcacgtt gtgacgtgag cggcagtgtc gctccgaggg acctttcgac cacatctgcg cactcaaagg cgcattccac ggcggccgtt gggcgggcct cgccctcgtc gggcgtcgtg tcaccaccgc tctctgcgac ttacccctcc ctcgtatctg cgtctcacgg cgcacgcgtc gtttgctctc atccgcagtc cgcatcggcg tccaccgtgc ctggcgctac cattgacaca tcagcaccgt cgccagtgcc atcgccgccg caccgcatcc atcaacacga gcttcgcccc gcggatttcc gtcgagagga ggtcctcggt gaagggagct attcgatcgt cacccttgcc acgcaccgtg cgtcgggctt gtctttcgcg ctgaaggaga tcgaccgcag ccgcctgcga tggcgaccgc tggaggcgca gctgcgctgg gagatcaatt tgcagcggac gctgcgccac ccgcacatcg tgcgactcta cagctacttc attacgccag actgcatcag tcttgtgttg gagtactgca gcggcgggac gctgctgagt cggttgagag ctgcaccaca gtaccgcttg tcggagcggc aggcatcgcg ctacactcgc cacgtggcga aggcgctggt acatctgcac gggctcggag tggcgcaccg agacctgaag ctggaaaatg tgctcatcga tgcagacggc atcgccaagc tggcagactt tggctggtcg cgctctgtcg tgcatccgtt gccatccagt aagtcagcgg ctgctgctca gactgcaggc gacaggaacg acgcaccatg tagcagcgcg gagcacaaca cgagcaacga ggacccgacg gaaggggtgg cagagggtcg ccgcaccgtg tgtggcacgc tggactacct ctcacctgag atggtctccg gccaagcaca ctcggccaag acagacgtct ggtcgcttgg tgtcatgctg gccgagatgc tgaccggcac gccgcccttc tacaccgagt cgactcagga gacgctttac gccatccagc ggctgccgcc caacctcacc ggcttacacc ctaaaggcca cggaccaggg ctcactggta gcggtgcctc cacctcggcc agcgtgtgtc tgatcctcga cgaacctgtg gccctatctg caggcgcgct ttctctcatt cacgctatgc tccagaagga cccgtcggcg cgaccgacta tgccggaggt gctgggccac ccctggctgc aaaaaaaaaa actcgctagg aagtcatga
Details for LemaA.18709.a.B2.GE42803
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 67
PERCENT COVERAGE: 83
Validated AA Sequence
MAHHHHHHMP ADFXXXEVLG XXXYSIVTLA XXRAXXLSFA LKXXDRXRLR WXPLEAQLRW EINLQRTXRH PHXVRLYXXX ITPDCIXLVL EYXXGGXXLX XXXXXXXXRL SXXQASRYTR XXAKALVHLH GLGVAHRDLK LXNVLIDAXG IAKXADFGWS RSVVHPLPSS KSAAAAQTAG DRNDAPCSSA EHNTSNEDPT EGVAEGXRTX CGTLDYLSPE MVXXPSTLXQ DRRLXXWCHA XXDADRHAAX XHRXDSGDAL RHPAAAAQPH RLTXXRPRXR AHW
Validated NT Sequence
gtggcccnnn ncctccggca nnnnnngtcg cgcnnnnngg tccttctgga nnnnngcgtn aangnnnnaa agcgcgcctg nnnntnnngc cacaggttcg tcgaggatca gacacannct ggccgaggtg gaggcaccgc taccagtgag ccctnnnccg tggcctttnn ngngtaagcc ggtgaggttg ggcggcagcc gctggatggc gtaaagcgtc tcctgagtcn nctcggtgnn nnnnggcggc gtgccggtca gcatctcnnn nngcatgaca ccaannnnnc agacgtctgt cttgnncgag tgtgcttggc nggannacca tctcaggtga gaggtagtcn agcgtgccac acnnngtgcg nnnaccctct gccacccctt ccgtcgggtc ctcgttgctc gtgttgtgct ccgcgctgct acatggtgcg tcgttcctgt cgcctgcagt ctgagcagca gccgctgact tactggatgg caacggatgc acgacagagc gcgaccagcc aaagtctgcn ngcttggcga tgccgnnngc atcgatgagc acattnnnna gcttcaggtc tcggtgcgcc actccgagcc cgtgcagatg taccagcgcc ttcgccnnnn ggcgagtgta gcgcgatgcc tgcnnnnncg acaagcggtn nnntnnnnna nnnnncannn nnntcagcan nnncccgccg nnncagtact ccaacacaag nctgatgcag tctggcgtaa tnaagtnnnn gtagagtcgc acgntgtgcg ggtggcgnnn ngtccgctgc aaattgatct cccagcgcag ctgcgcctcc agcggnnncc atcgcaggcg nnngcggtcg anntccttca gcgcgaaaga caagcnnnnc gcacggtnnn tggcaagggt gacgatcgaa tagntnnnnt caccgaggac ctcctnnnnn nggaaatccg cgggcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHPA DFRREEVLGE GSYSIVTLAT HRASGLSFAL KEIDRSRLRW RPLEAQLRWE INLQRTLRHP HIVRLYSYFI TPDCISLVLE YCSGGTLLSR LRAAPQYRLS ERQASRYTRH VAKALVHLHG LGVAHRDLKL ENVLIDADGI AKLADFGWSR SVVHPLPSSK SAAAAQTAGD RNDAPCSSAE HNTSNEDPTE GVAEGRRTVC GTLDYLSPEM VSGQAHSAKT DVWSLGVMLA EMLTGTPPFY TESTQETLYA IQRLPPNLTG LHPKGHGPGL TGSGASTSAS VCLILDEPVA LSAGALSLIH AMLQKDPSAR PTMPEVLGHP WLQKKKLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcccgcgg atttccgtcg agaggaggtc ctcggtgaag ggagctattc gatcgtcacc cttgccacgc accgtgcgtc gggcttgtct ttcgcgctga aggagatcga ccgcagccgc ctgcgatggc gaccgctgga ggcgcagctg cgctgggaga tcaatttgca gcggacgctg cgccacccgc acatcgtgcg actctacagc tacttcatta cgccagactg catcagtctt gtgttggagt actgcagcgg cgggacgctg ctgagtcggt tgagagctgc accacagtac cgcttgtcgg agcggcaggc atcgcgctac actcgccacg tggcgaaggc gctggtacat ctgcacgggc tcggagtggc gcaccgagac ctgaagctgg aaaatgtgct catcgatgca gacggcatcg ccaagctggc agactttggc tggtcgcgct ctgtcgtgca tccgttgcca tccagtaagt cagcggctgc tgctcagact gcaggcgaca ggaacgacgc accatgtagc agcgcggagc acaacacgag caacgaggac ccgacggaag gggtggcaga gggtcgccgc accgtgtgtg gcacgctgga ctacctctca cctgagatgg tctccggcca agcacactcg gccaagacag acgtctggtc gcttggtgtc atgctggccg agatgctgac cggcacgccg cccttctaca ccgagtcgac tcaggagacg ctttacgcca tccagcggct gccgcccaac ctcaccggct tacaccctaa aggccacgga ccagggctca ctggtagcgg tgcctccacc tcggccagcg tgtgtctgat cctcgacgaa cctgtggccc tatctgcagg cgcgctttct ctcattcacg ctatgctcca gaaggacccg tcggcgcgac cgactatgcc ggaggtgctg ggccacccct ggctgcaaaa aaaaaaactc gcttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat