LemaA.18681.a

bifunctional aminoacyl-tRNA synthetase, putative

CENTER ID: LemaA.18681.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18681.a.B1.GE42690 full length 1 731
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.18.1220
UniProt: Q4QDS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKDCRGEP ELLALLKELN IELPTISHGE MHTVEEANRE LVQFGTPCVG TKNMFLKSKK GELVLLTAVH TTKTDMHAIE KAAGTRNLRF APAEILSENL AVVQGCVTPF ALINNIEKHN VTVLLDKNLK DSPIPFVLHP CRNDKSSLVV FEQLERFLEK VGYAYKLVDF GAASADAAVT GGAAAPAPKP KKAPADAAAA KRGGGGGGAA APASAQSGET KLGIAAKREE NFSAWYIDVI TKAEMIEYYD VSGCYIIRPW AYYVWKCVQR FLGGKIEKLG VEDCYFPMFV SRSCLEREKD HIEGFAPEVA WVTRAGDTEL EQPVAVRPTS ETVMYPYYAK WIRSHRDLPV RLNMWNNVIR WEFSHPTPFI RTREFLWQEG HCAWAKAEEC AKEVLEILEC YASVYEQLLA VPVVRGRKTE KEKFAGGDYT TTVETFIEAV GRGCQGATSH NLGQNFGKMF DIRFQDPENN EQTLIPWQNS WGLSTRVIGV MIMVHGDNRG MVMPPRVAST QVIIIPVGIT KDTTEEARQE LLASCWRLES ELCEGGVRAR CDLRDNYSPG WRFNHWEVKG VPLRVELGPR ELAERSLAVA VRHSGARHSV AWDAQTPAAV AALLEDVHAQ MYARAKETME THRVRVTEWT EFVPTLNRKC LILAPWCGAM ECENQVKKDS AEESKVAQAQ ETREDARAPS MGAKTLCIPF EQPEEPAEGH ECICKGCTKP ATTWVLFGRS Y
NT Sequence
atgtccgcga aggactgcag gggagagccg gagctgctcg cattgttgaa ggagctcaac attgagctgc ctactatctc gcatggggag atgcacacgg tagaagaggc gaatagggag ctggtccaat ttggcactcc ttgtgtgggc accaagaaca tgttcctcaa gagcaagaag ggcgaactgg tactgctcac agcggtgcac acgaccaaga cggacatgca tgcaatcgag aaggcggccg gaaccaggaa tctccggttt gccccggcag agatcctcag tgaaaatctc gccgtcgtac agggctgcgt cacaccgttt gccctcatca acaacatcga gaagcacaac gtcacggtgc tcttggataa gaacctgaaa gacagcccca tccccttcgt gctacacccc tgccgcaacg ataagagctc cctcgttgtc tttgagcagc tggagcgctt cctggagaag gtcgggtatg cgtacaagct ggtggacttt ggtgcggctt cggcggacgc tgcggtgacg ggcggcgctg ctgctcctgc tccgaagccg aagaaggcgc ccgcggacgc cgctgcggcg aagcgtggtg gtggtggtgg tggtgctgct gcgcctgcgt cggcacagtc tggcgagacg aagctgggca tcgcggcgaa gcgcgaggag aacttttctg cgtggtacat cgatgtgatc acgaaggcgg agatgatcga gtactacgac gtgtccgggt gctacattat ccgtccgtgg gcgtactacg tatggaagtg cgtgcagcgc ttccttggcg ggaagatcga gaaacttggc gtggaggact gctacttccc gatgttcgtg tcgcgcagct gcctggagcg ggagaaggac cacatcgagg gctttgcgcc ggaggttgca tgggtgacgc gcgccggcga cacggagctg gagcagccgg ttgccgtgcg gccgacgagc gagacggtga tgtacccgta ctacgcgaag tggatccggt cgcaccgcga cctgcccgtg cggctgaaca tgtggaacaa cgtgatccgg tgggagttct cgcacccgac gccgttcatt cggactcgcg agttcctgtg gcaggagggg cactgcgcgt gggcgaaggc ggaggagtgc gcgaaggagg tgcttgaaat cctggagtgc tatgcgtcgg tgtacgaaca gctactggcg gtgccggtgg tgcgcgggcg caagacggag aaggagaagt tcgctggcgg ggactacacg acgacggtgg agacgttcat cgaggctgtt gggcgcgggt gccagggcgc gacgagccac aacctggggc agaacttcgg caagatgttc gacatccgct tccaggaccc ggagaacaac gagcagacgc tgatcccgtg gcagaacagc tgggggctgt cgacgcgcgt gatcggcgtg atgatcatgg tgcacggcga caaccgcggc atggtgatgc cgccgcgcgt tgcgtcgacg caggtgatca tcatccctgt ggggatcacg aaggacacga cggaggaggc gcggcaggag ctgcttgcaa gctgctggcg gctggagagc gagctgtgcg agggcggcgt gcgcgcgagg tgcgacctgc gcgacaacta cagccctggg tggcggttca accactggga ggtgaagggc gtgccgctgc gcgtggagct tgggccgagg gagcttgcgg agcggtcgct tgcggttgct gtgcggcaca gcggcgcgag gcactcggtt gcgtgggacg cgcagacgcc ggcggcggta gccgcgctgc tggaggatgt gcatgcacag atgtacgcgc gcgcgaagga gacgatggag acgcatcgcg tgcgtgtgac ggagtggacg gagtttgtgc cgacgctgaa ccggaagtgc ttgatccttg cgccgtggtg cggcgcgatg gagtgcgaga accaggtaaa gaaggacagc gcggaggagt cgaaggttgc acaggcgcag gagacgcgcg aggatgcgcg tgcgccgagc atgggagcga agacgctgtg catcccgttt gagcagccgg aggagcccgc agagggtcac gagtgcatct gcaagggctg cacgaagccg gcgacgacgt gggtgctgtt tggccgcagc tactga
Details for LemaA.18681.a.B1.GE42690
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 83kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS AKDCRGEPEL LALLKELNIE LPTISHGEMH TVEEANRELV QFGTPCVGTK NMFLKSKKGE LVLLTAVHTT KTDMHAIEKA AGTRNLRFAP AEILSENLAV VQGCVTPFAL INNIEKHNVT VLLDKNLKDS PIPFVLHPCR NDKSSLVVFE QLERFLEKVG YAYKLVDFGA ASADAAVTGG AAAPAPKPKK APADAAAAKR GGGGGGAAAP ASAQSGETKL GIAAKREENF SAWYIDVITK AEMIEYYDVS GCYIIRPWAY YVWKCVQRFL GGKIEKLGVE DCYFPMFVSR SCLEREKDHI EGFAPEVAWV TRAGDTELEQ PVAVRPTSET VMYPYYAKWI RSHRDLPVRL NMWNNVIRWE FSHPTPFIRT REFLWQEGHC AWAKAEECAK EVLEILECYA SVYEQLLAVP VVRGRKTEKE KFAGGDYTTT VETFIEAVGR GCQGATSHNL GQNFGKMFDI RFQDPENNEQ TLIPWQNSWG LSTRVIGVMI MVHGDNRGMV MPPRVASTQV IIIPVGITKD TTEEARQELL ASCWRLESEL CEGGVRARCD LRDNYSPGWR FNHWEVKGVP LRVELGPREL AERSLAVAVR HSGARHSVAW DAQTPAAVAA LLEDVHAQMY ARAKETMETH RVRVTEWTEF VPTLNRKCLI LAPWCGAMEC ENQVKKDSAE ESKVAQAQET REDARAPSMG AKTLCIPFEQ PEEPAEGHEC ICKGCTKPAT TWVLFGRSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cgaaggactg caggggagag ccggagctgc tcgcattgtt gaaggagctc aacattgagc tgcctactat ctcgcatggg gagatgcaca cggtagaaga ggcgaatagg gagctggtcc aatttggcac tccttgtgtg ggcaccaaga acatgttcct caagagcaag aagggcgaac tggtactgct cacagcggtg cacacgacca agacggacat gcatgcaatc gagaaggcgg ccggaaccag gaatctccgg tttgccccgg cagagatcct cagtgaaaat ctcgccgtcg tacagggctg cgtcacaccg tttgccctca tcaacaacat cgagaagcac aacgtcacgg tgctcttgga taagaacctg aaagacagcc ccatcccctt cgtgctacac ccctgccgca acgataagag ctccctcgtt gtctttgagc agctggagcg cttcctggag aaggtcgggt atgcgtacaa gctggtggac tttggtgcgg cttcggcgga cgctgcggtg acgggcggcg ctgctgctcc tgctccgaag ccgaagaagg cgcccgcgga cgccgctgcg gcgaagcgtg gtggtggtgg tggtggtgct gctgcgcctg cgtcggcaca gtctggcgag acgaagctgg gcatcgcggc gaagcgcgag gagaactttt ctgcgtggta catcgatgtg atcacgaagg cggagatgat cgagtactac gacgtgtccg ggtgctacat tatccgtccg tgggcgtact acgtatggaa gtgcgtgcag cgcttccttg gcgggaagat cgagaaactt ggcgtggagg actgctactt cccgatgttc gtgtcgcgca gctgcctgga gcgggagaag gaccacatcg agggctttgc gccggaggtt gcatgggtga cgcgcgccgg cgacacggag ctggagcagc cggttgccgt gcggccgacg agcgagacgg tgatgtaccc gtactacgcg aagtggatcc ggtcgcaccg cgacctgccc gtgcggctga acatgtggaa caacgtgatc cggtgggagt tctcgcaccc gacgccgttc attcggactc gcgagttcct gtggcaggag gggcactgcg cgtgggcgaa ggcggaggag tgcgcgaagg aggtgcttga aatcctggag tgctatgcgt cggtgtacga acagctactg gcggtgccgg tggtgcgcgg gcgcaagacg gagaaggaga agttcgctgg cggggactac acgacgacgg tggagacgtt catcgaggct gttgggcgcg ggtgccaggg cgcgacgagc cacaacctgg ggcagaactt cggcaagatg ttcgacatcc gcttccagga cccggagaac aacgagcaga cgctgatccc gtggcagaac agctgggggc tgtcgacgcg cgtgatcggc gtgatgatca tggtgcacgg cgacaaccgc ggcatggtga tgccgccgcg cgttgcgtcg acgcaggtga tcatcatccc tgtggggatc acgaaggaca cgacggagga ggcgcggcag gagctgcttg caagctgctg gcggctggag agcgagctgt gcgagggcgg cgtgcgcgcg aggtgcgacc tgcgcgacaa ctacagccct gggtggcggt tcaaccactg ggaggtgaag ggcgtgccgc tgcgcgtgga gcttgggccg agggagcttg cggagcggtc gcttgcggtt gctgtgcggc acagcggcgc gaggcactcg gttgcgtggg acgcgcagac gccggcggcg gtagccgcgc tgctggagga tgtgcatgca cagatgtacg cgcgcgcgaa ggagacgatg gagacgcatc gcgtgcgtgt gacggagtgg acggagtttg tgccgacgct gaaccggaag tgcttgatcc ttgcgccgtg gtgcggcgcg atggagtgcg agaaccaggt aaagaaggac agcgcggagg agtcgaaggt tgcacaggcg caggagacgc gcgaggatgc gcgtgcgccg agcatgggag cgaagacgct gtgcatcccg tttgagcagc cggaggagcc cgcagagggt cacgagtgca tctgcaaggg ctgcacgaag ccggcgacga cgtgggtgct gtttggccgc agctactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t