LemaA.18331.a

Putative fumarate hydratase (EC 4.2.1.2) (class I, cytosolic)

CENTER ID: LemaA.18331.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18331.a.B1.GE42079 full length 1 568
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.29.1960
UniProt: E9AE57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLCDQCEIG CRRVGIKDIE DASAVNADFH FSAIFQPTDP HHHQTEFAKV EGSEKYVEEV EVFGRQALKV NPEALTILAH RAFSDVHHFF RKDHLEGWRR AIEDPEASDN DRYVATTLLK NACIAAGRVL PSCQDTGTAI VLGKRGELCW TGGEDEKYLS KGIWNAYRYH NLRYSQTAAL DMFKECNTGD NLPAQLDLLA VPGSDYEFLF IAKGGGSANK AYLYQETKAL LNPKSLRAFI EEKLKTLGTA ACPPYHIALV IGGTSAEMTM KTVKLASCRY YDSLPTTGDK YGRAFRDPEW EKIVMEVAQK SGIGAQFGGK YFAHQARVIR LPRHGASCPV GLAVSCSADR QILAHINKSG IYIEQLEQNP AQYLPDIPEV HLSTTSVKVD LKRPIDKVRQ QLSQYPVGTR VMLNGTLIVA RDIAHAKIKE MMDNGEPLPE YMKTSPIYYA GPAKTPEGYA SGSFGPTTAG RMDSYVDLFQ SHGGSYITLA KGNRSKQVTD ACKKHGGFYL GSIGGPAAIL AKDSIKQVTC LAFPELGMEA VWKIEVEDFP AFIVVDDKGN DMYSKTLA
NT Sequence
atgtctctgt gcgaccagtg cgagatcggg tgccgccgtg tgggcatcaa ggacatcgag gacgcgtccg ctgtcaacgc agacttccac ttctccgcca tcttccagcc caccgacccg caccatcacc aaaccgagtt cgccaaggtc gaggggagcg agaagtacgt ggaggaggtg gaggtgttcg ggcgccaggc gctgaaggtg aaccccgagg ccctcactat tctcgcccac cgcgccttct cggatgtgca ccacttcttc cgaaaggacc acctggaggg ttggcgccgc gccatcgagg accccgaggc ctccgacaac gaccgctacg tcgccacgac gctgctgaag aacgcctgca tcgcggcagg tcgggtgctg ccgtcgtgcc aggataccgg cacggccatc gtgctcggca agcgtggcga gctctgctgg accgggggcg aggatgagaa gtacctgtcc aagggtatat ggaacgccta caggtaccac aacctgcgtt acagccagac ggccgcgctg gacatgttca aggagtgcaa taccggtgac aatcttcccg cccagctcga cctgctcgcg gtgcccggaa gcgactacga gttcctcttc attgccaagg gcggcggctc ggcaaacaag gcctacctgt accaggaaac caaggcgctg ctgaacccca aatcgctgcg cgccttcatc gaggagaagc tcaagaccct cggcacggct gcctgcccac cgtaccacat cgccctcgtc atcggcggca cgagcgcgga gatgaccatg aagacggtga agctggccag ctgccgctac tacgactcgc tcccgaccac cggcgacaag tacggccgcg cctttcgcga ccctgagtgg gagaagatcg tgatggaggt ggcgcagaag agcggaattg gagcgcagtt tggtggcaag tacttcgccc accaggcgcg cgtgattcgg ttgccccgcc acggtgcctc ctgcccagtg ggccttgcgg tgtcttgcag cgctgatcgc cagattcttg cgcacatcaa caagagcggc atctacattg agcagcttga gcagaacccg gcgcagtacc tgcccgacat cccggaagtg cacctgagca ccacgagcgt gaaagtcgac ctgaagcgcc ccatcgacaa agtgcgccag cagctcagcc aatacccggt cggtacccgt gttatgctta acggcaccct catcgttgcc cgtgatattg cccacgccaa gatcaaggag atgatggata acggcgagcc gctgccagag tacatgaaga cgtcgcctat ctactacgcc gggcccgcca agacgccgga gggctacgcc tctggttcct ttggcccgac gacggccggc cgcatggact cctacgtcga tctctttcag tcgcacggcg gcagctacat cacgctcgcc aagggcaacc gcagcaagca ggtgacggac gcgtgcaaga agcacggtgg cttctacctc ggcagcattg gcggtccagc ggccatactc gccaaggact cgatcaagca ggtaacatgc ctcgccttcc cggaactcgg catggaggcg gtgtggaaga tcgaggtcga ggacttcccc gcgttcattg tcgtcgatga caagggaaac gacatgtact cgaagacgct tgcgtga
Details for LemaA.18331.a.B1.GE42079
HARVESTED ON: 12/5/2017
SEQUENCED ON: 12/7/2017
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 90
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS LCDQCEIGCR RVGIKDIEDA SAVNADFHFS AIFQPTDPHH HQTEFAKVEG SEKYVEEVEV FGRQALKVNP EALTILAHRA FSDVHHFFRK DHLEGWRRAI EDPEASDNDR YVATTLLKNA CIAAGRVLPS CQDTGTAIVL GKRGELCWTG GEDEKYLSKG IWNAYRYHNL RYSQTAALDM FKECNTGDNL PAQLDLLAVP GSDYEFLFIA KGGGSAXXXY LXXETKALLN PXXLRAFIEE KLKTLGTAAX XXXXXXXVXX XTSXXXXXXT XXXASXXXXX XXXXXXXXXX XXXRXXXWEK IVMEXAQKSG IGAXXXGKYF XXQARVIRLP RHGASCPVGL AVSCSADRQI LAHINKSGIY IEQLEQNPAQ YLPDIPEVHL STTSVKVDLK RPIDKVCQQL SQYPVGTRVM LNGTLIVARD IAHAKIKEMM DNGEPLPEYM KTSPIYYAGP AKTPEGYASG SFGPTTAGRM DSYVDLFQSH GGSYITLAKG NRSKQVTDAC KKHGGFYLGS IGGPAAILAK DSIKQVTCLA FPELGMEAVW KIEVEDFPAF IVVDDKGN
Validated NT Sequence
cgtttccctt gtcatcgacg acaatgaacg cggggaagtc ctcgacctcg atcttccaca ccgcctccat gccgagttcc gggaaggcga ggcatgttac ctgcttgatc gagtccttgg cgagtatggc cgctggaccg ccaatgctgc cgaggtagaa gccaccgtgc ttcttgcacg cgtccgtcac ctgcttgctg cggttgccct tggcgagcgt gatgtagctg ccgccgtgcg actgaaagag atcgacgtag gagtccatgc ggccggccgt cgtcgggcca aaggaaccag aggcgtagcc ctccggcgtc ttggcgggcc cggcgtagta gataggcgac gtcttcatgt actctggcag cggctcgccg ttatccatca tctccttgat cttggcgtgg gcaatatcac gggcaacgat gagggtgccg ttaagcataa cacgggtacc gaccgggtat tggctgagct gctggcacac tttgtcgatg gggcgcttca ggtcgacttt cacgctcgtg gtgctcaggt gcacttccgg gatgtcgggc aggtactgcg ccgggttctg ctcaagctgc tcaatgtaga tgccgctctt gttgatgtgc gcaagaatct ggcgatcagc gctgcaagac accgcaaggc ccactgggca ggaggcaccg tggcggggca accgaatcac gcgcgcctgg tnnncgaagt acttgccann nnnnngcgct ccaattccgc tcttctgcgc cnnctccatc acgatcttct cccactnnnn nnnncgnnnn nngcnnnnnn nnnnnncgcn nnnnnnnnnn nnngnnnnnn nnnnnnnnca gctggccnnn nnnnnngtnn tcnnnnnnnn nnnnncgctc gtnnnnnnnn tgacgnnnnn nnnnnnnnnn nnnnnnnagg cagccgtgcc gagggtcttg agcttctcct cgatgaaggc gcgcagcgnn nnngggttca gcagcgcctt ggtttccnnn tacaggtagn nnnnntttgc cgagccgccg cccttggcaa tgaagaggaa ctcgtagtcg cttccgggca ccgcgagcag gtcgagctgg gcgggaagat tgtcaccggt attgcactcc ttgaacatgt ccagcgcggc cgtctggctg taacgcaggt tgtggtacct gtaggcgttc catataccct tggacaggta cttctcatcc tcgcccccgg tccagcagag ctcgccacgc ttgccgagca cgatggccgt gccggtatcc tggcacgacg gcagcacccg acctgccgcg atgcaggcgt tcttcagcag cgtcgtggcg acgtagcggt cgttgtcgga ggcctcgggg tcctcgatgg cgcggcgcca accctccagg tggtcctttc ggaagaagtg gtgcacatcc gagaaggcgc ggtgggcgag aatagtgagg gcctcggggt tcaccttcag cgcctggcgc ccgaacacct ccacctcctc cacgtacttc tcgctcccct cgaccttggc gaactcggtt tggtgatggt gcgggtcggt gggctggaag atggcggaga agtggaagtc tgcgttgaca gcggacgcgt cctcgatgtc cttgatgccc acacggcggc acccgatctc gcactggtcg cacagagaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS LCDQCEIGCR RVGIKDIEDA SAVNADFHFS AIFQPTDPHH HQTEFAKVEG SEKYVEEVEV FGRQALKVNP EALTILAHRA FSDVHHFFRK DHLEGWRRAI EDPEASDNDR YVATTLLKNA CIAAGRVLPS CQDTGTAIVL GKRGELCWTG GEDEKYLSKG IWNAYRYHNL RYSQTAALDM FKECNTGDNL PAQLDLLAVP GSDYEFLFIA KGGGSANKAY LYQETKALLN PKSLRAFIEE KLKTLGTAAC PPYHIALVIG GTSAEMTMKT VKLASCRYYD SLPTTGDKYG RAFRDPEWEK IVMEVAQKSG IGAQFGGKYF AHQARVIRLP RHGASCPVGL AVSCSADRQI LAHINKSGIY IEQLEQNPAQ YLPDIPEVHL STTSVKVDLK RPIDKVRQQL SQYPVGTRVM LNGTLIVARD IAHAKIKEMM DNGEPLPEYM KTSPIYYAGP AKTPEGYASG SFGPTTAGRM DSYVDLFQSH GGSYITLAKG NRSKQVTDAC KKHGGFYLGS IGGPAAILAK DSIKQVTCLA FPELGMEAVW KIEVEDFPAF IVVDDKGNDM YSKTLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc tgtgcgacca gtgcgagatc gggtgccgcc gtgtgggcat caaggacatc gaggacgcgt ccgctgtcaa cgcagacttc cacttctccg ccatcttcca gcccaccgac ccgcaccatc accaaaccga gttcgccaag gtcgagggga gcgagaagta cgtggaggag gtggaggtgt tcgggcgcca ggcgctgaag gtgaaccccg aggccctcac tattctcgcc caccgcgcct tctcggatgt gcaccacttc ttccgaaagg accacctgga gggttggcgc cgcgccatcg aggaccccga ggcctccgac aacgaccgct acgtcgccac gacgctgctg aagaacgcct gcatcgcggc aggtcgggtg ctgccgtcgt gccaggatac cggcacggcc atcgtgctcg gcaagcgtgg cgagctctgc tggaccgggg gcgaggatga gaagtacctg tccaagggta tatggaacgc ctacaggtac cacaacctgc gttacagcca gacggccgcg ctggacatgt tcaaggagtg caataccggt gacaatcttc ccgcccagct cgacctgctc gcggtgcccg gaagcgacta cgagttcctc ttcattgcca agggcggcgg ctcggcaaac aaggcctacc tgtaccagga aaccaaggcg ctgctgaacc ccaaatcgct gcgcgccttc atcgaggaga agctcaagac cctcggcacg gctgcctgcc caccgtacca catcgccctc gtcatcggcg gcacgagcgc ggagatgacc atgaagacgg tgaagctggc cagctgccgc tactacgact cgctcccgac caccggcgac aagtacggcc gcgcctttcg cgaccctgag tgggagaaga tcgtgatgga ggtggcgcag aagagcggaa ttggagcgca gtttggtggc aagtacttcg cccaccaggc gcgcgtgatt cggttgcccc gccacggtgc ctcctgccca gtgggccttg cggtgtcttg cagcgctgat cgccagattc ttgcgcacat caacaagagc ggcatctaca ttgagcagct tgagcagaac ccggcgcagt acctgcccga catcccggaa gtgcacctga gcaccacgag cgtgaaagtc gacctgaagc gccccatcga caaagtgcgc cagcagctca gccaataccc ggtcggtacc cgtgttatgc ttaacggcac cctcatcgtt gcccgtgata ttgcccacgc caagatcaag gagatgatgg ataacggcga gccgctgcca gagtacatga agacgtcgcc tatctactac gccgggcccg ccaagacgcc ggagggctac gcctctggtt cctttggccc gacgacggcc ggccgcatgg actcctacgt cgatctcttt cagtcgcacg gcggcagcta catcacgctc gccaagggca accgcagcaa gcaggtgacg gacgcgtgca agaagcacgg tggcttctac ctcggcagca ttggcggtcc agcggccata ctcgccaagg actcgatcaa gcaggtaaca tgcctcgcct tcccggaact cggcatggag gcggtgtgga agatcgaggt cgaggacttc cccgcgttca ttgtcgtcga tgacaaggga aacgacatgt actcgaagac gcttgcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at