SSGCID: Target Page

LemaA.18297.a

hypothetical protein, conserved

CENTER ID:	LemaA.18297.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.18297.a.B1.GE40692	full length		1	245

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF21.0040
RefSeq:	XP_001682935.1
UniProt:	Q4QCL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

METMMKIKTA LKLAPSTCDE DYNRRKREMK ELSSAIRSYS TAMEKAKVSV RRLVEALTEA SKAFESLCNY PSIPDSTKDC ALALAASMNR IEDTAFPEFN VAMDSTVLTA TNGLKALYDE CERLEGKRNK IMHEYDAYRE EVAKKEIEYL RKSKSLLKSK YYSTEVARRN ELEAAFESAD QKFKDIHNQL TQSRLVTCTD ALNAFLSCMS RIMEDISGEF EGLKGSSEYV EPVVHNIMCE GYESD

NT Sequence

atggaaacga tgatgaaaat aaaaacggcg ctaaagttgg cgccgtctac ctgcgacgag gattacaacc gccgcaaacg tgaaatgaaa gagttgtcct cggctattcg tagctacagc actgccatgg agaaagcaaa agtgtctgtt aggcgcctcg tagaagcgct caccgaagca agcaaggcat ttgaatcgct gtgcaactac ccaagcatcc cggattccac gaaggattgc gcgctggctc tggcggcctc tatgaatcgt atcgaagata cggcctttcc agagtttaac gtcgcaatgg actcgactgt cctcaccgct acaaatgggc tgaaggcttt gtacgacgag tgcgagaggc tggagggcaa gcgcaacaag attatgcacg agtatgatgc ataccgcgag gaggtcgcaa aaaaagagat agaatacctt cgaaagtcaa agagcttgtt gaagtcgaaa tactatagca ccgaggttgc gagacggaac gagctggagg cggccttcga gtctgcggat cagaaattta aagacatcca caaccaactc acgcaaagcc gtttggtcac atgtacagac gctctcaacg cattcttgag ctgcatgtcg cgtatcatgg aggatatctc cggagaattc gaagggctga aaggctcttc ggagtatgtg gagccagtag tgcacaatat aatgtgtgaa gggtacgaaa gcgactga

Details for LemaA.18297.a.B1.GE40692

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	29kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME TMMKIKTALK LAPSTCDEDY NRRKREMKEL SSAIRSYSTA MEKAKVSVRR LVEALTEASK AFESLCNYPS IPDSTKDCAL ALAASMNRIE DTAFPEFNVA MDSTVLTATN GLKALYDECE RLEGKRNKIM HEYDAYREEV AKKEIEYLRK SKSLLKSKYY STEVARRNEL EAAFESADQK FKDIHNQLTQ SRLVTCTDAL NAFLSCMSRI MEDISGEFEG LKGSSEYVEP VVHNIMCXGY ESD

Validated NT Sequence

atggctcacc accaccacca ccatatggaa acgatgatga aaataaaaac ggcgctaaag ttggcgccgt ctacctgcga cgaggattac aaccgccgca aacgtgaaat gaaagagttg tcctcggcta ttcgtagcta cagcactgcc atggagaaag caaaagtgtc tgttaggcgc ctcgtagaag cgctcaccga agcaagcaag gcatttgaat cgctgtgcaa ctacccaagc atcccggatt ccacgaagga ttgcgcgctg gctctggcgg cctctatgaa tcgtatcgaa gatacggcct ttccagagtt taacgtcgca atggactcga ctgtcctcac cgctacaaat gggctgaagg ctttgtacga cgagtgcgag aggctggagg gcaagcgcaa caagattatg cacgagtatg atgcataccg cgaggaggtc gcaaaaaaag agatagaata ccttcgaaag tcaaagagct tgttgaagtc gaaatactat agcaccgagg ttgcgagacg gaacgagctg gaggcggcct tcgagtctgc ggatcagaaa tttaaagaca tccacaacca actcacgcaa agccgtttgg tcacatgtac agacgctctc aacgcattct tgagctgcat gtcgcgtatc atggaggata tctccggaga attcgaaggg ctgaaaggct cttcggagta tgtggagcca gtagtgcaca atataatgtg tgnagggtac gaaagcgact aagtgagtaa gat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa cgatgatgaa aataaaaacg gcgctaaagt tggcgccgtc tacctgcgac gaggattaca accgccgcaa acgtgaaatg aaagagttgt cctcggctat tcgtagctac agcactgcca tggagaaagc aaaagtgtct gttaggcgcc tcgtagaagc gctcaccgaa gcaagcaagg catttgaatc gctgtgcaac tacccaagca tcccggattc cacgaaggat tgcgcgctgg ctctggcggc ctctatgaat cgtatcgaag atacggcctt tccagagttt aacgtcgcaa tggactcgac tgtcctcacc gctacaaatg ggctgaaggc tttgtacgac gagtgcgaga ggctggaggg caagcgcaac aagattatgc acgagtatga tgcataccgc gaggaggtcg caaaaaaaga gatagaatac cttcgaaagt caaagagctt gttgaagtcg aaatactata gcaccgaggt tgcgagacgg aacgagctgg aggcggcctt cgagtctgcg gatcagaaat ttaaagacat ccacaaccaa ctcacgcaaa gccgtttggt cacatgtaca gacgctctca acgcattctt gagctgcatg tcgcgtatca tggaggatat ctccggagaa ttcgaagggc tgaaaggctc ttcggagtat gtggagccag tagtgcacaa tataatgtgt gaagggtacg aaagcgactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat