LemaA.18295.a

arginyl-tRNA synthetase, putative

CENTER ID: LemaA.18295.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18295.a.B1.GE42689 full length 1 692
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.27.1310
UniProt: E9ADB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCAAAATVNV ELALKEIVKA TIAKTFPLVP TQEPLITVGK VSEYQCNNAM GLVKVLSQQQ QPIKMSPQMV GEELKKNLVD NDIIDEFEPT SKGFINVSIR KEWLARMVKE VLKQGIRPPV VKKQRVLVDF SAPNIAKEMH VGHLRSTIIG ESISRLLEFC QHDVARINHI GDWGTAFGML VLYIKRNFPD YTTNPPDISD LTAFYRAAKK CFDEDQEFKE KARLEVVKLQ ALEEDSIQAW KYICDVSREE FSKVYTRLGA TIEERGESFY NPLIPKVLGL LEEAGQIEVS DGAKLIVSKE ERKLCSLDAR DMTKLASQHL ALVSRDGPSY HPNLIAAMKK AGILTGAEGE ESVALSKKEV KPWKKFDIRV DLDKLMAQLA PLYKKQLDPL FLEVFEAAGI VKGDSILVPR FSFPLMVVKS DGGYTYDTTD VTATYHRFVI EKMNRVIYCT DLGQYEHFRM CLQTAKDMGW MEHATWDHAG FGLVTGADGK KIKTRSGETV KLKDLMDEAV ERSLAILKER EAGERSQGHS EEVMQKLSNI IGIGAIKYFD LKQTRTGDYA FSYDKMLDMS GNTAVFLLYQ YARICSIQRK AGIADEELFK ITDISLETPQ EKSLALCALR FQTVILKTVE DLFPHHLADF AYELMTNFSN FFQNCRVLDD PLQNSRLCLV ELTRITLKKT LELLNIETAE RI
NT Sequence
atgtgcgccg ctgccgctac cgtgaatgtg gagctggcgc tgaaggagat tgtcaaggcg accattgcca agaccttccc gctcgtcccg acccaggagc ctctcatcac cgttggcaag gtgagcgagt atcagtgcaa caatgccatg gggctggtga aagtgctctc tcagcagcag cagccgataa agatgagccc gcagatggtc ggcgaggagc tcaagaagaa cctggtggac aatgatatca tcgacgagtt cgaacccacg tccaagggtt tcatcaatgt cagcatccgc aaagaatggt tggctaggat ggtgaaagag gtgctgaaac agggcatccg cccacccgtc gtgaagaagc agcgcgtgct cgtcgacttt tcagcgccga acattgccaa ggaaatgcat gtcggacatc tccgctccac catcatcggc gagtcgatca gccgcctgct ggagttctgc cagcatgacg ttgcacgcat caaccacatc ggtgactggg gtacggcgtt tggcatgctc gttctgtaca tcaagcgcaa tttccccgac tacaccacga acccgccgga catctccgac ctcaccgcct tctaccgcgc ggcgaagaag tgcttcgacg aggaccagga gttcaaggag aaggcccgcc tcgaggtggt gaagctgcag gcgctggagg aggattctat ccaagcgtgg aagtacatct gcgacgtgtc acgcgaggag ttcagcaagg tctacacccg cctcggcgcc accatcgagg agcggggcga gagcttctac aacccgctta ttcccaaggt gctcggtctg ctggaggagg caggacagat cgaggtgagt gacggcgcaa agctcatcgt cagcaaggag gagcgcaaac tgtgtagcct cgatgcgcgt gacatgacaa agctggcgag ccagcacctc gcgctcgtct cgcgcgatgg cccgtcgtac cacccgaacc tgattgccgc catgaagaag gctggcatcc tgaccggggc tgagggtgag gagtcggtgg cgctgtcgaa gaaggaggtg aagccgtgga aaaagttcga tatccgcgtg gacctggaca agctgatggc ccagctcgca cccctgtaca agaagcagct cgatcctctc ttcctggagg tgttcgaggc tgcggggatt gtcaagggcg acagcatcct tgtcccccgt ttctccttcc cgctgatggt ggtgaagagt gatgggggct acacctacga cacgacagat gtcacggcca cgtaccaccg cttcgtcatc gagaagatga accgagtcat ctactgcact gatttgggcc agtacgagca cttccgcatg tgtctacaga cggcgaagga catgggctgg atggaacacg ctacatggga ccacgccggc tttggccttg tgacgggtgc ggatggcaag aagataaaga cgcgctcggg tgagacggtg aagctgaaag acctcatgga cgaggcggtg gagcggtccc ttgcgatcct gaaggagcgt gaggcgggcg agcgcagcca aggccattcg gaggaagtga tgcagaagct gtcgaacatt atcggcattg gcgccatcaa gtacttcgat ctcaagcaga cgcgcactgg tgactacgcc ttcagctacg acaagatgtt ggacatgtct ggaaacacgg ccgtgttcct tctctatcag tacgcccgca tttgctctat ccagcgcaag gccggcatag cggatgagga gctgttcaag atcaccgaca tctctctcga gaccccgcag gagaagagtc tcgcgctgtg cgcgctgcgc ttccagacgg tgattctcaa gaccgtcgag gacctcttcc cgcaccacct ggcggacttc gcgtacgagc tcatgacaaa cttctccaac tttttccaaa actgccgcgt cttggatgac ccgctgcaga acagccgcct gtgcctagtt gagctcaccc gcatcaccct caagaagaca ttggagctgc tgaacatcga gacggccgag cgcatttag
Details for LemaA.18295.a.B1.GE42689
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 79kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMC AAAATVNVEL ALKEIVKATI AKTFPLVPTQ EPLITVGKVS EYQCNNAMGL VKVLSQQQQP IKMSPQMVGE ELKKNLVDND IIDEFEPTSK GFINVSIRKE WLARMVKEVL KQGIRPPVVK KQRVLVDFSA PNIAKEMHVG HLRSTIIGES ISRLLEFCQH DVARINHIGD WGTAFGMLVL YIKRNFPDYT TNPPDISDLT AFYRAAKKCF DEDQEFKEKA RLEVVKLQAL EEDSIQAWKY ICDVSREEFS KVYTRLGATI EERGESFYNP LIPKVLGLLE EAGQIEVSDG AKLIVSKEER KLCSLDARDM TKLASQHLAL VSRDGPSYHP NLIAAMKKAG ILTGAEGEES VALSKKEVKP WKKFDIRVDL DKLMAQLAPL YKKQLDPLFL EVFEAAGIVK GDSILVPRFS FPLMVVKSDG GYTYDTTDVT ATYHRFVIEK MNRVIYCTDL GQYEHFRMCL QTAKDMGWME HATWDHAGFG LVTGADGKKI KTRSGETVKL KDLMDEAVER SLAILKEREA GERSQGHSEE VMQKLSNIIG IGAIKYFDLK QTRTGDYAFS YDKMLDMSGN TAVFLLYQYA RICSIQRKAG IADEELFKIT DISLETPQEK SLALCALRFQ TVILKTVEDL FPHHLADFAY ELMTNFSNFF QNCRVLDDPL QNSRLCLVEL TRITLKKTLE LLNIETAERI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgcg ccgctgccgc taccgtgaat gtggagctgg cgctgaagga gattgtcaag gcgaccattg ccaagacctt cccgctcgtc ccgacccagg agcctctcat caccgttggc aaggtgagcg agtatcagtg caacaatgcc atggggctgg tgaaagtgct ctctcagcag cagcagccga taaagatgag cccgcagatg gtcggcgagg agctcaagaa gaacctggtg gacaatgata tcatcgacga gttcgaaccc acgtccaagg gtttcatcaa tgtcagcatc cgcaaagaat ggttggctag gatggtgaaa gaggtgctga aacagggcat ccgcccaccc gtcgtgaaga agcagcgcgt gctcgtcgac ttttcagcgc cgaacattgc caaggaaatg catgtcggac atctccgctc caccatcatc ggcgagtcga tcagccgcct gctggagttc tgccagcatg acgttgcacg catcaaccac atcggtgact ggggtacggc gtttggcatg ctcgttctgt acatcaagcg caatttcccc gactacacca cgaacccgcc ggacatctcc gacctcaccg ccttctaccg cgcggcgaag aagtgcttcg acgaggacca ggagttcaag gagaaggccc gcctcgaggt ggtgaagctg caggcgctgg aggaggattc tatccaagcg tggaagtaca tctgcgacgt gtcacgcgag gagttcagca aggtctacac ccgcctcggc gccaccatcg aggagcgggg cgagagcttc tacaacccgc ttattcccaa ggtgctcggt ctgctggagg aggcaggaca gatcgaggtg agtgacggcg caaagctcat cgtcagcaag gaggagcgca aactgtgtag cctcgatgcg cgtgacatga caaagctggc gagccagcac ctcgcgctcg tctcgcgcga tggcccgtcg taccacccga acctgattgc cgccatgaag aaggctggca tcctgaccgg ggctgagggt gaggagtcgg tggcgctgtc gaagaaggag gtgaagccgt ggaaaaagtt cgatatccgc gtggacctgg acaagctgat ggcccagctc gcacccctgt acaagaagca gctcgatcct ctcttcctgg aggtgttcga ggctgcgggg attgtcaagg gcgacagcat ccttgtcccc cgtttctcct tcccgctgat ggtggtgaag agtgatgggg gctacaccta cgacacgaca gatgtcacgg ccacgtacca ccgcttcgtc atcgagaaga tgaaccgagt catctactgc actgatttgg gccagtacga gcacttccgc atgtgtctac agacggcgaa ggacatgggc tggatggaac acgctacatg ggaccacgcc ggctttggcc ttgtgacggg tgcggatggc aagaagataa agacgcgctc gggtgagacg gtgaagctga aagacctcat ggacgaggcg gtggagcggt cccttgcgat cctgaaggag cgtgaggcgg gcgagcgcag ccaaggccat tcggaggaag tgatgcagaa gctgtcgaac attatcggca ttggcgccat caagtacttc gatctcaagc agacgcgcac tggtgactac gccttcagct acgacaagat gttggacatg tctggaaaca cggccgtgtt ccttctctat cagtacgccc gcatttgctc tatccagcgc aaggccggca tagcggatga ggagctgttc aagatcaccg acatctctct cgagaccccg caggagaaga gtctcgcgct gtgcgcgctg cgcttccaga cggtgattct caagaccgtc gaggacctct tcccgcacca cctggcggac ttcgcgtacg agctcatgac aaacttctcc aactttttcc aaaactgccg cgtcttggat gacccgctgc agaacagccg cctgtgccta gttgagctca cccgcatcac cctcaagaag acattggagc tgctgaacat cgagacggcc gagcgcattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat