LemaA.18236.a

KREL2

CENTER ID: LemaA.18236.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18236.a.B1.GE40691 full length 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LmjF.20.1730
RefSeq: XP_001682919.1
UniProt: Q6T452

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRCLLTRL VRRSLVLFSD EKEALFERYS EIENAHSRRI EALKDAGLFN DEWIATEKVH GANFGIYSTE HGKTIQYAKR SGIMPPYEHF FGYHILIPDL ARYAKQGREL LTEQLGMSPS TVIINGELFG GKYDHPSVPK RRQNVMVAGR PRTITAVQNN AFPQYCPDLH FYAFDIKYRK TEEEEYCTMT YDQALAIFEK ISGLLYAKAI IRGPLSKVAA FDVENFVTTI PPLVGMGDYP LKGNWAEGLV VRHCRRGEPD FDAKGLTIMK FKCTAFQEIS TDRRQGPRVD ALEEVRRQSI KVSGVQLPDM ESVIGDPEVL QAAHRLLDHV CENRLQSVLS KIGTGPFETQ SMTPTELSTL LAKDALKDFL KEADAKIVNT PLLVRREMVR YVVFEARKYV ASRWKDVVGQ QKEADGAS
NT Sequence
atgcttcggc gatgcctgct tacacggctc gtgcgtcgct cgctggtcct cttttcggac gaaaaagaag cgctgttcga gcgctatagc gagattgaaa acgcccactc acgacgcatt gaagcactga aggacgcggg tctcttcaat gatgagtgga ttgccacgga gaaggtgcat ggcgccaact tcggcatcta ctcaacagag catgggaaga ctattcagta cgcgaagcgg agcggcatta tgccgccgta cgagcacttt tttggctacc acatcctcat acctgatctc gcgaggtatg ccaagcaagg gcgagaactg cttacagaac agctcggcat gtcacccagc accgtaatca tcaacggtga gctgtttggt ggcaagtatg accatccaag cgtgccgaag cggcggcaga acgtgatggt ggccggtagg ccacgcacca tcacggctgt gcagaacaat gcctttcctc agtactgccc agacctgcat ttctatgcct ttgacatcaa gtaccggaag accgaggagg aggagtactg caccatgacg tacgaccaag ccttggccat cttcgagaaa atttcgggcc tgctctacgc gaaggctatc attcgagggc cgctgagcaa ggtggccgcg ttcgacgtgg aaaacttcgt cacgacaatc ccgccgctgg tgggcatggg cgactacccg ctcaagggga actgggcgga gggcctcgtt gtaagacact gccgtcgcgg cgagcctgac ttcgacgcaa agggcctcac catcatgaag ttcaagtgca ccgccttcca ggagatctcg accgaccgcc gccagggacc gcgcgtagac gcgctggagg aggttcggcg gcagtccatc aaagtctccg gtgtacagct gccggacatg gagagcgtca tcggcgaccc cgaggtgctc caagcggcgc atcgcctgct cgaccacgtg tgtgagaatc gccttcagag tgtcttgtcc aagatcggta caggcccgtt cgagacgcag tccatgaccc ccacagagct ctccacgctg ctcgcaaagg atgcgctgaa agacttcttg aaggaggcgg atgcaaaaat cgtcaacacg ccgctactcg tgcgccgcga gatggtccgc tacgttgttt ttgaggcgcg caagtacgtg gccagcagat ggaaggacgt cgttgggcag caaaaggagg cagacggggc gtcgtga
Details for LemaA.18236.a.B1.GE40691
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 76
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHML RRCLLTRLVR RSLVLFSDEK EALFERYSEI ENAHSRRIEA LKDAGLFNDE WIATEKVHGA NFGIYSTEHG KTIQYAKRSG IMPPYEHFFG YHILIPDLAR YAKQGRELLT EQLGMSPSTV IINGELFGGK YDHPSVPKRR QNVMVAGRPR TITAVQNNAF PQYCPDLHFY AFDIKYRKTE EEEYCTMTYD QALAIFEKIS GLLYAKAIIR GPLSKVAAFD VENFVTTIPP LVGMGDYPLX XXWAEGLVVR HCRRGEPDXX XKGPHHHEVQ VHRLPGDLDX XXXXXXAXXR WRRXXXSPSK XXXXXXRXXR ASXXXXRXXX XXXXXXXXXX XXXXRVXXPX XXXXXXXXXP
Validated NT Sequence
atggctcacc accaccacca ccatatgctt cggcgatgcc tgcttacacg gctcgtgcgt cgctcgctgg tcctcttttc ggacgaaaaa gaagcgctgt tcgagcgcta tagcgagatt gaaaacgccc actcacgacg cattgaagca ctgaaggacg cgggtctctt caatgatgag tggattgcca cggagaaggt gcatggcgcc aacttcggca tctactcaac agagcatggg aagactattc agtacgcgaa gcggagcggc attatgccgc cgtacgagca cttttttggc taccacatcc tcatacctga tctcgcgagg tatgccaagc aagggcgaga actgcttaca gaacagctcg gcatgtcacc cagcaccgta atcatcaacg gtgagctgtt tggtggcaag tatgaccatc caagcgtgcc gaagcggcgg cagaacgtga tggtggccgg taggccacgc accatcacgg ctgtgcagaa caatgccttt cctcagtact gcccagacct gcatttctat gcctttgaca tcaagtaccg gaagaccgag gaggaggagt actgcaccat gacgtacgac caagccttgg ccatcttcga gaaaatttcg ggcctgctct acgcgaaggc tatcattcga gggccgctga gcaaggtggc cgcgttcgac gtggaaaact tcgtcacgac aatcccgccg ctggtgggca tgggcgacta cccgctcaan nnnnnntggg cggagggcct cgttgtaaga cactgccgtc gcggcgagcc tgactnnnnn cncaaagggc ctcaccatca tgaagttcaa gtgcaccgcc ttccaggaga tctcgacnnn ncnnnnnnnn nnannnncgc gnngnngcgc tggaggaggt nnnnnnnnag tccatcaaag tnnnnnnnnn ncnnnnnncg nnnanggaga gcgtcannnn ngnnncngag gnnnncnnnn nnnngcannn nnnnnnnnnn nnnnngnnnn nnnannnnnn ncagagtgnn nnnnccaann nnnnnnnnnn ncnnnnnnnn nnnnngtcca tgaccnncac agagcnnncc annnngnncn nnnaggatgc gnnnaaagac nnnnnnaagg agnnngnngn aaaannnnnc nnnannnngn nncnnnnncn cnnnnagnnn ntcnnnnncg ttgnnnnnnn ngnnnnnaag nnnnnnnncn nnnnnnngna ggnnnnnnnn nngnannnna
Expected Protein Sequence
MAHHHHHHML RRCLLTRLVR RSLVLFSDEK EALFERYSEI ENAHSRRIEA LKDAGLFNDE WIATEKVHGA NFGIYSTEHG KTIQYAKRSG IMPPYEHFFG YHILIPDLAR YAKQGRELLT EQLGMSPSTV IINGELFGGK YDHPSVPKRR QNVMVAGRPR TITAVQNNAF PQYCPDLHFY AFDIKYRKTE EEEYCTMTYD QALAIFEKIS GLLYAKAIIR GPLSKVAAFD VENFVTTIPP LVGMGDYPLK GNWAEGLVVR HCRRGEPDFD AKGLTIMKFK CTAFQEISTD RRQGPRVDAL EEVRRQSIKV SGVQLPDMES VIGDPEVLQA AHRLLDHVCE NRLQSVLSKI GTGPFETQSM TPTELSTLLA KDALKDFLKE ADAKIVNTPL LVRREMVRYV VFEARKYVAS RWKDVVGQQK EADGAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc ggcgatgcct gcttacacgg ctcgtgcgtc gctcgctggt cctcttttcg gacgaaaaag aagcgctgtt cgagcgctat agcgagattg aaaacgccca ctcacgacgc attgaagcac tgaaggacgc gggtctcttc aatgatgagt ggattgccac ggagaaggtg catggcgcca acttcggcat ctactcaaca gagcatggga agactattca gtacgcgaag cggagcggca ttatgccgcc gtacgagcac ttttttggct accacatcct catacctgat ctcgcgaggt atgccaagca agggcgagaa ctgcttacag aacagctcgg catgtcaccc agcaccgtaa tcatcaacgg tgagctgttt ggtggcaagt atgaccatcc aagcgtgccg aagcggcggc agaacgtgat ggtggccggt aggccacgca ccatcacggc tgtgcagaac aatgcctttc ctcagtactg cccagacctg catttctatg cctttgacat caagtaccgg aagaccgagg aggaggagta ctgcaccatg acgtacgacc aagccttggc catcttcgag aaaatttcgg gcctgctcta cgcgaaggct atcattcgag ggccgctgag caaggtggcc gcgttcgacg tggaaaactt cgtcacgaca atcccgccgc tggtgggcat gggcgactac ccgctcaagg ggaactgggc ggagggcctc gttgtaagac actgccgtcg cggcgagcct gacttcgacg caaagggcct caccatcatg aagttcaagt gcaccgcctt ccaggagatc tcgaccgacc gccgccaggg accgcgcgta gacgcgctgg aggaggttcg gcggcagtcc atcaaagtct ccggtgtaca gctgccggac atggagagcg tcatcggcga ccccgaggtg ctccaagcgg cgcatcgcct gctcgaccac gtgtgtgaga atcgccttca gagtgtcttg tccaagatcg gtacaggccc gttcgagacg cagtccatga cccccacaga gctctccacg ctgctcgcaa aggatgcgct gaaagacttc ttgaaggagg cggatgcaaa aatcgtcaac acgccgctac tcgtgcgccg cgagatggtc cgctacgttg tttttgaggc gcgcaagtac gtggccagca gatggaagga cgtcgttggg cagcaaaagg aggcagacgg ggcgtcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at