LemaA.18222.b

glutaminyl-tRNA synthetase, putative

CENTER ID: LemaA.18222.b
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18222.b.B1.GE42688 full length 1 570
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.15.1440
UniProt: Q4QF36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGPAAAEVK EMETKRDLSI LQSGRPVPGC RNTRELLETH EKVTGGKPYF RFPPEPNGFL HIGHAKSMNL NFGSARAHGG KCYLRYDDTN PESEDQVYID AIMEMVNWMG WKPDWVTFSS DYFDQLHTFA VQLIKDGKAY VDHSTPDELK QQREQREDSP WRNRSVEENL LLFKHMRQGR YAEGEATLRV KADMKSDNPN MRDFIAYRVK YVEHPHVKDK WCIYPSYDFT HCLIDSLEDI DYSLCTLEFE TRRESYFWLL NELNLWRPHV WEFSRLNVTG SLLSKRKINV LVRKGIVRGF DDPRLLTLAG MRRRGYTPAA INRFCELVGI TRSMNVIQIS MLENTLREDL DERCERRLMV IDPIKVVVDN WKGERIFECP NHPRKPELGG RALTFTDTFY VDRSDFRTED NNNKFYGLAP GPRVVGLKYS GNVVCKSFEV DANGQSMVIH VDIDFERKDK PKTNISWVSA TACTPVEVRL YNALLKDDRA AIDPEFLKFI DENSEVVSHG YAEKGVENFK HFESIQVERF GYFVVDPDTK VDHLVMNRVL GLREDKSKAT VGEPVPTKRK
NT Sequence
atggcgggac cggcagctgc ggaagtgaaa gaaatggaga cgaagcgcga cctgtcgatc ctgcagagcg gccgtcccgt cccggggtgc cggaacacgc gcgagctgct cgagacgcac gagaaggtca ccggcgggaa gccgtacttt cgctttcctc cggagccaaa tggcttcctg cacattggcc atgccaagag catgaacctc aacttcggca gcgcacgcgc gcacggtggc aagtgctacc tgcgctacga cgatacgaac cccgagtcgg aggatcaggt ctacattgat gctatcatgg agatggtgaa ctggatgggg tggaaaccgg actgggtcac cttttcctcc gactacttcg accagctgca caccttcgcc gtgcagctca tcaaggatgg gaaggcctac gtcgatcaca gcacgccaga cgagctgaag cagcagcggg agcagcgcga ggacagcccg tggcgcaacc gcagcgtcga ggagaatctg ctcctcttca agcacatgcg ccagggccgt tacgctgagg gcgaggcgac gcttcgcgtg aaggccgata tgaagagcga caacccgaac atgcgcgact tcattgcgta ccgcgtcaag tacgtcgagc acccgcacgt caaggacaag tggtgcatct acccaagcta cgacttcacc cactgcctca tcgactcgct cgaggacatc gactacagcc tgtgtacgct ggagttcgag acgcgccgcg agagctactt ctggctgctg aacgagctca acctgtggcg tccgcacgtg tgggagttca gccgcctgaa cgtgacgggc tcgctgctct cgaagcgtaa gatcaacgtg ctggtgcgca agggcattgt ccgcgggttt gacgaccccc gtctgctcac ccttgccggc atgcgtcgcc gcgggtacac tccggccgcc attaaccgct tctgcgagct ggttggtatc acccgctcca tgaatgtaat tcagatcagc atgctggaga acaccctgcg cgaggacctc gacgagcgct gcgagcgccg gctgatggtg attgacccaa tcaaagtggt ggtggacaac tggaagggcg agcgaatctt cgagtgcccg aaccatccgc gcaagcctga gctcggtggc cgcgccttga catttacgga caccttctac gtcgaccgca gcgacttccg cacagaagac aacaacaaca agttctacgg actcgcccct ggcccacgcg tggtgggcct caagtactca ggcaacgttg tgtgcaagag cttcgaggtg gacgccaatg gacagtcgat ggtgattcat gtagacatcg acttcgagcg caaggacaag ccgaagacaa atatctcctg ggtgagtgcg acggcatgta cgccggtgga ggtgcgcttg tacaacgcgc tcctcaagga cgaccgtgcc gccatcgatc ccgagtttct caagttcatc gacgagaaca gtgaggtggt gtcgcacggg tacgcggaga agggcgtcga gaatttcaag cacttcgagt cgatccaggt ggaacgcttc gggtacttcg tcgtcgaccc tgatacgaag gtcgaccacc tcgtcatgaa ccgagtgctg ggcctgcgcg aggacaaaag caaggccacc gttggcgagc cggtgcctac gaagaggaag tag
Details for LemaA.18222.b.B1.GE42688
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 79
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHMA GPAAAXXKEM ETKRDLSILQ SGRPVPGCRN TRELLETHEK VTGGKPYFRF PPEPNGFLHI GHAKSMNLNF GSARAHGGKC YLRYDDTNPE SEDQVYIDAI MEMVNWMGWK PDWVTFSSDY FDQLHTFAVQ LIKDGKAYVD HSTPDELKQQ REQREDSPWR NRXVEENLLL FKHMRQGRYA EGEATLRXKA DMKSDNPNMX XFIAYRVKYX XHPHXXDKWC IYPSYDFTHC LIDSLXDIDX XXCTXXXXTR XXXXXWLXXX XXXXXXXXWX XXXXXXXXXX XXXXKXXXXX XXGIXXXFDD PRLLTLXXXA SPRVHSGRH
Validated NT Sequence
tcgccaacgg tggnnttgct tttgtcctcg cgcaggccca gcactcggtt catgacgagg tggtcgacct tcgtatcagg gtcgacgacg aagtacccga agcgttccac ctggatcgac tcgaagtgct tgaaattctc gacgcccttc tccgcgtacc cgtgcgacac cacctcactg ttctcgtcga tgaacttgag aaactcggga tcgatggcgg cacggtcgtc cttgaggagc gcgttgtaca agcgcacctc caccggcgta catgccgtcg cactcaccca ggagatattt gtcttnggct tgtccttgcg ctcgaagtcg atgtctacat gaatcaccat cgactgtcca ttggcgtcca cctcgaagct cttgcacann ncgttgcctg agtacttgag gcccaccacg cgtgggccag gggcgagtcc gtagaacttg ttgttgttgt cttctgtgcg gaagtcgctg cggtcgacgt nnnaggtgtc cgtaaatgtc aaggcgcggc caccgagctc nnncttgcgc ggatggttcg ggcactcgaa gattnnntcg cccttccagt tgtccaccac cactttgatt gggtcaatca ccatcagccg gcgctcgcag cgctcgtcga ggtcctcgnn nagggtgttc tccagcatgc tgatctgaat tacattcatg gagcgggtga taccaaccag ctcgcnnnnn cggttaatgg cggccggagt gtacccgcgg cgacgcangn nnnnnaaggg tgagcagacg ggggtcgtca aaccnnnnnn caatgccctn nnnnnnnnnn annnnnatct tannnnnnnn nngnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccac ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcagccagn nnnnnntcnn nngncgcgtn nnnnnnnnnn nngtacacnn nnnntagtcg atgtcctnna gcgagtcgat gaggcagtgg gtgaagtcgt agcttgggta gatgcaccac ttgtccnnnn ngtgcgggtg ctnnnngtac ttgacgcggt acgcaatgaa gtnnnncatg ttcgggttgt cgctcttcat atcggccttc ncncgaagcg tcgcctcgcc ctcagcgtaa cggccctggc gcatgtgctt gaagaggagc agattctcct cgacgnngcg gttgcgccac gggctgtcct cgcgctgctc ccgctgctgc ttcagctcgt ctggcgtgct gtgatcgacg taggccttcc catccttgat gagctgcacg gcgaaggtgt gcagctggtc gaagtagtcg gaggaaaagg tgacccagtc cggtttccac cccatccagt tcaccatctc catgatagca tcaatgtaga cctgatcctc cgactcgggg ttcgtatcgt cgtagcgcag gtagcacttg ccaccgtgcg cgcgtgcgct gccgaagttg aggttcatgc tcttggcatg gccaatgtgc aggaagccat ttggctccgg aggaaagcga aagtacggct tcccgccggt gaccttctcg tgcgtctcga gcagctcgcg cgtgttccgg caccccggga cgggacggcc gctctgcagg atcgacaggt cgcgcttcgt ctccatttct ttcannnccg cagctgccgg tcccgccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA GPAAAEVKEM ETKRDLSILQ SGRPVPGCRN TRELLETHEK VTGGKPYFRF PPEPNGFLHI GHAKSMNLNF GSARAHGGKC YLRYDDTNPE SEDQVYIDAI MEMVNWMGWK PDWVTFSSDY FDQLHTFAVQ LIKDGKAYVD HSTPDELKQQ REQREDSPWR NRSVEENLLL FKHMRQGRYA EGEATLRVKA DMKSDNPNMR DFIAYRVKYV EHPHVKDKWC IYPSYDFTHC LIDSLEDIDY SLCTLEFETR RESYFWLLNE LNLWRPHVWE FSRLNVTGSL LSKRKINVLV RKGIVRGFDD PRLLTLAGMR RRGYTPAAIN RFCELVGITR SMNVIQISML ENTLREDLDE RCERRLMVID PIKVVVDNWK GERIFECPNH PRKPELGGRA LTFTDTFYVD RSDFRTEDNN NKFYGLAPGP RVVGLKYSGN VVCKSFEVDA NGQSMVIHVD IDFERKDKPK TNISWVSATA CTPVEVRLYN ALLKDDRAAI DPEFLKFIDE NSEVVSHGYA EKGVENFKHF ESIQVERFGY FVVDPDTKVD HLVMNRVLGL REDKSKATVG EPVPTKRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gaccggcagc tgcggaagtg aaagaaatgg agacgaagcg cgacctgtcg atcctgcaga gcggccgtcc cgtcccgggg tgccggaaca cgcgcgagct gctcgagacg cacgagaagg tcaccggcgg gaagccgtac tttcgctttc ctccggagcc aaatggcttc ctgcacattg gccatgccaa gagcatgaac ctcaacttcg gcagcgcacg cgcgcacggt ggcaagtgct acctgcgcta cgacgatacg aaccccgagt cggaggatca ggtctacatt gatgctatca tggagatggt gaactggatg gggtggaaac cggactgggt caccttttcc tccgactact tcgaccagct gcacaccttc gccgtgcagc tcatcaagga tgggaaggcc tacgtcgatc acagcacgcc agacgagctg aagcagcagc gggagcagcg cgaggacagc ccgtggcgca accgcagcgt cgaggagaat ctgctcctct tcaagcacat gcgccagggc cgttacgctg agggcgaggc gacgcttcgc gtgaaggccg atatgaagag cgacaacccg aacatgcgcg acttcattgc gtaccgcgtc aagtacgtcg agcacccgca cgtcaaggac aagtggtgca tctacccaag ctacgacttc acccactgcc tcatcgactc gctcgaggac atcgactaca gcctgtgtac gctggagttc gagacgcgcc gcgagagcta cttctggctg ctgaacgagc tcaacctgtg gcgtccgcac gtgtgggagt tcagccgcct gaacgtgacg ggctcgctgc tctcgaagcg taagatcaac gtgctggtgc gcaagggcat tgtccgcggg tttgacgacc cccgtctgct cacccttgcc ggcatgcgtc gccgcgggta cactccggcc gccattaacc gcttctgcga gctggttggt atcacccgct ccatgaatgt aattcagatc agcatgctgg agaacaccct gcgcgaggac ctcgacgagc gctgcgagcg ccggctgatg gtgattgacc caatcaaagt ggtggtggac aactggaagg gcgagcgaat cttcgagtgc ccgaaccatc cgcgcaagcc tgagctcggt ggccgcgcct tgacatttac ggacaccttc tacgtcgacc gcagcgactt ccgcacagaa gacaacaaca acaagttcta cggactcgcc cctggcccac gcgtggtggg cctcaagtac tcaggcaacg ttgtgtgcaa gagcttcgag gtggacgcca atggacagtc gatggtgatt catgtagaca tcgacttcga gcgcaaggac aagccgaaga caaatatctc ctgggtgagt gcgacggcat gtacgccggt ggaggtgcgc ttgtacaacg cgctcctcaa ggacgaccgt gccgccatcg atcccgagtt tctcaagttc atcgacgaga acagtgaggt ggtgtcgcac gggtacgcgg agaagggcgt cgagaatttc aagcacttcg agtcgatcca ggtggaacgc ttcgggtact tcgtcgtcga ccctgatacg aaggtcgacc acctcgtcat gaaccgagtg ctgggcctgc gcgaggacaa aagcaaggcc accgttggcg agccggtgcc tacgaagagg aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat