LemaA.18219.a

Glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) (N-myristoyl transferase) (NMT)

CENTER ID: LemaA.18219.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18219.a.B2.GE42451 snsd 6 421
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
6OMK
DEPOSITED: 4/18/2019
DETERMINATION: XRay
CLONE: LemaA.18219.a.B2.GE42451
PROTEIN: LemaA.18219.a.B2.PS38467
8FI4
DEPOSITED: 12/15/2022
DETERMINATION: XRay
CLONE: LemaA.18219.a.B2.GE42451
PROTEIN: LemaA.18219.a.B2.PS38707
8FI5
DEPOSITED: 12/15/2022
DETERMINATION: XRay
CLONE: LemaA.18219.a.B2.GE42451
PROTEIN: LemaA.18219.a.B2.PS38707
8FI6
DEPOSITED: 12/15/2022
DETERMINATION: XRay
CLONE: LemaA.18219.a.B2.GE42451
PROTEIN: LemaA.18219.a.B2.PS38707
External Resources
RESOURCE REFERENCE ID
EuPathDB: LmjF.32.0080
UniProt: Q9GPZ4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRNPSNSDA AHAFWSTQPV PQTEDETEKI VFAGPMDEPK TVADIPEEPY PIASTFEWWT PNMEAADDIH AIYELLRDNY VEDDDSMFRF NYSEEFLQWA LCPPNYIPDW HVAVRRKADK KLLAFIAGVP VTLRMGTPKY MKVKAQEKGE GEEAAKYDEP RHICEINFLC VHKQLREKRL APILIKEATR RVNRTNVWQA VYTAGVLLPT PYASGQYFHR SLNPEKLVEI RFSGIPAQYQ KFQNPMAMLK RNYQLPSAPK NSGLREMKPS DVPQVRRILM NYLDSFDVGP VFSDAEISHY LLPRDGVVFT YVVENVKKVT DFFSFYRIPS TVIGNSNYNL LNAAYVHYYA ATSIPLHQLI LDLLIVAHSR GFDVCNMVEI LDNRSFVEQL KFGPGDGHLR YYFYNWAYPK IKPSQVALVM L
NT Sequence
atgtctcgca atccatcgaa ctccgacgct gcgcatgcgt tctggagcac acagcccgta ccgcagacgg aagatgagac ggagaaaatt gtgttcgctg gtccgatgga cgagccaaag acggtagccg atattcctga ggagccgtac ccgatcgcca gcacatttga gtggtggacg ccgaacatgg aggcggccga cgacattcac gcaatttacg agcttcttcg ggataactac gtcgaagacg acgacagcat gtttcgtttc aactactccg aggagtttct tcagtgggca ctatgcccac cgaactacat cccggactgg cacgttgcag ttcgccgaaa ggcggataag aagctgctgg ccttcattgc cggcgttccc gtgacgttgc gcatgggcac tcccaagtac atgaaggtga aagcacagga aaagggcgaa ggggaggagg cggccaagta tgatgaaccc cgtcacatct gcgaaatcaa ctttctctgt gtccacaagc aactccggga gaagcggctt gccccgattt tgatcaaaga ggcgacgcgc cgcgtgaacc gcaccaacgt gtggcaggcg gtgtacacgg ctggggtgct gctacccact ccgtatgcat cagggcagta cttccaccgc agcctgaacc ccgagaagct tgtggagatc cgcttcagcg gcattccagc acagtaccaa aagtttcaga acccaatggc gatgctgaag cgcaactacc agctgccaag cgcgccgaag aactctggtc ttcgtgaaat gaagccgtct gacgttccgc aggtgcggcg gattctcatg aactacctgg acagcttcga tgtaggtccc gtctttagcg atgccgagat cagccactac ctccttccac gcgacggtgt ggtcttcacc tacgtggtgg aaaacgtcaa gaaggtgacc gacttctttt ccttctatcg aattccgtcg actgtgattg ggaacagcaa ttacaacctt ctgaacgcag cttacgttca ctactatgcc gcgacgagta tacctttgca tcaactcatt ctcgaccttt tgatcgtggc gcattcacgc ggcttcgacg tgtgcaatat ggtagagatc ctcgacaacc ggtctttcgt tgagcagctc aagtttggcc ccggcgacgg tcatcttcga tattacttct acaactgggc gtatccaaag atcaagcctt ctcaggttgc cttggtgatg ctgtag
Details for LemaA.18219.a.B2.GE42451
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS NSDAAHAFWS TQPVPQTEDE TEKIVFAGPM DEPKTVADIP EEPYPIASTF EWWTPNMEAA DDIHAIYELL RDNYVEDDDS MFRFNYSEEF LQWALCPPNY IPDWHVAVRR KADKKLLAFI AGVPVTLRMG TPKYMKVKAQ ERGEGEEAAK YDEPRHICEI NFLCVHKQLR EKRLAPILIK EATRRVNRTN VWQAVYTAGV LLPTPYASGQ YFHRSLNPEK LVEIRFSGIP AQYQKFQNPM AMLKRNYQLP SAPKNSGLRE MKPSDVPQVR RILMNYLDSF DVGPVFSDAE ISHYLLPRDG VVFTYVVEND KKVTDFFSFY RIPSTVIGNS NYNLLNAAYV HYYAATSIPL HQLILDLLIV AHSRGFDVCN MVEILDNRSF VEQLKFGAGD GHLRYYFYNW AYPKIK
Validated NT Sequence
aggcttgatc tttggatacg cccagttgta gaagtaatat cgaagatgac cgtcgccggc gccaaacttg agctgctcaa cgaaagaccg gttgtcgagg atctctacca tattgcacac gtcgaagccg cgtgaatgcg ccacgatcaa aaggtcgaga atgagttgat gcaaaggtat actcgtcgcg gcatagtagt gaacgtaagc tgcgttcaga aggttgtaat tgctgttccc aatcacagtc gacggaattc gatagaagga aaagaagtcg gtcaccttct tgtcgttttc caccacgtag gtgaagacca caccgtcgcg tggaaggagg tagtggctga tctcggcatc gctaaagacg ggacctacat cgaagctgtc caggtagttc atgagaatcc gccgcacctg cggaacgtca gacggcttca tttcacgaag accagagttc ttcggcgcgc ttggcagctg gtagttgcgc ttcagcatcg ccattgggtt ctgaaacttt tggtactgtg ctggaatgcc gctgaagcgg atctccacaa gcttctcggg gttcaggctg cggtggaagt actgccctga tgcatacgga gtgggtagca gcaccccagc cgtgtacacc gcctgccaca cgttggtgcg gttcacgcgg cgcgtcgcct ctttgatcaa aatcggggca agccgcttct cccggagttg cttgtggaca cagagaaagt tgatttcgca gatgtgacgg ggttcatcat acttggccgc ctcctcccct tcgccccttt cctgtgcttt caccttcatg tacttgggag tgcccatgcg caacgtcacg ggaacgccgg caatgaaggc cagcagcttc ttatccgcct ttcggcgaac tgcaacgtgc cagtccggga tgtagttcgg tgggcatagt gcccactgaa gaaactcctc ggagtagttg aaacgaaaca tgctgtcgtc gtcttcgacg tagttatccc gaagaagctc gtaaattgcg tgaatgtcgt cggccgcctc catgttcggc gtccaccact caaatgtgct ggcgatcggg tacggctcct caggaatatc ggctaccgtc tttggctcgt ccatcggacc agcgaacaca attttctccg tctcatcttc cgtctgcggt acgggctgtg tgctccagaa cgcatgcgca gcgtcggagt tcgacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHSN SDAAHAFWST QPVPQTEDET EKIVFAGPMD EPKTVADIPE EPYPIASTFE WWTPNMEAAD DIHAIYELLR DNYVEDDDSM FRFNYSEEFL QWALCPPNYI PDWHVAVRRK ADKKLLAFIA GVPVTLRMGT PKYMKVKAQE KGEGEEAAKY DEPRHICEIN FLCVHKQLRE KRLAPILIKE ATRRVNRTNV WQAVYTAGVL LPTPYASGQY FHRSLNPEKL VEIRFSGIPA QYQKFQNPMA MLKRNYQLPS APKNSGLREM KPSDVPQVRR ILMNYLDSFD VGPVFSDAEI SHYLLPRDGV VFTYVVENVK KVTDFFSFYR IPSTVIGNSN YNLLNAAYVH YYAATSIPLH QLILDLLIVA HSRGFDVCNM VEILDNRSFV EQLKFGPGDG HLRYYFYNWA YPKIKPSQVA LVML
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgaact ccgacgctgc gcatgcgttc tggagcacac agcccgtacc gcagacggaa gatgagacgg agaaaattgt gttcgctggt ccgatggacg agccaaagac ggtagccgat attcctgagg agccgtaccc gatcgccagc acatttgagt ggtggacgcc gaacatggag gcggccgacg acattcacgc aatttacgag cttcttcggg ataactacgt cgaagacgac gacagcatgt ttcgtttcaa ctactccgag gagtttcttc agtgggcact atgcccaccg aactacatcc cggactggca cgttgcagtt cgccgaaagg cggataagaa gctgctggcc ttcattgccg gcgttcccgt gacgttgcgc atgggcactc ccaagtacat gaaggtgaaa gcacaggaaa agggcgaagg ggaggaggcg gccaagtatg atgaaccccg tcacatctgc gaaatcaact ttctctgtgt ccacaagcaa ctccgggaga agcggcttgc cccgattttg atcaaagagg cgacgcgccg cgtgaaccgc accaacgtgt ggcaggcggt gtacacggct ggggtgctgc tacccactcc gtatgcatca gggcagtact tccaccgcag cctgaacccc gagaagcttg tggagatccg cttcagcggc attccagcac agtaccaaaa gtttcagaac ccaatggcga tgctgaagcg caactaccag ctgccaagcg cgccgaagaa ctctggtctt cgtgaaatga agccgtctga cgttccgcag gtgcggcgga ttctcatgaa ctacctggac agcttcgatg taggtcccgt ctttagcgat gccgagatca gccactacct ccttccacgc gacggtgtgg tcttcaccta cgtggtggaa aacgtcaaga aggtgaccga cttcttttcc ttctatcgaa ttccgtcgac tgtgattggg aacagcaatt acaaccttct gaacgcagct tacgttcact actatgccgc gacgagtata cctttgcatc aactcattct cgaccttttg atcgtggcgc attcacgcgg cttcgacgtg tgcaatatgg tagagatcct cgacaaccgg tctttcgttg agcagctcaa gtttggcccc ggcgacggtc atcttcgata ttacttctac aactgggcgt atccaaagat caagccttct caggttgcct tggtgatgct gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat