LemaA.18205.a

similar to histone methyltransferases; putative uncharacterized protein

CENTER ID: LemaA.18205.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18205.a.A1.GE34025 full length 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.18205.a.A1.PS01557 full length 1 299

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LmjF.07.0025
RefSeq: XP_001680906.1
UniProt: Q4QIU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVVRARKAR VDHGSKRSIS RFLVSEAAIG KLVKDISAGT DAAHPVTPRE LGSGSPHDPI HLPLRRTPNG SGCYHCTTEE CCCVEFEKIL SNTYARVPKK RMVEVSGARQ LCAKSLLTPF VTRLVRLMNI TEKDTFYDFG CGNGSVLFQV AFMTGAKCVG VEISEHNADV AREAWQLLRQ VLEKKYDRPM PRVEIITADL AELLSTPTYF DEEEGQTAIL ISNLLFPKPL THFLSERLRS APVGTRILCF DDLYPHARSV ASYRDPGAFE LFEMKDYFWQ EMSVEWCSME GRFFIHTRK
NT Sequence
atgcccgttg ttcgagcaag gaaagcacgc gttgaccacg ggtcaaagcg atctatttct cgctttctgg tctccgaggc ggcaatcgga aaactggtga aggacatctc agcgggcacg gatgctgctc atccagtaac gccaagggaa ctggggagcg ggtcgccgca tgaccccatt caccttccac ttcgaaggac tccaaatggt agcggctgct atcactgcac gacagaggag tgttgctgtg tagagtttga aaagatctta tcgaacacct acgctagagt gccgaagaag cgtatggtgg aggtgtccgg tgcgagacag ctttgtgcaa agtcgcttct gacacctttt gtcacaaggc tggtgcgtct gatgaatata acggagaaag acactttcta cgatttcgga tgcggaaatg gctcagtctt atttcaagtc gcctttatga cgggtgcgaa gtgtgttggt gtggaaatca gcgagcacaa cgcagatgtt gcacgagaag cctggcagct gctgcggcag gtgctggaga aaaagtacga tcggccgatg ccgagggtgg aaatcatcac ggcggatttg gctgaactcc tctctacgcc tacgtacttc gacgaggaag aaggacaaac ggctatcctg atatcgaatc ttctcttccc gaagcccctg acgcactttt tgtcagagcg tctccgatcg gcccctgtag gaactcgtat cctatgtttt gacgatttgt acccacacgc tcgctcagtg gcttcgtaca gagaccctgg tgcttttgag ctgttcgaaa tgaaagatta cttctggcaa gagatgagtg tggagtggtg ctcaatggaa ggcaggttct tcatacatac gcggaagtag
Details for LemaA.18205.a.A1.GE34025
HARVESTED ON: 6/4/2012
SEQUENCED ON: 6/5/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPVVRARKA RVDHGSKRSI SRFLVSEAAI GKLVKDISAG TDAAHPVTPR ELGSGSPHDP IHLPLRRTPN GSGCYHCTTE ECCCVEFEKI LSNTYARVPK KRMVEVSGAR QLCAKSLLTP FVTRLVRLMN ITEKDTFYDF GCGNGSVLFQ VAFMTGAKCV GVEISEHNAD VAREAWQLLR QVLEKKYDRP MPRVEIITAD LAELLSTPTY FDEEEGQTAI LISNLLFPKP LTHFLSERLR SAPVGTRILC FDDLYPHARS VASYRDPGAF ELFEMKDYFW QEMSVEWCSM EGRFFIHTRK
Validated NT Sequence
tacttccgcg tatgtatgaa gaacctgcct tccattgagc accactccac actcatctct tgccagaagt aatctttcat ttcgaacagc tcaaaagcac cagggtctct gtacgaagcc actgagcgag cgtgtgggta caaatcgtca aaacatagga tacgagttcc tacaggggcc gatcggagac gctctgacaa aaagtgcgtc aggggcttcg ggaagagaag attcgatatc aggatagccg tttgtccttc ttcctcgtcg aagtacgtag gcgtagagag gagttcagcc aaatccgccg tgatgatttc caccctcggc atcggccgat cgtacttttt ctccagcacc tgccgcagca gctgccaggc ttctcgtgca acatctgcgt tgtgctcgct gatttccaca ccaacacact tcgcacccgt cataaaggcg acttgaaata agactgagcc atttccgcat ccgaaatcgt agaaagtgtc tttctccgtt atattcatca gacgcaccag ccttgtgaca aaaggtgtca gaagcgactt tgcacaaagc tgtctcgcac cggacacctc caccatacgc ttcttcggca ctctagcgta ggtgttcgat aagatctttt caaactctac acagcaacac tcctctgtcg tgcagtgata gcagccgcta ccatttggag tccttcgaag tggaaggtga atggggtcat gcggcgaccc gctccccagt tcccttggcg ttactggatg agcagcatcc gtgcccgctg agatgtcctt caccagtttt ccgattgccg cctcggagac cagaaagcga gaaatagatc gctttgaccc gtggtcaacg cgtgctttcc ttgctcgaac aacgggcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atggtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPVVRARKA RVDHGSKRSI SRFLVSEAAI GKLVKDISAG TDAAHPVTPR ELGSGSPHDP IHLPLRRTPN GSGCYHCTTE ECCCVEFEKI LSNTYARVPK KRMVEVSGAR QLCAKSLLTP FVTRLVRLMN ITEKDTFYDF GCGNGSVLFQ VAFMTGAKCV GVEISEHNAD VAREAWQLLR QVLEKKYDRP MPRVEIITAD LAELLSTPTY FDEEEGQTAI LISNLLFPKP LTHFLSERLR SAPVGTRILC FDDLYPHARS VASYRDPGAF ELFEMKDYFW QEMSVEWCSM EGRFFIHTRK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgttgtt cgagcaagga aagcacgcgt tgaccacggg tcaaagcgat ctatttctcg ctttctggtc tccgaggcgg caatcggaaa actggtgaag gacatctcag cgggcacgga tgctgctcat ccagtaacgc caagggaact ggggagcggg tcgccgcatg accccattca ccttccactt cgaaggactc caaatggtag cggctgctat cactgcacga cagaggagtg ttgctgtgta gagtttgaaa agatcttatc gaacacctac gctagagtgc cgaagaagcg tatggtggag gtgtccggtg cgagacagct ttgtgcaaag tcgcttctga caccttttgt cacaaggctg gtgcgtctga tgaatataac ggagaaagac actttctacg atttcggatg cggaaatggc tcagtcttat ttcaagtcgc ctttatgacg ggtgcgaagt gtgttggtgt ggaaatcagc gagcacaacg cagatgttgc acgagaagcc tggcagctgc tgcggcaggt gctggagaaa aagtacgatc ggccgatgcc gagggtggaa atcatcacgg cggatttggc tgaactcctc tctacgccta cgtacttcga cgaggaagaa ggacaaacgg ctatcctgat atcgaatctt ctcttcccga agcccctgac gcactttttg tcagagcgtc tccgatcggc ccctgtagga actcgtatcc tatgttttga cgatttgtac ccacacgctc gctcagtggc ttcgtacaga gaccctggtg cttttgagct gttcgaaatg aaagattact tctggcaaga gatgagtgtg gagtggtgct caatggaagg caggttcttc atacatacgc ggaagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for LemaA.18205.a.A1.PS01557
PURIFICATION DATe: 7/11/2012
CONCENTRATION: 42.6mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPVVRARKA RVDHGSKRSI SRFLVSEAAI GKLVKDISAG TDAAHPVTPR ELGSGSPHDP IHLPLRRTPN GSGCYHCTTE ECCCVEFEKI LSNTYARVPK KRMVEVSGAR QLCAKSLLTP FVTRLVRLMN ITEKDTFYDF GCGNGSVLFQ VAFMTGAKCV GVEISEHNAD VAREAWQLLR QVLEKKYDRP MPRVEIITAD LAELLSTPTY FDEEEGQTAI LISNLLFPKP LTHFLSERLR SAPVGTRILC FDDLYPHARS VASYRDPGAF ELFEMKDYFW QEMSVEWCSM EGRFFIHTRK
Validated NT Sequence
tacttccgcg tatgtatgaa gaacctgcct tccattgagc accactccac actcatctct tgccagaagt aatctttcat ttcgaacagc tcaaaagcac cagggtctct gtacgaagcc actgagcgag cgtgtgggta caaatcgtca aaacatagga tacgagttcc tacaggggcc gatcggagac gctctgacaa aaagtgcgtc aggggcttcg ggaagagaag attcgatatc aggatagccg tttgtccttc ttcctcgtcg aagtacgtag gcgtagagag gagttcagcc aaatccgccg tgatgatttc caccctcggc atcggccgat cgtacttttt ctccagcacc tgccgcagca gctgccaggc ttctcgtgca acatctgcgt tgtgctcgct gatttccaca ccaacacact tcgcacccgt cataaaggcg acttgaaata agactgagcc atttccgcat ccgaaatcgt agaaagtgtc tttctccgtt atattcatca gacgcaccag ccttgtgaca aaaggtgtca gaagcgactt tgcacaaagc tgtctcgcac cggacacctc caccatacgc ttcttcggca ctctagcgta ggtgttcgat aagatctttt caaactctac acagcaacac tcctctgtcg tgcagtgata gcagccgcta ccatttggag tccttcgaag tggaaggtga atggggtcat gcggcgaccc gctccccagt tcccttggcg ttactggatg agcagcatcc gtgcccgctg agatgtcctt caccagtttt ccgattgccg cctcggagac cagaaagcga gaaatagatc gctttgaccc gtggtcaacg cgtgctttcc ttgctcgaac aacgggcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atggtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPVVRARKA RVDHGSKRSI SRFLVSEAAI GKLVKDISAG TDAAHPVTPR ELGSGSPHDP IHLPLRRTPN GSGCYHCTTE ECCCVEFEKI LSNTYARVPK KRMVEVSGAR QLCAKSLLTP FVTRLVRLMN ITEKDTFYDF GCGNGSVLFQ VAFMTGAKCV GVEISEHNAD VAREAWQLLR QVLEKKYDRP MPRVEIITAD LAELLSTPTY FDEEEGQTAI LISNLLFPKP LTHFLSERLR SAPVGTRILC FDDLYPHARS VASYRDPGAF ELFEMKDYFW QEMSVEWCSM EGRFFIHTRK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGTTGTT CGAGCAAGGA AAGCACGCGT TGACCACGGG TCAAAGCGAT CTATTTCTCG CTTTCTGGTC TCCGAGGCGG CAATCGGAAA ACTGGTGAAG GACATCTCAG CGGGCACGGA TGCTGCTCAT CCAGTAACGC CAAGGGAACT GGGGAGCGGG TCGCCGCATG ACCCCATTCA CCTTCCACTT CGAAGGACTC CAAATGGTAG CGGCTGCTAT CACTGCACGA CAGAGGAGTG TTGCTGTGTA GAGTTTGAAA AGATCTTATC GAACACCTAC GCTAGAGTGC CGAAGAAGCG TATGGTGGAG GTGTCCGGTG CGAGACAGCT TTGTGCAAAG TCGCTTCTGA CACCTTTTGT CACAAGGCTG GTGCGTCTGA TGAATATAAC GGAGAAAGAC ACTTTCTACG ATTTCGGATG CGGAAATGGC TCAGTCTTAT TTCAAGTCGC CTTTATGACG GGTGCGAAGT GTGTTGGTGT GGAAATCAGC GAGCACAACG CAGATGTTGC ACGAGAAGCC TGGCAGCTGC TGCGGCAGGT GCTGGAGAAA AAGTACGATC GGCCGATGCC GAGGGTGGAA ATCATCACGG CGGATTTGGC TGAACTCCTC TCTACGCCTA CGTACTTCGA CGAGGAAGAA GGACAAACGG CTATCCTGAT ATCGAATCTT CTCTTCCCGA AGCCCCTGAC GCACTTTTTG TCAGAGCGTC TCCGATCGGC CCCTGTAGGA ACTCGTATCC TATGTTTTGA CGATTTGTAC CCACACGCTC GCTCAGTGGC TTCGTACAGA GACCCTGGTG CTTTTGAGCT GTTCGAAATG AAAGATTACT TCTGGCAAGA GATGAGTGTG GAGTGGTGCT CAATGGAAGG CAGGTTCTTC ATACATACGC GGAAGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA