LemaA.17936.a

fructose-1,6-bisphosphatase, cytosolic, putative

CENTER ID: LemaA.17936.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LemaA.17936.a.B1.GE34433 full length 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LemaA.17936.a.B1.PW36258 full length 1 351

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LmjF04.1160

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDVRRTPTPT TLTQYIIKSQ PPHSRGDFTL LMMAIQTSVK VIEKNIRRAG MKGMLGYIAG QSANATGDHQ AKLDVISNIA FKAYLLSSTS VCVLGSEEEE QMIIAESGRR GDYLIFFDPL DGSSNIDANV SVGSIWGVWR LPKDTTINSV EDANAVIRML KGTDMVSAGY AVYGSATNLV LTSGHGVDGF TLDPNIGEFI LTHPHISIPK KRSIYSVNEG NYGKWEPWFK EYIDYLKMNK TTRYSARYIG SMVGDIHRTL LYGGIFCYPK DANQVEGKLR LLYEAAPMAM IVEQAGGKAV GSNGRILEQS ITRLHQRTPV YFGSRQEVDL CMAFRDRNVK TEALAPTSSK L
NT Sequence
atggacgtca gacgcacccc cactcccact actctcaccc agtacatcat caagagccaa ccgccgcaca gtcgtggcga cttcacgctc ttgatgatgg cgattcagac gtcggtgaag gtgattgaga agaacatccg acgtgctggc atgaagggca tgctgggcta cattgctggg cagtcggcga acgcgacggg cgaccatcag gcgaagctgg atgtgatctc gaacatagcg ttcaaggcgt acctgctgag ctccaccagc gtgtgcgtgc tcggcagcga ggaggaagag cagatgatca tcgccgagag cggccgccgt ggcgactacc tcatcttctt cgaccctctt gacggcagca gcaacattga tgccaacgtc tccgtaggct ctatctgggg cgtgtggcgg ctgcccaagg acaccaccat aaacagcgtc gaagacgcca acgccgtgat ccgcatgctt aagggaacag acatggtttc tgccgggtac gccgtgtacg gcagtgccac gaacctcgtg ctgacgagcg gacacggcgt ggacggcttc acactggacc ccaacattgg cgagttcatc ctgacacacc cgcacatcag catcccgaaa aagcggagca tctacagcgt caacgagggc aattacggca agtgggagcc gtggtttaag gagtatatcg actacctcaa gatgaacaag acaacccgct acagcgcgcg ttacatcggc tccatggtcg gcgacatcca ccgcacgctc ctctacggcg gtatattctg ttaccccaag gacgcaaacc aggtggaggg gaagctgcgc cttctgtacg aggccgcacc gatggcgatg atcgtggagc aggcaggcgg gaaggcggtg ggcagcaacg gccgtatcct ggagcagtcc atcactcgcc ttcatcagcg cacgccggtc tacttcggca gccgccagga ggtggacctg tgcatggcct tccgcgaccg caacgtgaag acggaggcac tagcaccgac ttccagcaag ctctaa
Details for LemaA.17936.a.B1.GE34433
HARVESTED ON: 2/6/2012
SEQUENCED ON: 2/21/2012
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Validated AA Sequence
MMAIQTSVKV IEKNIRRAGM KGMLGYIAGQ SANATGDHQA KLDVISNIAF KAYLLSSTSV CVLGSEEEEQ MIIAESGRRG DYLIFFDPLD GSSNIDANVS VGSIWGVWRL PKDTTINSVE DANAVIRMLK GTDMVSAGYA VYGSATNLVL TSGHGVDGFT LDPNIGEFIL THPHISIPKK RSIYSVNEGN YGKWEPWFKE YIDYLKMNKT TRYSARYIGS MVGDIHRTLL YGGIFCYPKD ANQVEGKLRL LYEAAPMAMI VEQAGGKAVG SNGRILEQSI TRLHQRTPVY FGSRQEVDLC MAFRDRNVKT EALAPT
Validated NT Sequence
gaagtcggtg ctagtgcctc cgtcttcacg ttgcggtcgc ggaaggccat gcacaggtcc acctcctggc ggctgccgaa gtagaccggc gtgcgctgat gaaggcgagt gatggactgc tccaggatac ggccgttgct gcccaccgcc ttcccgcctg cctgctccac gatcatcgcc atcggtgcgg cctcgtacag aaggcgcagc ttcccctcca cctggtttgc gtccttgggg taacagaata taccgccgta gaggagcgtg cggtggatgt cgccgaccat ggagccgatg taacgcgcgc tgtagcgggt tgtcttgttc atcttgaggt agtcgatata ctccttaaac cacggctccc acttgccgta attgccctcg ttgacgctgt agatgctccg ctttttcggg atgctgatgt gcgggtgtgt caggatgaac tcgccaatgt tggggtccag tgtgaagccg tccacgccgt gtccgctcgt cagcacgagg ttcgtggcac tgccgtacac ggcgtacccg gcagaaacca tgtctgttcc cttaagcatg cggatcacgg cgttggcgtc ttcgacgctg tttatggtgg tgtccttggg cagccgccac acgccccaga tagagcctac ggagacgttg gcatcaatgt tgctgctgcc gtcaagaggg tcgaagaaga tgaggtagtc gccacggcgg ccgctctcgg cgatgatcat ctgctcttcc tcctcgctgc cgagcacgca cacgctggtg gagctcagca ggtacgcctt gaacgctatg ttcgagatca catccagctt cgcctgatgg tcgcccgtcg cgttcgccga ctgcccagca atgtagccca gcatgccctt catgccagca cgtcggatgt tcttctcaat caccttcacc gacgtctgaa tcgccatcat caagagcgtg aagtcgccac gactgtgcgg cggttggctc ttgatgatgt actgggtgag agtagtggga gtgggggtgc gtctgacgtc
Expected Protein Sequence
MAHHHHHHMD VRRTPTPTTL TQYIIKSQPP HSRGDFTLLM MAIQTSVKVI EKNIRRAGMK GMLGYIAGQS ANATGDHQAK LDVISNIAFK AYLLSSTSVC VLGSEEEEQM IIAESGRRGD YLIFFDPLDG SSNIDANVSV GSIWGVWRLP KDTTINSVED ANAVIRMLKG TDMVSAGYAV YGSATNLVLT SGHGVDGFTL DPNIGEFILT HPHISIPKKR SIYSVNEGNY GKWEPWFKEY IDYLKMNKTT RYSARYIGSM VGDIHRTLLY GGIFCYPKDA NQVEGKLRLL YEAAPMAMIV EQAGGKAVGS NGRILEQSIT RLHQRTPVYF GSRQEVDLCM AFRDRNVKTE ALAPTSSKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg tcagacgcac ccccactccc actactctca cccagtacat catcaagagc caaccgccgc acagtcgtgg cgacttcacg ctcttgatga tggcgattca gacgtcggtg aaggtgattg agaagaacat ccgacgtgct ggcatgaagg gcatgctggg ctacattgct gggcagtcgg cgaacgcgac gggcgaccat caggcgaagc tggatgtgat ctcgaacata gcgttcaagg cgtacctgct gagctccacc agcgtgtgcg tgctcggcag cgaggaggaa gagcagatga tcatcgccga gagcggccgc cgtggcgact acctcatctt cttcgaccct cttgacggca gcagcaacat tgatgccaac gtctccgtag gctctatctg gggcgtgtgg cggctgccca aggacaccac cataaacagc gtcgaagacg ccaacgccgt gatccgcatg cttaagggaa cagacatggt ttctgccggg tacgccgtgt acggcagtgc cacgaacctc gtgctgacga gcggacacgg cgtggacggc ttcacactgg accccaacat tggcgagttc atcctgacac acccgcacat cagcatcccg aaaaagcgga gcatctacag cgtcaacgag ggcaattacg gcaagtggga gccgtggttt aaggagtata tcgactacct caagatgaac aagacaaccc gctacagcgc gcgttacatc ggctccatgg tcggcgacat ccaccgcacg ctcctctacg gcggtatatt ctgttacccc aaggacgcaa accaggtgga ggggaagctg cgccttctgt acgaggccgc accgatggcg atgatcgtgg agcaggcagg cgggaaggcg gtgggcagca acggccgtat cctggagcag tccatcactc gccttcatca gcgcacgccg gtctacttcg gcagccgcca ggaggtggac ctgtgcatgg ccttccgcga ccgcaacgtg aagacggagg cactagcacc gacttccagc aagctctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for LemaA.17936.a.B1.PW36258
PURIFICATION DATe: 5/17/2012
CONCENTRATION: 6.9mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 88
Protocol Notes
notes unavailable
Validated AA Sequence
MMAIQTSVKV IEKNIRRAGM KGMLGYIAGQ SANATGDHQA KLDVISNIAF KAYLLSSTSV CVLGSEEEEQ MIIAESGRRG DYLIFFDPLD GSSNIDANVS VGSIWGVWRL PKDTTINSVE DANAVIRMLK GTDMVSAGYA VYGSATNLVL TSGHGVDGFT LDPNIGEFIL THPHISIPKK RSIYSVNEGN YGKWEPWFKE YIDYLKMNKT TRYSARYIGS MVGDIHRTLL YGGIFCYPKD ANQVEGKLRL LYEAAPMAMI VEQAGGKAVG SNGRILEQSI TRLHQRTPVY FGSRQEVDLC MAFRDRNVKT EALAPT
Validated NT Sequence
gaagtcggtg ctagtgcctc cgtcttcacg ttgcggtcgc ggaaggccat gcacaggtcc acctcctggc ggctgccgaa gtagaccggc gtgcgctgat gaaggcgagt gatggactgc tccaggatac ggccgttgct gcccaccgcc ttcccgcctg cctgctccac gatcatcgcc atcggtgcgg cctcgtacag aaggcgcagc ttcccctcca cctggtttgc gtccttgggg taacagaata taccgccgta gaggagcgtg cggtggatgt cgccgaccat ggagccgatg taacgcgcgc tgtagcgggt tgtcttgttc atcttgaggt agtcgatata ctccttaaac cacggctccc acttgccgta attgccctcg ttgacgctgt agatgctccg ctttttcggg atgctgatgt gcgggtgtgt caggatgaac tcgccaatgt tggggtccag tgtgaagccg tccacgccgt gtccgctcgt cagcacgagg ttcgtggcac tgccgtacac ggcgtacccg gcagaaacca tgtctgttcc cttaagcatg cggatcacgg cgttggcgtc ttcgacgctg tttatggtgg tgtccttggg cagccgccac acgccccaga tagagcctac ggagacgttg gcatcaatgt tgctgctgcc gtcaagaggg tcgaagaaga tgaggtagtc gccacggcgg ccgctctcgg cgatgatcat ctgctcttcc tcctcgctgc cgagcacgca cacgctggtg gagctcagca ggtacgcctt gaacgctatg ttcgagatca catccagctt cgcctgatgg tcgcccgtcg cgttcgccga ctgcccagca atgtagccca gcatgccctt catgccagca cgtcggatgt tcttctcaat caccttcacc gacgtctgaa tcgccatcat caagagcgtg aagtcgccac gactgtgcgg cggttggctc ttgatgatgt actgggtgag agtagtggga gtgggggtgc gtctgacgtc
Expressed Protein Sequence
MAHHHHHHMD VRRTPTPTTL TQYIIKSQPP HSRGDFTLLM MAIQTSVKVI EKNIRRAGMK GMLGYIAGQS ANATGDHQAK LDVISNIAFK AYLLSSTSVC VLGSEEEEQM IIAESGRRGD YLIFFDPLDG SSNIDANVSV GSIWGVWRLP KDTTINSVED ANAVIRMLKG TDMVSAGYAV YGSATNLVLT SGHGVDGFTL DPNIGEFILT HPHISIPKKR SIYSVNEGNY GKWEPWFKEY IDYLKMNKTT RYSARYIGSM VGDIHRTLLY GGIFCYPKDA NQVEGKLRLL YEAAPMAMIV EQAGGKAVGS NGRILEQSIT RLHQRTPVYF GSRQEVDLCM AFRDRNVKTE ALAPTSSKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACG TCAGACGCAC CCCCACTCCC ACTACTCTCA CCCAGTACAT CATCAAGAGC CAACCGCCGC ACAGTCGTGG CGACTTCACG CTCTTGATGA TGGCGATTCA GACGTCGGTG AAGGTGATTG AGAAGAACAT CCGACGTGCT GGCATGAAGG GCATGCTGGG CTACATTGCT GGGCAGTCGG CGAACGCGAC GGGCGACCAT CAGGCGAAGC TGGATGTGAT CTCGAACATA GCGTTCAAGG CGTACCTGCT GAGCTCCACC AGCGTGTGCG TGCTCGGCAG CGAGGAGGAA GAGCAGATGA TCATCGCCGA GAGCGGCCGC CGTGGCGACT ACCTCATCTT CTTCGACCCT CTTGACGGCA GCAGCAACAT TGATGCCAAC GTCTCCGTAG GCTCTATCTG GGGCGTGTGG CGGCTGCCCA AGGACACCAC CATAAACAGC GTCGAAGACG CCAACGCCGT GATCCGCATG CTTAAGGGAA CAGACATGGT TTCTGCCGGG TACGCCGTGT ACGGCAGTGC CACGAACCTC GTGCTGACGA GCGGACACGG CGTGGACGGC TTCACACTGG ACCCCAACAT TGGCGAGTTC ATCCTGACAC ACCCGCACAT CAGCATCCCG AAAAAGCGGA GCATCTACAG CGTCAACGAG GGCAATTACG GCAAGTGGGA GCCGTGGTTT AAGGAGTATA TCGACTACCT CAAGATGAAC AAGACAACCC GCTACAGCGC GCGTTACATC GGCTCCATGG TCGGCGACAT CCACCGCACG CTCCTCTACG GCGGTATATT CTGTTACCCC AAGGACGCAA ACCAGGTGGA GGGGAAGCTG CGCCTTCTGT ACGAGGCCGC ACCGATGGCG ATGATCGTGG AGCAGGCAGG CGGGAAGGCG GTGGGCAGCA ACGGCCGTAT CCTGGAGCAG TCCATCACTC GCCTTCATCA GCGCACGCCG GTCTACTTCG GCAGCCGCCA GGAGGTGGAC CTGTGCATGG CCTTCCGCGA CCGCAACGTG AAGACGGAGG CACTAGCACC GACTTCCAGC AAGCTCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T