SSGCID: Target Page

LemaA.10384.b

cell division protein kinase 2

CENTER ID:	LemaA.10384.b
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.10384.b.B1.GE34426	full length		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.10384.b.B1.PS01454	full length		1	301

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF21.1080
RefSeq:	XP_001683060.1
UniProt:	Q4QC88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSRYERQEK IGEGTYGVVY KARDTSTSAT VALKRIRLDS EEEGVPCTAI REISLLKELR HENIVKLLDV CHSEHRLTIV FEYLDLDLKK YLDRENGNLD AATIQHFMRD LLRGVAFCHQ RSVLHRDLKP QNLLISREKE LKLGDFGLGR SFAIPVRKFT NEVVTLWYRP PDVLLGSMQY GPPVDVWSVG CIFSEMATGT PLFAGKNDAD QLMRIFRFLG TPNNRVWPSM NQYPNSNNML SQPEFLQNFE PEWSNVLGSV PGYEKLGCAG VDLLEKLLRY EPSERITAAD ALNHPYFSLQ F

NT Sequence

atgaccagcc ggtacgagcg gcaggagaag atcggcgagg gcacctacgg cgtggtgtac aaggcccgag acacgtccac ttccgcgacg gtcgcgttga agcgcattcg gcttgactcg gaggaggagg gcgtgccgtg taccgccatt cgcgagattt cgctgctgaa ggagctgcga catgagaaca tcgtgaagct gctggacgtg tgccacagcg agcaccgtct gacgatcgtc ttcgagtatt tagacctaga cttgaagaag tatctcgacc gcgagaacgg caacctcgat gcggcgacaa ttcagcactt tatgcgcgac ctgctgcgcg gcgtcgcctt ctgccaccag cgcagtgtcc tgcatcgcga cctgaagccg cagaatcttc tcatctcgcg cgagaaagag ctgaagctag gcgacttcgg tctcggtcgt tccttcgcca tcccagtgcg caaattcacg aacgaggtgg tgacgctgtg gtaccgaccg cccgacgttc tgctcggctc gatgcagtac ggtccgccgg tggacgtgtg gtctgtgggg tgcatcttct ccgagatggc caccgggacg ccgctcttcg ccgggaagaa cgatgccgac cagctcatgc ggatcttccg cttcctaggc acaccgaaca accgggtttg gccctccatg aaccagtacc cgaactcgaa caacatgctg tcgcagccgg agtttttgca gaacttcgag ccggaatgga gcaacgtgct cggctccgtg cctgggtacg agaagcttgg ttgtgctggt gtcgatctgc tagaaaagtt gctacgctac gagccgtcgg agcgcattac ggcggcagac gcgttgaatc acccgtactt tagcctccag ttttag

Details for LemaA.10384.b.B1.GE34426

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MTSRYERQEK IGEGTYGVVY KARDTSTSAT VALKRIRLDS EEEGVPCTAI REISLLKELR HENIVKLLDV CHSEHRLTIV FEYLDLDLKK YLDRENGNLD AATIQHFMRD LLRGVAFCHQ RSVLHRDLKP QNLLISREKE LKLGDFGLGR SFAIPVRKFT NEVVTLWYRP PDVLLGSMQY GPPVDVWSVG CIFSEMATGT PLFAGKNDAD QLMRIFRFLG TPNNRVWPSM NQYPNSNNML SQPEFLQNFE PXWSNVLGSV PGYEKLGCAG VDLLEKLLRY EPSERITAAD ALNHPYFSLQ F

Validated NT Sequence

atcttactca cttaaaactg gaggctaaag tacgggtgat tcaacgcgtc tgccgccgta atgcgctccg acggctcgta gcgtagcaac ttttctagca gatcgacacc agcacaacca agcttctcgt acccaggcac ggagccgagc acgttgctcc atncnggctc gaagttctgc aaaaactccg gctgcgacag catgttgttc gagttcgggt actggttcat ggagggccaa acccggttgt tcggtgtgcc taggaagcgg aagatccgca tgagctggtc ggcatcgttc ttcccggcga agagcggcgt cccggtggcc atctcggaga agatgcaccc cacagaccac acgtccaccg gcggaccgta ctgcatcgag ccgagcagaa cgtcgggcgg tcggtaccac agcgtcacca cctcgttcgt gaatttgcgc actgggatgg cgaaggaacg accgagaccg aagtcgccta gcttcagctc tttctcgcgc gagatgagaa gattctgcgg cttcaggtcg cgatgcagga cactgcgctg gtggcagaag gcgacgccgc gcagcaggtc gcgcataaag tgctgaattg tcgccgcatc gaggttgccg ttctcgcggt cgagatactt cttcaagtct aggtctaaat actcgaagac gatcgtcaga cggtgctcgc tgtggcacac gtccagcagc ttcacgatgt tctcatgtcg cagctccttc agcagcgaaa tctcgcgaat ggcggtacac ggcacgccct cctcctccga gtcaagccga atgcgcttca acgcgaccgt cgcggaagtg gacgtgtctc gggccttgta caccacgccg taggtgccct cgccgatctt ctcctgccgc tcgtaccggc tggtcatatg gtggtggtgg

Expected Protein Sequence

MAHHHHHHMT SRYERQEKIG EGTYGVVYKA RDTSTSATVA LKRIRLDSEE EGVPCTAIRE ISLLKELRHE NIVKLLDVCH SEHRLTIVFE YLDLDLKKYL DRENGNLDAA TIQHFMRDLL RGVAFCHQRS VLHRDLKPQN LLISREKELK LGDFGLGRSF AIPVRKFTNE VVTLWYRPPD VLLGSMQYGP PVDVWSVGCI FSEMATGTPL FAGKNDADQL MRIFRFLGTP NNRVWPSMNQ YPNSNNMLSQ PEFLQNFEPE WSNVLGSVPG YEKLGCAGVD LLEKLLRYEP SERITAADAL NHPYFSLQF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca gccggtacga gcggcaggag aagatcggcg agggcaccta cggcgtggtg tacaaggccc gagacacgtc cacttccgcg acggtcgcgt tgaagcgcat tcggcttgac tcggaggagg agggcgtgcc gtgtaccgcc attcgcgaga tttcgctgct gaaggagctg cgacatgaga acatcgtgaa gctgctggac gtgtgccaca gcgagcaccg tctgacgatc gtcttcgagt atttagacct agacttgaag aagtatctcg accgcgagaa cggcaacctc gatgcggcga caattcagca ctttatgcgc gacctgctgc gcggcgtcgc cttctgccac cagcgcagtg tcctgcatcg cgacctgaag ccgcagaatc ttctcatctc gcgcgagaaa gagctgaagc taggcgactt cggtctcggt cgttccttcg ccatcccagt gcgcaaattc acgaacgagg tggtgacgct gtggtaccga ccgcccgacg ttctgctcgg ctcgatgcag tacggtccgc cggtggacgt gtggtctgtg gggtgcatct tctccgagat ggccaccggg acgccgctct tcgccgggaa gaacgatgcc gaccagctca tgcggatctt ccgcttccta ggcacaccga acaaccgggt ttggccctcc atgaaccagt acccgaactc gaacaacatg ctgtcgcagc cggagttttt gcagaacttc gagccggaat ggagcaacgt gctcggctcc gtgcctgggt acgagaagct tggttgtgct ggtgtcgatc tgctagaaaa gttgctacgc tacgagccgt cggagcgcat tacggcggca gacgcgttga atcacccgta ctttagcctc cagttttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LemaA.10384.b.B1.PS01454

PURIFICATION DATe:	4/10/2012
CONCENTRATION:	6.24mg/ml
OBSERVED MW:	32kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

MTSRYERQEK IGEGTYGVVY KARDTSTSAT VALKRIRLDS EEEGVPCTAI REISLLKELR HENIVKLLDV CHSEHRLTIV FEYLDLDLKK YLDRENGNLD AATIQHFMRD LLRGVAFCHQ RSVLHRDLKP QNLLISREKE LKLGDFGLGR SFAIPVRKFT NEVVTLWYRP PDVLLGSMQY GPPVDVWSVG CIFSEMATGT PLFAGKNDAD QLMRIFRFLG TPNNRVWPSM NQYPNSNNML SQPEFLQNFE PXWSNVLGSV PGYEKLGCAG VDLLEKLLRY EPSERITAAD ALNHPYFSLQ F

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCA GCCGGTACGA GCGGCAGGAG AAGATCGGCG AGGGCACCTA CGGCGTGGTG TACAAGGCCC GAGACACGTC CACTTCCGCG ACGGTCGCGT TGAAGCGCAT TCGGCTTGAC TCGGAGGAGG AGGGCGTGCC GTGTACCGCC ATTCGCGAGA TTTCGCTGCT GAAGGAGCTG CGACATGAGA ACATCGTGAA GCTGCTGGAC GTGTGCCACA GCGAGCACCG TCTGACGATC GTCTTCGAGT ATTTAGACCT AGACTTGAAG AAGTATCTCG ACCGCGAGAA CGGCAACCTC GATGCGGCGA CAATTCAGCA CTTTATGCGC GACCTGCTGC GCGGCGTCGC CTTCTGCCAC CAGCGCAGTG TCCTGCATCG CGACCTGAAG CCGCAGAATC TTCTCATCTC GCGCGAGAAA GAGCTGAAGC TAGGCGACTT CGGTCTCGGT CGTTCCTTCG CCATCCCAGT GCGCAAATTC ACGAACGAGG TGGTGACGCT GTGGTACCGA CCGCCCGACG TTCTGCTCGG CTCGATGCAG TACGGTCCGC CGGTGGACGT GTGGTCTGTG GGGTGCATCT TCTCCGAGAT GGCCACCGGG ACGCCGCTCT TCGCCGGGAA GAACGATGCC GACCAGCTCA TGCGGATCTT CCGCTTCCTA GGCACACCGA ACAACCGGGT TTGGCCCTCC ATGAACCAGT ACCCGAACTC GAACAACATG CTGTCGCAGC CGGAGTTTTT GCAGAACTTC GAGCCGGAAT GGAGCAACGT GCTCGGCTCC GTGCCTGGGT ACGAGAAGCT TGGTTGTGCT GGTGTCGATC TGCTAGAAAA GTTGCTACGC TACGAGCCGT CGGAGCGCAT TACGGCGGCA GACGCGTTGA ATCACCCGTA CTTTAGCCTC CAGTTTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T