SSGCID: Target Page

LemaA.10384.a

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID:	LemaA.10384.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.10384.a.B1.GE34437	full length		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF26.0040
RefSeq:	XP_001684011.1
UniProt:	Q4Q9I7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKYALGPVI GEGQFGSVRM ATVKATGQTV AVKLLHVPRL TEGIPHPVAR ELLIASRVDS PFLVKTIEIT PCGSHMAVVM ERCMEDLACV LRKCSPAHPL PLLLTQSYLR MLLTALHALH SSGILHRDVK PSNCFVSVDG CLKLGDFGLS RPLGSDMSHE VVSRWYRAPE LLFGQRRYGG EVDMWAAGCV FAELLRGHGR PFFTGDGDIS QIAKIFDVFG TPTGATCSFY RQLPDWEKVY FEEKKGTGLR VLLPFVPPEA LDLLTKMLAL DPASRCSAAE ALTHSFFALS DTLLRA

NT Sequence

atggacaagt acgcgttggg gccggttatc ggggagggac agtttgggag tgtgcgcatg gcgacagtca aggcgacggg gcagacggtt gccgtgaaac tgctacatgt gcctcggctt acggaaggca ttccgcatcc ggtggcgcgg gagcttctca ttgcctctcg tgtcgactcg ccgtttctgg tgaagaccat cgagattaca ccgtgcgggt cccacatggc tgtggtgatg gagcgctgta tggaggacct ggcgtgtgtc ttgcgcaagt gtagtccggc tcacccactt ccgctgctgt tgacgcagtc gtatctgcgc atgcttctca ccgcgctgca cgcattgcac tcgagcggta ttctgcaccg tgatgtgaaa ccgtcgaact gctttgtttc tgtggatggg tgcctgaagc tcggcgactt tggcctcagc cgcccactag gctcagacat gtcacacgag gttgtgtcgc gctggtaccg cgcgccggag ctgctctttg gccagcgacg ctacgggggc gaggtggaca tgtgggcggc tgggtgcgtc ttcgccgaac tcctgcgtgg tcacggccgc cctttcttta caggcgatgg ggatatcagc cagattgcga agatattcga cgtcttcggc acccccacgg gcgcaacatg tagtttctac cgtcagctgc ctgactggga gaaggtttat ttcgaagaga agaagggaac tggcctgcgt gtgctgctgc cctttgtgcc ccctgaggcg cttgacttgc tgacaaaaat gctagccctg gacccggcat ctcgctgcag cgccgcagag gcgctcaccc attcattttt cgctctctca gataccttgc tgcgtgcatg a

Details for LemaA.10384.a.B1.GE34437

HARVESTED ON:	2/6/2012
SEQUENCED ON:	10/23/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD KYALGPVIGE GQFGSVRMAT VKATGQTVAV KLLHVPRLTE GIPHPVAREL LIASRVDSPF LVKTIEITPC GSHMAVVMER CMEDLACVLR KCSPAHPLPL LLTQSYLRML LTALHALHSS GILHRDVKPS NCFVSVDGCL KLGDFGLSRP LGSDMSHEVV SRWYRAPELL FGQRRYGGEV DMWAAGCVFA ELLRGHGRPF FTGDGDISQI AKIFDVFGTP TGATCSFYRQ LPDWEKVYFE EKKGTGLRVL LPFVPPEALD LLTKMLALDP ASRCSAAEAL THSFFALSDT LLRA

Validated NT Sequence

atggctcacc accaccacca ccatatggac aagtacgcgt tggggccggt tatcggggag ggacagtttg ggagtgtgcg catggcgaca gtcaaggcga cggggcagac ggttgccgtg aaactgctac atgtgcctcg gcttacggaa ggcattccgc atccggtggc gcgggagctt ctcattgcct ctcgtgtcga ctcgccgttt ctggtgaaga ccatcgagat tacaccgtgc gggtcccaca tggctgtggt gatggagcgc tgtatggagg acctggcgtg tgtcttgcgc aagtgtagtc cggctcaccc acttccgctg ctgttgacgc agtcgtatct gcgcatgctt ctcaccgcgc tgcacgcatt gcactcgagc ggtattctgc accgtgatgt gaaaccgtcg aactgctttg tttctgtgga tgggtgcctg aagctcggcg actttggcct cagccgccca ctaggctcag acatgtcaca cgaggttgtg tcgcgctggt accgcgcgcc ggagctgctc tttggccagc gacgctacgg gggcgaggtg gacatgtggg cggctgggtg cgtcttcgcc gaactcctgc gtggtcacgg ccgccctttc tttacaggcg atggggatat cagccagatt gcgaagatat tcgacgtctt cggcaccccc acgggcgcaa catgtagttt ctaccgtcag ctgcctgact gggagaaggt ttatttcgaa gagaagaagg gaactggcct gcgtgtgctg ctgccctttg tgccccctga ggcgcttgac ttgctgacaa aaatgctagc cctggacccg gcatctcgct gcagcgccgc agaggcgctc acccattcat ttttcgctct ctcagatacc ttgctgcgtg cataagtgag taagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca agtacgcgtt ggggccggtt atcggggagg gacagtttgg gagtgtgcgc atggcgacag tcaaggcgac ggggcagacg gttgccgtga aactgctaca tgtgcctcgg cttacggaag gcattccgca tccggtggcg cgggagcttc tcattgcctc tcgtgtcgac tcgccgtttc tggtgaagac catcgagatt acaccgtgcg ggtcccacat ggctgtggtg atggagcgct gtatggagga cctggcgtgt gtcttgcgca agtgtagtcc ggctcaccca cttccgctgc tgttgacgca gtcgtatctg cgcatgcttc tcaccgcgct gcacgcattg cactcgagcg gtattctgca ccgtgatgtg aaaccgtcga actgctttgt ttctgtggat gggtgcctga agctcggcga ctttggcctc agccgcccac taggctcaga catgtcacac gaggttgtgt cgcgctggta ccgcgcgccg gagctgctct ttggccagcg acgctacggg ggcgaggtgg acatgtgggc ggctgggtgc gtcttcgccg aactcctgcg tggtcacggc cgccctttct ttacaggcga tggggatatc agccagattg cgaagatatt cgacgtcttc ggcaccccca cgggcgcaac atgtagtttc taccgtcagc tgcctgactg ggagaaggtt tatttcgaag agaagaaggg aactggcctg cgtgtgctgc tgccctttgt gccccctgag gcgcttgact tgctgacaaa aatgctagcc ctggacccgg catctcgctg cagcgccgca gaggcgctca cccattcatt tttcgctctc tcagatacct tgctgcgtgc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat