SSGCID: Target Page

LemaA.10201.a

methionyl-tRNA synthetase

CENTER ID:	LemaA.10201.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.10201.a.B1.GE42686	full length		1	747

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.10201.a.B1.PW38569	full length		1	747

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmjF.21.0810
UniProt:	Q4QCD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKFFTEKAN HQALKAVLCA VFVQKPLEVT LGSTYSTPYL QLPKSRALLY SCNEAARLIW STHAAPLPAD NSLEGEAEWL EWESTVLTPA LTPLYTHRRI TGEAEAALKK LDSTIEEHQG TVAVKGAPSS TSFLVDSVLF ASLLPALCEG GLLPAAQAAR VPHLVKWFQA FQAEHAELIA SAFDVLSVQE CGDFLRVPQT YEVSPKKQKV FFATTPIYYV NASPHIGHVY STLIVDVLGR YHRVKGEEVF VMTGTDEHGQ KVAEAAAKQG VSPMDFTTSV SSEFKQCFQE MNYDMNYFIR TTNPTHEKLV QDIWKKLAAK GDIYLGKYEG WYSVSDESFL TAQNVADGVD RDGKPCKVSL ESGHVVTWVE EENYMFRLSA FRERLLKYFH DHPNCIVPEF RRREVIKTVE KGLFDLSISR KRESVMNWSI PVPGDERHCI YVWLDALFNY YTGALTRVAT DGTETLDEDH HALNRWPADV HVVGKDILKF HAIYWPAFLM SAELPLPERL VSHGWWTKDH KKISKSLGNA FDPVEKAKEF GIDALKYFLM RESNFQDDGD YSDKNMVARL NGELADTLGN LVSRCVAPKI NVNGMWPEPA EYSESDKTLI ASLNNLAGTV DHYYCLPDIQ HALIAIFDVL RSLNAYVTEN APWKLVKMDT ARLGTVLYVT MEGLRICTMF LQPVMPQKAK EIMDALGVPE AARVGMENYL FGIVKPGTKI AGLAEGQVVF QKVTLPTEEG ERSSKGQ

NT Sequence

atgctcaagt ttttcacaga gaaggcgaat catcaggccc tcaaggccgt actttgcgcc gtgtttgtgc agaagcccct cgaggtgacg ctgggcagca cctacagcac gccgtacctg cagctgccca agtcgagggc gcttctgtac agctgcaatg aggcggcgcg tcttatctgg agcacgcacg ccgcgccgtt gcccgccgac aactcgctcg aaggagaggc ggaatggctc gagtgggagt cgacggtgct gacgccagcc ttgacccccc tctacacgca ccgacgcatc acgggcgagg ctgaggctgc cctgaagaag cttgactcca ccattgagga gcaccagggc actgtggcgg tgaagggggc gccgtcgtct acgtctttcc tagtcgacag cgtcctcttc gcgtccctcc tgccggctct gtgcgaaggt gggctgctcc ccgccgcgca ggcggcgagg gtgccgcatt tggtgaagtg gttccaggct ttccaggcgg agcacgcgga gctgattgcc tctgcgtttg atgtgctgtc ggtgcaagag tgcggcgact tcttgcgggt gccgcagacg tacgaggtga gccccaagaa gcagaaagtc ttcttcgcca cgacgccgat ctactacgtg aacgcgtcgc cacacatcgg ccacgtctac agcacgctca tcgtcgacgt tctcggccgc tatcaccgag tgaagggaga ggaggtgttc gtgatgacag gcacggacga gcacggtcag aaggtggccg aggcggcggc gaagcagggg gtatcgccga tggacttcac aacgagcgtg tcgagcgagt tcaagcagtg cttccaggag atgaattatg acatgaacta cttcatccgc acgacgaacc cgacgcatga gaagctggtt caggacatct ggaagaagct ggcggcgaag ggcgacatct acctcggcaa gtacgagggc tggtactcgg tgagcgacga gtcattcctg acggcgcaga acgtggcgga cggtgtagac agggatggaa agccgtgcaa ggtcagcctg gagagcggcc acgtcgtgac gtgggtggag gaggagaact acatgttccg tcttagcgcg ttccgcgagc gtctcctcaa gtacttccat gaccatccca actgcatcgt cccggagttc cgccgtcgcg aggtgatcaa gacggtcgag aagggtctct tcgacttgtc catctcccgc aagcgcgagt ccgtcatgaa ctggtccatc ccggtccccg gtgatgagcg ccactgcatc tacgtgtggc tggatgccct cttcaactac tacaccggcg ccctcacccg tgtcgcgacc gacggcaccg agacactgga cgaggaccac cacgcactga accggtggcc ggccgacgtg cacgtggtcg gcaaggacat cctcaagttt cacgccattt actggccggc cttcctgatg tccgcggaac tgccgctgcc ggagcggctg gtgtcgcatg gctggtggac aaaagatcac aagaagatca gcaagtcgct cggcaacgcc ttcgacccgg tggaaaaggc gaaggagttc ggcatcgacg cgctcaaata cttcctgatg cgcgagtcga acttccagga tgatggcgat tacagtgaca agaacatggt ggcccgcctg aatggcgaac tcgccgacac gctcggcaac ttggtgtcgc gctgtgtggc gccgaagatc aatgtgaacg gtatgtggcc ggagcccgcg gagtacagcg agagcgacaa gacgctgatc gcctcgctca acaacctggc tggcacggtg gatcactatt actgcctgcc tgacattcag cacgcgttga ttgccatctt tgacgtgctc cgcagcctca acgcctatgt gaccgagaac gcaccctgga agctggtgaa aatggatacg gcgcgtctcg gcacagtgct gtacgtaacg atggaggggc tgcgcatctg caccatgttc ctgcagccgg tgatgccgca gaaggcgaag gagattatgg acgcgctcgg ggtgccggag gcggcgcgcg tgggcatgga aaactacctg ttcggcattg taaagccggg gacgaagatt gctggcttgg cggagggcca agtcgtcttc cagaaggtga cactcccgac cgaggagggg gagcgcagct ccaaagggca gtaa

Details for LemaA.10201.a.B1.GE42686

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	85kDa
OBSERVED MW:	85kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML KFFTEKANHQ ALKAVLCAVF VQKPLEVTLG STYSTPYLQL PKSRALLYSC NEAARLIWST HAAPLPADNS LEGEAEWLEW ESTVLTPALT PLYTHRRITG EAEAALKKLD STIEEHQGTV AVKGAPSSTS FLVDSVLFAS LLPALCEGGL LPAAQAARVP HLVKWFQAFQ AEHAELIASA FDVLSVQECG DFLRVPQTYE VSPKKQKVFF ATTPIYYVNA SPHIGHVYST LIVDVLGRYH RVKGEEVFVM TGTDEHGQKV AEAAAKQGVS PMDFTTSVSS EFKQCFQEMN YDMNYFIRTT NPTHEKLVQD IWKKLAAKGD IYLGKYEGWY SVSDESFLTA QNVADGVDRD GKPCKVSLES GHVVTWVEEE NYMFRLSAFR ERLLKYFHDH PNCIVPEFRR REVIKTVEKG LFDLSISRKR ESVMNWSIPV PGDERHCIYV WLDALFNYYT GALTRVATDG TETLDEDHHA LNRWPADVHV VGKDILKFHA IYWPAFLMSA ELPLPERLVS HGWWTKDHKK ISKSLGNAFD PVEKAKEFGI DALKYFLMRE SNFQDDGDYS DKNMVARLNG ELADTLGNLV SRCVAPKINV NGMWPEPAEY SESDKTLIAS LNNLAGTVDH YYCLPDIQHA LIAIFDVLRS LNAYVTENAP WKLVKMDTAR LGTVLYVTME GLRICTMFLQ PVMPQKAKEI MDALGVPEAA RVGMENYLFG IVKPGTKIAG LAEGQVVFQK VTLPTEEGER SSKGQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca agtttttcac agagaaggcg aatcatcagg ccctcaaggc cgtactttgc gccgtgtttg tgcagaagcc cctcgaggtg acgctgggca gcacctacag cacgccgtac ctgcagctgc ccaagtcgag ggcgcttctg tacagctgca atgaggcggc gcgtcttatc tggagcacgc acgccgcgcc gttgcccgcc gacaactcgc tcgaaggaga ggcggaatgg ctcgagtggg agtcgacggt gctgacgcca gccttgaccc ccctctacac gcaccgacgc atcacgggcg aggctgaggc tgccctgaag aagcttgact ccaccattga ggagcaccag ggcactgtgg cggtgaaggg ggcgccgtcg tctacgtctt tcctagtcga cagcgtcctc ttcgcgtccc tcctgccggc tctgtgcgaa ggtgggctgc tccccgccgc gcaggcggcg agggtgccgc atttggtgaa gtggttccag gctttccagg cggagcacgc ggagctgatt gcctctgcgt ttgatgtgct gtcggtgcaa gagtgcggcg acttcttgcg ggtgccgcag acgtacgagg tgagccccaa gaagcagaaa gtcttcttcg ccacgacgcc gatctactac gtgaacgcgt cgccacacat cggccacgtc tacagcacgc tcatcgtcga cgttctcggc cgctatcacc gagtgaaggg agaggaggtg ttcgtgatga caggcacgga cgagcacggt cagaaggtgg ccgaggcggc ggcgaagcag ggggtatcgc cgatggactt cacaacgagc gtgtcgagcg agttcaagca gtgcttccag gagatgaatt atgacatgaa ctacttcatc cgcacgacga acccgacgca tgagaagctg gttcaggaca tctggaagaa gctggcggcg aagggcgaca tctacctcgg caagtacgag ggctggtact cggtgagcga cgagtcattc ctgacggcgc agaacgtggc ggacggtgta gacagggatg gaaagccgtg caaggtcagc ctggagagcg gccacgtcgt gacgtgggtg gaggaggaga actacatgtt ccgtcttagc gcgttccgcg agcgtctcct caagtacttc catgaccatc ccaactgcat cgtcccggag ttccgccgtc gcgaggtgat caagacggtc gagaagggtc tcttcgactt gtccatctcc cgcaagcgcg agtccgtcat gaactggtcc atcccggtcc ccggtgatga gcgccactgc atctacgtgt ggctggatgc cctcttcaac tactacaccg gcgccctcac ccgtgtcgcg accgacggca ccgagacact ggacgaggac caccacgcac tgaaccggtg gccggccgac gtgcacgtgg tcggcaagga catcctcaag tttcacgcca tttactggcc ggccttcctg atgtccgcgg aactgccgct gccggagcgg ctggtgtcgc atggctggtg gacaaaagat cacaagaaga tcagcaagtc gctcggcaac gccttcgacc cggtggaaaa ggcgaaggag ttcggcatcg acgcgctcaa atacttcctg atgcgcgagt cgaacttcca ggatgatggc gattacagtg acaagaacat ggtggcccgc ctgaatggcg aactcgccga cacgctcggc aacttggtgt cgcgctgtgt ggcgccgaag atcaatgtga acggtatgtg gccggagccc gcggagtaca gcgagagcga caagacgctg atcgcctcgc tcaacaacct ggctggcacg gtggatcact attactgcct gcctgacatt cagcacgcgt tgattgccat ctttgacgtg ctccgcagcc tcaacgccta tgtgaccgag aacgcaccct ggaagctggt gaaaatggat acggcgcgtc tcggcacagt gctgtacgta acgatggagg ggctgcgcat ctgcaccatg ttcctgcagc cggtgatgcc gcagaaggcg aaggagatta tggacgcgct cggggtgccg gaggcggcgc gcgtgggcat ggaaaactac ctgttcggca ttgtaaagcc ggggacgaag attgctggct tggcggaggg ccaagtcgtc ttccagaagg tgacactccc gaccgaggag ggggagcgca gctccaaagg gcagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LemaA.10201.a.B1.PW38569

PURIFICATION DATe:	11/19/2018
CONCENTRATION:	53.03mg/ml
OBSERVED MW:	80kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCA AGTTTTTCAC AGAGAAGGCG AATCATCAGG CCCTCAAGGC CGTACTTTGC GCCGTGTTTG TGCAGAAGCC CCTCGAGGTG ACGCTGGGCA GCACCTACAG CACGCCGTAC CTGCAGCTGC CCAAGTCGAG GGCGCTTCTG TACAGCTGCA ATGAGGCGGC GCGTCTTATC TGGAGCACGC ACGCCGCGCC GTTGCCCGCC GACAACTCGC TCGAAGGAGA GGCGGAATGG CTCGAGTGGG AGTCGACGGT GCTGACGCCA GCCTTGACCC CCCTCTACAC GCACCGACGC ATCACGGGCG AGGCTGAGGC TGCCCTGAAG AAGCTTGACT CCACCATTGA GGAGCACCAG GGCACTGTGG CGGTGAAGGG GGCGCCGTCG TCTACGTCTT TCCTAGTCGA CAGCGTCCTC TTCGCGTCCC TCCTGCCGGC TCTGTGCGAA GGTGGGCTGC TCCCCGCCGC GCAGGCGGCG AGGGTGCCGC ATTTGGTGAA GTGGTTCCAG GCTTTCCAGG CGGAGCACGC GGAGCTGATT GCCTCTGCGT TTGATGTGCT GTCGGTGCAA GAGTGCGGCG ACTTCTTGCG GGTGCCGCAG ACGTACGAGG TGAGCCCCAA GAAGCAGAAA GTCTTCTTCG CCACGACGCC GATCTACTAC GTGAACGCGT CGCCACACAT CGGCCACGTC TACAGCACGC TCATCGTCGA CGTTCTCGGC CGCTATCACC GAGTGAAGGG AGAGGAGGTG TTCGTGATGA CAGGCACGGA CGAGCACGGT CAGAAGGTGG CCGAGGCGGC GGCGAAGCAG GGGGTATCGC CGATGGACTT CACAACGAGC GTGTCGAGCG AGTTCAAGCA GTGCTTCCAG GAGATGAATT ATGACATGAA CTACTTCATC CGCACGACGA ACCCGACGCA TGAGAAGCTG GTTCAGGACA TCTGGAAGAA GCTGGCGGCG AAGGGCGACA TCTACCTCGG CAAGTACGAG GGCTGGTACT CGGTGAGCGA CGAGTCATTC CTGACGGCGC AGAACGTGGC GGACGGTGTA GACAGGGATG GAAAGCCGTG CAAGGTCAGC CTGGAGAGCG GCCACGTCGT GACGTGGGTG GAGGAGGAGA ACTACATGTT CCGTCTTAGC GCGTTCCGCG AGCGTCTCCT CAAGTACTTC CATGACCATC CCAACTGCAT CGTCCCGGAG TTCCGCCGTC GCGAGGTGAT CAAGACGGTC GAGAAGGGTC TCTTCGACTT GTCCATCTCC CGCAAGCGCG AGTCCGTCAT GAACTGGTCC ATCCCGGTCC CCGGTGATGA GCGCCACTGC ATCTACGTGT GGCTGGATGC CCTCTTCAAC TACTACACCG GCGCCCTCAC CCGTGTCGCG ACCGACGGCA CCGAGACACT GGACGAGGAC CACCACGCAC TGAACCGGTG GCCGGCCGAC GTGCACGTGG TCGGCAAGGA CATCCTCAAG TTTCACGCCA TTTACTGGCC GGCCTTCCTG ATGTCCGCGG AACTGCCGCT GCCGGAGCGG CTGGTGTCGC ATGGCTGGTG GACAAAAGAT CACAAGAAGA TCAGCAAGTC GCTCGGCAAC GCCTTCGACC CGGTGGAAAA GGCGAAGGAG TTCGGCATCG ACGCGCTCAA ATACTTCCTG ATGCGCGAGT CGAACTTCCA GGATGATGGC GATTACAGTG ACAAGAACAT GGTGGCCCGC CTGAATGGCG AACTCGCCGA CACGCTCGGC AACTTGGTGT CGCGCTGTGT GGCGCCGAAG ATCAATGTGA ACGGTATGTG GCCGGAGCCC GCGGAGTACA GCGAGAGCGA CAAGACGCTG ATCGCCTCGC TCAACAACCT GGCTGGCACG GTGGATCACT ATTACTGCCT GCCTGACATT CAGCACGCGT TGATTGCCAT CTTTGACGTG CTCCGCAGCC TCAACGCCTA TGTGACCGAG AACGCACCCT GGAAGCTGGT GAAAATGGAT ACGGCGCGTC TCGGCACAGT GCTGTACGTA ACGATGGAGG GGCTGCGCAT CTGCACCATG TTCCTGCAGC CGGTGATGCC GCAGAAGGCG AAGGAGATTA TGGACGCGCT CGGGGTGCCG GAGGCGGCGC GCGTGGGCAT GGAAAACTAC CTGTTCGGCA TTGTAAAGCC GGGGACGAAG ATTGCTGGCT TGGCGGAGGG CCAAGTCGTC TTCCAGAAGG TGACACTCCC GACCGAGGAG GGGGAGCGCA GCTCCAAAGG GCAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT