SSGCID: Target Page

LemaA.01621.a

casein kinase I, isoform 2

CENTER ID:	LemaA.01621.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.01621.a.B1.GE34438	full length		1	353

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF35.1010
RefSeq:	XP_843210.1
UniProt:	Q9NHE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNVELRVGNR YRIGQKIGSG SFGEIFRGTN IQTGDPVAIK LEQVKTRHPQ LTYESRFYRI LGSGGGAVGI PMMFYHGVEG EFNVMVIELL GPSLEDLFSF CGRRLSLKTT LMLADQMISR IEFVHSKSVL HRDIKPDNFL MGTGKKGHHV YIIDFGLAKK YRDPRTHAHI PYKEGKSLTG TARYCSINTH MGVEQGRRDD MEGIGYILMY FLRGSLPWQG LKAHTKQEKY NRISERKQTT PVELLCKGFP SEFAAYMNYV RALRFEDKPD YSYLKRMFRD LFVREGYHVD YVFDWTLKRI HESLQEQQSF PGGSNGGGAA GNGSPVNQSP AQGGNGGAPN SANNQESGAP EQQ

NT Sequence

atgaacgtgg aactgcgcgt cggcaaccgc taccggatcg gtcagaagat cggctccggc tccttcggtg agatcttccg cggcacgaac attcagacgg gagaccctgt cgccatcaag ctcgagcagg tgaagacgcg ccacccgcag ctcacgtatg agtcgcgctt ctatcgcatc ctcggcagcg gcggcggcgc cgtcggcatc cccatgatgt tctatcacgg cgtcgagggc gagttcaacg tgatggtgat tgagctgctc ggcccctcgc tggaagacct cttttccttt tgcggtcgcc gcctctcgct gaagacgacg ctgatgctgg cggaccagat gatcagccgc atcgagtttg tgcatagcaa gagcgttctg caccgcgaca tcaagcccga caatttcctc atgggcaccg gaaagaaggg ccaccacgtg tacatcatcg actttgggct ggcgaagaag tatcgtgacc cccgcacgca cgcccacatc ccatacaagg agggcaagag cctgacaggc acggcccgct actgtagtat caacacgcac atgggcgtgg agcagggccg ccgtgacgac atggagggca tcggctacat cctcatgtac ttccttcgtg gatcgctgcc atggcagggc ttgaaggcgc acacgaagca ggagaagtac aaccgcattt ccgagcgaaa gcagacgaca ccggtggagc tgctgtgcaa gggcttcccc agcgagttcg ccgcctacat gaactacgtg cgtgcgctgc gcttcgagga caagcccgac tactcgtacc tgaagcgcat gttccgcgac ctatttgtgc gcgagggata ccacgtcgac tacgtcttcg actggacgct gaagcgcatt catgagagtc tgcaggagca gcagtcgttc ccgggcggca gcaacggtgg cggtgctgcg ggcaatggct ccccggtgaa ccaaagcccg gcacagggcg gcaacggtgg tgccccaaac agcgctaaca accaggaatc gggtgcgcca gagcagcagt ag

Details for LemaA.01621.a.B1.GE34438

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMN VELRVGNRYR IGQKIGSGSF GEIFRGTNIQ TGDPVAIKLE QVKTRHPQLT YESRFYRILG SGGGAVGIPM MFYHGVEGEF NVMVIELLGP SLEDLFSFCG RRLSLKTTLM LADQMISRIE FVHSKSVLHR DIKPDNFLMG TGKKGHHVYI IDFGLAKKYR DPRTHAHIPY KEGKSLTGTA RYCSINTHMG VEQGRRDDME GIGYILMYFL RGSLPWQGLK AHTKQEKYNR ISERKQTTPV ELLCKGFPSE FAAYMNYVRA LRFEDKPDYS YLKRMFRDLF VREGYHVDYV FDWTLKRIHE SLQEQQSFPG GSNGGGAAGN GSPVNQSPAQ GGNGGAPNSA NN

Validated NT Sequence

ctggttgtta gcgctgtttg gggcaccacc gttgccgccc tgtgccgggc tttggttcac cggggagcca ttgcccgcag caccgccacc gttgctgccg cccgggaacg actgctgctc ctgcagactc tcatgaatgc gcttcagcgt ccagtcgaag acgtagtcga cgtggtatcc ctcgcgcaca aataggtcgc ggaacatgcg cttcaggtac gagtagtcgg gcttgtcctc gaagcgcagc gcacgcacgt agttcatgta ggcggcgaac tcgctgggga agcccttgca cagcagctcc accggtgtcg tctgctttcg ctcggaaatg cggttgtact tctcctgctt cgtgtgcgcc ttcaagccct gccatggcag cgatccacga aggaagtaca tgaggatgta gccgatgccc tccatgtcgt cacggcggcc ctgctccacg cccatgtgcg tgttgatact acagtagcgg gccgtgcctg tcaggctctt gccctccttg tatgggatgt gggcgtgcgt gcgggggtca cgatacttct tcgccagccc aaagtcgatg atgtacacgt ggtggccctt ctttccggtg cccatgagga aattgtcggg cttgatgtcg cggtgcagaa cgctcttgct atgcacaaac tcgatgcggc tgatcatctg gtccgccagc atcagcgtcg tcttcagcga gaggcggcga ccgcaaaagg aaaagaggtc ttccagcgag gggccgagca gctcaatcac catcacgttg aactcgccct cgacgccgtg atagaacatc atggggatgc cgacggcgcc gccgccgctg ccgaggatgc gatagaagcg cgactcatac gtgagctgcg ggtggcgcgt cttcacctgc tcgagcttga tggcgacagg gtctcccgtc tgaatgttcg tgccgcggaa gatctcaccg aaggagccgg agccgatctt ctgaccgatc cggtagcggt tgccgacgcg cagttccacg ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg tggaactgcg cgtcggcaac cgctaccgga tcggtcagaa gatcggctcc ggctccttcg gtgagatctt ccgcggcacg aacattcaga cgggagaccc tgtcgccatc aagctcgagc aggtgaagac gcgccacccg cagctcacgt atgagtcgcg cttctatcgc atcctcggca gcggcggcgg cgccgtcggc atccccatga tgttctatca cggcgtcgag ggcgagttca acgtgatggt gattgagctg ctcggcccct cgctggaaga cctcttttcc ttttgcggtc gccgcctctc gctgaagacg acgctgatgc tggcggacca gatgatcagc cgcatcgagt ttgtgcatag caagagcgtt ctgcaccgcg acatcaagcc cgacaatttc ctcatgggca ccggaaagaa gggccaccac gtgtacatca tcgactttgg gctggcgaag aagtatcgtg acccccgcac gcacgcccac atcccataca aggagggcaa gagcctgaca ggcacggccc gctactgtag tatcaacacg cacatgggcg tggagcaggg ccgccgtgac gacatggagg gcatcggcta catcctcatg tacttccttc gtggatcgct gccatggcag ggcttgaagg cgcacacgaa gcaggagaag tacaaccgca tttccgagcg aaagcagacg acaccggtgg agctgctgtg caagggcttc cccagcgagt tcgccgccta catgaactac gtgcgtgcgc tgcgcttcga ggacaagccc gactactcgt acctgaagcg catgttccgc gacctatttg tgcgcgaggg ataccacgtc gactacgtct tcgactggac gctgaagcgc attcatgaga gtctgcagga gcagcagtcg ttcccgggcg gcagcaacgg tggcggtgct gcgggcaatg gctccccggt gaaccaaagc ccggcacagg gcggcaacgg tggtgcccca aacagcgcta acaaccagga atcgggtgcg ccagagcagc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat