LemaA.01510.b

Repressor of differentiation kinase 2, putative (RDK2)

CENTER ID: LemaA.01510.b
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.01510.b.B2.GE42801 PHoranyi kinase domain truncation 33 441
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.31.2960
UniProt: Q4Q5W2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEQDQRKPN GGNNGLPVPP STTETGQGPL QDILGRTPNC QYVKKRLLGQ GSFGSAWRVE ESATGNVFAA KVMDLNNMSE KDRGFVTNEV KCLSRCCNPH VVRCENAIER EGQLLIVMEY ADGGDLYKQI KARVREMKFF REHEVVFIFL QLCLALDHIH SNKMMHRDLK TANILLTTTG LVKLGDFGFS RQYEDSLSNA VGSTFCGTPY YLSPELWHRQ PYSKKSEMWA LGVVLYEVMA LRRPFTGKNM DELITNICHG RCSDLPSSYS ASLRDVCNRL LSVDPNTRPT IHELFHEPFI RSNLETLKRS VEKHNRIPAD VREAIARCIT QSLNTEEPPA HLEEPEPLIG TVKRHTALRG WQKCLLSFDE KQISIRDPSG VDPEEVVPVA TLTSVCPIDV MMAGEEFVFA MKNQAGKAFW FMAPSQRAYE QWLTTLQEAA P
NT Sequence
atggaggagc aagaccagcg aaaaccgaac ggcggcaaca acggcctgcc agtgccaccc agcacgacgg agaccggtca gggccccctg caggacatcc tcggccgaac gccaaactgc cagtacgtca agaagcgctt gctgggacag ggcagctttg gtagtgcgtg gcgcgtggag gaaagtgcga ccggcaacgt cttcgccgcg aaggtgatgg acctgaataa catgagcgag aaggatcgcg gatttgtgac aaacgaggtg aagtgcctct cgcgctgctg caacccgcac gtggtgcgtt gcgagaacgc catcgagaga gaggggcagc tgttgattgt gatggagtac gccgatggcg gggatctgta caagcagatc aaggcccgcg tgcgcgagat gaagtttttc cgagagcacg aggtggtgtt catctttctg cagctctgcc ttgccctgga tcacatccac tcgaacaaga tgatgcaccg cgacctgaag accgcaaaca tactcctcac taccaccggc ctcgtcaagc tcggcgactt cggcttttcg cgccagtacg aggactccct ctccaatgcc gtcggctcga ccttctgtgg cacgccatac tacctcagcc cggagttgtg gcatcgccag ccgtacagca agaagagtga gatgtgggcc ctcggggtcg tgctttacga ggtcatggcg ctccgccgcc ccttcaccgg caagaacatg gatgagctca tcacaaacat atgccacggc cggtgctctg acctgccgtc atcgtactca gcgagtttgc gcgatgtgtg caaccgactt ctctccgtcg accccaacac gcggccgacg attcacgagc tgtttcacga gccttttatt cgctccaacc tcgagaccct caagcgcagt gtggagaagc acaaccgcat ccccgccgac gtgcgtgagg ccatcgcgcg ctgcattacg cagtcgctga acacggagga accgcctgcg cacttggaag agccggagcc gctcattggc acggtgaagc gccacacggc gctgcgcgga tggcagaagt gcctcttgag cttcgacgag aagcagattt cgatccggga cccaagtggg gtggacccgg aagaggtggt gccggtcgcc actctgacct ccgtgtgtcc catcgatgtc atgatggctg gcgaggagtt tgtctttgcc atgaagaacc aggcaggcaa ggccttttgg ttcatggcgc cgagccagag ggcgtacgaa cagtggctga ctacgttgca ggaggccgcc ccgtaa
Details for LemaA.01510.b.B2.GE42801
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 47kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI LGRTPNCQYV KXXLLGQGSF XXAWRVEESX TGXVFAAKVM DLNNMSEKDR GFVTNEVXXL SRCCNPHVVR CENAIEREGQ LLIVMEYADG GDLYKQIKAR VREMKFFREH XXVFIFLQLC LALDHIHSNK MMHRDLXTAN ILLTTTGXVK LGDXXFSRQY EDSLSNAVGS TFCGTPXXLS PELWHRQPYS KKSEMWALGV VLYXXMALRR PFTGKNMDEL ITNICHGRCS DLPSSYSASL RDVCNRLLSV DPNTRPTIHE LFHEPFIRXN LETLKRSVEK HNRIPADVRE AIARCITQSL NTEEPPAHLE EPEPLIGTVK RHTALRGWQK CLLSFXEKQI SIRDPSGVDP EEVXXVATXT SVCPIDVMMA GEEFVFAMKN QAGKAFWFMA PSQRAYEQW
Validated NT Sequence
agccactgtt cgtacgccct ctggctcggc gccatgaacc aaaaggcctt gcctgcctgg ttcttcatgg caaagacaaa ctcctcgcca gccatcatga catcgatggg acacacggag gtcngngtgg cgaccgnnnn nacctcttcc gggtccaccc cacttgggtc ccggatcgaa atctgcttct cgtngaagct caagaggcac ttctgccatc cgcgcagcgc cgtgtggcgc ttcaccgtgc caatgagcgg ctccggctct tccaagtgcg caggcggttc ctccgtgttc agcgactgcg taatgcagcg cgcgatggcc tcacgcacgt cggcggggat gcggttgtgc ttctccacac tgcgcttgag ggtctcgagg ttggngcgaa taaaaggctc gtgaaacagc tcgtgaatcg tcggccgcgt gttggggtcg acggagagaa gtcggttgca cacatcgcgc aaactcgctg agtacgatga cggcaggtca gagcaccggc cgtggcatat gtttgtgatg agctcatcca tgttcttgcc ggtgaagggg cggcggagcg ccatnnnnnc gtaaagcacg accccgaggg cccacatctc actcttcttg ctgtacggct ggcgatgcca caactccggg ctgaggnnnt ntggcgtgcc acagaaggtc gagccgacgg cattggagag ggagtcctcg tactggcgcg aaaagnnnaa gtcgccgagc ttgacnangc cggtggtagt gaggagtatg tttgcggtcn tcaggtcgcg gtgcatcatc ttgttcgagt ggatgtgatc cagggcaagg cagagctgca gaaagatgaa caccannncg tgctctcgga aaaacttcat ctcgcgcacg cgngccttga tctgcttgta cagatccccg ccatcggcgt actccatcac aatcaacagc tgcccctctc tctcgatggc gttctcgcaa cgcaccacgt gcgggttgca gcagcgcgag aggcnnttca cctcgtttgt cacaaatccg cgatccttct cgctcatgtt attcaggtcc atcaccttcg cggcgaagac gnnnccggtn ncactttcct ccacgcgcca cgcannnnca aagctgccct gtcccagcaa nngnttcttg acgtactggc agtttggcgt tcggccgagg atcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHIL GRTPNCQYVK KRLLGQGSFG SAWRVEESAT GNVFAAKVMD LNNMSEKDRG FVTNEVKCLS RCCNPHVVRC ENAIEREGQL LIVMEYADGG DLYKQIKARV REMKFFREHE VVFIFLQLCL ALDHIHSNKM MHRDLKTANI LLTTTGLVKL GDFGFSRQYE DSLSNAVGST FCGTPYYLSP ELWHRQPYSK KSEMWALGVV LYEVMALRRP FTGKNMDELI TNICHGRCSD LPSSYSASLR DVCNRLLSVD PNTRPTIHEL FHEPFIRSNL ETLKRSVEKH NRIPADVREA IARCITQSLN TEEPPAHLEE PEPLIGTVKR HTALRGWQKC LLSFDEKQIS IRDPSGVDPE EVVPVATLTS VCPIDVMMAG EEFVFAMKNQ AGKAFWFMAP SQRAYEQWLT TLQEAAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcctcg gccgaacgcc aaactgccag tacgtcaaga agcgcttgct gggacagggc agctttggta gtgcgtggcg cgtggaggaa agtgcgaccg gcaacgtctt cgccgcgaag gtgatggacc tgaataacat gagcgagaag gatcgcggat ttgtgacaaa cgaggtgaag tgcctctcgc gctgctgcaa cccgcacgtg gtgcgttgcg agaacgccat cgagagagag gggcagctgt tgattgtgat ggagtacgcc gatggcgggg atctgtacaa gcagatcaag gcccgcgtgc gcgagatgaa gtttttccga gagcacgagg tggtgttcat ctttctgcag ctctgccttg ccctggatca catccactcg aacaagatga tgcaccgcga cctgaagacc gcaaacatac tcctcactac caccggcctc gtcaagctcg gcgacttcgg cttttcgcgc cagtacgagg actccctctc caatgccgtc ggctcgacct tctgtggcac gccatactac ctcagcccgg agttgtggca tcgccagccg tacagcaaga agagtgagat gtgggccctc ggggtcgtgc tttacgaggt catggcgctc cgccgcccct tcaccggcaa gaacatggat gagctcatca caaacatatg ccacggccgg tgctctgacc tgccgtcatc gtactcagcg agtttgcgcg atgtgtgcaa ccgacttctc tccgtcgacc ccaacacgcg gccgacgatt cacgagctgt ttcacgagcc ttttattcgc tccaacctcg agaccctcaa gcgcagtgtg gagaagcaca accgcatccc cgccgacgtg cgtgaggcca tcgcgcgctg cattacgcag tcgctgaaca cggaggaacc gcctgcgcac ttggaagagc cggagccgct cattggcacg gtgaagcgcc acacggcgct gcgcggatgg cagaagtgcc tcttgagctt cgacgagaag cagatttcga tccgggaccc aagtggggtg gacccggaag aggtggtgcc ggtcgccact ctgacctccg tgtgtcccat cgatgtcatg atggctggcg aggagtttgt ctttgccatg aagaaccagg caggcaaggc cttttggttc atggcgccga gccagagggc gtacgaacag tggctgacta cgttgcagga ggccgccccg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat