SSGCID: Target Page

LemaA.01510.a

protein kinase A catalytic subunit

CENTER ID:	LemaA.01510.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.01510.a.B1.GE34450	full length		1	371

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF35.4010
RefSeq:	XP_843507.1
UniProt:	Q9GNR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSAAKDSYP AESSGHLNNE KAQLSKIPSS HPDPSKVTVA PNVKAAYYFA KPDTSSWKLS DFELKNTLGT GSFGRVRIAH RKGTEEYYAI KCLRKREIIK MKQQQHVAQE KGILMELCHP FIVNMMCSFQ DEKKVYFLLE FVMGGEMFTH LRTAGRFPND VAKFYHAELV LAFEYLHSLD VIYRDLKPEN LLLDNKGHVK MTDFGFAKKV PDRTFTLCGT PEYLAPEVIQ SKGHGKAVDW WTMGVLLYEF IAGYPPFYDD TPFRIYEKIL AGRLKFPNWF DGRARDLVKG LLQTDHTKRL GTLKGGPADV KNHPYFHGAN WDKLYARYYP APIPVRVKSP GDTSNFEKYP DSPVDRTPAL TSAQQAEFNG F

NT Sequence

atgtcgtctg ctgccaagga cagttatccg gcggagtcgt cgggccacct caacaacgag aaggcgcagc tgtcgaagat tccctccagt catccagacc cctcgaaggt gactgtcgcg ccaaacgtca aggccgctta ttacttcgca aagccagaca cctccagctg gaaactctca gatttcgaac tgaagaacac gctcggcacc ggttccttcg gccgcgtgcg catcgcccac cgcaagggca cggaggagta ctacgcgatc aagtgcctga gaaagcgcga gatcatcaag atgaagcagc agcagcacgt tgcgcaggaa aaggggatcc taatggagct gtgtcacccg ttcatcgtga acatgatgtg ctccttccag gacgagaaga aggtgtactt tctgctggag tttgtcatgg gcggcgagat gttcacgcac ctgcgcactg ccgggcggtt cccgaatgac gttgcgaagt tctaccacgc ggagctggtg cttgcgttcg agtatctgca ctcgctggac gtgatctacc gcgatctgaa gccggagaat ctgctgctgg acaacaaggg gcatgtgaag atgacggact ttgggtttgc gaagaaggtg ccggaccgga cgttcacgct gtgcgggaca ccggagtacc ttgcgccgga ggtgatccag agcaagggcc acgggaaggc ggtggactgg tggacgatgg gcgtgctgct gtacgaattc attgccggat acccgccgtt ctacgacgac acgccgttcc ggatttacga gaagatcctt gctgggcggc tgaagttccc gaactggttt gacggccgcg cgcgcgacct cgtgaagggg ctgctgcaga cggaccatac gaagcgcctg gggacgctga aaggcgggcc ggcggacgtg aagaaccatc cgtacttcca cggtgcgaac tgggacaagc tgtacgcacg ttactacccc gcgccgatcc ctgtgcgggt gaagagccct ggcgacacga gcaacttcga gaagtacccg gacagcccgg tcgaccgcac gcctgcgctg acatcggcgc agcaggccga attcaatggc ttttag

Details for LemaA.01510.a.B1.GE34450

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS SAAKDSYPAE SSGHLNNEKA QLSKIPSSHP DPSKVTVAPN VKAAYYFAKP DTSSWKLSDF ELKNTLGTGS FGRVRIAHRK GTEEYYAIKC LRKREIIKMK QQQHVAQEKG ILMELCHPFI VNMMCSFQDE KKVYFLLEFV MGGEMFTHLR TAGRFPNDVA KFYHAELVLA FEYLHSLDVI YRDLKPENLL LDNKGHVKMT DFGFAKKVPD RTFTLCGTPE YLAPEVIQSK GHGKAVDWWT MGVLLYEFIA GYPPFYDDTP FRIYEKILAG RLKFPNWFDG RARDLVKGLL QTDHTKRLGT LKGGPADVKN HPYFHGANWD KLYARYYPAP IPVRVKSPGD TSNFEKYPDS PVDRTPALTS AQQAEFNGF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt ctgctgccaa ggacagttat ccggcggagt cgtcgggcca cctcaacaac gagaaggcgc agctgtcgaa gattccctcc agtcatccag acccctcgaa ggtgactgtc gcgccaaacg tcaaggccgc ttattacttc gcaaagccag acacctccag ctggaaactc tcagatttcg aactgaagaa cacgctcggc accggttcct tcggccgcgt gcgcatcgcc caccgcaagg gcacggagga gtactacgcg atcaagtgcc tgagaaagcg cgagatcatc aagatgaagc agcagcagca cgttgcgcag gaaaagggga tcctaatgga gctgtgtcac ccgttcatcg tgaacatgat gtgctccttc caggacgaga agaaggtgta ctttctgctg gagtttgtca tgggcggcga gatgttcacg cacctgcgca ctgccgggcg gttcccgaat gacgttgcga agttctacca cgcggagctg gtgcttgcgt tcgagtatct gcactcgctg gacgtgatct accgcgatct gaagccggag aatctgctgc tggacaacaa ggggcatgtg aagatgacgg actttgggtt tgcgaagaag gtgccggacc ggacgttcac gctgtgcggg acaccggagt accttgcgcc ggaggtgatc cagagcaagg gccacgggaa ggcggtggac tggtggacga tgggcgtgct gctgtacgaa ttcattgccg gatacccgcc gttctacgac gacacgccgt tccggattta cgagaagatc cttgctgggc ggctgaagtt cccgaactgg tttgacggcc gcgcgcgcga cctcgtgaag gggctgctgc agacggacca tacgaagcgc ctggggacgc tgaaaggcgg gccggcggac gtgaagaacc atccgtactt ccacggtgcg aactgggaca agctgtacgc acgttactac cccgcgccga tccctgtgcg ggtgaagagc cctggcgaca cgagcaactt cgagaagtac ccggacagcc cggtcgaccg cacgcctgcg ctgacatcgg cgcagcaggc cgaattcaat ggcttttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t