LemaA.01471.b
aspartate aminotransferase, putative
| CENTER ID: | LemaA.01471.b |
| ORGANISM: | Leishmania major Friedlin |
| ASSOCIATED DISEASE: | Cutaneous leishmaniasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.01471.b.B1.GE34449 | full length | 1 | 412 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.01471.b.B1.PW35059 | full length | 1 | 412 |
Structures
4H51
DEPOSITED: 9/18/2012
DETERMINATION: XRay
CLONE: LemaA.01471.b.B1.GE34449
PROTEIN: LemaA.01471.b.B1.PW35059
Publications by SSGCID
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Clifton MC, Edwards TE, Lorimer DD, Lukacs CM, Myler P, Staker BL, van Voorhis W, Wall A
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 566-71
PMID: 25945710; PMCID: PMC4427166
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:LmjF35.0820 |
| OrthoMCL: | OG5_126737 |
| RefSeq: | XP_843191.1 |
| UniProt: | Q4FX34 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTQAAMTTA ERWQKIQAQA PDVIFDLAKR AAAAKGPKAN LVIGAYRDEQ GRPYPLRVVR KAEQLLLDMN LDYEYLPISG YQPFIDEAVK IIYGNTVELE NLVAVQTLSG TGAVSLGAKL LTRVFDAETT PIYLSDPTWP NHYGVVKAAG WKNICTYAYY DPKTVSLNFE GMKKDILAAP DGSVFILHQC AHNPTGVDPS QEQWNEIASL MLAKHHQVFF DSAYQGYASG SLDTDAYAAR LFARRGIEVL LAQSFSKNMG LYSERAGTLS LLLKDKTKRA DVKSVMDSLI REEYTCPPAH GARLAHLILS NNELRKEWEA ELSAMAERIR TMRRTVYDEL LRLQTPGSWE HVINQIGMFS FLGLSKAQCE YCQNHNIFIT VSGRANMAGL THETALMLAQ TINDAVRNVN RE
NT Sequence
atgtccacgc aggcggccat gaccacggcg gagcgctggc agaagattca ggcacaagct cccgatgtca tcttcgatct cgcaaaacgc gccgccgctg ccaagggccc caaggccaac ctcgtcattg gtgcctaccg cgacgagcag ggccgtccct atccgctacg cgtggtccgc aaggctgagc agcttctctt ggacatgaat ctcgactacg agtacctacc catctcgggc taccagccct tcatcgatga ggcggtaaag attatctacg gcaataccgt cgagctggag aacctggttg cggtgcagac gctgagcggg accggtgctg tctctctcgg ggcgaagctg ctgactcgcg tcttcgacgc tgagacgacg cccatctacc tttccgaccc cacgtggccc aaccactacg gcgtcgtgaa ggctgctggc tggaagaaca tctgcacgta cgcctactac gaccccaaga cggtcagcct gaatttcgag ggcatgaaga aagacattct ggcggcgccg gacggctccg tgttcattct gcaccagtgc gcgcacaacc ccaccggcgt ggacccgtcg caggagcagt ggaacgagat cgcgtcactg atgctggcca agcaccatca ggtgttcttc gactccgcct accaaggcta tgcgagcggc agcctcgaca cggacgcgta tgctgcccgc ctgtttgccc gccgcggcat cgaggtactg ctggcgcagt cgttctccaa gaacatgggc ttgtacagcg agcgtgcagg cacgctgtcg ctgctcctca aggacaagac gaagcgcgcg gatgtaaaga gcgtgatgga ttcgctgatc cgtgaggagt acacgtgccc cccagcccac ggtgcccgct tagcccacct aatcctgagc aacaacgaac tgcgaaagga gtgggaggca gagctatcag ccatggcaga gcgcatccgt acgatgcgcc gcaccgtgta cgacgagctg ctgcgcctgc agacgcccgg gagctgggaa catgtcatta accagattgg catgttttcc ttcctcgggc tgtcaaaggc gcagtgcgaa tactgccaaa accacaacat cttcatcaca gtgtcgggcc gcgctaacat ggcaggtctg acgcatgaga cggcgctgat gctagcacag acgatcaacg atgctgtgcg caatgtgaat cgtgagtga
Details for LemaA.01471.b.B1.GE34449
| HARVESTED ON: | 2/6/2012 |
| SEQUENCED ON: | 2/21/2012 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MTTAERWQKI QAQAPDVIFD LAKRAAAAKG PKANLVIGAY RDEQGRPYPL RVVRKAEQLL LDMNLDYEYL PISGYQPFID EAVKIIYGNT VELENLVAVQ TLSGTGAVSL GAKLLTRVFD AETTPIYLSD PTWPNHYGVV KAAGWKNICT YAYYDPKTVS LNFEGMKKDI LAAPDGSVFI LHQCAHNPTG VDPSQEQWNE IASLMLAKHH QVFFDSAYQG YASGSLDTDA YAARLFARRG IEVLLAQSFS KNMGLYSERA GTLSLLLKDK TKRADVKSVM DSLIREEYTC PPAHGARLAH LILSNNELRK EWEAELSAMA ERIRTMRRTV YDELLRLQTP GSWEHVINQI GMFSFLGLSK AQCEYCQNHN IFITVSGRAN MAGLTHETAL MLAQTI
Validated NT Sequence
ttgatcgtct gtgctagcat cagcgccgtc tcatgcgtca gacctgccat gttagcgcgg cccgacactg tgatgaagat gttgtggttt tggcagtatt cgcactgcgc ctttgacagc ccgaggaagg aaaacatgcc aatctggtta atgacatgtt cccagctccc gggcgtctgc aggcgcagca gctcgtcgta cacggtgcgg cgcatcgtac ggatgcgctc tgccatggct gatagctctg cctcccactc ctttcgcagt tcgttgttgc tcaggattag gtgggctaag cgggcaccgt gggctggggg gcacgtgtac tcctcacgga tcagcgaatc catcacgctc tttacatccg cgcgcttcgt cttgtccttg aggagcagcg acagcgtgcc tgcacgctcg ctgtacaagc ccatgttctt ggagaacgac tgcgccagca gtacctcgat gccgcggcgg gcaaacaggc gggcagcata cgcgtccgtg tcgaggctgc cgctcgcata gccttggtag gcggagtcga agaacacctg atggtgcttg gccagcatca gtgacgcgat ctcgttccac tgctcctgcg acgggtccac gccggtgggg ttgtgcgcgc actggtgcag aatgaacacg gagccgtccg gcgccgccag aatgtctttc ttcatgccct cgaaattcag gctgaccgtc ttggggtcgt agtaggcgta cgtgcagatg ttcttccagc cagcagcctt cacgacgccg tagtggttgg gccacgtggg gtcggaaagg tagatgggcg tcgtctcagc gtcgaagacg cgagtcagca gcttcgcccc gagagagaca gcaccggtcc cgctcagcgt ctgcaccgca accaggttct ccagctcgac ggtattgccg tagataatct ttaccgcctc atcgatgaag ggctggtagc ccgagatggg taggtactcg tagtcgagat tcatgtccaa gagaagctgc tcagccttgc ggaccacgcg tagcggatag ggacggccct gctcgtcgcg gtaggcacca atgacgaggt tggccttggg gcccttggca gcggcggcgc gttttgcgag atcgaagatg acatcgggag cttgtgcctg aatcttctgc cagcgctccg ccgtggtcat ggccgcctgc gtg
Expected Protein Sequence
MAHHHHHHMS TQAAMTTAER WQKIQAQAPD VIFDLAKRAA AAKGPKANLV IGAYRDEQGR PYPLRVVRKA EQLLLDMNLD YEYLPISGYQ PFIDEAVKII YGNTVELENL VAVQTLSGTG AVSLGAKLLT RVFDAETTPI YLSDPTWPNH YGVVKAAGWK NICTYAYYDP KTVSLNFEGM KKDILAAPDG SVFILHQCAH NPTGVDPSQE QWNEIASLML AKHHQVFFDS AYQGYASGSL DTDAYAARLF ARRGIEVLLA QSFSKNMGLY SERAGTLSLL LKDKTKRADV KSVMDSLIRE EYTCPPAHGA RLAHLILSNN ELRKEWEAEL SAMAERIRTM RRTVYDELLR LQTPGSWEHV INQIGMFSFL GLSKAQCEYC QNHNIFITVS GRANMAGLTH ETALMLAQTI NDAVRNVNRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca cgcaggcggc catgaccacg gcggagcgct ggcagaagat tcaggcacaa gctcccgatg tcatcttcga tctcgcaaaa cgcgccgccg ctgccaaggg ccccaaggcc aacctcgtca ttggtgccta ccgcgacgag cagggccgtc cctatccgct acgcgtggtc cgcaaggctg agcagcttct cttggacatg aatctcgact acgagtacct acccatctcg ggctaccagc ccttcatcga tgaggcggta aagattatct acggcaatac cgtcgagctg gagaacctgg ttgcggtgca gacgctgagc gggaccggtg ctgtctctct cggggcgaag ctgctgactc gcgtcttcga cgctgagacg acgcccatct acctttccga ccccacgtgg cccaaccact acggcgtcgt gaaggctgct ggctggaaga acatctgcac gtacgcctac tacgacccca agacggtcag cctgaatttc gagggcatga agaaagacat tctggcggcg ccggacggct ccgtgttcat tctgcaccag tgcgcgcaca accccaccgg cgtggacccg tcgcaggagc agtggaacga gatcgcgtca ctgatgctgg ccaagcacca tcaggtgttc ttcgactccg cctaccaagg ctatgcgagc ggcagcctcg acacggacgc gtatgctgcc cgcctgtttg cccgccgcgg catcgaggta ctgctggcgc agtcgttctc caagaacatg ggcttgtaca gcgagcgtgc aggcacgctg tcgctgctcc tcaaggacaa gacgaagcgc gcggatgtaa agagcgtgat ggattcgctg atccgtgagg agtacacgtg ccccccagcc cacggtgccc gcttagccca cctaatcctg agcaacaacg aactgcgaaa ggagtgggag gcagagctat cagccatggc agagcgcatc cgtacgatgc gccgcaccgt gtacgacgag ctgctgcgcc tgcagacgcc cgggagctgg gaacatgtca ttaaccagat tggcatgttt tccttcctcg ggctgtcaaa ggcgcagtgc gaatactgcc aaaaccacaa catcttcatc acagtgtcgg gccgcgctaa catggcaggt ctgacgcatg agacggcgct gatgctagca cagacgatca acgatgctgt gcgcaatgtg aatcgtgagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LemaA.01471.b.B1.PW35059
| PURIFICATION DATe: | 3/21/2012 |
| CONCENTRATION: | 53.1mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 19 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTTAERWQKI QAQAPDVIFD LAKRAAAAKG PKANLVIGAY RDEQGRPYPL RVVRKAEQLL LDMNLDYEYL PISGYQPFID EAVKIIYGNT VELENLVAVQ TLSGTGAVSL GAKLLTRVFD AETTPIYLSD PTWPNHYGVV KAAGWKNICT YAYYDPKTVS LNFEGMKKDI LAAPDGSVFI LHQCAHNPTG VDPSQEQWNE IASLMLAKHH QVFFDSAYQG YASGSLDTDA YAARLFARRG IEVLLAQSFS KNMGLYSERA GTLSLLLKDK TKRADVKSVM DSLIREEYTC PPAHGARLAH LILSNNELRK EWEAELSAMA ERIRTMRRTV YDELLRLQTP GSWEHVINQI GMFSFLGLSK AQCEYCQNHN IFITVSGRAN MAGLTHETAL MLAQTI
Validated NT Sequence
ttgatcgtct gtgctagcat cagcgccgtc tcatgcgtca gacctgccat gttagcgcgg cccgacactg tgatgaagat gttgtggttt tggcagtatt cgcactgcgc ctttgacagc ccgaggaagg aaaacatgcc aatctggtta atgacatgtt cccagctccc gggcgtctgc aggcgcagca gctcgtcgta cacggtgcgg cgcatcgtac ggatgcgctc tgccatggct gatagctctg cctcccactc ctttcgcagt tcgttgttgc tcaggattag gtgggctaag cgggcaccgt gggctggggg gcacgtgtac tcctcacgga tcagcgaatc catcacgctc tttacatccg cgcgcttcgt cttgtccttg aggagcagcg acagcgtgcc tgcacgctcg ctgtacaagc ccatgttctt ggagaacgac tgcgccagca gtacctcgat gccgcggcgg gcaaacaggc gggcagcata cgcgtccgtg tcgaggctgc cgctcgcata gccttggtag gcggagtcga agaacacctg atggtgcttg gccagcatca gtgacgcgat ctcgttccac tgctcctgcg acgggtccac gccggtgggg ttgtgcgcgc actggtgcag aatgaacacg gagccgtccg gcgccgccag aatgtctttc ttcatgccct cgaaattcag gctgaccgtc ttggggtcgt agtaggcgta cgtgcagatg ttcttccagc cagcagcctt cacgacgccg tagtggttgg gccacgtggg gtcggaaagg tagatgggcg tcgtctcagc gtcgaagacg cgagtcagca gcttcgcccc gagagagaca gcaccggtcc cgctcagcgt ctgcaccgca accaggttct ccagctcgac ggtattgccg tagataatct ttaccgcctc atcgatgaag ggctggtagc ccgagatggg taggtactcg tagtcgagat tcatgtccaa gagaagctgc tcagccttgc ggaccacgcg tagcggatag ggacggccct gctcgtcgcg gtaggcacca atgacgaggt tggccttggg gcccttggca gcggcggcgc gttttgcgag atcgaagatg acatcgggag cttgtgcctg aatcttctgc cagcgctccg ccgtggtcat ggccgcctgc gtg
Expressed Protein Sequence
MAHHHHHHMS TQAAMTTAER WQKIQAQAPD VIFDLAKRAA AAKGPKANLV IGAYRDEQGR PYPLRVVRKA EQLLLDMNLD YEYLPISGYQ PFIDEAVKII YGNTVELENL VAVQTLSGTG AVSLGAKLLT RVFDAETTPI YLSDPTWPNH YGVVKAAGWK NICTYAYYDP KTVSLNFEGM KKDILAAPDG SVFILHQCAH NPTGVDPSQE QWNEIASLML AKHHQVFFDS AYQGYASGSL DTDAYAARLF ARRGIEVLLA QSFSKNMGLY SERAGTLSLL LKDKTKRADV KSVMDSLIRE EYTCPPAHGA RLAHLILSNN ELRKEWEAEL SAMAERIRTM RRTVYDELLR LQTPGSWEHV INQIGMFSFL GLSKAQCEYC QNHNIFITVS GRANMAGLTH ETALMLAQTI NDAVRNVNRE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCA CGCAGGCGGC CATGACCACG GCGGAGCGCT GGCAGAAGAT TCAGGCACAA GCTCCCGATG TCATCTTCGA TCTCGCAAAA CGCGCCGCCG CTGCCAAGGG CCCCAAGGCC AACCTCGTCA TTGGTGCCTA CCGCGACGAG CAGGGCCGTC CCTATCCGCT ACGCGTGGTC CGCAAGGCTG AGCAGCTTCT CTTGGACATG AATCTCGACT ACGAGTACCT ACCCATCTCG GGCTACCAGC CCTTCATCGA TGAGGCGGTA AAGATTATCT ACGGCAATAC CGTCGAGCTG GAGAACCTGG TTGCGGTGCA GACGCTGAGC GGGACCGGTG CTGTCTCTCT CGGGGCGAAG CTGCTGACTC GCGTCTTCGA CGCTGAGACG ACGCCCATCT ACCTTTCCGA CCCCACGTGG CCCAACCACT ACGGCGTCGT GAAGGCTGCT GGCTGGAAGA ACATCTGCAC GTACGCCTAC TACGACCCCA AGACGGTCAG CCTGAATTTC GAGGGCATGA AGAAAGACAT TCTGGCGGCG CCGGACGGCT CCGTGTTCAT TCTGCACCAG TGCGCGCACA ACCCCACCGG CGTGGACCCG TCGCAGGAGC AGTGGAACGA GATCGCGTCA CTGATGCTGG CCAAGCACCA TCAGGTGTTC TTCGACTCCG CCTACCAAGG CTATGCGAGC GGCAGCCTCG ACACGGACGC GTATGCTGCC CGCCTGTTTG CCCGCCGCGG CATCGAGGTA CTGCTGGCGC AGTCGTTCTC CAAGAACATG GGCTTGTACA GCGAGCGTGC AGGCACGCTG TCGCTGCTCC TCAAGGACAA GACGAAGCGC GCGGATGTAA AGAGCGTGAT GGATTCGCTG ATCCGTGAGG AGTACACGTG CCCCCCAGCC CACGGTGCCC GCTTAGCCCA CCTAATCCTG AGCAACAACG AACTGCGAAA GGAGTGGGAG GCAGAGCTAT CAGCCATGGC AGAGCGCATC CGTACGATGC GCCGCACCGT GTACGACGAG CTGCTGCGCC TGCAGACGCC CGGGAGCTGG GAACATGTCA TTAACCAGAT TGGCATGTTT TCCTTCCTCG GGCTGTCAAA GGCGCAGTGC GAATACTGCC AAAACCACAA CATCTTCATC ACAGTGTCGG GCCGCGCTAA CATGGCAGGT CTGACGCATG AGACGGCGCT GATGCTAGCA CAGACGATCA ACGATGCTGT GCGCAATGTG AATCGTGAGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT