SSGCID: Target Page

LemaA.01418.a

peptidyl-prolyl cis-trans isomerase-like protein

CENTER ID:	LemaA.01418.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.01418.a.B1.GE40690	full length		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF24.1315
RefSeq:	XP_001683648.1
UniProt:	Q4QAK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIALEFPLPP STSLFLSVFV IDNRYSAFPE SEEIRLVVLP EPQLTMPPRK AIAKMKSHSA ESVDVDLQNC LLRIDKGTDV YGMLVIELDS GRSPKACELV GQNIPASNTT DKSRGKQGVY KNCRFSRLTK EGIQTGEVVP AAKPIPAAEL EGEIGRVPHC YGAVSLCRSS TSFDGSQFFI CLTSDSVELD HLNKKHVCFG RVVEGHDVLA ALQSDLAEYS GDMGLVRKDC PYVMAELSYA SM

NT Sequence

atgattgcat tggagtttcc tttgcctcca tcgacatctc tttttctatc cgttttcgtg atcgacaacc gatactctgc tttcccagaa tcggaagaaa tacgactagt tgtgcttccc gaacctcaac tcacaatgcc tccgagaaag gcaatagcaa agatgaagtc gcacagcgct gagagtgttg atgtcgacct ccagaactgt ttgctgcgca tcgataaggg caccgacgtg tatgggatgt tggtgattga gttggacagt ggccgttctc ccaaggcgtg cgagctagtt ggtcaaaaca ttcctgcgtc aaacacaacc gacaaaagtc gtggaaagca gggtgtttac aagaactgcc gtttttcgcg cctgaccaaa gaaggaattc agactggcga agttgttccg gctgccaaac ctattcccgc agccgagctg gagggcgaga taggtcgcgt gccgcactgc tacggtgccg tatcgctgtg tcgcagctcg acctctttcg acggatcgca attttttatc tgtctcacca gcgattctgt ggagctcgat cacttaaaca agaagcacgt ttgctttgga cgagttgtcg agggtcatga tgtcttggca gctttgcaga gtgatttggc cgaatattct ggcgacatgg gcctagtccg taaggactgc ccttatgtga tggcggagct cagctacgca tcgatgtaa

Details for LemaA.01418.a.B1.GE40690

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI ALEFPLPPST SLFLSVFVID NRYSAFPESE EIRLVVLPEP QLTMPPRKAI AKMKSHSAES VDVDLQNCLL RIDKGTDVYG MLVIELDSGR SPKACELVGQ NIPASNTTDK SRGKQGVYKN CRFSRLTKEG IQTGEVVPAA KPIPAAELEG EIGRVPHCYG AVSLCRSSTS FDGSQFFICL TSDSVELDHL NKKHVCFGRV VEGHDVLAAL QSDLAEYSGD MGLVRKDCPY VMAELSYASM

Validated NT Sequence

atggctcacc accaccacca ccatatgatt gcattggagt ttcctttgcc tccatcgaca tctctttttc tatccgtttt cgtgatcgac aaccgatact ctgctttccc agaatcggaa gaaatacgac tagttgtgct tcccgaacct caactcacaa tgcctccgag aaaggcaata gcaaagatga agtcgcacag cgctgagagt gttgatgtcg acctccagaa ctgtttgctg cgcatcgata agggcaccga cgtgtatggg atgttggtga ttgagttgga cagtggccgt tctcccaagg cgtgcgagct agttggtcaa aacattcctg cgtcaaacac aaccgacaaa agtcgtggaa agcagggtgt ttacaagaac tgccgttttt cgcgcctgac caaagaagga attcagactg gcgaagttgt tccggctgcc aaacctattc ccgcagccga gctggagggc gagataggtc gcgtgccgca ctgctacggt gccgtatcgc tgtgtcgcag ctcgacctct ttcgacggat cgcaattttt tatctgtctc accagcgatt ctgtggagct cgatcactta aacaagaagc acgtttgctt tggacgagtt gtcgagggtc atgatgtctt ggcagctttg cagagtgatt tggccgaata ttctggcgac atgggcctag tccgtaagga ctgcccttat gtgatggcgg agctcagcta cgcatcgatg taagtgagta agat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg cattggagtt tcctttgcct ccatcgacat ctctttttct atccgttttc gtgatcgaca accgatactc tgctttccca gaatcggaag aaatacgact agttgtgctt cccgaacctc aactcacaat gcctccgaga aaggcaatag caaagatgaa gtcgcacagc gctgagagtg ttgatgtcga cctccagaac tgtttgctgc gcatcgataa gggcaccgac gtgtatggga tgttggtgat tgagttggac agtggccgtt ctcccaaggc gtgcgagcta gttggtcaaa acattcctgc gtcaaacaca accgacaaaa gtcgtggaaa gcagggtgtt tacaagaact gccgtttttc gcgcctgacc aaagaaggaa ttcagactgg cgaagttgtt ccggctgcca aacctattcc cgcagccgag ctggagggcg agataggtcg cgtgccgcac tgctacggtg ccgtatcgct gtgtcgcagc tcgacctctt tcgacggatc gcaatttttt atctgtctca ccagcgattc tgtggagctc gatcacttaa acaagaagca cgtttgcttt ggacgagttg tcgagggtca tgatgtcttg gcagctttgc agagtgattt ggccgaatat tctggcgaca tgggcctagt ccgtaaggac tgcccttatg tgatggcgga gctcagctac gcatcgatgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat