LemaA.01408.a

tryptophanyl-tRNA synthetase, putative

CENTER ID: LemaA.01408.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.01408.a.B2.GE42543 soluble domain 27 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.23.0300
UniProt: Q4QBE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDGARQHMR DVMAKCRKRT MTTTPSANAE GVATPVLDAV PAGDAALQAA ASPAMQQRQQ PPALALHHFF HRDIAFSHRD LHKALVDIEA SVKTGERSVF LYTGRGPSAG TMHLGHVLPF MLTKYLQDVF SLPLVIQITD DEKFLFRDVP FEGAKADELI RSNIKDIIAF NFNPRHTFIF RNTHYMGDMY PTVLRLQRSM TGNAVKHTLG ITDSDNVGKL AFPATQAAPC FSTAFRRVLR NGDRPMRCLI PCAIDQDPFF VLTRAAALRL KQLPPALLHT KFLPALKGLE HKMSSSAEEK GVITLHDTDK QVRKKLRRAF SGGCATLEQM QETGANLELD VAYQYLRFFC PDDTLFADVT QRYRSGTLNS GEVKDLAADC IIREVLHDWR ERRATVTDDD VVEFCRIRDI LL
NT Sequence
atggacgacg gtgcgcgtca gcacatgcgc gatgtcatgg ccaagtgccg taagcggacc atgaccacca ccccatccgc taacgccgag ggcgtcgcga ccccggtttt ggatgccgtg ccagctggag atgcggcact gcaggccgcc gcatctcctg ccatgcagca gcggcagcag ccaccagcgc tggcgcttca ccacttcttc caccgcgaca tcgccttctc acaccgcgat ttgcacaagg cacttgtgga tatcgaggcc tccgtcaaaa cgggagagcg ctctgtcttc ctctacactg gccgtggccc cagcgctggc accatgcact tgggccatgt gctgcccttc atgctcacca agtacctcca agacgtcttc agccttccgc tcgttataca aatcacagat gacgaaaagt ttctctttcg cgacgtcccc ttcgagggtg ccaaagcgga cgagctgatt cgcagcaaca tcaaggatat catcgccttc aacttcaacc cgcgccacac cttcatattc cgcaacacgc actacatggg cgatatgtac ccgacagtgc tccggctgca acgtagcatg acaggcaacg ccgtgaagca cacgctcggc atcacggaca gcgataatgt cggcaagctt gcctttcctg ccacccaggc tgctccgtgc ttcagcaccg ctttccgtcg tgttctgcgg aacggcgaca ggccgatgcg ctgcctgatt ccctgcgcca ttgatcagga ccccttcttc gttctcacgc gcgccgccgc gctgcggctg aagcagctac cgccggccct gcttcacacc aagttcctgc cggcgctcaa aggcctggag cacaagatga gcagcagtgc agaggagaag ggcgtcatta ctctacatga caccgataaa caggtgcgca agaagctgcg ccgcgccttt tcgggcggct gcgccaccct agagcagatg caagagacgg gcgccaacct ggagttggat gtggcgtacc agtacctgcg gttcttctgc cctgatgaca cactcttcgc cgacgtgacg cagcgctacc gtagcggtac cctgaatagc ggagaggtga aggacctggc ggcggattgc atcatccgtg aggtgctgca cgattggcgg gagcggcgcg caacggtgac ggacgatgac gtggtggagt tctgccgtat ccgtgacatt cttctctga
Details for LemaA.01408.a.B2.GE42543
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 44kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA NAEGVATPVL DAVPAGDAAL QAAASPAMQQ RQQPPALALH HFFHRDIAFS HRDLHKALVD IEASVKTGER SVFLYTGRGP SAGTMHLGHV LPFMLTKYLQ DVFSLPLVIQ ITDDEKFLFR DVPFEGAKAD ELIRSNIKDI IAFNFNPRHT FIFRNTHYMG DMYPTVLRLQ RSMTGNAVKH TLGITDSDNV GKLAFPATQA APCFSTAFRR VLRNGDRPMR CLIPCAIDQD PFFVLTRAAA LRLKQLPPAL LHTKFLPALK GLEHKMSSSA EEKGVITLHD TDKQVRKKLR RAFSGGCATL EQMQETGANL ELDVAYQYLR FFCPDDTLFA DVTQRYRSGT LNSGEVKDLA ADCIIREVLH DWRERRATVT DDDVVEF
Validated NT Sequence
atggctcacc accaccacca ccatatggct aacgccgagg gcgtcgcgac cccggttttg gatgccgtgc cagctggaga tgcggcactg caggccgccg catctcctgc catgcagcag cggcagcagc caccagcgct ggcgcttcac cacttcttcc accgcgacat cgccttctca caccgcgatt tgcacaaggc acttgtggat atcgaggcct ccgtcaaaac gggagagcgc tctgtcttcc tctacactgg ccgtggcccc agcgctggca ccatgcactt gggccatgtg ctgcccttca tgctcaccaa gtacctccaa gacgtcttca gccttccgct cgttatacaa atcacagatg acgaaaagtt tctctttcgc gacgtcccct tcgagggtgc caaagcggac gagctgattc gcagcaacat caaggatatc atcgccttca acttcaaccc gcgccacacc ttcatattcc gcaacacgca ctacatgggc gatatgtacc cgacagtgct ccggctgcaa cgtagcatga caggcaacgc cgtgaagcac acgctcggca tcacggacag cgataatgtc ggcaagcttg cctttcctgc cacccaggct gctccgtgct tcagcaccgc tttccgtcgt gttctgcgga acggcgacag gccgatgcgc tgcctgattc cctgcgccat tgatcaggac cccttcttcg ttctcacgcg cgccgccgcg ctgcggctga agcagctacc gccggccctg cttcacacca agttcctgcc ggcgctcaaa ggcctggagc acaagatgag cagcagtgca gaggagaagg gcgtcattac tctacatgac accgataaac aggtgcgcaa gaagctgcgc cgcgcctttt cgggcggctg cgccacccta gagcagatgc aagagacggg cgccaacctg gagttggatg tggcgtacca gtacctgcgg ttcttctgcc ctgatgacac actcttcgcc gacgtgacgc agcgctaccg tagcggtacc ctgaatagcg gagaggtgaa ggacctggcg gcggattgca tcatccgtga ggtgctgcac gattggcggg agcggcgcgc aacggtgacg gacgatgacg tggtggagtt ct
Expected Protein Sequence
MAHHHHHHAN AEGVATPVLD AVPAGDAALQ AAASPAMQQR QQPPALALHH FFHRDIAFSH RDLHKALVDI EASVKTGERS VFLYTGRGPS AGTMHLGHVL PFMLTKYLQD VFSLPLVIQI TDDEKFLFRD VPFEGAKADE LIRSNIKDII AFNFNPRHTF IFRNTHYMGD MYPTVLRLQR SMTGNAVKHT LGITDSDNVG KLAFPATQAA PCFSTAFRRV LRNGDRPMRC LIPCAIDQDP FFVLTRAAAL RLKQLPPALL HTKFLPALKG LEHKMSSSAE EKGVITLHDT DKQVRKKLRR AFSGGCATLE QMQETGANLE LDVAYQYLRF FCPDDTLFAD VTQRYRSGTL NSGEVKDLAA DCIIREVLHD WRERRATVTD DDVVEFCRIR DILL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctaacg ccgagggcgt cgcgaccccg gttttggatg ccgtgccagc tggagatgcg gcactgcagg ccgccgcatc tcctgccatg cagcagcggc agcagccacc agcgctggcg cttcaccact tcttccaccg cgacatcgcc ttctcacacc gcgatttgca caaggcactt gtggatatcg aggcctccgt caaaacggga gagcgctctg tcttcctcta cactggccgt ggccccagcg ctggcaccat gcacttgggc catgtgctgc ccttcatgct caccaagtac ctccaagacg tcttcagcct tccgctcgtt atacaaatca cagatgacga aaagtttctc tttcgcgacg tccccttcga gggtgccaaa gcggacgagc tgattcgcag caacatcaag gatatcatcg ccttcaactt caacccgcgc cacaccttca tattccgcaa cacgcactac atgggcgata tgtacccgac agtgctccgg ctgcaacgta gcatgacagg caacgccgtg aagcacacgc tcggcatcac ggacagcgat aatgtcggca agcttgcctt tcctgccacc caggctgctc cgtgcttcag caccgctttc cgtcgtgttc tgcggaacgg cgacaggccg atgcgctgcc tgattccctg cgccattgat caggacccct tcttcgttct cacgcgcgcc gccgcgctgc ggctgaagca gctaccgccg gccctgcttc acaccaagtt cctgccggcg ctcaaaggcc tggagcacaa gatgagcagc agtgcagagg agaagggcgt cattactcta catgacaccg ataaacaggt gcgcaagaag ctgcgccgcg ccttttcggg cggctgcgcc accctagagc agatgcaaga gacgggcgcc aacctggagt tggatgtggc gtaccagtac ctgcggttct tctgccctga tgacacactc ttcgccgacg tgacgcagcg ctaccgtagc ggtaccctga atagcggaga ggtgaaggac ctggcggcgg attgcatcat ccgtgaggtg ctgcacgatt ggcgggagcg gcgcgcaacg gtgacggacg atgacgtggt ggagttctgc cgtatccgtg acattcttct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat