LemaA.01391.a

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID: LemaA.01391.a
ORGANISM: Leishmania major Friedlin
ASSOCIATED DISEASE: Cutaneous leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemaA.01391.a.HZ2.GE43718 kinase domain (Jensen) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmjF.36.0720
UniProt: Q4Q204

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAEIESHIL KKYEIQAQLG QGAYGIVWRA LERKHNRVVA LKKIYDAFQN STDAQRTFRE IMFLHRLHHP NIIKLLHVHR AFNDRDIYLV FEYMETDLHV VIRANILEEI HKQFIIYQLL KTMKYLHSAE ILHRDMKPSN LLVNSDCTMK VADFGLARSI LSLEGEQASR PVLTDYIATR WYRPPEILLG STRYTKGVDM WSVGCILAEL MLGKPIFPGR STTNQLELIC SVTGMPSAAD VAATNSQFAH AMLRDIHCAH RRTFAELLPS ASADALDLIE RFMRFNPNRR ISAAEALEHP YVAAFHRPDE EAVAPEPITV SLPDSQRLPL AKYRDAIYEQ IAALRRSSTS ADQRQRAERQ TAGSTASRKT SVSSTAARAA PQRSAVKPTS TSAVSEASSS KAYARPAFRS ATSAASGLES RPVAREAAVR K
NT Sequence
atgtcggcgg aaattgagtc gcatattctg aagaagtatg agattcaagc acagctagga cagggtgcct acggcatcgt ttggcgcgcc ctggagcgca aacacaaccg cgtcgttgca ctcaagaaaa tttacgacgc cttccaaaac tcgaccgatg cgcagcgcac cttccgcgag atcatgtttc tgcaccggct gcaccacccg aacatcatca aactgctgca cgtccatcgt gccttcaacg atcgcgacat atacctggtc ttcgaataca tggagaccga cctgcacgtg gtgattaggg caaacatctt ggaggaaatc cacaaacagt tcatcatcta ccagctgctc aagacgatga agtacctgca ttctgctgag attcttcatc gtgacatgaa accaagcaat ctgctggtaa acagcgactg cacaatgaag gtggctgact tcggtctcgc gcgctctatc ctgtcccttg agggcgagca ggcgtcacgg ccggtgctaa cggactacat tgcgacacgg tggtaccgcc cgccggagat tttgctcggt tcgacacgat acaccaaggg cgtagatatg tggtcggtgg gctgcattct tgcggagttg atgctgggaa agcccatctt tcccggccgc tccaccacga accagttgga gctgatctgc agcgtgacgg gcatgccctc cgcggcggat gtggcggcga ccaactcgca gtttgctcac gcgatgcttc gcgacatcca ctgcgcgcat cgacgcacgt tcgctgagct gctgccgagc gcttctgccg atgcgctgga cctgatcgag cgctttatgc gtttcaaccc gaaccgtcgt atatctgccg ccgaggccct ggagcacccg tacgtcgctg cctttcaccg tcctgacgag gaagcggtgg caccggaacc catcaccgtc tctcttcccg acagccagcg tttgcccttg gccaagtaca gggacgccat ctacgagcaa attgccgcac tgcgccgtag ctccacgtcc gccgatcaac ggcagcgcgc ggagcgacag accgccggca gcaccgctag ccgcaagacg tccgtcagca gcaccgccgc acgggcggct ccccagcgca gtgctgtgaa gcccacctcg actagtgcgg tcagcgaagc atcatcttcg aaggcgtacg cccgtcccgc cttccgctcg gcaacgtctg ccgcctctgg tctggaaagt agacccgtcg cgcgtgaggc agcggtgcgc aagtga
Details for LemaA.01391.a.HZ2.GE43718
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 79
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMK SDSXVNQEAK PXVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS AEIESHILXK YEIQAQLGQG AYGIVWRALX RKHNRVVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IKLLHVHRAF NDRDIYLVFX YMETDLHVVI RANILEEIHK QFIIYQLLKT MKYXHSAEIL HRDMKPSNLL VNSDCTMKVA DFGLAXXILS LXGRAGVXAG ANGLHCDTVV PPAGDFARFD TIHQGRRYVV GGLHSCGVDA GKAHLSRPLH HEPVGADLQR DGHALRGGCG GDQLAVCSRD ASRHPLRASX HVR
Validated NT Sequence
ccgcgcgctg ccgttgatcg gcggacgtgg agctacggcg cagtgcggca atttgctcgt agatggcgtc cctgtacttg gccaagggca aacgctggct gtcgggaaga gagacggtga tgggttccgg tgccaccgct tcctcgtcag gacggtgaaa ggcagcgacg tacgggtgct ccagggcctc ggcggcagat atacgacggt tcgggttgaa acgcataaag cgctcgatca ggtccagcgc atcggcagaa gcgctcggca gcagctcagc gaacgtgcgn cgatgcgcgc agtggatgtc gcgaagcatc gcgtgagcaa actgcgagtt ggtcgccgcc acatccgccg cggagggcat gcccgtcacg ctgcagatca gctccaactg gttcgtggtg gagcggccgg gaaagatggg ctttcccagc atcaactccg caagaatgca gcccaccgac cacatatcta cgcccttggt gtatcgtgtc gaaccgagca aaatctccgg cgggcggnac caccgtgtcg caatgtagtc cgttagcacc ggccgngacg cctgctcgcc ctcnaaggga caggatagnn ngcgcgagac cgaagtcagc caccttcatt gtgcagtcgc tgtttaccag cagattgctt ggtttcatgt cacgatgaag aatctcagca gaatgcangt acttcatcgt cttgagcagc tggtagatga tgaactgttt gtggatttcc tccaagatgt ttgccctaat caccacgtgc aggtcggtct ccatgtattn gaagaccagg tatatgtcgc gatcgttgaa ggcacgatgg acgtgcagca gtttgatgat gttcgggtgg tgcagccggt gcagaaacat gatctcgcgg aaggtgcgct gcgcatcggt cgagttttgg aaggcgtcgt aaattttctt gagtgcaacg acgcggttgt gtttgcgctn cagggcgcgc caaacgatgc cgtaggcacc ctgtcctagc tgtgcttgaa tctcatactt nttcagaata tgcgactcaa tttccgccga catcgaacca ggaccctggg tctgagcttc cagggtacca ccaatctgtt ctctgtgagc ctcaataata tcgttatcct ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc tttaaattga tgtgagtctc aggcttgact tctggcttga cctntggctt agcttcttga ttgacttnng agtccgactt catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS AEIESHILKK YEIQAQLGQG AYGIVWRALE RKHNRVVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IKLLHVHRAF NDRDIYLVFE YMETDLHVVI RANILEEIHK QFIIYQLLKT MKYLHSAEIL HRDMKPSNLL VNSDCTMKVA DFGLARSILS LEGEQASRPV LTDYIATRWY RPPEILLGST RYTKGVDMWS VGCILAELML GKPIFPGRST TNQLELICSV TGMPSAADVA ATNSQFAHAM LRDIHCAHRR TFAELLPSAS ADALDLIERF MRFNPNRRIS AAEALEHPYV AAFHRPDEEA VAPEPITVSL PDSQRLPLAK YRDAIYEQIA ALRRSSTSAD QRQRAER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgtcggcgga aattgagtcg catattctga agaagtatga gattcaagca cagctaggac agggtgccta cggcatcgtt tggcgcgccc tggagcgcaa acacaaccgc gtcgttgcac tcaagaaaat ttacgacgcc ttccaaaact cgaccgatgc gcagcgcacc ttccgcgaga tcatgtttct gcaccggctg caccacccga acatcatcaa actgctgcac gtccatcgtg ccttcaacga tcgcgacata tacctggtct tcgaatacat ggagaccgac ctgcacgtgg tgattagggc aaacatcttg gaggaaatcc acaaacagtt catcatctac cagctgctca agacgatgaa gtacctgcat tctgctgaga ttcttcatcg tgacatgaaa ccaagcaatc tgctggtaaa cagcgactgc acaatgaagg tggctgactt cggtctcgcg cgctctatcc tgtcccttga gggcgagcag gcgtcacggc cggtgctaac ggactacatt gcgacacggt ggtaccgccc gccggagatt ttgctcggtt cgacacgata caccaagggc gtagatatgt ggtcggtggg ctgcattctt gcggagttga tgctgggaaa gcccatcttt cccggccgct ccaccacgaa ccagttggag ctgatctgca gcgtgacggg catgccctcc gcggcggatg tggcggcgac caactcgcag tttgctcacg cgatgcttcg cgacatccac tgcgcgcatc gacgcacgtt cgctgagctg ctgccgagcg cttctgccga tgcgctggac ctgatcgagc gctttatgcg tttcaacccg aaccgtcgta tatctgccgc cgaggccctg gagcacccgt acgtcgctgc ctttcaccgt cctgacgagg aagcggtggc accggaaccc atcaccgtct ctcttcccga cagccagcgt ttgcccttgg ccaagtacag ggacgccatc tacgagcaaa ttgccgcact gcgccgtagc tccacgtccg ccgatcaacg gcagcgcgcg gagcgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa