SSGCID: Target Page

LemaA.01375.a

cyclophilin, putative

CENTER ID:	LemaA.01375.a
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.01375.a.B1.GE34441	full length		1	229

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF22.1450
RefSeq:	XP_001683296.1
UniProt:	Q4QBK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MWRLSRVALQ SNRAALYMPY TPVATNPVVY FDITAEGDAL GRVSVELFRD VVPRTSENFR SLCTGERGYG QCLLYYKGTP FHRIIPGFVM QGGDILTKDG RSNVSVFGYP FPDESFEGKA GKHLPGTVGM AHSGPNQNGS QFFFNLGRNE QLDRKFVVVG QVLGGWEIVN QVVKLCGSRC GTPVSRAWIS DCGQSGGYMA EETQEALQGE RAQHVIPGKE VLDLIQPRY

NT Sequence

atgtggcgcc tctcgcgtgt ggcgctccag tcgaatcgcg cggcgctcta catgccgtac acccccgtag cgactaaccc ggtggtctat tttgacatca ccgccgaagg cgacgccctg gggcgcgtgt cggtggagct gtttagggac gttgtgccga gaacaagcga gaacttccgc tcgctgtgca ctggcgagcg cggatatggt cagtgcttgt tgtactacaa gggaaccccg tttcatcgca ttattccagg gtttgtcatg caaggcggcg acattctcac taaggacggc cgcagcaacg ttagcgtctt tggatatccg tttccggatg agtcttttga gggaaaggcc gggaagcact tgcccggcac tgtcggcatg gcgcacagtg ggccgaacca aaatggatcc cagtttttct tcaatctggg gcgcaatgag cagctcgatc gtaagtttgt cgttgtaggg caggtgttgg ggggctggga gatcgtcaac caggtagtga aactgtgcgg ctcgcgctgt gggactcccg tgagtcgtgc ctggatctct gactgcggtc agagtggtgg ctacatggca gaagagacgc aggaggccct acagggcgag cgcgctcagc atgtgatccc aggcaaggaa gttctcgact taattcaacc gcggtactaa

Details for LemaA.01375.a.B1.GE34441

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMW RLSRVALQSN RAALYMPYTP VATNPVVYFD ITAEGDALGR VSVELFRDVV PRTSENFRSL CTGERGYGQC LLYYKGTPFH RIIPGFVMQG GDILTKDGRS NVSVFGYPFP DESFEGKAGK HLPGTVGMAH SGPNQNGSQF FFNLGRNEQL DRKFVVVGQV LGGWEIVNQV VKLCGSRCGT PVSRAWISDC GQSGGYMAEE TQEALQGERA QHVIPGKEVL DLIQPRY

Validated NT Sequence

atcttactca cttagtaccg cggttgaatt aagtcgagaa cttccttgcc tgggatcaca tgctgagcgc gctcgccctg tagggcctcc tgcgtctctt ctgccatgta gccaccactc tgaccgcagt cagagatcca ggcacgactc acgggagtcc cacagcgcga gccgcacagt ttcactacct ggttgacgat ctcccagccc cccaacacct gccctacaac gacaaactta cgatcgagct gctcattgcg ccccagattg aagaaaaact gggatccatt ttggttcggc ccactgtgcg ccatgccgac agtgccgggc aagtgcttcc cggcctttcc ctcaaaagac tcatccggaa acggatatcc aaagacgcta acgttgctgc ggccgtcctt agtgagaatg tcgccgcctt gcatgacaaa ccctggaata atgcgatgaa acggggttcc cttgtagtac aacaagcact gaccatatcc gcgctcgcca gtgcacagcg agcggaagtt ctcgcttgtt ctcggcacaa cgtccctaaa cagctccacc gacacgcgcc ccagggcgtc gccttcggcg gtgatgtcaa aatagaccac cgggttagtc gctacggggg tgtacggcat gtagagcgcc gcgcgattcg actggagcgc cacacgcgag aggcgccaca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtggc gcctctcgcg tgtggcgctc cagtcgaatc gcgcggcgct ctacatgccg tacacccccg tagcgactaa cccggtggtc tattttgaca tcaccgccga aggcgacgcc ctggggcgcg tgtcggtgga gctgtttagg gacgttgtgc cgagaacaag cgagaacttc cgctcgctgt gcactggcga gcgcggatat ggtcagtgct tgttgtacta caagggaacc ccgtttcatc gcattattcc agggtttgtc atgcaaggcg gcgacattct cactaaggac ggccgcagca acgttagcgt ctttggatat ccgtttccgg atgagtcttt tgagggaaag gccgggaagc acttgcccgg cactgtcggc atggcgcaca gtgggccgaa ccaaaatgga tcccagtttt tcttcaatct ggggcgcaat gagcagctcg atcgtaagtt tgtcgttgta gggcaggtgt tggggggctg ggagatcgtc aaccaggtag tgaaactgtg cggctcgcgc tgtgggactc ccgtgagtcg tgcctggatc tctgactgcg gtcagagtgg tggctacatg gcagaagaga cgcaggaggc cctacagggc gagcgcgctc agcatgtgat cccaggcaag gaagttctcg acttaattca accgcggtac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat