LemaA.01332.a
aspartate carbamoyltransferase, putative
| CENTER ID: | LemaA.01332.a |
| ORGANISM: | Leishmania major Friedlin |
| ASSOCIATED DISEASE: | Cutaneous leishmaniasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.01332.a.AE1.GE43228 | full length | 1 | 327 | ||
| LemaA.01332.a.B1.GE34407 | full length | 1 | 327 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.01332.a.AE1.PS38618 | full length | 1 | 327 | ||
| LemaA.01332.a.AE1.PS38633 | full length | 1 | 327 | ||
| LemaA.01332.a.B1.PW35017 | full length | 1 | 327 |
Structures
7K46
DEPOSITED: 9/14/2020
DETERMINATION: XRay
CLONE: LemaA.01332.a.AE1.GE43228
PROTEIN: LemaA.01332.a.AE1.PS38633
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:LmjF16.0540 |
| RefSeq: | XP_001682132.1 |
| UniProt: | Q4QEW6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTFNPVASL KGQSVASAAQ FSRADIDALI QLALDMKTHI EAGKTIDTLR GRVMTPLFFE DSSRTLSSFC AAMMRLGGSV VNFKVETSSV NKGETLQDTI RTLDSYSDVL VLRHAKEEAL EQAMSVATHP IMNAGNGAGE HPTQALLDIL TIHAELGAVD GIAIALIGDL KKGRTVHSLL KLLAHNFALR KVYLIAPAGL EMPAEVLEHV ATDVVKRGIA IQQASGLTPE IVADCDVLYA TRLQKERFVA SAAGDADALT AFEASKASLL IDKARLAHAK AKMVVMHPLP RVDELSTDID DDPRAAYFRQ MRYGLFMRMA ILFSVLS
NT Sequence
atgcccacct tcaacccggt cgcctccctc aagggccaga gtgtggcctc cgctgcgcag ttctcgcgcg ctgacatcga cgccctcatc cagctcgcac tcgatatgaa gacacacatc gaggcaggga aaacgatcga caccctgcgc ggccgcgtca tgacgccgct gttcttcgag gacagctctc gcacgctgtc gagtttttgc gcggccatga tgcggctggg cggcagcgtc gtgaacttca aggtggagac gtcgtctgtg aacaagggtg aaacgctgca ggacaccatt cgcacgctgg actcgtacag tgacgtgctc gtgctccgcc acgctaagga ggaagcgctg gagcaggcga tgagtgtggc aacgcacccg atcatgaacg ccggcaacgg cgctggtgag cacccgactc aggcgttgct cgacatcctc acgattcacg ccgagctcgg cgccgtggac ggcattgcga tcgcgttgat cggggacctc aagaagggcc gcacggtgca ttcgctgctg aagctgctgg cgcacaactt cgcgctgagg aaggtgtacc tcatcgcccc cgcggggctg gagatgccgg cggaggtgct ggagcatgtc gcgacggacg tggtgaagcg cgggatcgcg attcagcagg cgtccggcct cactcctgag attgtggccg actgcgacgt gctctacgcg acgcgcctgc agaaggagcg ctttgttgcc tccgctgccg gtgacgccga cgccctgacc gcctttgagg cttctaaagc gagcctgctg atcgacaagg ctcgcctggc acacgcgaag gcgaagatgg ttgtcatgca cccgctgcct cgcgtggatg agctcagcac cgacattgac gacgacccgc gtgcggcgta tttccgacag atgcgctacg gcctcttcat gcgcatggct atcctcttca gcgtgctttc ttga
Details for LemaA.01332.a.AE1.GE43228
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 32 |
Validated AA Sequence
MMXAFAPVAS LKGXSVASAA XVSGADIDAL IXIALDMKTH LEAGKTIDTL RGRVMTPLFF EDSSRTLSRF CAAMMRVGGS VXNFXVETSX VXXGETVQDT IRTLXLXX
Validated NT Sequence
atgatgnccg ccttcgcccc ggtcgcctcc ctcaagggcc anagtgtggc ctccgctgcg cangtctcgg gcgctgacat cgacgccctc atccanatcg cactcgatat gaagacacac ctcgaggcag ggaaaacgat cgacaccctg cgcggccgcg tcatgacncc gctgttcttc gaggacagct ctcgcacgct gtcgaggttt tgcgcggcca tgatgcgggt gggcggcagc gtccngaact tcnaggtgga gacgtcgnct gtgaannang gtgaaacggt gcaggacacc attcgcacgc ttgnactcnt acng
Expected Protein Sequence
MPTFNPVASL KGQSVASAAQ FSRADIDALI QLALDMKTHI EAGKTIDTLR GRVMTPLFFE DSSRTLSSFC AAMMRLGGSV VNFKVETSSV NKGETLQDTI RTLDSYSDVL VLRHAKEEAL EQAMSVATHP IMNAGNGAGE HPTQALLDIL TIHAELGAVD GIAIALIGDL KKGRTVHSLL KLLAHNFALR KVYLIAPAGL EMPAEVLEHV ATDVVKRGIA IQQASGLTPE IVADCDVLYA TRLQKERFVA SAAGDADALT AFEASKASLL IDKARLAHAK AKMVVMHPLP RVDELSTDID DDPRAAYFRQ MRYGLFMRMA ILFSVLSGHH HHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gcccaccttc aacccggtcg cctccctcaa gggccagagt gtggcctccg ctgcgcagtt ctcgcgcgct gacatcgacg ccctcatcca gctcgcactc gatatgaaga cacacatcga ggcagggaaa acgatcgaca ccctgcgcgg ccgcgtcatg acgccgctgt tcttcgagga cagctctcgc acgctgtcga gtttttgcgc ggccatgatg cggctgggcg gcagcgtcgt gaacttcaag gtggagacgt cgtctgtgaa caagggtgaa acgctgcagg acaccattcg cacgctggac tcgtacagtg acgtgctcgt gctccgccac gctaaggagg aagcgctgga gcaggcgatg agtgtggcaa cgcacccgat catgaacgcc ggcaacggcg ctggtgagca cccgactcag gcgttgctcg acatcctcac gattcacgcc gagctcggcg ccgtggacgg cattgcgatc gcgttgatcg gggacctcaa gaagggccgc acggtgcatt cgctgctgaa gctgctggcg cacaacttcg cgctgaggaa ggtgtacctc atcgcccccg cggggctgga gatgccggcg gaggtgctgg agcatgtcgc gacggacgtg gtgaagcgcg ggatcgcgat tcagcaggcg tccggcctca ctcctgagat tgtggccgac tgcgacgtgc tctacgcgac gcgcctgcag aaggagcgct ttgttgcctc cgctgccggt gacgccgacg ccctgaccgc ctttgaggct tctaaagcga gcctgctgat cgacaaggct cgcctggcac acgcgaaggc gaagatggtt gtcatgcacc cgctgcctcg cgtggatgag ctcagcaccg acattgacga cgacccgcgt gcggcgtatt tccgacagat gcgctacggc ctcttcatgc gcatggctat cctcttcagc gtgctttctg ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for LemaA.01332.a.B1.GE34407
| HARVESTED ON: | 2/6/2012 |
| SEQUENCED ON: | 2/21/2012 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MPTXNPVASL KGQSVASAAQ FSRADIDALI QLALDMKTHI EAGKTIDTLR GRVMTPLFFE DSSRTLSSFC AAMMRLGGSV VNFKVETSSV NKGETLQDTI RTLDSYSDVL VLRHAKEEAL EQAMSVATHP IMNAGNGAGE HPTQALLDIL TIHAELGAVD GIAIALIGDL KKGRTVHSLL KLLAHNFALR KVYLIAPAGL EMPAEVLEHV ATDVVKRGIA IQQASGLTPE IVADCDVLYA TRLQKERFVA SAAGDADALT AFEASKASLL IDKARLAHAK AKMVVMHPLP RVDELSTDID DDPRAAYFRQ MRYGLFM
Validated NT Sequence
gcgcatgaag aggccgtagc gcatctgtcg gaaatacgcc gcacgcgggt cgtcgtcaat gtcggtgctg agctcatcca cgcgaggcag cgggtgcatg acaaccatct tcgccttcgc gtgtgccagg cgagccttgt cgatcagcag gctcgcttta gaagcctcaa aggcggtcag ggcgtcggcg tcaccggcag cggaggcaac aaagcgctcc ttctgcaggc gcgtcgcgta gagcacgtcg cagtcggcca caatctcagg agtgaggccg gacgcctgct gaatcgcgat cccgcgcttc accacgtccg tcgcgacatg ctccagcacc tccgccggca tctccagccc cgcgggggcg atgaggtaca ccttcctcag cgcgaagttg tgcgccagca gcttcagcag cgaatgcacc gtgcggccct tcttgaggtc cccgatcaac gcgatcgcaa tgccgtccac ggcgccgagc tcggcgtgaa tcgtgaggat gtcgagcaac gcctgagtcg ggtgctcacc agcgccgttg ccggcgttca tgatcgggtg cgttgccaca ctcatcgcct gctccagcgc ttcctcctta gcgtggcgga gcacgagcac gtcactgtac gagtccagcg tgcgaatggt gtcctgcagc gtttcaccct tgttcacaga cgacgtctcc accttgaagt tcacgacgct gccgcccagc cgcatcatgg ccgcgcaaaa actcgacagc gtgcgagagc tgtcctcgaa gaacagcggc gtcatgacgc ggccgcgcag ggtgtcgatc gttttccctg cctcgatgtg tgtcttcata tcgagtgcga gctggatgag ggcgtcgatg tcagcncgcg agaactgcgc agcggaggcc acactctggc ccttgaggga ggcgaccggg ttnaaggtgg gcatatggtg gtgg
Expected Protein Sequence
MAHHHHHHMP TFNPVASLKG QSVASAAQFS RADIDALIQL ALDMKTHIEA GKTIDTLRGR VMTPLFFEDS SRTLSSFCAA MMRLGGSVVN FKVETSSVNK GETLQDTIRT LDSYSDVLVL RHAKEEALEQ AMSVATHPIM NAGNGAGEHP TQALLDILTI HAELGAVDGI AIALIGDLKK GRTVHSLLKL LAHNFALRKV YLIAPAGLEM PAEVLEHVAT DVVKRGIAIQ QASGLTPEIV ADCDVLYATR LQKERFVASA AGDADALTAF EASKASLLID KARLAHAKAK MVVMHPLPRV DELSTDIDDD PRAAYFRQMR YGLFMRMAIL FSVLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca ccttcaaccc ggtcgcctcc ctcaagggcc agagtgtggc ctccgctgcg cagttctcgc gcgctgacat cgacgccctc atccagctcg cactcgatat gaagacacac atcgaggcag ggaaaacgat cgacaccctg cgcggccgcg tcatgacgcc gctgttcttc gaggacagct ctcgcacgct gtcgagtttt tgcgcggcca tgatgcggct gggcggcagc gtcgtgaact tcaaggtgga gacgtcgtct gtgaacaagg gtgaaacgct gcaggacacc attcgcacgc tggactcgta cagtgacgtg ctcgtgctcc gccacgctaa ggaggaagcg ctggagcagg cgatgagtgt ggcaacgcac ccgatcatga acgccggcaa cggcgctggt gagcacccga ctcaggcgtt gctcgacatc ctcacgattc acgccgagct cggcgccgtg gacggcattg cgatcgcgtt gatcggggac ctcaagaagg gccgcacggt gcattcgctg ctgaagctgc tggcgcacaa cttcgcgctg aggaaggtgt acctcatcgc ccccgcgggg ctggagatgc cggcggaggt gctggagcat gtcgcgacgg acgtggtgaa gcgcgggatc gcgattcagc aggcgtccgg cctcactcct gagattgtgg ccgactgcga cgtgctctac gcgacgcgcc tgcagaagga gcgctttgtt gcctccgctg ccggtgacgc cgacgccctg accgcctttg aggcttctaa agcgagcctg ctgatcgaca aggctcgcct ggcacacgcg aaggcgaaga tggttgtcat gcacccgctg cctcgcgtgg atgagctcag caccgacatt gacgacgacc cgcgtgcggc gtatttccga cagatgcgct acggcctctt catgcgcatg gctatcctct tcagcgtgct ttcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LemaA.01332.a.AE1.PS38618
| PURIFICATION DATe: | 11/5/2019 |
| CONCENTRATION: | 87.42mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 32 |
Protocol Notes
notes unavailable
Validated AA Sequence
MMXAFAPVAS LKGXSVASAA XVSGADIDAL IXIALDMKTH LEAGKTIDTL RGRVMTPLFF EDSSRTLSRF CAAMMRVGGS VXNFXVETSX VXXGETVQDT IRTLXLXX
Validated NT Sequence
atgatgnccg ccttcgcccc ggtcgcctcc ctcaagggcc anagtgtggc ctccgctgcg cangtctcgg gcgctgacat cgacgccctc atccanatcg cactcgatat gaagacacac ctcgaggcag ggaaaacgat cgacaccctg cgcggccgcg tcatgacncc gctgttcttc gaggacagct ctcgcacgct gtcgaggttt tgcgcggcca tgatgcgggt gggcggcagc gtccngaact tcnaggtgga gacgtcgnct gtgaannang gtgaaacggt gcaggacacc attcgcacgc ttgnactcnt acng
Expressed Protein Sequence
MPTFNPVASL KGQSVASAAQ FSRADIDALI QLALDMKTHI EAGKTIDTLR GRVMTPLFFE DSSRTLSSFC AAMMRLGGSV VNFKVETSSV NKGETLQDTI RTLDSYSDVL VLRHAKEEAL EQAMSVATHP IMNAGNGAGE HPTQALLDIL TIHAELGAVD GIAIALIGDL KKGRTVHSLL KLLAHNFALR KVYLIAPAGL EMPAEVLEHV ATDVVKRGIA IQQASGLTPE IVADCDVLYA TRLQKERFVA SAAGDADALT AFEASKASLL IDKARLAHAK AKMVVMHPLP RVDELSTDID DDPRAAYFRQ MRYGLFMRMA ILFSVLSGHH HHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GCCCACCTTC AACCCGGTCG CCTCCCTCAA GGGCCAGAGT GTGGCCTCCG CTGCGCAGTT CTCGCGCGCT GACATCGACG CCCTCATCCA GCTCGCACTC GATATGAAGA CACACATCGA GGCAGGGAAA ACGATCGACA CCCTGCGCGG CCGCGTCATG ACGCCGCTGT TCTTCGAGGA CAGCTCTCGC ACGCTGTCGA GTTTTTGCGC GGCCATGATG CGGCTGGGCG GCAGCGTCGT GAACTTCAAG GTGGAGACGT CGTCTGTGAA CAAGGGTGAA ACGCTGCAGG ACACCATTCG CACGCTGGAC TCGTACAGTG ACGTGCTCGT GCTCCGCCAC GCTAAGGAGG AAGCGCTGGA GCAGGCGATG AGTGTGGCAA CGCACCCGAT CATGAACGCC GGCAACGGCG CTGGTGAGCA CCCGACTCAG GCGTTGCTCG ACATCCTCAC GATTCACGCC GAGCTCGGCG CCGTGGACGG CATTGCGATC GCGTTGATCG GGGACCTCAA GAAGGGCCGC ACGGTGCATT CGCTGCTGAA GCTGCTGGCG CACAACTTCG CGCTGAGGAA GGTGTACCTC ATCGCCCCCG CGGGGCTGGA GATGCCGGCG GAGGTGCTGG AGCATGTCGC GACGGACGTG GTGAAGCGCG GGATCGCGAT TCAGCAGGCG TCCGGCCTCA CTCCTGAGAT TGTGGCCGAC TGCGACGTGC TCTACGCGAC GCGCCTGCAG AAGGAGCGCT TTGTTGCCTC CGCTGCCGGT GACGCCGACG CCCTGACCGC CTTTGAGGCT TCTAAAGCGA GCCTGCTGAT CGACAAGGCT CGCCTGGCAC ACGCGAAGGC GAAGATGGTT GTCATGCACC CGCTGCCTCG CGTGGATGAG CTCAGCACCG ACATTGACGA CGACCCGCGT GCGGCGTATT TCCGACAGAT GCGCTACGGC CTCTTCATGC GCATGGCTAT CCTCTTCAGC GTGCTTTCTG GGCACCACCA TCATCATCAT TAACGGATCC GAATTCGAGC TCCGTCGACA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT
Details for LemaA.01332.a.AE1.PS38633
| PURIFICATION DATe: | 1/14/2020 |
| CONCENTRATION: | 13.16mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 32 |
Protocol Notes
notes unavailable
Validated AA Sequence
MMXAFAPVAS LKGXSVASAA XVSGADIDAL IXIALDMKTH LEAGKTIDTL RGRVMTPLFF EDSSRTLSRF CAAMMRVGGS VXNFXVETSX VXXGETVQDT IRTLXLXX
Validated NT Sequence
atgatgnccg ccttcgcccc ggtcgcctcc ctcaagggcc anagtgtggc ctccgctgcg cangtctcgg gcgctgacat cgacgccctc atccanatcg cactcgatat gaagacacac ctcgaggcag ggaaaacgat cgacaccctg cgcggccgcg tcatgacncc gctgttcttc gaggacagct ctcgcacgct gtcgaggttt tgcgcggcca tgatgcgggt gggcggcagc gtccngaact tcnaggtgga gacgtcgnct gtgaannang gtgaaacggt gcaggacacc attcgcacgc ttgnactcnt acng
Expressed Protein Sequence
MPTFNPVASL KGQSVASAAQ FSRADIDALI QLALDMKTHI EAGKTIDTLR GRVMTPLFFE DSSRTLSSFC AAMMRLGGSV VNFKVETSSV NKGETLQDTI RTLDSYSDVL VLRHAKEEAL EQAMSVATHP IMNAGNGAGE HPTQALLDIL TIHAELGAVD GIAIALIGDL KKGRTVHSLL KLLAHNFALR KVYLIAPAGL EMPAEVLEHV ATDVVKRGIA IQQASGLTPE IVADCDVLYA TRLQKERFVA SAAGDADALT AFEASKASLL IDKARLAHAK AKMVVMHPLP RVDELSTDID DDPRAAYFRQ MRYGLFMRMA ILFSVLSGHH HHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GCCCACCTTC AACCCGGTCG CCTCCCTCAA GGGCCAGAGT GTGGCCTCCG CTGCGCAGTT CTCGCGCGCT GACATCGACG CCCTCATCCA GCTCGCACTC GATATGAAGA CACACATCGA GGCAGGGAAA ACGATCGACA CCCTGCGCGG CCGCGTCATG ACGCCGCTGT TCTTCGAGGA CAGCTCTCGC ACGCTGTCGA GTTTTTGCGC GGCCATGATG CGGCTGGGCG GCAGCGTCGT GAACTTCAAG GTGGAGACGT CGTCTGTGAA CAAGGGTGAA ACGCTGCAGG ACACCATTCG CACGCTGGAC TCGTACAGTG ACGTGCTCGT GCTCCGCCAC GCTAAGGAGG AAGCGCTGGA GCAGGCGATG AGTGTGGCAA CGCACCCGAT CATGAACGCC GGCAACGGCG CTGGTGAGCA CCCGACTCAG GCGTTGCTCG ACATCCTCAC GATTCACGCC GAGCTCGGCG CCGTGGACGG CATTGCGATC GCGTTGATCG GGGACCTCAA GAAGGGCCGC ACGGTGCATT CGCTGCTGAA GCTGCTGGCG CACAACTTCG CGCTGAGGAA GGTGTACCTC ATCGCCCCCG CGGGGCTGGA GATGCCGGCG GAGGTGCTGG AGCATGTCGC GACGGACGTG GTGAAGCGCG GGATCGCGAT TCAGCAGGCG TCCGGCCTCA CTCCTGAGAT TGTGGCCGAC TGCGACGTGC TCTACGCGAC GCGCCTGCAG AAGGAGCGCT TTGTTGCCTC CGCTGCCGGT GACGCCGACG CCCTGACCGC CTTTGAGGCT TCTAAAGCGA GCCTGCTGAT CGACAAGGCT CGCCTGGCAC ACGCGAAGGC GAAGATGGTT GTCATGCACC CGCTGCCTCG CGTGGATGAG CTCAGCACCG ACATTGACGA CGACCCGCGT GCGGCGTATT TCCGACAGAT GCGCTACGGC CTCTTCATGC GCATGGCTAT CCTCTTCAGC GTGCTTTCTG GGCACCACCA TCATCATCAT TAACGGATCC GAATTCGAGC TCCGTCGACA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT
Details for LemaA.01332.a.B1.PW35017
| PURIFICATION DATe: | 3/19/2012 |
| CONCENTRATION: | 28.69mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPTXNPVASL KGQSVASAAQ FSRADIDALI QLALDMKTHI EAGKTIDTLR GRVMTPLFFE DSSRTLSSFC AAMMRLGGSV VNFKVETSSV NKGETLQDTI RTLDSYSDVL VLRHAKEEAL EQAMSVATHP IMNAGNGAGE HPTQALLDIL TIHAELGAVD GIAIALIGDL KKGRTVHSLL KLLAHNFALR KVYLIAPAGL EMPAEVLEHV ATDVVKRGIA IQQASGLTPE IVADCDVLYA TRLQKERFVA SAAGDADALT AFEASKASLL IDKARLAHAK AKMVVMHPLP RVDELSTDID DDPRAAYFRQ MRYGLFM
Validated NT Sequence
gcgcatgaag aggccgtagc gcatctgtcg gaaatacgcc gcacgcgggt cgtcgtcaat gtcggtgctg agctcatcca cgcgaggcag cgggtgcatg acaaccatct tcgccttcgc gtgtgccagg cgagccttgt cgatcagcag gctcgcttta gaagcctcaa aggcggtcag ggcgtcggcg tcaccggcag cggaggcaac aaagcgctcc ttctgcaggc gcgtcgcgta gagcacgtcg cagtcggcca caatctcagg agtgaggccg gacgcctgct gaatcgcgat cccgcgcttc accacgtccg tcgcgacatg ctccagcacc tccgccggca tctccagccc cgcgggggcg atgaggtaca ccttcctcag cgcgaagttg tgcgccagca gcttcagcag cgaatgcacc gtgcggccct tcttgaggtc cccgatcaac gcgatcgcaa tgccgtccac ggcgccgagc tcggcgtgaa tcgtgaggat gtcgagcaac gcctgagtcg ggtgctcacc agcgccgttg ccggcgttca tgatcgggtg cgttgccaca ctcatcgcct gctccagcgc ttcctcctta gcgtggcgga gcacgagcac gtcactgtac gagtccagcg tgcgaatggt gtcctgcagc gtttcaccct tgttcacaga cgacgtctcc accttgaagt tcacgacgct gccgcccagc cgcatcatgg ccgcgcaaaa actcgacagc gtgcgagagc tgtcctcgaa gaacagcggc gtcatgacgc ggccgcgcag ggtgtcgatc gttttccctg cctcgatgtg tgtcttcata tcgagtgcga gctggatgag ggcgtcgatg tcagcncgcg agaactgcgc agcggaggcc acactctggc ccttgaggga ggcgaccggg ttnaaggtgg gcatatggtg gtgg
Expressed Protein Sequence
MAHHHHHHMP TFNPVASLKG QSVASAAQFS RADIDALIQL ALDMKTHIEA GKTIDTLRGR VMTPLFFEDS SRTLSSFCAA MMRLGGSVVN FKVETSSVNK GETLQDTIRT LDSYSDVLVL RHAKEEALEQ AMSVATHPIM NAGNGAGEHP TQALLDILTI HAELGAVDGI AIALIGDLKK GRTVHSLLKL LAHNFALRKV YLIAPAGLEM PAEVLEHVAT DVVKRGIAIQ QASGLTPEIV ADCDVLYATR LQKERFVASA AGDADALTAF EASKASLLID KARLAHAKAK MVVMHPLPRV DELSTDIDDD PRAAYFRQMR YGLFMRMAIL FSVLS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCA CCTTCAACCC GGTCGCCTCC CTCAAGGGCC AGAGTGTGGC CTCCGCTGCG CAGTTCTCGC GCGCTGACAT CGACGCCCTC ATCCAGCTCG CACTCGATAT GAAGACACAC ATCGAGGCAG GGAAAACGAT CGACACCCTG CGCGGCCGCG TCATGACGCC GCTGTTCTTC GAGGACAGCT CTCGCACGCT GTCGAGTTTT TGCGCGGCCA TGATGCGGCT GGGCGGCAGC GTCGTGAACT TCAAGGTGGA GACGTCGTCT GTGAACAAGG GTGAAACGCT GCAGGACACC ATTCGCACGC TGGACTCGTA CAGTGACGTG CTCGTGCTCC GCCACGCTAA GGAGGAAGCG CTGGAGCAGG CGATGAGTGT GGCAACGCAC CCGATCATGA ACGCCGGCAA CGGCGCTGGT GAGCACCCGA CTCAGGCGTT GCTCGACATC CTCACGATTC ACGCCGAGCT CGGCGCCGTG GACGGCATTG CGATCGCGTT GATCGGGGAC CTCAAGAAGG GCCGCACGGT GCATTCGCTG CTGAAGCTGC TGGCGCACAA CTTCGCGCTG AGGAAGGTGT ACCTCATCGC CCCCGCGGGG CTGGAGATGC CGGCGGAGGT GCTGGAGCAT GTCGCGACGG ACGTGGTGAA GCGCGGGATC GCGATTCAGC AGGCGTCCGG CCTCACTCCT GAGATTGTGG CCGACTGCGA CGTGCTCTAC GCGACGCGCC TGCAGAAGGA GCGCTTTGTT GCCTCCGCTG CCGGTGACGC CGACGCCCTG ACCGCCTTTG AGGCTTCTAA AGCGAGCCTG CTGATCGACA AGGCTCGCCT GGCACACGCG AAGGCGAAGA TGGTTGTCAT GCACCCGCTG CCTCGCGTGG ATGAGCTCAG CACCGACATT GACGACGACC CGCGTGCGGC GTATTTCCGA CAGATGCGCT ACGGCCTCTT CATGCGCATG GCTATCCTCT TCAGCGTGCT TTCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT