SSGCID: Target Page

LemaA.01291.b

adenylate kinase, putative

CENTER ID:	LemaA.01291.b
ORGANISM:	Leishmania major Friedlin
ASSOCIATED DISEASE:	Cutaneous leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemaA.01291.b.B1.GE34401	full length		1	218

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmjF34.0110
RefSeq:	XP_001686101.1
UniProt:	Q4Q3J7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIVLMGAPG CGKGTQSPYI QDRYGLCHLS TGDMLRDAVA RKTANGKLAK DAMDSGKLVS DDIVFGIVKD SIKNPECRYG YILDGYPRTL KQAQMMEDAG EKIDKVIEFS VPDEVILERT SGRWIHKSSG RTYHEVFRPP KTPGKDDITG EDLYQRPDDR REVCEKRLDI YKNETRPLAD YFTKEGVYST INANQAIDEV RKIIAALLDP IGIATGLK

NT Sequence

atgaagattg tgctcatggg cgcccccggc tgcggtaaag gtacacagag cccgtacata caggaccggt acggtctgtg ccatctgtcg acgggcgata tgctgcgcga cgcggtggcg cggaagactg caaacggaaa actggcgaag gacgccatgg attcgggcaa gcttgtcagt gacgacattg tttttgggat tgtgaaggat agcattaaga accctgagtg ccgctacggc tacatcctag acggctaccc gcgcacgctg aagcaggcgc aaatgatgga ggacgccggg gagaagatag acaaggtaat cgagttcagc gttccggacg aggtcatcct ggagcggacg agtgggcggt ggattcacaa gtcgagcggg cgcacgtacc acgaggtgtt ccgccctccg aagacgcccg gcaaggatga cataacgggt gaggacctct accaacgccc cgacgatcgc cgcgaggtgt gcgagaagcg cctggatatc tataagaatg agactcgtcc tctcgccgac tacttcacca aggagggcgt gtattcgacg atcaacgcca accaagcgat cgacgaagtt cgaaagatta tcgccgcttt gttggacccc attggcattg caacgggcct caagtag

Details for LemaA.01291.b.B1.GE34401

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK IVLMGAPGCG KGTQSPYIQD RYGLCHLSTG DMLRDAVARK TANGKLAKDA MDSGKLVSDD IVFGIVKDSI KNPECRYGYI LDGYPRTLKQ AQMMEDAGEK IDKVIEFSVP DEVILERTSG RWIHKSSGRT YHEVFRPPKT PGKDDITGED LYQRPDDRRE VCEKRLDIYK NETRPLADYF TKEGVYSTIN ANQAIDEVRK IIAALLDPIG IATGLK

Validated NT Sequence

atcttactca cttacttgag gcccgttgca atgccaatgg ggtccaacaa agcggcgata atctttcgaa cttcgtcgat cgcttggttg gcgttgatcg tcgaatacac gccctccttg gtgaagtagt cggcgagagg acgagtctca ttcttataga tatccaggcg cttctcgcac acctcgcggc gatcgtcggg gcgttggtag aggtcctcac ccgttatgtc atccttgccg ggcgtcttcg gagggcggaa cacctcgtgg tacgtgcgcc cgctcgactt gtgaatccac cgcccactcg tccgctccag gatgacctcg tccggaacgc tgaactcgat taccttgtct atcttctccc cggcgtcctc catcatttgc gcctgcttca gcgtgcgcgg gtagccgtct aggatgtagc cgtagcggca ctcagggttc ttaatgctat ccttcacaat cccaaaaaca atgtcgtcac tgacaagctt gcccgaatcc atggcgtcct tcgccagttt tccgtttgca gtcttccgcg ccaccgcgtc gcgcagcata tcgcccgtcg acagatggca cagaccgtac cggtcctgta tgtacgggct ctgtgtacct ttaccgcagc cgggggcgcc catgagcaca atcttcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga ttgtgctcat gggcgccccc ggctgcggta aaggtacaca gagcccgtac atacaggacc ggtacggtct gtgccatctg tcgacgggcg atatgctgcg cgacgcggtg gcgcggaaga ctgcaaacgg aaaactggcg aaggacgcca tggattcggg caagcttgtc agtgacgaca ttgtttttgg gattgtgaag gatagcatta agaaccctga gtgccgctac ggctacatcc tagacggcta cccgcgcacg ctgaagcagg cgcaaatgat ggaggacgcc ggggagaaga tagacaaggt aatcgagttc agcgttccgg acgaggtcat cctggagcgg acgagtgggc ggtggattca caagtcgagc gggcgcacgt accacgaggt gttccgccct ccgaagacgc ccggcaagga tgacataacg ggtgaggacc tctaccaacg ccccgacgat cgccgcgagg tgtgcgagaa gcgcctggat atctataaga atgagactcg tcctctcgcc gactacttca ccaaggaggg cgtgtattcg acgatcaacg ccaaccaagc gatcgacgaa gttcgaaaga ttatcgccgc tttgttggac cccattggca ttgcaacggg cctcaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at