LemaA.01199.b
iron superoxide dismutase, putative
| CENTER ID: | LemaA.01199.b |
| ORGANISM: | Leishmania major Friedlin |
| ASSOCIATED DISEASE: | Cutaneous leishmaniasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.01199.b.A2.GE34364 | mature protein | 22 | 230 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LemaA.01199.b.A2.PW34974 | mature protein | 22 | 230 |
Structures
4F2N
DEPOSITED: 5/8/2012
DETERMINATION: XRay
CLONE: LemaA.01199.b.A2.GE34364
PROTEIN: LemaA.01199.b.A2.PW34974
Publications by SSGCID
Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures.
Anupama A, Cestari I, Davies DR, Edwards TE, Fairman JW, Moretti NS, Myler PJ, Phan IQ, Schenkman S, Shanmugam D, Stuart K
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 615-21
PMID: 25961325; PMCID: PMC4427173
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:LmjF08.0290 |
| OrthoMCL: | OG5_126676 |
| RefSeq: | XP_001681058.1 |
| UniProt: | Q4QIE0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFGRAPMKAA TATAAVGFSC LCYHTLPHLR YPAELPTLGF NYKDGIQPVM SPRQLELHYS KHHSAYVDKL NTLGKGYEGK TIEEIILATT GINESKVMFN QAAQHFNHSF FWKCLSPGGK PMPKTLENAI AKQFGSVDDF MVSFQQAGVN NFGSGWTWLC VDPQTKELLI DSTSNAGCPL TSGLRPIFTA DVWEHAYYKD FENRRADYLK ELWQIVDWEF VCHMYERATK
NT Sequence
atgttcggcc gcgccccgat gaaggccgcc acggccaccg ctgccgtcgg gttttcgtgc ctgtgctacc atacccttcc tcatcttcgg tacccggccg agctgccgac gcttggcttc aactataagg acggcatcca gcccgtcatg agcccccgtc agctggagct gcactacagc aagcaccaca gcgcgtacgt ggacaagttg aacacgctcg gcaagggcta tgaggggaag acgattgagg agatcatctt ggcgaccacc ggcatcaatg aaagcaaggt catgttcaac caggccgccc agcacttcaa ccattccttc ttctggaagt gcttgtcgcc tggtggcaag ccgatgccga agacgctcga gaatgcaatc gcgaagcagt tcggaagcgt cgacgacttc atggtgtcct tccagcaggc cggcgtgaac aactttggct ctggctggac gtggctctgc gtcgatcccc agacgaagga gctgctcatc gacagcacga gcaacgcggg ctgcccgctg acctctggct tgcgccccat cttcacagct gatgtatggg agcatgccta ctacaaggac ttcgagaacc gtcgcgcgga ctacctgaaa gagctctggc agatcgtcga ctgggaattt gtctgccata tgtatgagag ggccacgaag taa
Details for LemaA.01199.b.A2.GE34364
| HARVESTED ON: | 1/10/2012 |
| SEQUENCED ON: | 1/25/2012 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCYHTLPHL RYPAELPTLG FNYKDGIQPV MSPRQLELHY SKHHSAYVDK LNTLGKGYEG KTIEEIILAT TGINESKVMF NQAAQHFNHS FFWKCLSPGG KPMPKTLENA IAKQFGSVDD FMVSFQQAGV NNFGSGWTWL CVDPQTKELL IDSTSNAGCP LTSGLRPIFT ADVWEHAYYK DFENRRADYL KELWQIVDWE FVCHMYERAT K
Validated NT Sequence
ttattacttc gtggccctct catacatatg gcagacaaat tcccagtcga cgatctgcca gagctctttc aggtagtccg cgcgacggtt ctcgaagtcc ttgtagtagg catgctccca tacatcagct gtgaagatgg ggcgcaagcc agaggtcagc gggcagcccg cgttgctcgt gctgtcgatg agcagctcct tcgtctgggg atcgacgcag agccacgtcc agccagagcc aaagttgttc acgccggcct gctggaagga caccatgaag tcgtcgacgc ttccgaactg cttcgcgatt gcattctcga gcgtcttcgg catcggcttg ccaccaggcg acaagcactt ccagaagaag gaatggttga agtgctgggc ggcctggttg aacatgacct tgctttcatt gatgccggtg gtcgccaaga tgatctcctc aatcgtcttc ccctcatagc ccttgccgag cgtgttcaac ttgtccacgt acgcgctgtg gtgcttgctg tagtgcagct ccagctgacg ggggctcatg acgggctgga tgccgtcctt atagttgaag ccaagcgtcg gcagctcggc cgggtaccga agatgaggaa gggtatggta gcacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCYHTLPHL RYPAELPTLG FNYKDGIQPV MSPRQLELHY SKHHSAYVDK LNTLGKGYEG KTIEEIILAT TGINESKVMF NQAAQHFNHS FFWKCLSPGG KPMPKTLENA IAKQFGSVDD FMVSFQQAGV NNFGSGWTWL CVDPQTKELL IDSTSNAGCP LTSGLRPIFT ADVWEHAYYK DFENRRADYL KELWQIVDWE FVCHMYERAT K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgctaccat acccttcctc atcttcggta cccggccgag ctgccgacgc ttggcttcaa ctataaggac ggcatccagc ccgtcatgag cccccgtcag ctggagctgc actacagcaa gcaccacagc gcgtacgtgg acaagttgaa cacgctcggc aagggctatg aggggaagac gattgaggag atcatcttgg cgaccaccgg catcaatgaa agcaaggtca tgttcaacca ggccgcccag cacttcaacc attccttctt ctggaagtgc ttgtcgcctg gtggcaagcc gatgccgaag acgctcgaga atgcaatcgc gaagcagttc ggaagcgtcg acgacttcat ggtgtccttc cagcaggccg gcgtgaacaa ctttggctct ggctggacgt ggctctgcgt cgatccccag acgaaggagc tgctcatcga cagcacgagc aacgcgggct gcccgctgac ctctggcttg cgccccatct tcacagctga tgtatgggag catgcctact acaaggactt cgagaaccgt cgcgcggact acctgaaaga gctctggcag atcgtcgact gggaatttgt ctgccatatg tatgagaggg ccacgaagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for LemaA.01199.b.A2.PW34974
| PURIFICATION DATe: | 2/24/2012 |
| CONCENTRATION: | 35.33mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCYHTLPHL RYPAELPTLG FNYKDGIQPV MSPRQLELHY SKHHSAYVDK LNTLGKGYEG KTIEEIILAT TGINESKVMF NQAAQHFNHS FFWKCLSPGG KPMPKTLENA IAKQFGSVDD FMVSFQQAGV NNFGSGWTWL CVDPQTKELL IDSTSNAGCP LTSGLRPIFT ADVWEHAYYK DFENRRADYL KELWQIVDWE FVCHMYERAT K
Validated NT Sequence
ttattacttc gtggccctct catacatatg gcagacaaat tcccagtcga cgatctgcca gagctctttc aggtagtccg cgcgacggtt ctcgaagtcc ttgtagtagg catgctccca tacatcagct gtgaagatgg ggcgcaagcc agaggtcagc gggcagcccg cgttgctcgt gctgtcgatg agcagctcct tcgtctgggg atcgacgcag agccacgtcc agccagagcc aaagttgttc acgccggcct gctggaagga caccatgaag tcgtcgacgc ttccgaactg cttcgcgatt gcattctcga gcgtcttcgg catcggcttg ccaccaggcg acaagcactt ccagaagaag gaatggttga agtgctgggc ggcctggttg aacatgacct tgctttcatt gatgccggtg gtcgccaaga tgatctcctc aatcgtcttc ccctcatagc ccttgccgag cgtgttcaac ttgtccacgt acgcgctgtg gtgcttgctg tagtgcagct ccagctgacg ggggctcatg acgggctgga tgccgtcctt atagttgaag ccaagcgtcg gcagctcggc cgggtaccga agatgaggaa gggtatggta gcacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCYHTLPHL RYPAELPTLG FNYKDGIQPV MSPRQLELHY SKHHSAYVDK LNTLGKGYEG KTIEEIILAT TGINESKVMF NQAAQHFNHS FFWKCLSPGG KPMPKTLENA IAKQFGSVDD FMVSFQQAGV NNFGSGWTWL CVDPQTKELL IDSTSNAGCP LTSGLRPIFT ADVWEHAYYK DFENRRADYL KELWQIVDWE FVCHMYERAT K
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCTACCAT ACCCTTCCTC ATCTTCGGTA CCCGGCCGAG CTGCCGACGC TTGGCTTCAA CTATAAGGAC GGCATCCAGC CCGTCATGAG CCCCCGTCAG CTGGAGCTGC ACTACAGCAA GCACCACAGC GCGTACGTGG ACAAGTTGAA CACGCTCGGC AAGGGCTATG AGGGGAAGAC GATTGAGGAG ATCATCTTGG CGACCACCGG CATCAATGAA AGCAAGGTCA TGTTCAACCA GGCCGCCCAG CACTTCAACC ATTCCTTCTT CTGGAAGTGC TTGTCGCCTG GTGGCAAGCC GATGCCGAAG ACGCTCGAGA ATGCAATCGC GAAGCAGTTC GGAAGCGTCG ACGACTTCAT GGTGTCCTTC CAGCAGGCCG GCGTGAACAA CTTTGGCTCT GGCTGGACGT GGCTCTGCGT CGATCCCCAG ACGAAGGAGC TGCTCATCGA CAGCACGAGC AACGCGGGCT GCCCGCTGAC CTCTGGCTTG CGCCCCATCT TCACAGCTGA TGTATGGGAG CATGCCTACT ACAAGGACTT CGAGAACCGT CGCGCGGACT ACCTGAAAGA GCTCTGGCAG ATCGTCGACT GGGAATTTGT CTGCCATATG TATGAGAGGG CCACGAAGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A